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Q9JHL1

- NHRF2_MOUSE

UniProt

Q9JHL1 - NHRF2_MOUSE

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Protein

Na(+)/H(+) exchange regulatory cofactor NHE-RF2

Gene

Slc9a3r2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May also act as scaffold protein in the nucleus (By similarity).By similarity

GO - Molecular functioni

  1. phosphatase binding Source: UniProtKB

GO - Biological processi

  1. negative regulation of phosphatidylinositol 3-kinase signaling Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Na(+)/H(+) exchange regulatory cofactor NHE-RF2
Short name:
NHERF-2
Alternative name(s):
NHE3 kinase A regulatory protein E3KARP
Octs2
SRY-interacting protein 1
Short name:
SIP-1
Sodium-hydrogen exchanger regulatory factor 2
Solute carrier family 9 isoform A3 regulatory factor 2
Tyrosine kinase activator protein 1
Short name:
TKA-1
Gene namesi
Name:Slc9a3r2
Synonyms:Nherf2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:1890662. Slc9a3r2.

Subcellular locationi

Endomembrane system By similarity; Peripheral membrane protein By similarity. Nucleus By similarity. Apical cell membrane By similarity
Note: Localizes with EZR and PODXL at the apical cell membrane of glomerular epithelium cells and the sides of the food processes. Nuclear, in a punctate pattern (By similarity).By similarity

GO - Cellular componenti

  1. apical plasma membrane Source: MGI
  2. cytoplasm Source: MGI
  3. extracellular vesicular exosome Source: Ensembl
  4. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 337337Na(+)/H(+) exchange regulatory cofactor NHE-RF2PRO_0000096806Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei183 – 1831PhosphoserineBy similarity
Modified residuei254 – 2541PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9JHL1.
PaxDbiQ9JHL1.
PRIDEiQ9JHL1.

PTM databases

PhosphoSiteiQ9JHL1.

Expressioni

Gene expression databases

BgeeiQ9JHL1.
ExpressionAtlasiQ9JHL1. baseline and differential.
GenevestigatoriQ9JHL1.

Interactioni

Subunit structurei

Homodimer, and heterodimer with SLC9A3R1. Binds PDZK1. Interacts with SRY. Binds ADRB2, SLC9A3, P2RY1, P2YR2, RDX and LPAR2 (By similarity). Interacts with MCC (By similarity). Found in a complex with EZR, PODXL and SLC9A3R2 (By similarity). Interacts (via the PDZ domains) with PODXL (via the C-terminal PDZ-binding motif DTHL); interaction is detected in glomerular epithelium cells (By similarity). Interacts with SGK1 and KCNJ1/ROMK1 (By similarity). Interacts (via the PDZ domains) with SLC26A6 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
PdgfrbP056222EBI-538451,EBI-1554855
PTENP604842EBI-538451,EBI-696162From a different organism.
PtenO085863EBI-538451,EBI-1186266

Protein-protein interaction databases

BioGridi211159. 1 interaction.
IntActiQ9JHL1. 7 interactions.
MINTiMINT-152409.

Structurei

3D structure databases

ProteinModelPortaliQ9JHL1.
SMRiQ9JHL1. Positions 9-90, 143-337.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 9181PDZ 1PROSITE-ProRule annotationAdd
BLAST
Domaini151 – 23181PDZ 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 PDZ (DHR) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG319972.
GeneTreeiENSGT00530000062999.
HOGENOMiHOG000089940.
HOVERGENiHBG052616.
InParanoidiQ9JHL1.
KOiK13358.
OrthoDBiEOG7R56T6.
PhylomeDBiQ9JHL1.
TreeFamiTF350449.

Family and domain databases

Gene3Di2.30.42.10. 2 hits.
InterProiIPR015098. EBP50_C-term.
IPR017300. NaH_exchngr_reg_CF_NHE-RF.
IPR001478. PDZ.
[Graphical view]
PfamiPF09007. EBP50_C-term. 1 hit.
PF00595. PDZ. 2 hits.
[Graphical view]
PIRSFiPIRSF037866. EBP50. 1 hit.
ProDomiPD283022. EBP50_C-term. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00228. PDZ. 2 hits.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 2 hits.
PROSITEiPS50106. PDZ. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9JHL1-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAPESLRPR LCRLVRGEQG YGFHLHGEKG RRGQFIRRVE PGSPAEAAAL
60 70 80 90 100
RAGDRLVEVN GVNVEGETHH QVVQRIKAVE GQTQLLVVDK ETDEELCRRQ
110 120 130 140 150
LTCTEEMAHR GLPPAHNPWE PKPDWACSGS LGSDTGQKDV NGPPRELRPR
160 170 180 190 200
LCHLRRGPQG YGFNLHSDKS RPGQYIRSVD PGSPASHSGL RAQDRLIEVN
210 220 230 240 250
GQNVEGLRHA EVVARIKAQE DEARLLVVDP ETDEHFKRLR VVPTEEHVEG
260 270 280 290 300
PLPSPVTNGT SPAQLNGGSV CSSRSDLPGS EKDNEDGSTW KRDPFQESGL
310 320 330
HLSPTAAEAK EKARATRVNK RAPQMDWNRK REIFSNF
Length:337
Mass (Da):37,403
Last modified:May 29, 2007 - v2
Checksum:iA28ED245DE453A5F
GO
Isoform 2 (identifier: Q9JHL1-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-111: Missing.
     112-138: LPPAHNPWEPKPDWACSGSLGSDTGQK → MARSRTSMLPASAPGAPPVNSQLGLTQ

Show »
Length:226
Mass (Da):24,861
Checksum:i09162261FE6ABCD9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 41P → L in BAC30366. (PubMed:16141072)Curated
Sequence conflicti4 – 41P → L in BAC35980. (PubMed:16141072)Curated
Sequence conflicti4 – 41P → L in BAC39282. (PubMed:16141072)Curated
Sequence conflicti143 – 1431P → S in BAA97568. 1 PublicationCurated
Sequence conflicti143 – 1431P → S in BAA97569. 1 PublicationCurated
Sequence conflicti143 – 1431P → S in BAE41537. (PubMed:16141072)Curated
Sequence conflicti242 – 2421V → I in BAC30366. (PubMed:16141072)Curated
Sequence conflicti242 – 2421V → I in BAC35980. (PubMed:16141072)Curated
Sequence conflicti242 – 2421V → I in BAC39282. (PubMed:16141072)Curated
Sequence conflicti242 – 2421V → I in AAH65778. (PubMed:15489334)Curated
Sequence conflicti326 – 3261D → A in BAB22185. (PubMed:16141072)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 111111Missing in isoform 2. 2 PublicationsVSP_009379Add
BLAST
Alternative sequencei112 – 13827LPPAH…DTGQK → MARSRTSMLPASAPGAPPVN SQLGLTQ in isoform 2. 2 PublicationsVSP_009380Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB026489 mRNA. Translation: BAA97568.1.
AB026490 Genomic DNA. Translation: BAA97569.1.
AK002557 mRNA. Translation: BAB22185.1.
AK039491 mRNA. Translation: BAC30366.1.
AK075813 mRNA. Translation: BAC35980.1.
AK084801 mRNA. Translation: BAC39282.1.
AK170057 mRNA. Translation: BAE41537.1.
BC065778 mRNA. Translation: AAH65778.1.
CCDSiCCDS28488.1. [Q9JHL1-1]
CCDS28489.1. [Q9JHL1-2]
RefSeqiNP_075542.2. NM_023055.2.
NP_075938.2. NM_023449.3.
UniGeneiMm.21587.

Genome annotation databases

EnsembliENSMUST00000002572; ENSMUSP00000002572; ENSMUSG00000002504.
ENSMUST00000019684; ENSMUSP00000019684; ENSMUSG00000002504.
GeneIDi65962.
KEGGimmu:65962.
UCSCiuc008axj.2. mouse. [Q9JHL1-2]
uc008axk.2. mouse. [Q9JHL1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB026489 mRNA. Translation: BAA97568.1 .
AB026490 Genomic DNA. Translation: BAA97569.1 .
AK002557 mRNA. Translation: BAB22185.1 .
AK039491 mRNA. Translation: BAC30366.1 .
AK075813 mRNA. Translation: BAC35980.1 .
AK084801 mRNA. Translation: BAC39282.1 .
AK170057 mRNA. Translation: BAE41537.1 .
BC065778 mRNA. Translation: AAH65778.1 .
CCDSi CCDS28488.1. [Q9JHL1-1 ]
CCDS28489.1. [Q9JHL1-2 ]
RefSeqi NP_075542.2. NM_023055.2.
NP_075938.2. NM_023449.3.
UniGenei Mm.21587.

3D structure databases

ProteinModelPortali Q9JHL1.
SMRi Q9JHL1. Positions 9-90, 143-337.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 211159. 1 interaction.
IntActi Q9JHL1. 7 interactions.
MINTi MINT-152409.

PTM databases

PhosphoSitei Q9JHL1.

Proteomic databases

MaxQBi Q9JHL1.
PaxDbi Q9JHL1.
PRIDEi Q9JHL1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000002572 ; ENSMUSP00000002572 ; ENSMUSG00000002504 .
ENSMUST00000019684 ; ENSMUSP00000019684 ; ENSMUSG00000002504 .
GeneIDi 65962.
KEGGi mmu:65962.
UCSCi uc008axj.2. mouse. [Q9JHL1-2 ]
uc008axk.2. mouse. [Q9JHL1-1 ]

Organism-specific databases

CTDi 9351.
MGIi MGI:1890662. Slc9a3r2.

Phylogenomic databases

eggNOGi NOG319972.
GeneTreei ENSGT00530000062999.
HOGENOMi HOG000089940.
HOVERGENi HBG052616.
InParanoidi Q9JHL1.
KOi K13358.
OrthoDBi EOG7R56T6.
PhylomeDBi Q9JHL1.
TreeFami TF350449.

Miscellaneous databases

NextBioi 320388.
PROi Q9JHL1.
SOURCEi Search...

Gene expression databases

Bgeei Q9JHL1.
ExpressionAtlasi Q9JHL1. baseline and differential.
Genevestigatori Q9JHL1.

Family and domain databases

Gene3Di 2.30.42.10. 2 hits.
InterProi IPR015098. EBP50_C-term.
IPR017300. NaH_exchngr_reg_CF_NHE-RF.
IPR001478. PDZ.
[Graphical view ]
Pfami PF09007. EBP50_C-term. 1 hit.
PF00595. PDZ. 2 hits.
[Graphical view ]
PIRSFi PIRSF037866. EBP50. 1 hit.
ProDomi PD283022. EBP50_C-term. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SMARTi SM00228. PDZ. 2 hits.
[Graphical view ]
SUPFAMi SSF50156. SSF50156. 2 hits.
PROSITEi PS50106. PDZ. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA cloning, genomic structure and sequence analysis of the mouse mSlc9a3r2/E3karp/Sip-1/Tka-1/Octs2 gene."
    Sarker A.H., Akiyama K., Tsutsui K., Seki S.
    Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1).
    Strain: BALB/c.
    Tissue: Blood and Embryo.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J and NOD.
    Tissue: Embryonic heart, Kidney, Small intestine and Spinal cord.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Mammary gland.
  4. Cited for: INTERACTION WITH PDZK1.
  5. "NHERF2/SIP-1 interacts with mouse SRY via a different mechanism than human SRY."
    Thevenet L., Albrecht K.H., Malki S., Berta P., Boizet-Bonhoure B., Poulat F.
    J. Biol. Chem. 280:38625-38630(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SRY.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiNHRF2_MOUSE
AccessioniPrimary (citable) accession number: Q9JHL1
Secondary accession number(s): Q3TDR3
, Q6P074, Q8BGL9, Q8BW05, Q9DCR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: May 29, 2007
Last modified: October 29, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3