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Protein

ICOS ligand

Gene

Icoslg

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function. During pregnancy, may function to skew the cytokine of maternal T-cells toward immunoprotective Th2 phenotype.

GO - Molecular functioni

  • receptor binding Source: UniProtKB

GO - Biological processi

  • isotype switching Source: UniProtKB
  • positive regulation of activated T cell proliferation Source: UniProtKB
  • positive regulation of interleukin-4 biosynthetic process Source: UniProtKB
  • signal transduction Source: UniProtKB
  • T cell activation Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Adaptive immunity, B-cell activation, Immunity

Enzyme and pathway databases

ReactomeiR-MMU-388841. Costimulation by the CD28 family.

Names & Taxonomyi

Protein namesi
Recommended name:
ICOS ligand
Alternative name(s):
B7 homolog 2
Short name:
B7-H2
B7-like protein Gl50
B7-related protein 1
Short name:
B7RP-1
LICOS
CD_antigen: CD275
Gene namesi
Name:Icoslg
Synonyms:B7h2, B7rp1, Icosl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1354701. Icosl.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini47 – 277231ExtracellularSequence analysisAdd
BLAST
Transmembranei278 – 29821HelicalSequence analysisAdd
BLAST
Topological domaini299 – 32224CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • external side of plasma membrane Source: MGI
  • extracellular exosome Source: MGI
  • integral component of membrane Source: UniProtKB
  • integral component of plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4646By similarityAdd
BLAST
Chaini47 – 322276ICOS ligandPRO_0000014804Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi62 ↔ 138PROSITE-ProRule annotation
Glycosylationi71 – 711N-linked (GlcNAc...)Sequence analysis
Glycosylationi120 – 1201N-linked (GlcNAc...)Sequence analysis
Glycosylationi127 – 1271N-linked (GlcNAc...)Sequence analysis
Glycosylationi163 – 1631N-linked (GlcNAc...)Sequence analysis
Disulfide bondi185 ↔ 243PROSITE-ProRule annotation
Glycosylationi200 – 2001N-linked (GlcNAc...)Sequence analysis
Glycosylationi213 – 2131N-linked (GlcNAc...)Sequence analysis
Glycosylationi252 – 2521N-linked (GlcNAc...)Sequence analysis
Glycosylationi265 – 2651N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ9JHJ8.
PaxDbiQ9JHJ8.
PRIDEiQ9JHJ8.

Expressioni

Tissue specificityi

Isoform 1 highest expression in lymphoid tissues, such as spleen (mostly in the marginal zone), lymph nodes (particularly in the cortex and in both primary and secondary follicles), thymus (predominantly in the medulla) and Peyer patches (mostly in the follicles), lower levels in many non-lymphoid tissues, such as brain, heart, kidney, liver, lung, skeletal muscle and testis. Present on freshly isolated splenic B-cells, T-cells, dendritic cells and macrophages. The expression of isoform 2 is restricted to heart, spleen and kidney.

Developmental stagei

Detected early in hemopoiesis: in the yolk sac at 11.5 and 12.5 dpc and, to a lesser extent, in the liver at 14.5 dpc.

Gene expression databases

BgeeiQ9JHJ8.
CleanExiMM_ICOSL.
ExpressionAtlasiQ9JHJ8. baseline and differential.
GenevisibleiQ9JHJ8. MM.

Interactioni

GO - Molecular functioni

  • receptor binding Source: UniProtKB

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000101032.

Structurei

3D structure databases

ProteinModelPortaliQ9JHJ8.
SMRiQ9JHJ8. Positions 47-252.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini49 – 149101Ig-like V-typeAdd
BLAST
Domaini167 – 26397Ig-like C2-typeAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi31 – 388Poly-Leu
Compositional biasi289 – 2924Poly-Ala

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IYI6. Eukaryota.
ENOG410Z67K. LUCA.
GeneTreeiENSGT00830000128435.
HOGENOMiHOG000036913.
HOVERGENiHBG052078.
InParanoidiQ9JHJ8.
KOiK06710.
OMAiLRVYWQI.
OrthoDBiEOG79KPFZ.
PhylomeDBiQ9JHJ8.
TreeFamiTF331083.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF08205. C2-set_2. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: Q9JHJ8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQLKCPCFVS LGTRQPVWKK LHVSSGFFSG LGLFLLLLSS LCAASAETEV
60 70 80 90 100
GAMVGSNVVL SCIDPHRRHF NLSGLYVYWQ IENPEVSVTY YLPYKSPGIN
110 120 130 140 150
VDSSYKNRGH LSLDSMKQGN FSLYLKNVTP QDTQEFTCRV FMNTATELVK
160 170 180 190 200
ILEEVVRLRV AANFSTPVIS TSDSSNPGQE RTYTCMSKNG YPEPNLYWIN
210 220 230 240 250
TTDNSLIDTA LQNNTVYLNK LGLYDVISTL RLPWTSRGDV LCCVENVALH
260 270 280 290 300
QNITSISQAE SFTGNNTKNP QETHNNELKV LVPVLAVLAA AAFVSFIIYR
310 320
RTRPHRSYTG PKTVQLELTD HA
Length:322
Mass (Da):35,960
Last modified:October 1, 2000 - v1
Checksum:i55CCBA4AD12E47E6
GO
Isoform 2 (identifier: Q9JHJ8-2) [UniParc]FASTAAdd to basket

Also known as: B

The sequence of this isoform differs from the canonical sequence as follows:
     321-322: HA → TWAPVPYQDYLIPRYLMSPCLKTRGLP

Show »
Length:347
Mass (Da):38,914
Checksum:i5AA8163BDCBF45D2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti237 – 2371R → H in AAK77544 (PubMed:11390480).Curated
Sequence conflicti237 – 2371R → H in CAC36464 (Ref. 5) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei321 – 3222HA → TWAPVPYQDYLIPRYLMSPC LKTRGLP in isoform 2. CuratedVSP_002521

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF216747 mRNA. Translation: AAF45149.1.
AF199027 mRNA. Translation: AAF34738.1.
AX100591 Unassigned DNA. Translation: CAC36463.1.
AX100593 Unassigned DNA. Translation: CAC36464.1.
AF394451 mRNA. Translation: AAK77544.1.
BC029227 mRNA. Translation: AAH29227.1.
CCDSiCCDS35956.1. [Q9JHJ8-1]
RefSeqiNP_056605.1. NM_015790.3. [Q9JHJ8-1]
UniGeneiMm.17819.

Genome annotation databases

EnsembliENSMUST00000105393; ENSMUSP00000101032; ENSMUSG00000000732. [Q9JHJ8-1]
GeneIDi50723.
KEGGimmu:50723.
UCSCiuc007fxi.1. mouse. [Q9JHJ8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF216747 mRNA. Translation: AAF45149.1.
AF199027 mRNA. Translation: AAF34738.1.
AX100591 Unassigned DNA. Translation: CAC36463.1.
AX100593 Unassigned DNA. Translation: CAC36464.1.
AF394451 mRNA. Translation: AAK77544.1.
BC029227 mRNA. Translation: AAH29227.1.
CCDSiCCDS35956.1. [Q9JHJ8-1]
RefSeqiNP_056605.1. NM_015790.3. [Q9JHJ8-1]
UniGeneiMm.17819.

3D structure databases

ProteinModelPortaliQ9JHJ8.
SMRiQ9JHJ8. Positions 47-252.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000101032.

Proteomic databases

MaxQBiQ9JHJ8.
PaxDbiQ9JHJ8.
PRIDEiQ9JHJ8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000105393; ENSMUSP00000101032; ENSMUSG00000000732. [Q9JHJ8-1]
GeneIDi50723.
KEGGimmu:50723.
UCSCiuc007fxi.1. mouse. [Q9JHJ8-1]

Organism-specific databases

CTDi50723.
MGIiMGI:1354701. Icosl.

Phylogenomic databases

eggNOGiENOG410IYI6. Eukaryota.
ENOG410Z67K. LUCA.
GeneTreeiENSGT00830000128435.
HOGENOMiHOG000036913.
HOVERGENiHBG052078.
InParanoidiQ9JHJ8.
KOiK06710.
OMAiLRVYWQI.
OrthoDBiEOG79KPFZ.
PhylomeDBiQ9JHJ8.
TreeFamiTF331083.

Enzyme and pathway databases

ReactomeiR-MMU-388841. Costimulation by the CD28 family.

Miscellaneous databases

NextBioi307581.
PROiQ9JHJ8.
SOURCEiSearch...

Gene expression databases

BgeeiQ9JHJ8.
CleanExiMM_ICOSL.
ExpressionAtlasiQ9JHJ8. baseline and differential.
GenevisibleiQ9JHJ8. MM.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF08205. C2-set_2. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1), CHARACTERIZATION.
    Tissue: Lymphocyte.
  2. "B7h, a novel costimulatory homolog of B7.1 and B7.2, is induced by TNFalpha."
    Swallow M.M., Wallin J.J., Sha W.C.
    Immunity 11:423-432(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1).
    Tissue: Thymus.
  3. Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1).
    Strain: C3H/HeJ.
    Tissue: Fetal thymus.
  4. "Differential expression of inducible costimulator-ligand splice variants: lymphoid regulation of mouse gl50-b and human gl50 molecules."
    Ling V., Wu P.W., Miyashiro J.S., Marusic S., Finnerty H.F., Collins M.
    J. Immunol. 166:7300-7308(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 2).
    Tissue: Peripheral blood lymphocyte.
  5. Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS 1 AND 2).
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Colon.

Entry informationi

Entry nameiICOSL_MOUSE
AccessioniPrimary (citable) accession number: Q9JHJ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 14, 2001
Last sequence update: October 1, 2000
Last modified: February 17, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.