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Q9JHL1 (NHRF2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Na(+)/H(+) exchange regulatory cofactor NHE-RF2

Short name=NHERF-2
Alternative name(s):
NHE3 kinase A regulatory protein E3KARP
Octs2
SRY-interacting protein 1
Short name=SIP-1
Sodium-hydrogen exchanger regulatory factor 2
Solute carrier family 9 isoform A3 regulatory factor 2
Tyrosine kinase activator protein 1
Short name=TKA-1
Gene names
Name:Slc9a3r2
Synonyms:Nherf2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length337 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May also act as scaffold protein in the nucleus By similarity.

Subunit structure

Homodimer, and heterodimer with SLC9A3R1. Binds PDZK1. Interacts with SRY. Binds ADRB2, SLC9A3, P2RY1, P2YR2, RDX and LPAR2 By similarity. Interacts with MCC By similarity. Found in a complex with EZR, PODXL and SLC9A3R2 By similarity. Interacts (via the PDZ domains) with PODXL (via the C-terminal PDZ-binding motif DTHL); interaction is detected in glomerular epithelium cells By similarity. Interacts with SGK1 and KCNJ1/ROMK1 By similarity. Interacts (via the PDZ domains) with SLC26A6 By similarity. Ref.4 Ref.5

Subcellular location

Endomembrane system; Peripheral membrane protein By similarity. Nucleus By similarity. Apical cell membrane By similarity. Note: Localizes with EZR and PODXL at the apical cell membrane of glomerular epithelium cells and the sides of the food processes. Nuclear, in a punctate pattern By similarity.

Sequence similarities

Contains 2 PDZ (DHR) domains.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PdgfrbP056222EBI-538451,EBI-1554855
PTENP604842EBI-538451,EBI-696162From a different organism.
PtenO085863EBI-538451,EBI-1186266

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9JHL1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9JHL1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-111: Missing.
     112-138: LPPAHNPWEPKPDWACSGSLGSDTGQK → MARSRTSMLPASAPGAPPVNSQLGLTQ

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 337337Na(+)/H(+) exchange regulatory cofactor NHE-RF2
PRO_0000096806

Regions

Domain11 – 9181PDZ 1
Domain151 – 23181PDZ 2

Amino acid modifications

Modified residue1831Phosphoserine By similarity
Modified residue2541Phosphoserine By similarity

Natural variations

Alternative sequence1 – 111111Missing in isoform 2.
VSP_009379
Alternative sequence112 – 13827LPPAH…DTGQK → MARSRTSMLPASAPGAPPVN SQLGLTQ in isoform 2.
VSP_009380

Experimental info

Sequence conflict41P → L in BAC30366. Ref.2
Sequence conflict41P → L in BAC35980. Ref.2
Sequence conflict41P → L in BAC39282. Ref.2
Sequence conflict1431P → S in BAA97568. Ref.1
Sequence conflict1431P → S in BAA97569. Ref.1
Sequence conflict1431P → S in BAE41537. Ref.2
Sequence conflict2421V → I in BAC30366. Ref.2
Sequence conflict2421V → I in BAC35980. Ref.2
Sequence conflict2421V → I in BAC39282. Ref.2
Sequence conflict2421V → I in AAH65778. Ref.3
Sequence conflict3261D → A in BAB22185. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 29, 2007. Version 2.
Checksum: A28ED245DE453A5F

FASTA33737,403
        10         20         30         40         50         60 
MAAPESLRPR LCRLVRGEQG YGFHLHGEKG RRGQFIRRVE PGSPAEAAAL RAGDRLVEVN 

        70         80         90        100        110        120 
GVNVEGETHH QVVQRIKAVE GQTQLLVVDK ETDEELCRRQ LTCTEEMAHR GLPPAHNPWE 

       130        140        150        160        170        180 
PKPDWACSGS LGSDTGQKDV NGPPRELRPR LCHLRRGPQG YGFNLHSDKS RPGQYIRSVD 

       190        200        210        220        230        240 
PGSPASHSGL RAQDRLIEVN GQNVEGLRHA EVVARIKAQE DEARLLVVDP ETDEHFKRLR 

       250        260        270        280        290        300 
VVPTEEHVEG PLPSPVTNGT SPAQLNGGSV CSSRSDLPGS EKDNEDGSTW KRDPFQESGL 

       310        320        330 
HLSPTAAEAK EKARATRVNK RAPQMDWNRK REIFSNF 

« Hide

Isoform 2 [UniParc].

Checksum: 09162261FE6ABCD9
Show »

FASTA22624,861

References

« Hide 'large scale' references
[1]"cDNA cloning, genomic structure and sequence analysis of the mouse mSlc9a3r2/E3karp/Sip-1/Tka-1/Octs2 gene."
Sarker A.H., Akiyama K., Tsutsui K., Seki S.
Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1).
Strain: BALB/c.
Tissue: Blood and Embryo.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J and NOD.
Tissue: Embryonic heart, Kidney, Small intestine and Spinal cord.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Mammary gland.
[4]"PDZK1: I. a major scaffolder in brush borders of proximal tubular cells."
Gisler S.M., Pribanic S., Bacic D., Forrer P., Gantenbein A., Sabourin L.A., Tsuji A., Zhao Z.-S., Manser E., Biber J., Murer H.
Kidney Int. 64:1733-1745(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PDZK1.
[5]"NHERF2/SIP-1 interacts with mouse SRY via a different mechanism than human SRY."
Thevenet L., Albrecht K.H., Malki S., Berta P., Boizet-Bonhoure B., Poulat F.
J. Biol. Chem. 280:38625-38630(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SRY.
[6]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB026489 mRNA. Translation: BAA97568.1.
AB026490 Genomic DNA. Translation: BAA97569.1.
AK002557 mRNA. Translation: BAB22185.1.
AK039491 mRNA. Translation: BAC30366.1.
AK075813 mRNA. Translation: BAC35980.1.
AK084801 mRNA. Translation: BAC39282.1.
AK170057 mRNA. Translation: BAE41537.1.
BC065778 mRNA. Translation: AAH65778.1.
CCDSCCDS28488.1. [Q9JHL1-1]
CCDS28489.1. [Q9JHL1-2]
RefSeqNP_075542.2. NM_023055.2.
NP_075938.2. NM_023449.3.
UniGeneMm.21587.

3D structure databases

ProteinModelPortalQ9JHL1.
SMRQ9JHL1. Positions 9-91, 143-337.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid211159. 1 interaction.
IntActQ9JHL1. 7 interactions.
MINTMINT-152409.

PTM databases

PhosphoSiteQ9JHL1.

Proteomic databases

MaxQBQ9JHL1.
PaxDbQ9JHL1.
PRIDEQ9JHL1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000002572; ENSMUSP00000002572; ENSMUSG00000002504.
ENSMUST00000019684; ENSMUSP00000019684; ENSMUSG00000002504.
GeneID65962.
KEGGmmu:65962.
UCSCuc008axj.2. mouse. [Q9JHL1-2]
uc008axk.2. mouse. [Q9JHL1-1]

Organism-specific databases

CTD9351.
MGIMGI:1890662. Slc9a3r2.

Phylogenomic databases

eggNOGNOG319972.
GeneTreeENSGT00530000062999.
HOGENOMHOG000089940.
HOVERGENHBG052616.
InParanoidQ9JHL1.
KOK13358.
OrthoDBEOG7R56T6.
PhylomeDBQ9JHL1.
TreeFamTF350449.

Gene expression databases

ArrayExpressQ9JHL1.
BgeeQ9JHL1.
GenevestigatorQ9JHL1.

Family and domain databases

Gene3D2.30.42.10. 2 hits.
InterProIPR015098. EBP50_C-term.
IPR017300. NaH_exchngr_reg_CF_NHE-RF.
IPR001478. PDZ.
[Graphical view]
PfamPF09007. EBP50_C-term. 1 hit.
PF00595. PDZ. 2 hits.
[Graphical view]
PIRSFPIRSF037866. EBP50. 1 hit.
ProDomPD283022. EBP50_C-term. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00228. PDZ. 2 hits.
[Graphical view]
SUPFAMSSF50156. SSF50156. 2 hits.
PROSITEPS50106. PDZ. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio320388.
PROQ9JHL1.
SOURCESearch...

Entry information

Entry nameNHRF2_MOUSE
AccessionPrimary (citable) accession number: Q9JHL1
Secondary accession number(s): Q3TDR3 expand/collapse secondary AC list , Q6P074, Q8BGL9, Q8BW05, Q9DCR6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: May 29, 2007
Last modified: July 9, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot