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Protein

Na(+)/H(+) exchange regulatory cofactor NHE-RF2

Gene

Slc9a3r2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May also act as scaffold protein in the nucleus (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • negative regulation of phosphatidylinositol 3-kinase signaling Source: MGI
  • sensory perception of sound Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Na(+)/H(+) exchange regulatory cofactor NHE-RF2
Short name:
NHERF-2
Alternative name(s):
NHE3 kinase A regulatory protein E3KARP
Octs2
SRY-interacting protein 1
Short name:
SIP-1
Sodium-hydrogen exchanger regulatory factor 2
Solute carrier family 9 isoform A3 regulatory factor 2
Tyrosine kinase activator protein 1
Short name:
TKA-1
Gene namesi
Name:Slc9a3r2
Synonyms:Nherf2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1890662. Slc9a3r2.

Subcellular locationi

GO - Cellular componenti

  • apical plasma membrane Source: MGI
  • cell-cell adherens junction Source: MGI
  • cytoplasm Source: MGI
  • endomembrane system Source: UniProtKB-SubCell
  • extracellular exosome Source: MGI
  • focal adhesion Source: MGI
  • nucleus Source: MGI
  • plasma membrane Source: MGI
  • stereocilium Source: MGI
  • stereocilium bundle Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000968061 – 337Na(+)/H(+) exchange regulatory cofactor NHE-RF2Add BLAST337

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei130PhosphoserineCombined sources1
Modified residuei183PhosphoserineBy similarity1
Modified residuei186PhosphoserineBy similarity1
Modified residuei254PhosphoserineBy similarity1
Modified residuei269PhosphoserineCombined sources1
Modified residuei280PhosphoserineCombined sources1
Modified residuei303PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9JHL1.
PeptideAtlasiQ9JHL1.
PRIDEiQ9JHL1.

PTM databases

iPTMnetiQ9JHL1.
PhosphoSitePlusiQ9JHL1.

Expressioni

Gene expression databases

BgeeiENSMUSG00000002504.

Interactioni

Subunit structurei

Homodimer, and heterodimer with SLC9A3R1. Binds PDZK1. Interacts with SRY. Binds ADRB2, SLC9A3, P2RY1, P2YR2, RDX and LPAR2 (By similarity). Interacts with MCC (By similarity). Found in a complex with EZR, PODXL and SLC9A3R2 (By similarity). Interacts (via the PDZ domains) with PODXL (via the C-terminal PDZ-binding motif DTHL); interaction is detected in glomerular epithelium cells (By similarity). Interacts with SGK1 and KCNJ1/ROMK1 (By similarity). Interacts (via the PDZ domains) with SLC26A6 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
PdgfrbP056222EBI-538451,EBI-1554855
PTENP604842EBI-538451,EBI-696162From a different organism.
PtenO085863EBI-538451,EBI-1186266

GO - Molecular functioni

Protein-protein interaction databases

BioGridi211159. 1 interactor.
IntActiQ9JHL1. 7 interactors.
MINTiMINT-152409.
STRINGi10090.ENSMUSP00000002572.

Structurei

3D structure databases

ProteinModelPortaliQ9JHL1.
SMRiQ9JHL1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 91PDZ 1PROSITE-ProRule annotationAdd BLAST81
Domaini151 – 231PDZ 2PROSITE-ProRule annotationAdd BLAST81

Sequence similaritiesi

Contains 2 PDZ (DHR) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410ITAC. Eukaryota.
ENOG4111V58. LUCA.
HOGENOMiHOG000089940.
HOVERGENiHBG052616.
InParanoidiQ9JHL1.
KOiK13358.
PhylomeDBiQ9JHL1.
TreeFamiTF350449.

Family and domain databases

Gene3Di2.30.42.10. 2 hits.
InterProiIPR015098. EBP50_C-term.
IPR017300. NHERF-1/NHERF-2.
IPR031197. NHERF-2.
IPR001478. PDZ.
[Graphical view]
PANTHERiPTHR14191:SF4. PTHR14191:SF4. 1 hit.
PfamiPF09007. EBP50_C. 2 hits.
PF00595. PDZ. 2 hits.
[Graphical view]
PIRSFiPIRSF037866. EBP50. 1 hit.
ProDomiPD283022. EBP50_C-term. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00228. PDZ. 2 hits.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 2 hits.
PROSITEiPS50106. PDZ. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9JHL1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAPESLRPR LCRLVRGEQG YGFHLHGEKG RRGQFIRRVE PGSPAEAAAL
60 70 80 90 100
RAGDRLVEVN GVNVEGETHH QVVQRIKAVE GQTQLLVVDK ETDEELCRRQ
110 120 130 140 150
LTCTEEMAHR GLPPAHNPWE PKPDWACSGS LGSDTGQKDV NGPPRELRPR
160 170 180 190 200
LCHLRRGPQG YGFNLHSDKS RPGQYIRSVD PGSPASHSGL RAQDRLIEVN
210 220 230 240 250
GQNVEGLRHA EVVARIKAQE DEARLLVVDP ETDEHFKRLR VVPTEEHVEG
260 270 280 290 300
PLPSPVTNGT SPAQLNGGSV CSSRSDLPGS EKDNEDGSTW KRDPFQESGL
310 320 330
HLSPTAAEAK EKARATRVNK RAPQMDWNRK REIFSNF
Length:337
Mass (Da):37,403
Last modified:May 29, 2007 - v2
Checksum:iA28ED245DE453A5F
GO
Isoform 2 (identifier: Q9JHL1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-111: Missing.
     112-138: LPPAHNPWEPKPDWACSGSLGSDTGQK → MARSRTSMLPASAPGAPPVNSQLGLTQ

Show »
Length:226
Mass (Da):24,861
Checksum:i09162261FE6ABCD9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti4P → L in BAC30366 (PubMed:16141072).Curated1
Sequence conflicti4P → L in BAC35980 (PubMed:16141072).Curated1
Sequence conflicti4P → L in BAC39282 (PubMed:16141072).Curated1
Sequence conflicti143P → S in BAA97568 (Ref. 1) Curated1
Sequence conflicti143P → S in BAA97569 (Ref. 1) Curated1
Sequence conflicti143P → S in BAE41537 (PubMed:16141072).Curated1
Sequence conflicti242V → I in BAC30366 (PubMed:16141072).Curated1
Sequence conflicti242V → I in BAC35980 (PubMed:16141072).Curated1
Sequence conflicti242V → I in BAC39282 (PubMed:16141072).Curated1
Sequence conflicti242V → I in AAH65778 (PubMed:15489334).Curated1
Sequence conflicti326D → A in BAB22185 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0093791 – 111Missing in isoform 2. 2 PublicationsAdd BLAST111
Alternative sequenceiVSP_009380112 – 138LPPAH…DTGQK → MARSRTSMLPASAPGAPPVN SQLGLTQ in isoform 2. 2 PublicationsAdd BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026489 mRNA. Translation: BAA97568.1.
AB026490 Genomic DNA. Translation: BAA97569.1.
AK002557 mRNA. Translation: BAB22185.1.
AK039491 mRNA. Translation: BAC30366.1.
AK075813 mRNA. Translation: BAC35980.1.
AK084801 mRNA. Translation: BAC39282.1.
AK170057 mRNA. Translation: BAE41537.1.
BC065778 mRNA. Translation: AAH65778.1.
CCDSiCCDS28488.1. [Q9JHL1-1]
CCDS28489.1. [Q9JHL1-2]
RefSeqiNP_075542.2. NM_023055.2.
NP_075938.2. NM_023449.3.
UniGeneiMm.21587.

Genome annotation databases

GeneIDi65962.
KEGGimmu:65962.
UCSCiuc008axj.2. mouse. [Q9JHL1-2]
uc008axk.2. mouse. [Q9JHL1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026489 mRNA. Translation: BAA97568.1.
AB026490 Genomic DNA. Translation: BAA97569.1.
AK002557 mRNA. Translation: BAB22185.1.
AK039491 mRNA. Translation: BAC30366.1.
AK075813 mRNA. Translation: BAC35980.1.
AK084801 mRNA. Translation: BAC39282.1.
AK170057 mRNA. Translation: BAE41537.1.
BC065778 mRNA. Translation: AAH65778.1.
CCDSiCCDS28488.1. [Q9JHL1-1]
CCDS28489.1. [Q9JHL1-2]
RefSeqiNP_075542.2. NM_023055.2.
NP_075938.2. NM_023449.3.
UniGeneiMm.21587.

3D structure databases

ProteinModelPortaliQ9JHL1.
SMRiQ9JHL1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211159. 1 interactor.
IntActiQ9JHL1. 7 interactors.
MINTiMINT-152409.
STRINGi10090.ENSMUSP00000002572.

PTM databases

iPTMnetiQ9JHL1.
PhosphoSitePlusiQ9JHL1.

Proteomic databases

PaxDbiQ9JHL1.
PeptideAtlasiQ9JHL1.
PRIDEiQ9JHL1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi65962.
KEGGimmu:65962.
UCSCiuc008axj.2. mouse. [Q9JHL1-2]
uc008axk.2. mouse. [Q9JHL1-1]

Organism-specific databases

CTDi9351.
MGIiMGI:1890662. Slc9a3r2.

Phylogenomic databases

eggNOGiENOG410ITAC. Eukaryota.
ENOG4111V58. LUCA.
HOGENOMiHOG000089940.
HOVERGENiHBG052616.
InParanoidiQ9JHL1.
KOiK13358.
PhylomeDBiQ9JHL1.
TreeFamiTF350449.

Miscellaneous databases

PROiQ9JHL1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000002504.

Family and domain databases

Gene3Di2.30.42.10. 2 hits.
InterProiIPR015098. EBP50_C-term.
IPR017300. NHERF-1/NHERF-2.
IPR031197. NHERF-2.
IPR001478. PDZ.
[Graphical view]
PANTHERiPTHR14191:SF4. PTHR14191:SF4. 1 hit.
PfamiPF09007. EBP50_C. 2 hits.
PF00595. PDZ. 2 hits.
[Graphical view]
PIRSFiPIRSF037866. EBP50. 1 hit.
ProDomiPD283022. EBP50_C-term. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00228. PDZ. 2 hits.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 2 hits.
PROSITEiPS50106. PDZ. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNHRF2_MOUSE
AccessioniPrimary (citable) accession number: Q9JHL1
Secondary accession number(s): Q3TDR3
, Q6P074, Q8BGL9, Q8BW05, Q9DCR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: May 29, 2007
Last modified: November 2, 2016
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.