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Q9JHL1

- NHRF2_MOUSE

UniProt

Q9JHL1 - NHRF2_MOUSE

Protein

Na(+)/H(+) exchange regulatory cofactor NHE-RF2

Gene

Slc9a3r2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 2 (29 May 2007)
      Previous versions | rss
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    Functioni

    Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May also act as scaffold protein in the nucleus By similarity.By similarity

    GO - Molecular functioni

    1. phosphatase binding Source: UniProtKB
    2. protein binding Source: UniProtKB

    GO - Biological processi

    1. negative regulation of phosphatidylinositol 3-kinase signaling Source: MGI

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Na(+)/H(+) exchange regulatory cofactor NHE-RF2
    Short name:
    NHERF-2
    Alternative name(s):
    NHE3 kinase A regulatory protein E3KARP
    Octs2
    SRY-interacting protein 1
    Short name:
    SIP-1
    Sodium-hydrogen exchanger regulatory factor 2
    Solute carrier family 9 isoform A3 regulatory factor 2
    Tyrosine kinase activator protein 1
    Short name:
    TKA-1
    Gene namesi
    Name:Slc9a3r2
    Synonyms:Nherf2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:1890662. Slc9a3r2.

    Subcellular locationi

    Endomembrane system By similarity; Peripheral membrane protein By similarity. Nucleus By similarity. Apical cell membrane By similarity
    Note: Localizes with EZR and PODXL at the apical cell membrane of glomerular epithelium cells and the sides of the food processes. Nuclear, in a punctate pattern By similarity.By similarity

    GO - Cellular componenti

    1. apical plasma membrane Source: MGI
    2. cytoplasm Source: MGI
    3. endomembrane system Source: UniProtKB-SubCell
    4. nucleus Source: MGI

    Keywords - Cellular componenti

    Cell membrane, Membrane, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 337337Na(+)/H(+) exchange regulatory cofactor NHE-RF2PRO_0000096806Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei183 – 1831PhosphoserineBy similarity
    Modified residuei254 – 2541PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ9JHL1.
    PaxDbiQ9JHL1.
    PRIDEiQ9JHL1.

    PTM databases

    PhosphoSiteiQ9JHL1.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9JHL1.
    BgeeiQ9JHL1.
    GenevestigatoriQ9JHL1.

    Interactioni

    Subunit structurei

    Homodimer, and heterodimer with SLC9A3R1. Binds PDZK1. Interacts with SRY. Binds ADRB2, SLC9A3, P2RY1, P2YR2, RDX and LPAR2 By similarity. Interacts with MCC By similarity. Found in a complex with EZR, PODXL and SLC9A3R2 By similarity. Interacts (via the PDZ domains) with PODXL (via the C-terminal PDZ-binding motif DTHL); interaction is detected in glomerular epithelium cells By similarity. Interacts with SGK1 and KCNJ1/ROMK1 By similarity. Interacts (via the PDZ domains) with SLC26A6 By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PdgfrbP056222EBI-538451,EBI-1554855
    PTENP604842EBI-538451,EBI-696162From a different organism.
    PtenO085863EBI-538451,EBI-1186266

    Protein-protein interaction databases

    BioGridi211159. 1 interaction.
    IntActiQ9JHL1. 7 interactions.
    MINTiMINT-152409.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9JHL1.
    SMRiQ9JHL1. Positions 9-91, 143-337.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini11 – 9181PDZ 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini151 – 23181PDZ 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 2 PDZ (DHR) domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG319972.
    GeneTreeiENSGT00530000062999.
    HOGENOMiHOG000089940.
    HOVERGENiHBG052616.
    InParanoidiQ9JHL1.
    KOiK13358.
    OrthoDBiEOG7R56T6.
    PhylomeDBiQ9JHL1.
    TreeFamiTF350449.

    Family and domain databases

    Gene3Di2.30.42.10. 2 hits.
    InterProiIPR015098. EBP50_C-term.
    IPR017300. NaH_exchngr_reg_CF_NHE-RF.
    IPR001478. PDZ.
    [Graphical view]
    PfamiPF09007. EBP50_C-term. 1 hit.
    PF00595. PDZ. 2 hits.
    [Graphical view]
    PIRSFiPIRSF037866. EBP50. 1 hit.
    ProDomiPD283022. EBP50_C-term. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00228. PDZ. 2 hits.
    [Graphical view]
    SUPFAMiSSF50156. SSF50156. 2 hits.
    PROSITEiPS50106. PDZ. 2 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9JHL1-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAAPESLRPR LCRLVRGEQG YGFHLHGEKG RRGQFIRRVE PGSPAEAAAL    50
    RAGDRLVEVN GVNVEGETHH QVVQRIKAVE GQTQLLVVDK ETDEELCRRQ 100
    LTCTEEMAHR GLPPAHNPWE PKPDWACSGS LGSDTGQKDV NGPPRELRPR 150
    LCHLRRGPQG YGFNLHSDKS RPGQYIRSVD PGSPASHSGL RAQDRLIEVN 200
    GQNVEGLRHA EVVARIKAQE DEARLLVVDP ETDEHFKRLR VVPTEEHVEG 250
    PLPSPVTNGT SPAQLNGGSV CSSRSDLPGS EKDNEDGSTW KRDPFQESGL 300
    HLSPTAAEAK EKARATRVNK RAPQMDWNRK REIFSNF 337
    Length:337
    Mass (Da):37,403
    Last modified:May 29, 2007 - v2
    Checksum:iA28ED245DE453A5F
    GO
    Isoform 2 (identifier: Q9JHL1-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-111: Missing.
         112-138: LPPAHNPWEPKPDWACSGSLGSDTGQK → MARSRTSMLPASAPGAPPVNSQLGLTQ

    Show »
    Length:226
    Mass (Da):24,861
    Checksum:i09162261FE6ABCD9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti4 – 41P → L in BAC30366. (PubMed:16141072)Curated
    Sequence conflicti4 – 41P → L in BAC35980. (PubMed:16141072)Curated
    Sequence conflicti4 – 41P → L in BAC39282. (PubMed:16141072)Curated
    Sequence conflicti143 – 1431P → S in BAA97568. 1 PublicationCurated
    Sequence conflicti143 – 1431P → S in BAA97569. 1 PublicationCurated
    Sequence conflicti143 – 1431P → S in BAE41537. (PubMed:16141072)Curated
    Sequence conflicti242 – 2421V → I in BAC30366. (PubMed:16141072)Curated
    Sequence conflicti242 – 2421V → I in BAC35980. (PubMed:16141072)Curated
    Sequence conflicti242 – 2421V → I in BAC39282. (PubMed:16141072)Curated
    Sequence conflicti242 – 2421V → I in AAH65778. (PubMed:15489334)Curated
    Sequence conflicti326 – 3261D → A in BAB22185. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 111111Missing in isoform 2. 2 PublicationsVSP_009379Add
    BLAST
    Alternative sequencei112 – 13827LPPAH…DTGQK → MARSRTSMLPASAPGAPPVN SQLGLTQ in isoform 2. 2 PublicationsVSP_009380Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB026489 mRNA. Translation: BAA97568.1.
    AB026490 Genomic DNA. Translation: BAA97569.1.
    AK002557 mRNA. Translation: BAB22185.1.
    AK039491 mRNA. Translation: BAC30366.1.
    AK075813 mRNA. Translation: BAC35980.1.
    AK084801 mRNA. Translation: BAC39282.1.
    AK170057 mRNA. Translation: BAE41537.1.
    BC065778 mRNA. Translation: AAH65778.1.
    CCDSiCCDS28488.1. [Q9JHL1-1]
    CCDS28489.1. [Q9JHL1-2]
    RefSeqiNP_075542.2. NM_023055.2.
    NP_075938.2. NM_023449.3.
    UniGeneiMm.21587.

    Genome annotation databases

    EnsembliENSMUST00000002572; ENSMUSP00000002572; ENSMUSG00000002504.
    ENSMUST00000019684; ENSMUSP00000019684; ENSMUSG00000002504.
    GeneIDi65962.
    KEGGimmu:65962.
    UCSCiuc008axj.2. mouse. [Q9JHL1-2]
    uc008axk.2. mouse. [Q9JHL1-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB026489 mRNA. Translation: BAA97568.1 .
    AB026490 Genomic DNA. Translation: BAA97569.1 .
    AK002557 mRNA. Translation: BAB22185.1 .
    AK039491 mRNA. Translation: BAC30366.1 .
    AK075813 mRNA. Translation: BAC35980.1 .
    AK084801 mRNA. Translation: BAC39282.1 .
    AK170057 mRNA. Translation: BAE41537.1 .
    BC065778 mRNA. Translation: AAH65778.1 .
    CCDSi CCDS28488.1. [Q9JHL1-1 ]
    CCDS28489.1. [Q9JHL1-2 ]
    RefSeqi NP_075542.2. NM_023055.2.
    NP_075938.2. NM_023449.3.
    UniGenei Mm.21587.

    3D structure databases

    ProteinModelPortali Q9JHL1.
    SMRi Q9JHL1. Positions 9-91, 143-337.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 211159. 1 interaction.
    IntActi Q9JHL1. 7 interactions.
    MINTi MINT-152409.

    PTM databases

    PhosphoSitei Q9JHL1.

    Proteomic databases

    MaxQBi Q9JHL1.
    PaxDbi Q9JHL1.
    PRIDEi Q9JHL1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000002572 ; ENSMUSP00000002572 ; ENSMUSG00000002504 .
    ENSMUST00000019684 ; ENSMUSP00000019684 ; ENSMUSG00000002504 .
    GeneIDi 65962.
    KEGGi mmu:65962.
    UCSCi uc008axj.2. mouse. [Q9JHL1-2 ]
    uc008axk.2. mouse. [Q9JHL1-1 ]

    Organism-specific databases

    CTDi 9351.
    MGIi MGI:1890662. Slc9a3r2.

    Phylogenomic databases

    eggNOGi NOG319972.
    GeneTreei ENSGT00530000062999.
    HOGENOMi HOG000089940.
    HOVERGENi HBG052616.
    InParanoidi Q9JHL1.
    KOi K13358.
    OrthoDBi EOG7R56T6.
    PhylomeDBi Q9JHL1.
    TreeFami TF350449.

    Miscellaneous databases

    NextBioi 320388.
    PROi Q9JHL1.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9JHL1.
    Bgeei Q9JHL1.
    Genevestigatori Q9JHL1.

    Family and domain databases

    Gene3Di 2.30.42.10. 2 hits.
    InterProi IPR015098. EBP50_C-term.
    IPR017300. NaH_exchngr_reg_CF_NHE-RF.
    IPR001478. PDZ.
    [Graphical view ]
    Pfami PF09007. EBP50_C-term. 1 hit.
    PF00595. PDZ. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF037866. EBP50. 1 hit.
    ProDomi PD283022. EBP50_C-term. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SMARTi SM00228. PDZ. 2 hits.
    [Graphical view ]
    SUPFAMi SSF50156. SSF50156. 2 hits.
    PROSITEi PS50106. PDZ. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA cloning, genomic structure and sequence analysis of the mouse mSlc9a3r2/E3karp/Sip-1/Tka-1/Octs2 gene."
      Sarker A.H., Akiyama K., Tsutsui K., Seki S.
      Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1).
      Strain: BALB/c.
      Tissue: Blood and Embryo.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6J and NOD.
      Tissue: Embryonic heart, Kidney, Small intestine and Spinal cord.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Mammary gland.
    4. Cited for: INTERACTION WITH PDZK1.
    5. "NHERF2/SIP-1 interacts with mouse SRY via a different mechanism than human SRY."
      Thevenet L., Albrecht K.H., Malki S., Berta P., Boizet-Bonhoure B., Poulat F.
      J. Biol. Chem. 280:38625-38630(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SRY.
    6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiNHRF2_MOUSE
    AccessioniPrimary (citable) accession number: Q9JHL1
    Secondary accession number(s): Q3TDR3
    , Q6P074, Q8BGL9, Q8BW05, Q9DCR6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 16, 2004
    Last sequence update: May 29, 2007
    Last modified: October 1, 2014
    This is version 114 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3