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Q9JHK4

- PGTA_MOUSE

UniProt

Q9JHK4 - PGTA_MOUSE

Protein

Geranylgeranyl transferase type-2 subunit alpha

Gene

Rabggta

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Catalyzes the transfer of a geranylgeranyl moiety from geranylgeranyl diphosphate to both cysteines of Rab proteins with the C-terminal sequence -XXCC, -XCXC and -CCXX, such as RAB1A, RAB3A, RAB5A and RAB7A.1 Publication

    Catalytic activityi

    Geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate.

    Enzyme regulationi

    The enzymatic reaction requires the aid of a Rab escort protein (also called component A), such as CHM.By similarity

    GO - Molecular functioni

    1. Rab geranylgeranyltransferase activity Source: MGI
    2. Rab GTPase binding Source: UniProtKB
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. protein geranylgeranylation Source: MGI

    Keywords - Molecular functioni

    Prenyltransferase, Transferase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Geranylgeranyl transferase type-2 subunit alpha (EC:2.5.1.60)
    Alternative name(s):
    Geranylgeranyl transferase type II subunit alpha
    Rab geranyl-geranyltransferase subunit alpha
    Short name:
    Rab GG transferase alpha
    Short name:
    Rab GGTase alpha
    Rab geranylgeranyltransferase subunit alpha
    Gene namesi
    Name:Rabggta
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 14

    Organism-specific databases

    MGIiMGI:1860443. Rabggta.

    Subcellular locationi

    GO - Cellular componenti

    1. Rab-protein geranylgeranyltransferase complex Source: UniProtKB

    Pathology & Biotechi

    Involvement in diseasei

    Defects in Rabggta are the cause of the gunmetal (gm) phenotype. Mice homozygous for gm have prolonged bleeding, thrombocytopenia and reduced platelet alpha- and delta-granule contents.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 567567Geranylgeranyl transferase type-2 subunit alphaPRO_0000119758Add
    BLAST

    Proteomic databases

    MaxQBiQ9JHK4.
    PaxDbiQ9JHK4.
    PRIDEiQ9JHK4.

    PTM databases

    PhosphoSiteiQ9JHK4.

    Expressioni

    Gene expression databases

    BgeeiQ9JHK4.
    CleanExiMM_RABGGTA.
    GenevestigatoriQ9JHK4.

    Interactioni

    Subunit structurei

    Heterotrimer composed of RABGGTA, RABGGTB and CHM; within this trimer, RABGGTA and RABGGTB form the catalytic component B, while CHM (component A) mediates peptide substrate binding. The Rab GGTase dimer (RGGT) interacts with CHM (component A) prior to Rab protein binding; the association is stabilized by geranylgeranyl pyrophosphate (GGpp). The CHM:RGGT:Rab complex is destabilized by GGpp By similarity.By similarity

    Protein-protein interaction databases

    BioGridi207827. 1 interaction.
    IntActiQ9JHK4. 1 interaction.
    STRINGi10090.ENSMUSP00000061498.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9JHK4.
    SMRiQ9JHK4. Positions 2-567.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9JHK4.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati44 – 7835PFTA 1Add
    BLAST
    Repeati88 – 12235PFTA 2Add
    BLAST
    Repeati124 – 15835PFTA 3Add
    BLAST
    Repeati159 – 19335PFTA 4Add
    BLAST
    Repeati207 – 24135PFTA 5Add
    BLAST
    Repeati363 – 39735PFTA 6Add
    BLAST
    Repeati442 – 46322LRR 1Add
    BLAST
    Repeati464 – 48623LRR 2Add
    BLAST
    Repeati487 – 50822LRR 3Add
    BLAST
    Repeati509 – 53022LRR 4Add
    BLAST
    Repeati534 – 55522LRR 5Add
    BLAST

    Sequence similaritiesi

    Contains 5 LRR (leucine-rich) repeats.Curated
    Contains 6 PFTA repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Leucine-rich repeat, Repeat

    Phylogenomic databases

    eggNOGiCOG5536.
    GeneTreeiENSGT00550000075121.
    HOGENOMiHOG000007845.
    HOVERGENiHBG002171.
    InParanoidiQ9JHK4.
    KOiK14050.
    OMAiWNCRREV.
    OrthoDBiEOG7PP56C.
    PhylomeDBiQ9JHK4.
    TreeFamiTF315057.

    Family and domain databases

    Gene3Di2.60.40.1130. 1 hit.
    InterProiIPR001611. Leu-rich_rpt.
    IPR002088. Prenyl_trans_a.
    IPR009087. RabGGT_asu_insert-domain.
    [Graphical view]
    PfamiPF00560. LRR_1. 1 hit.
    PF01239. PPTA. 5 hits.
    PF07711. RabGGT_insert. 1 hit.
    [Graphical view]
    ProDomiPD331837. RabGG_trans_A. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF49594. SSF49594. 1 hit.
    PROSITEiPS51147. PFTA. 6 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9JHK4-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MHGRLKVKTS EEQAEAKRLE REQKLKLYQS ATQAVFQKRE AGELDESVLE    50
    LTSQILGANP DFATLWNCRR EVLQQLETQK SPEELAALVK AELGFLESCL 100
    RVNPKSYGTW HHRCWLLSRL PEPNWARELE LCARFLEADE RNFHCWDYRR 150
    FVAAQAAVAP AEELAFTDSL ITRNFSNYSS WHYRSCLLPQ LHPQPDSGPQ 200
    GRLPENVLLR ELELVQNAFF TDPNDQSAWF YHRWLLGRAE PHDVLCCLHV 250
    SREEACLSVC FSRPLIVGSK MGTLLLTVDE APLSVEWRTP DGRNRPSHVW 300
    LCDLPAASLN DHLPQHTFRV IWTGSDTQKE CVLLKGHQEC WCRDSATDEQ 350
    LFRCELSVEK STVLQSELES CKELQELEPE NKWCLLTIIL LMRALDPLLY 400
    EKETLEYFST LKAVDPMRAA YLDDLRSKFL VENSVLKMEY ADVRVLHLAH 450
    KDLTVLCHLE QLLLVTHLDL SHNRLRALPP ALAALRCLEV LQASDNVLEN 500
    LDGVANLPRL RELLLCNNRL QQSAALQTLA SCPRLVFLNL QGNSLCQEEG 550
    IRERLAEMLP SVSSILT 567
    Length:567
    Mass (Da):64,989
    Last modified:October 1, 2000 - v1
    Checksum:i387DA2DAC12C4C0D
    GO
    Isoform 2 (identifier: Q9JHK4-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         337-339: HQE → DAV
         340-567: Missing.

    Show »
    Length:339
    Mass (Da):38,943
    Checksum:iB68BCAADA15ED4D2
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei337 – 3393HQE → DAV in isoform 2. 1 PublicationVSP_009113
    Alternative sequencei340 – 567228Missing in isoform 2. 1 PublicationVSP_009114Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF127654 Genomic DNA. Translation: AAF65918.1.
    AF127655 Genomic DNA. Translation: AAF65919.1.
    AF127656 mRNA. Translation: AAF65920.1.
    AF127658 mRNA. Translation: AAF65921.1.
    AF127659 mRNA. Translation: AAF65922.1.
    AF127660 mRNA. Translation: AAF65923.1.
    AF127662 mRNA. Translation: AAF65924.1.
    AK002625 mRNA. Translation: BAB22240.1.
    CCDSiCCDS36933.1. [Q9JHK4-1]
    RefSeqiNP_062392.1. NM_019519.2. [Q9JHK4-1]
    XP_006519357.1. XM_006519294.1. [Q9JHK4-1]
    XP_006519358.1. XM_006519295.1. [Q9JHK4-1]
    UniGeneiMm.87216.

    Genome annotation databases

    EnsembliENSMUST00000062861; ENSMUSP00000061498; ENSMUSG00000040472. [Q9JHK4-1]
    ENSMUST00000163889; ENSMUSP00000128668; ENSMUSG00000040472. [Q9JHK4-1]
    ENSMUST00000169237; ENSMUSP00000133032; ENSMUSG00000040472. [Q9JHK4-1]
    GeneIDi56187.
    KEGGimmu:56187.
    UCSCiuc007uai.1. mouse. [Q9JHK4-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF127654 Genomic DNA. Translation: AAF65918.1 .
    AF127655 Genomic DNA. Translation: AAF65919.1 .
    AF127656 mRNA. Translation: AAF65920.1 .
    AF127658 mRNA. Translation: AAF65921.1 .
    AF127659 mRNA. Translation: AAF65922.1 .
    AF127660 mRNA. Translation: AAF65923.1 .
    AF127662 mRNA. Translation: AAF65924.1 .
    AK002625 mRNA. Translation: BAB22240.1 .
    CCDSi CCDS36933.1. [Q9JHK4-1 ]
    RefSeqi NP_062392.1. NM_019519.2. [Q9JHK4-1 ]
    XP_006519357.1. XM_006519294.1. [Q9JHK4-1 ]
    XP_006519358.1. XM_006519295.1. [Q9JHK4-1 ]
    UniGenei Mm.87216.

    3D structure databases

    ProteinModelPortali Q9JHK4.
    SMRi Q9JHK4. Positions 2-567.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 207827. 1 interaction.
    IntActi Q9JHK4. 1 interaction.
    STRINGi 10090.ENSMUSP00000061498.

    PTM databases

    PhosphoSitei Q9JHK4.

    Proteomic databases

    MaxQBi Q9JHK4.
    PaxDbi Q9JHK4.
    PRIDEi Q9JHK4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000062861 ; ENSMUSP00000061498 ; ENSMUSG00000040472 . [Q9JHK4-1 ]
    ENSMUST00000163889 ; ENSMUSP00000128668 ; ENSMUSG00000040472 . [Q9JHK4-1 ]
    ENSMUST00000169237 ; ENSMUSP00000133032 ; ENSMUSG00000040472 . [Q9JHK4-1 ]
    GeneIDi 56187.
    KEGGi mmu:56187.
    UCSCi uc007uai.1. mouse. [Q9JHK4-1 ]

    Organism-specific databases

    CTDi 5875.
    MGIi MGI:1860443. Rabggta.

    Phylogenomic databases

    eggNOGi COG5536.
    GeneTreei ENSGT00550000075121.
    HOGENOMi HOG000007845.
    HOVERGENi HBG002171.
    InParanoidi Q9JHK4.
    KOi K14050.
    OMAi WNCRREV.
    OrthoDBi EOG7PP56C.
    PhylomeDBi Q9JHK4.
    TreeFami TF315057.

    Miscellaneous databases

    EvolutionaryTracei Q9JHK4.
    NextBioi 311974.
    PROi Q9JHK4.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9JHK4.
    CleanExi MM_RABGGTA.
    Genevestigatori Q9JHK4.

    Family and domain databases

    Gene3Di 2.60.40.1130. 1 hit.
    InterProi IPR001611. Leu-rich_rpt.
    IPR002088. Prenyl_trans_a.
    IPR009087. RabGGT_asu_insert-domain.
    [Graphical view ]
    Pfami PF00560. LRR_1. 1 hit.
    PF01239. PPTA. 5 hits.
    PF07711. RabGGT_insert. 1 hit.
    [Graphical view ]
    ProDomi PD331837. RabGG_trans_A. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF49594. SSF49594. 1 hit.
    PROSITEi PS51147. PFTA. 6 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Rab geranylgeranyl transferase alpha mutation in the gunmetal mouse reduces Rab prenylation and platelet synthesis."
      Detter J.C., Zhang Q., Mules E.H., Novack E.K., Mishra V.S., Li W., McMurtrie E.B., Tchernev V.T., Wallace M.R., Seabra M.C., Swank R.T., Kingsmore S.K.
      Proc. Natl. Acad. Sci. U.S.A. 97:4144-4149(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2), DISEASE, FUNCTION.
      Strain: C57BL/6J and C57BL/6J-GM/GM.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Kidney.

    Entry informationi

    Entry nameiPGTA_MOUSE
    AccessioniPrimary (citable) accession number: Q9JHK4
    Secondary accession number(s): Q9JLX2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 2003
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3