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Protein

ICOS ligand

Gene

Icoslg

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function. During pregnancy, may function to skew the cytokine of maternal T-cells toward immunoprotective Th2 phenotype.

GO - Molecular functioni

  • receptor binding Source: UniProtKB

GO - Biological processi

  • isotype switching Source: UniProtKB
  • positive regulation of activated T cell proliferation Source: UniProtKB
  • positive regulation of interleukin-4 biosynthetic process Source: UniProtKB
  • signal transduction Source: UniProtKB
  • T cell activation Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Adaptive immunity, B-cell activation, Immunity

Enzyme and pathway databases

ReactomeiR-MMU-388841. Costimulation by the CD28 family.

Names & Taxonomyi

Protein namesi
Recommended name:
ICOS ligand
Alternative name(s):
B7 homolog 2
Short name:
B7-H2
B7-like protein Gl50
B7-related protein 1
Short name:
B7RP-1
LICOS
CD_antigen: CD275
Gene namesi
Name:Icoslg
Synonyms:B7h2, B7rp1, Icosl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1354701. Icosl.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini47 – 277ExtracellularSequence analysisAdd BLAST231
Transmembranei278 – 298HelicalSequence analysisAdd BLAST21
Topological domaini299 – 322CytoplasmicSequence analysisAdd BLAST24

GO - Cellular componenti

  • external side of plasma membrane Source: MGI
  • extracellular exosome Source: MGI
  • integral component of membrane Source: UniProtKB
  • integral component of plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 46By similarityAdd BLAST46
ChainiPRO_000001480447 – 322ICOS ligandAdd BLAST276

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi62 ↔ 138PROSITE-ProRule annotation
Glycosylationi71N-linked (GlcNAc...)Sequence analysis1
Glycosylationi120N-linked (GlcNAc...)Sequence analysis1
Glycosylationi127N-linked (GlcNAc...)Sequence analysis1
Glycosylationi163N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi185 ↔ 243PROSITE-ProRule annotation
Glycosylationi200N-linked (GlcNAc...)Sequence analysis1
Glycosylationi213N-linked (GlcNAc...)Sequence analysis1
Glycosylationi252N-linked (GlcNAc...)Sequence analysis1
Glycosylationi265N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9JHJ8.
PRIDEiQ9JHJ8.

PTM databases

PhosphoSitePlusiQ9JHJ8.

Expressioni

Tissue specificityi

Isoform 1 highest expression in lymphoid tissues, such as spleen (mostly in the marginal zone), lymph nodes (particularly in the cortex and in both primary and secondary follicles), thymus (predominantly in the medulla) and Peyer patches (mostly in the follicles), lower levels in many non-lymphoid tissues, such as brain, heart, kidney, liver, lung, skeletal muscle and testis. Present on freshly isolated splenic B-cells, T-cells, dendritic cells and macrophages. The expression of isoform 2 is restricted to heart, spleen and kidney.

Developmental stagei

Detected early in hemopoiesis: in the yolk sac at 11.5 and 12.5 dpc and, to a lesser extent, in the liver at 14.5 dpc.

Gene expression databases

BgeeiENSMUSG00000000732.
CleanExiMM_ICOSL.
ExpressionAtlasiQ9JHJ8. baseline and differential.
GenevisibleiQ9JHJ8. MM.

Interactioni

GO - Molecular functioni

  • receptor binding Source: UniProtKB

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000101032.

Structurei

3D structure databases

ProteinModelPortaliQ9JHJ8.
SMRiQ9JHJ8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini49 – 149Ig-like V-typeAdd BLAST101
Domaini167 – 263Ig-like C2-typeAdd BLAST97

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi31 – 38Poly-Leu8
Compositional biasi289 – 292Poly-Ala4

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IYI6. Eukaryota.
ENOG410Z67K. LUCA.
GeneTreeiENSGT00830000128435.
HOGENOMiHOG000036913.
HOVERGENiHBG052078.
InParanoidiQ9JHJ8.
KOiK06710.
OMAiLRVYWQI.
OrthoDBiEOG091G0IEZ.
PhylomeDBiQ9JHJ8.
TreeFamiTF331083.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF08205. C2-set_2. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: Q9JHJ8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQLKCPCFVS LGTRQPVWKK LHVSSGFFSG LGLFLLLLSS LCAASAETEV
60 70 80 90 100
GAMVGSNVVL SCIDPHRRHF NLSGLYVYWQ IENPEVSVTY YLPYKSPGIN
110 120 130 140 150
VDSSYKNRGH LSLDSMKQGN FSLYLKNVTP QDTQEFTCRV FMNTATELVK
160 170 180 190 200
ILEEVVRLRV AANFSTPVIS TSDSSNPGQE RTYTCMSKNG YPEPNLYWIN
210 220 230 240 250
TTDNSLIDTA LQNNTVYLNK LGLYDVISTL RLPWTSRGDV LCCVENVALH
260 270 280 290 300
QNITSISQAE SFTGNNTKNP QETHNNELKV LVPVLAVLAA AAFVSFIIYR
310 320
RTRPHRSYTG PKTVQLELTD HA
Length:322
Mass (Da):35,960
Last modified:October 1, 2000 - v1
Checksum:i55CCBA4AD12E47E6
GO
Isoform 2 (identifier: Q9JHJ8-2) [UniParc]FASTAAdd to basket
Also known as: B

The sequence of this isoform differs from the canonical sequence as follows:
     321-322: HA → TWAPVPYQDYLIPRYLMSPCLKTRGLP

Show »
Length:347
Mass (Da):38,914
Checksum:i5AA8163BDCBF45D2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti237R → H in AAK77544 (PubMed:11390480).Curated1
Sequence conflicti237R → H in CAC36464 (Ref. 5) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_002521321 – 322HA → TWAPVPYQDYLIPRYLMSPC LKTRGLP in isoform 2. Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF216747 mRNA. Translation: AAF45149.1.
AF199027 mRNA. Translation: AAF34738.1.
AX100591 Unassigned DNA. Translation: CAC36463.1.
AX100593 Unassigned DNA. Translation: CAC36464.1.
AF394451 mRNA. Translation: AAK77544.1.
BC029227 mRNA. Translation: AAH29227.1.
CCDSiCCDS35956.1. [Q9JHJ8-1]
RefSeqiNP_056605.1. NM_015790.3. [Q9JHJ8-1]
UniGeneiMm.17819.

Genome annotation databases

EnsembliENSMUST00000105393; ENSMUSP00000101032; ENSMUSG00000000732. [Q9JHJ8-1]
GeneIDi50723.
KEGGimmu:50723.
UCSCiuc007fxi.1. mouse. [Q9JHJ8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF216747 mRNA. Translation: AAF45149.1.
AF199027 mRNA. Translation: AAF34738.1.
AX100591 Unassigned DNA. Translation: CAC36463.1.
AX100593 Unassigned DNA. Translation: CAC36464.1.
AF394451 mRNA. Translation: AAK77544.1.
BC029227 mRNA. Translation: AAH29227.1.
CCDSiCCDS35956.1. [Q9JHJ8-1]
RefSeqiNP_056605.1. NM_015790.3. [Q9JHJ8-1]
UniGeneiMm.17819.

3D structure databases

ProteinModelPortaliQ9JHJ8.
SMRiQ9JHJ8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000101032.

PTM databases

PhosphoSitePlusiQ9JHJ8.

Proteomic databases

PaxDbiQ9JHJ8.
PRIDEiQ9JHJ8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000105393; ENSMUSP00000101032; ENSMUSG00000000732. [Q9JHJ8-1]
GeneIDi50723.
KEGGimmu:50723.
UCSCiuc007fxi.1. mouse. [Q9JHJ8-1]

Organism-specific databases

CTDi50723.
MGIiMGI:1354701. Icosl.

Phylogenomic databases

eggNOGiENOG410IYI6. Eukaryota.
ENOG410Z67K. LUCA.
GeneTreeiENSGT00830000128435.
HOGENOMiHOG000036913.
HOVERGENiHBG052078.
InParanoidiQ9JHJ8.
KOiK06710.
OMAiLRVYWQI.
OrthoDBiEOG091G0IEZ.
PhylomeDBiQ9JHJ8.
TreeFamiTF331083.

Enzyme and pathway databases

ReactomeiR-MMU-388841. Costimulation by the CD28 family.

Miscellaneous databases

PROiQ9JHJ8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000000732.
CleanExiMM_ICOSL.
ExpressionAtlasiQ9JHJ8. baseline and differential.
GenevisibleiQ9JHJ8. MM.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF08205. C2-set_2. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiICOSL_MOUSE
AccessioniPrimary (citable) accession number: Q9JHJ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 14, 2001
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.