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Protein

Pleckstrin

Gene

Plek

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Major protein kinase C substrate of platelets.

GO - Molecular functioni

GO - Biological processi

  • actin cytoskeleton reorganization Source: MGI
  • cell projection organization Source: MGI
  • cortical actin cytoskeleton organization Source: MGI
  • hematopoietic progenitor cell differentiation Source: Ensembl
  • integrin-mediated signaling pathway Source: MGI
  • negative regulation of G-protein coupled receptor protein signaling pathway Source: MGI
  • negative regulation of inositol phosphate biosynthetic process Source: MGI
  • phosphatidylinositol metabolic process Source: MGI
  • phospholipase C-inhibiting G-protein coupled receptor signaling pathway Source: MGI
  • platelet aggregation Source: BHF-UCL
  • positive regulation of actin filament bundle assembly Source: MGI
  • positive regulation of actin filament depolymerization Source: BHF-UCL
  • positive regulation of inositol-polyphosphate 5-phosphatase activity Source: MGI
  • positive regulation of integrin activation Source: BHF-UCL
  • positive regulation of platelet activation Source: BHF-UCL
  • protein kinase C signaling Source: BHF-UCL
  • protein secretion by platelet Source: BHF-UCL
  • regulation of cell diameter Source: MGI
  • ruffle organization Source: MGI
  • thrombin-activated receptor signaling pathway Source: BHF-UCL
  • vesicle docking involved in exocytosis Source: BHF-UCL
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-114608. Platelet degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Pleckstrin
Gene namesi
Name:Plek
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1860485. Plek.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000538601 – 350PleckstrinAdd BLAST350

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei64N6-acetyllysineBy similarity1
Modified residuei113PhosphoserineBy similarity1
Modified residuei117PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9JHK5.
MaxQBiQ9JHK5.
PaxDbiQ9JHK5.
PRIDEiQ9JHK5.

PTM databases

iPTMnetiQ9JHK5.
PhosphoSitePlusiQ9JHK5.

Expressioni

Gene expression databases

BgeeiENSMUSG00000020120.
CleanExiMM_PLEK.
ExpressionAtlasiQ9JHK5. baseline and differential.
GenevisibleiQ9JHK5. MM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi207832. 2 interactors.
STRINGi10090.ENSMUSP00000099945.

Structurei

Secondary structure

1350
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi126 – 134Combined sources9
Turni136 – 138Combined sources3
Beta strandi143 – 149Combined sources7
Beta strandi151 – 155Combined sources5
Helixi158 – 168Combined sources11
Beta strandi170 – 173Combined sources4
Helixi174 – 187Combined sources14
Beta strandi189 – 192Combined sources4
Beta strandi194 – 196Combined sources3
Helixi197 – 203Combined sources7
Beta strandi204 – 206Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UHWNMR-A126-221[»]
ProteinModelPortaliQ9JHK5.
SMRiQ9JHK5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9JHK5.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 101PH 1PROSITE-ProRule annotationAdd BLAST98
Domaini136 – 221DEPPROSITE-ProRule annotationAdd BLAST86
Domaini244 – 347PH 2PROSITE-ProRule annotationAdd BLAST104

Sequence similaritiesi

Contains 1 DEP domain.PROSITE-ProRule annotation
Contains 2 PH domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IFJ7. Eukaryota.
ENOG410ZY9B. LUCA.
GeneTreeiENSGT00390000006543.
HOGENOMiHOG000294078.
HOVERGENiHBG001361.
InParanoidiQ9JHK5.
KOiK19993.
OMAiFCEGYSS.
OrthoDBiEOG091G09EY.
PhylomeDBiQ9JHK5.
TreeFamiTF332246.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.30.29.30. 2 hits.
InterProiIPR000591. DEP_dom.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00610. DEP. 1 hit.
PF00169. PH. 2 hits.
[Graphical view]
SMARTiSM00049. DEP. 1 hit.
SM00233. PH. 2 hits.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF50729. SSF50729. 2 hits.
PROSITEiPS50186. DEP. 1 hit.
PS50003. PH_DOMAIN. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JHK5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPKRIREGY LVKKGSVFNT WKPMWVVLLE DGIEFYKKKS DNSPKGMIPL
60 70 80 90 100
KGSTLTSPCQ DFGKRMFVLK ITTTKQQDHF FQAAFLEERD AWVRDIKKAI
110 120 130 140 150
KCIEGGQKFA RKSTRRSIRL PETIDLGALY LSMKDPEKGI KELNLEKDKK
160 170 180 190 200
VFNHCLTGSG VIDWLVSNKL VRNRQEGLMI SASLLSEGYL QPAGDLSKNA
210 220 230 240 250
ADGIAENPFL DSPDAFYYFP DSGFFCEENS SDDDVILREE FRGVIIKQGC
260 270 280 290 300
LLKQGHRRKN WKVRKFILRE DPAYLHYYDP AGGEDPLGAV HLRGCVVTSV
310 320 330 340 350
ESSHDVKKSD EENLFEIITA DEVHYYLQAA TSKERTEWIK AIQVASRTGK
Length:350
Mass (Da):39,901
Last modified:October 1, 2000 - v1
Checksum:i348F3CB469B7CC53
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti120L → Q in AAG29513 (Ref. 3) Curated1
Sequence conflicti225F → L in AAG29513 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF181829 mRNA. Translation: AAF75830.1.
AF073294 mRNA. Translation: AAF72039.1.
AF303745 mRNA. Translation: AAG29513.1.
BC066185 mRNA. Translation: AAH66185.1.
CCDSiCCDS24446.1.
RefSeqiNP_062422.1. NM_019549.2.
UniGeneiMm.479819.
Mm.98232.

Genome annotation databases

EnsembliENSMUST00000102881; ENSMUSP00000099945; ENSMUSG00000020120.
GeneIDi56193.
KEGGimmu:56193.
UCSCiuc007ibv.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF181829 mRNA. Translation: AAF75830.1.
AF073294 mRNA. Translation: AAF72039.1.
AF303745 mRNA. Translation: AAG29513.1.
BC066185 mRNA. Translation: AAH66185.1.
CCDSiCCDS24446.1.
RefSeqiNP_062422.1. NM_019549.2.
UniGeneiMm.479819.
Mm.98232.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UHWNMR-A126-221[»]
ProteinModelPortaliQ9JHK5.
SMRiQ9JHK5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207832. 2 interactors.
STRINGi10090.ENSMUSP00000099945.

PTM databases

iPTMnetiQ9JHK5.
PhosphoSitePlusiQ9JHK5.

Proteomic databases

EPDiQ9JHK5.
MaxQBiQ9JHK5.
PaxDbiQ9JHK5.
PRIDEiQ9JHK5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102881; ENSMUSP00000099945; ENSMUSG00000020120.
GeneIDi56193.
KEGGimmu:56193.
UCSCiuc007ibv.2. mouse.

Organism-specific databases

CTDi5341.
MGIiMGI:1860485. Plek.

Phylogenomic databases

eggNOGiENOG410IFJ7. Eukaryota.
ENOG410ZY9B. LUCA.
GeneTreeiENSGT00390000006543.
HOGENOMiHOG000294078.
HOVERGENiHBG001361.
InParanoidiQ9JHK5.
KOiK19993.
OMAiFCEGYSS.
OrthoDBiEOG091G09EY.
PhylomeDBiQ9JHK5.
TreeFamiTF332246.

Enzyme and pathway databases

ReactomeiR-MMU-114608. Platelet degranulation.

Miscellaneous databases

EvolutionaryTraceiQ9JHK5.
PROiQ9JHK5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020120.
CleanExiMM_PLEK.
ExpressionAtlasiQ9JHK5. baseline and differential.
GenevisibleiQ9JHK5. MM.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.30.29.30. 2 hits.
InterProiIPR000591. DEP_dom.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00610. DEP. 1 hit.
PF00169. PH. 2 hits.
[Graphical view]
SMARTiSM00049. DEP. 1 hit.
SM00233. PH. 2 hits.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF50729. SSF50729. 2 hits.
PROSITEiPS50186. DEP. 1 hit.
PS50003. PH_DOMAIN. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPLEK_MOUSE
AccessioniPrimary (citable) accession number: Q9JHK5
Secondary accession number(s): Q9ERI9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2002
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.