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Protein

Pleckstrin

Gene

Plek

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Major protein kinase C substrate of platelets.

GO - Molecular functioni

GO - Biological processi

  • actin cytoskeleton reorganization Source: MGI
  • cell projection organization Source: MGI
  • cortical actin cytoskeleton organization Source: MGI
  • hematopoietic progenitor cell differentiation Source: Ensembl
  • integrin-mediated signaling pathway Source: MGI
  • negative regulation of G-protein coupled receptor protein signaling pathway Source: MGI
  • negative regulation of inositol phosphate biosynthetic process Source: MGI
  • phosphatidylinositol metabolic process Source: MGI
  • phospholipase C-inhibiting G-protein coupled receptor signaling pathway Source: MGI
  • platelet aggregation Source: BHF-UCL
  • positive regulation of actin filament bundle assembly Source: MGI
  • positive regulation of actin filament depolymerization Source: BHF-UCL
  • positive regulation of inositol-polyphosphate 5-phosphatase activity Source: MGI
  • positive regulation of integrin activation Source: BHF-UCL
  • positive regulation of platelet activation Source: BHF-UCL
  • protein kinase C signaling Source: BHF-UCL
  • protein secretion by platelet Source: BHF-UCL
  • regulation of cell diameter Source: MGI
  • ruffle organization Source: MGI
  • thrombin receptor signaling pathway Source: BHF-UCL
  • vesicle docking involved in exocytosis Source: BHF-UCL
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-114608. Platelet degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Pleckstrin
Gene namesi
Name:Plek
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1860485. Plek.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 350350PleckstrinPRO_0000053860Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei64 – 641N6-acetyllysineBy similarity
Modified residuei113 – 1131PhosphoserineBy similarity
Modified residuei117 – 1171PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9JHK5.
MaxQBiQ9JHK5.
PaxDbiQ9JHK5.
PRIDEiQ9JHK5.

PTM databases

iPTMnetiQ9JHK5.
PhosphoSiteiQ9JHK5.

Expressioni

Gene expression databases

BgeeiQ9JHK5.
CleanExiMM_PLEK.
ExpressionAtlasiQ9JHK5. baseline and differential.
GenevisibleiQ9JHK5. MM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi207832. 2 interactions.
STRINGi10090.ENSMUSP00000099945.

Structurei

Secondary structure

1
350
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi126 – 1349Combined sources
Turni136 – 1383Combined sources
Beta strandi143 – 1497Combined sources
Beta strandi151 – 1555Combined sources
Helixi158 – 16811Combined sources
Beta strandi170 – 1734Combined sources
Helixi174 – 18714Combined sources
Beta strandi189 – 1924Combined sources
Beta strandi194 – 1963Combined sources
Helixi197 – 2037Combined sources
Beta strandi204 – 2063Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1UHWNMR-A126-221[»]
ProteinModelPortaliQ9JHK5.
SMRiQ9JHK5. Positions 1-105, 128-223, 234-350.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9JHK5.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 10198PH 1PROSITE-ProRule annotationAdd
BLAST
Domaini136 – 22186DEPPROSITE-ProRule annotationAdd
BLAST
Domaini244 – 347104PH 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 DEP domain.PROSITE-ProRule annotation
Contains 2 PH domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IFJ7. Eukaryota.
ENOG410ZY9B. LUCA.
GeneTreeiENSGT00390000006543.
HOGENOMiHOG000294078.
HOVERGENiHBG001361.
InParanoidiQ9JHK5.
KOiK19993.
OMAiFCEGYSS.
OrthoDBiEOG74XS6R.
PhylomeDBiQ9JHK5.
TreeFamiTF332246.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.30.29.30. 2 hits.
InterProiIPR000591. DEP_dom.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00610. DEP. 1 hit.
PF00169. PH. 2 hits.
[Graphical view]
SMARTiSM00049. DEP. 1 hit.
SM00233. PH. 2 hits.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF50729. SSF50729. 2 hits.
PROSITEiPS50186. DEP. 1 hit.
PS50003. PH_DOMAIN. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JHK5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPKRIREGY LVKKGSVFNT WKPMWVVLLE DGIEFYKKKS DNSPKGMIPL
60 70 80 90 100
KGSTLTSPCQ DFGKRMFVLK ITTTKQQDHF FQAAFLEERD AWVRDIKKAI
110 120 130 140 150
KCIEGGQKFA RKSTRRSIRL PETIDLGALY LSMKDPEKGI KELNLEKDKK
160 170 180 190 200
VFNHCLTGSG VIDWLVSNKL VRNRQEGLMI SASLLSEGYL QPAGDLSKNA
210 220 230 240 250
ADGIAENPFL DSPDAFYYFP DSGFFCEENS SDDDVILREE FRGVIIKQGC
260 270 280 290 300
LLKQGHRRKN WKVRKFILRE DPAYLHYYDP AGGEDPLGAV HLRGCVVTSV
310 320 330 340 350
ESSHDVKKSD EENLFEIITA DEVHYYLQAA TSKERTEWIK AIQVASRTGK
Length:350
Mass (Da):39,901
Last modified:October 1, 2000 - v1
Checksum:i348F3CB469B7CC53
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti120 – 1201L → Q in AAG29513 (Ref. 3) Curated
Sequence conflicti225 – 2251F → L in AAG29513 (Ref. 3) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF181829 mRNA. Translation: AAF75830.1.
AF073294 mRNA. Translation: AAF72039.1.
AF303745 mRNA. Translation: AAG29513.1.
BC066185 mRNA. Translation: AAH66185.1.
CCDSiCCDS24446.1.
RefSeqiNP_062422.1. NM_019549.2.
UniGeneiMm.479819.
Mm.98232.

Genome annotation databases

EnsembliENSMUST00000102881; ENSMUSP00000099945; ENSMUSG00000020120.
GeneIDi56193.
KEGGimmu:56193.
UCSCiuc007ibv.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF181829 mRNA. Translation: AAF75830.1.
AF073294 mRNA. Translation: AAF72039.1.
AF303745 mRNA. Translation: AAG29513.1.
BC066185 mRNA. Translation: AAH66185.1.
CCDSiCCDS24446.1.
RefSeqiNP_062422.1. NM_019549.2.
UniGeneiMm.479819.
Mm.98232.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1UHWNMR-A126-221[»]
ProteinModelPortaliQ9JHK5.
SMRiQ9JHK5. Positions 1-105, 128-223, 234-350.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207832. 2 interactions.
STRINGi10090.ENSMUSP00000099945.

PTM databases

iPTMnetiQ9JHK5.
PhosphoSiteiQ9JHK5.

Proteomic databases

EPDiQ9JHK5.
MaxQBiQ9JHK5.
PaxDbiQ9JHK5.
PRIDEiQ9JHK5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102881; ENSMUSP00000099945; ENSMUSG00000020120.
GeneIDi56193.
KEGGimmu:56193.
UCSCiuc007ibv.2. mouse.

Organism-specific databases

CTDi5341.
MGIiMGI:1860485. Plek.

Phylogenomic databases

eggNOGiENOG410IFJ7. Eukaryota.
ENOG410ZY9B. LUCA.
GeneTreeiENSGT00390000006543.
HOGENOMiHOG000294078.
HOVERGENiHBG001361.
InParanoidiQ9JHK5.
KOiK19993.
OMAiFCEGYSS.
OrthoDBiEOG74XS6R.
PhylomeDBiQ9JHK5.
TreeFamiTF332246.

Enzyme and pathway databases

ReactomeiR-MMU-114608. Platelet degranulation.

Miscellaneous databases

EvolutionaryTraceiQ9JHK5.
PROiQ9JHK5.
SOURCEiSearch...

Gene expression databases

BgeeiQ9JHK5.
CleanExiMM_PLEK.
ExpressionAtlasiQ9JHK5. baseline and differential.
GenevisibleiQ9JHK5. MM.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.30.29.30. 2 hits.
InterProiIPR000591. DEP_dom.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00610. DEP. 1 hit.
PF00169. PH. 2 hits.
[Graphical view]
SMARTiSM00049. DEP. 1 hit.
SM00233. PH. 2 hits.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF50729. SSF50729. 2 hits.
PROSITEiPS50186. DEP. 1 hit.
PS50003. PH_DOMAIN. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA cloning and mapping of mouse pleckstrin (Plek), a gene upregulated in transformation-resistant cells."
    Cmarik J.L., Hegamyer G., Gerrard B., Dean M., Colburn N.H.
    Genomics 66:204-212(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/cJ.
  2. "Involvement of pleckstrin in B cell differentiation and activation."
    Zhang Y., Wu G., Paige C.J.
    Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Mouse pleckstrin 1 is induced in mast cells after IgE cross-linking."
    Ahn H.-J., Cho J.-J.
    Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-117, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Heart, Lung, Spleen and Testis.
  6. "Solution structure of the DEP domain of mouse pleckstrin."
    RIKEN structural genomics initiative (RSGI)
    Submitted (JAN-2004) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 126-223.

Entry informationi

Entry nameiPLEK_MOUSE
AccessioniPrimary (citable) accession number: Q9JHK5
Secondary accession number(s): Q9ERI9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2002
Last sequence update: October 1, 2000
Last modified: June 8, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.