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Protein

Drebrin-like protein

Gene

Dbnl

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein that binds F-actin and DNM1, and thereby plays a role in receptor-mediated endocytosis. Required for the formation of organized podosome rosettes. May act as a common effector of antigen receptor-signaling pathways in leukocytes. Acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes (By similarity). Plays a role in the reorganization of the actin cytoskeleton, formation of cell projections, such as neurites, in neuron morphogenesis and synapse formation via its interaction with WASL and COBL. Does not bind G-actin and promote actin polymerization by itself.By similarity4 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActin-binding
Biological processAdaptive immunity, Endocytosis, Immunity, Transport

Enzyme and pathway databases

ReactomeiR-RNO-264870. Caspase-mediated cleavage of cytoskeletal proteins.
R-RNO-6794361. Neurexins and neuroligins.
R-RNO-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Drebrin-like protein
Alternative name(s):
Actin-binding protein 1
Short name:
Abp1
SH3 domain-containing protein 7
Gene namesi
Name:DbnlImported
Synonyms:Sh3p7Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 14

Organism-specific databases

RGDi70941. Dbnl.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Endosome, Golgi apparatus, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000797951 – 436Drebrin-like proteinAdd BLAST436

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei26PhosphothreonineBy similarity1
Modified residuei160PhosphoserineBy similarity1
Modified residuei176N6-acetyllysineBy similarity1
Modified residuei277PhosphoserineBy similarity1
Modified residuei280PhosphoserineBy similarity1
Modified residuei283PhosphoserineBy similarity1
Modified residuei291PhosphoserineCombined sources1
Modified residuei296N6-acetyllysineBy similarity1
Modified residuei299PhosphothreonineCombined sources1
Modified residuei311PhosphoserineCombined sources1
Modified residuei340PhosphotyrosineBy similarity1
Modified residuei350PhosphotyrosineBy similarity1

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei367 – 368Cleavage; by caspase-3By similarity2

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9JHL4.
PRIDEiQ9JHL4.

PTM databases

iPTMnetiQ9JHL4.
PhosphoSitePlusiQ9JHL4.

Expressioni

Tissue specificityi

Detected in brain (at protein level). Widely expressed in brain with highest levels in hippocampus and cerebral cortex. Located primarily in dendrites and, in moderate amounts, in cell bodies. Isoform 1 and isoform 3 are the predominant isoforms in brain.3 Publications

Gene expression databases

BgeeiENSRNOG00000012378.
GenevisibleiQ9JHL4. RN.

Interactioni

Subunit structurei

Interacts with FGD1, MAP4K1 and PRAM1 (By similarity). Interacts with ANKRD54. Interacts with WASL and WIPF1 (By similarity). Interacts with SHANK2 and SHANK3. Interacts with both COBL and PACSIN1. Interacts with DNM1 and SYN1.By similarity5 Publications

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9JHL4. 2 interactors.
MINTiMINT-4568258.
STRINGi10116.ENSRNOP00000017375.

Structurei

3D structure databases

ProteinModelPortaliQ9JHL4.
SMRiQ9JHL4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 133ADF-HPROSITE-ProRule annotationAdd BLAST132
Domaini377 – 436SH3PROSITE-ProRule annotationAdd BLAST60

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili178 – 232Sequence analysisAdd BLAST55

Domaini

The SH3 domain mediates interaction with SHANK2, SHANK3 and PRAM1.

Sequence similaritiesi

Belongs to the ABP1 family.Curated

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiKOG3655. Eukaryota.
ENOG410XRVX. LUCA.
GeneTreeiENSGT00530000062953.
HOGENOMiHOG000008567.
HOVERGENiHBG051316.
InParanoidiQ9JHL4.
KOiK20520.
OMAiGYEGQTN.
OrthoDBiEOG091G05KP.
PhylomeDBiQ9JHL4.
TreeFamiTF318935.

Family and domain databases

Gene3Di3.40.20.10. 1 hit.
InterProiView protein in InterPro
IPR002108. ADF-H.
IPR029006. ADF-H/Gelsolin-like_dom.
IPR029923. Dbnl.
IPR001452. SH3_domain.
PANTHERiPTHR10829:SF30. PTHR10829:SF30. 1 hit.
PfamiView protein in Pfam
PF00241. Cofilin_ADF. 1 hit.
PF14604. SH3_9. 1 hit.
SMARTiView protein in SMART
SM00102. ADF. 1 hit.
SM00326. SH3. 1 hit.
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiView protein in PROSITE
PS51263. ADF_H. 1 hit.
PS50002. SH3. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q9JHL4-1) [UniParc]FASTAAdd to basket
Also known as: SH3P7r11 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVNLSRNGP ALQEAYVRVV TEKSPTDWAL FTYEGNSNDI RVAGTGEGGL
60 70 80 90 100
EELVEELNSG KVMYAFCRVK DPNSGLPKFV LINWTGEGVN DVRKGACANH
110 120 130 140 150
VSTMANFLKG AHVTINARAE EDVEPECIME KVAKASGANY SFHKESSCFQ
160 170 180 190 200
DVGPQAPVGS VYQKTNAVSE IKRVGKDNFW AKAEKEEENR RLEEKRRAEE
210 220 230 240 250
EKQRLEEERR ERELQEAARR EQRYQEQHRS AGPPSPSSRT GELEQEVVSR
260 270 280 290 300
SRQEWESAGQ QAPHPREIFK QKERAMSTTS VSSSQPGKLR SPFLQKQFTQ
310 320 330 340 350
PEASYGREPT SPVSRPAAGV CEELAPSTPP SAQTDDEPTY EVPSEQETLY
360 370 380 390 400
EEPPPVQQPG AGSGHIDNYM QSQDLSGQGL CARALYDYQA ADDTEISFDP
410 420 430
ENLITGIEVI DEGWWRGYGP DGHFGMFPAN YVELIE
Length:436
Mass (Da):48,613
Last modified:October 1, 2000 - v1
Checksum:i064CA1E6BAF84444
GO
Isoform 21 Publication (identifier: Q9JHL4-2) [UniParc]FASTAAdd to basket
Also known as: SH3PQ624187r21 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     236-238: Missing.

Show »
Length:433
Mass (Da):48,341
Checksum:i952FFF81E6A6EDAA
GO
Isoform 31 Publication (identifier: Q9JHL4-3) [UniParc]FASTAAdd to basket
Also known as: SH3P7r31 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     235-238: Missing.

Show »
Length:432
Mass (Da):48,254
Checksum:iE2D5E5C1D325B641
GO
Isoform 41 Publication (identifier: Q9JHL4-4) [UniParc]FASTAAdd to basket
Also known as: SH3P7r41 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     235-238: Missing.
     256-256: Missing.

Show »
Length:431
Mass (Da):48,125
Checksum:iC44CEABB3E41399E
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_050791235 – 238Missing in isoform 3 and isoform 4. 1 Publication4
Alternative sequenceiVSP_050792236 – 238Missing in isoform 2. 1 Publication3
Alternative sequenceiVSP_050793256Missing in isoform 4. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB038364 mRNA. Translation: BAA90866.1.
AB038365 mRNA. Translation: BAA90867.1.
AB039818 mRNA. Translation: BAA92708.1.
AB039819 mRNA. Translation: BAA92709.1.
AB009346 mRNA. Translation: BAA90819.1.
BC072483 mRNA. Translation: AAH72483.1.
RefSeqiNP_001264140.1. NM_001277211.1. [Q9JHL4-1]
NP_001264141.1. NM_001277212.1. [Q9JHL4-3]
NP_001264142.1. NM_001277213.1. [Q9JHL4-4]
NP_112642.1. NM_031352.2. [Q9JHL4-2]
UniGeneiRn.4115.

Genome annotation databases

EnsembliENSRNOT00000017375; ENSRNOP00000017375; ENSRNOG00000012378. [Q9JHL4-1]
GeneIDi83527.
KEGGirno:83527.
UCSCiRGD:70941. rat. [Q9JHL4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiDBNL_RAT
AccessioniPrimary (citable) accession number: Q9JHL4
Secondary accession number(s): Q9JM66, Q9JM67, Q9JM74
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: October 1, 2000
Last modified: September 27, 2017
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families