Q9JHJ5 (5HT3B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-hydroxytryptamine receptor 3B
Short name=5-HT3-B
Short name=5-HT3B

Alternative name(s):
Serotonin receptor 3B

Gene names

Name:

Htr3b

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	437 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses. It is a cation-specific, but otherwise relatively nonselective, ion channel. Ref.4
Subunit structure	Forms a pentaheteromeric complex with HTR3A. Not functional as a homomeric complex By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein. Note: Presumably retained within the endoplasmic reticulum unless complexed with HTR3A By similarity.
Post-translational modification	N-glycosylation is required for membrane localization By similarity.
Sequence similarities	Belongs to the ligand-gated ion channel (TC 1.A.9) family. 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3B sub-subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell membrane Membrane
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Ionic channel Ligand-gated ion channel Receptor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to plasma membrane Inferred from direct assay Ref.1. Source: MGI postsynaptic membrane Inferred from electronic annotation. Source: InterPro
Molecular function	serotonin receptor activity Inferred from direct assay Ref.1. Source: MGI serotonin-activated cation-selective channel activity Inferred from direct assay Ref.1. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 437	416	5-hydroxytryptamine receptor 3B		PRO_0000312290
Regions
	Topological domain	22 – 238	217	Extracellular Potential
	Transmembrane	239 – 259	21	Helical; Name=1; Potential
	Topological domain	260 – 264	5	Cytoplasmic Potential
	Transmembrane	265 – 282	18	Helical; Name=2; Potential
	Topological domain	283 – 292	10	Extracellular Potential
	Transmembrane	293 – 313	21	Helical; Name=3; Potential
	Topological domain	314 – 410	97	Cytoplasmic Potential
	Transmembrane	411 – 431	21	Helical; Name=4; Potential
	Topological domain	432 – 437	6	Extracellular Potential
	Region	377 – 409	33	HA-stretch
Amino acid modifications
	Glycosylation	25	1	N-linked (GlcNAc...) Potential
	Glycosylation	92	1	N-linked (GlcNAc...) Potential
	Glycosylation	134	1	N-linked (GlcNAc...) Potential
	Glycosylation	283	1	N-linked (GlcNAc...) Potential
	Disulfide bond	151 ↔ 165		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9JHJ5 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 60019008AF23F0FC	FASTA	437	50,321
	10 20 30 40 50 60 MILLWSCLLV AVVGILGTAT PQPGNSSLHR LTRQLLQQYH KEVRPVYNWA EATTVYLDLC 70 80 90 100 110 120 VHAVLDVDVQ NQKLKTSVWY REVWNDEFLS WNSSLFDEIQ EISLPLSALW APDIIINEFV 130 140 150 160 170 180 DVERSPDLPY VYVNSSGTIR NHKPIQVVSA CSLQTYAFPF DIQNCSLTFN SILHTVEDID 190 200 210 220 230 240 LGFLRNREDI ENDKRAFMND SEWQLLSVSS TYHIRQSSAG DFAQIRFNVV IRRCPLAYVV 250 260 270 280 290 300 SLLIPSIFLM LVDLGSFYLP PNCRARIVFK TNVLVGYTVF RVNMSDEVPR SAGCTPLIGV 310 320 330 340 350 360 FFTVCMALLV LSLSKSILLI KFLYEERHSG QERPLMCLQG DSDAEESRLY LGAPRADVTE 370 380 390 400 410 420 SPVHQEHRVP SDTLKDFWFQ FRSINNSLRT RDQIHQKEVE WLAILYRFDQ LLFRIYLAVL 430 GLYTVTLCSL WALWSRM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Evidence for expression of heteromeric serotonin 5-HT(3) receptors in rodents." Hanna M.C., Davies P.A., Hales T.G., Kirkness E.F. J. Neurochem. 75:240-247(2000) [PubMed: 10854267] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: 129/SvJ and BALB/c.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Stomach.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Olfactory epithelium.
[4]	"A cytoplasmic region determines single-channel conductance in 5-HT3 receptors." Kelley S.P., Dunlop J.I., Kirkness E.F., Lambert J.J., Peters J.A. Nature 424:321-324(2003) [PubMed: 12867984] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF155045 mRNA. Translation: AAF73284.1. AF155052 , AF155046, AF155047, AF155048, AF155049, AF155050, AF155051 Genomic DNA. Translation: AAF73285.1. AK085213 mRNA. Translation: BAC39391.1. BC046990 mRNA. Translation: AAH46990.1.
IPI	IPI00119487.
RefSeq	NP_064670.1. NM_020274.4.
UniGene	Mm.117131.
3D structure databases
HSSP	HSSP built from PDB template 2BG9 based on UniProtKB P02711.
ProteinModelPortal	Q9JHJ5.
SMR	Q9JHJ5. Positions 26-430.
ModBase	Search...
Proteomic databases
PRIDE	Q9JHJ5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000008734; ENSMUSP00000008734; ENSMUSG00000008590.
GeneID	57014.
KEGG	mmu:57014.
UCSC	uc012gsr.1. mouse.
Organism-specific databases
CTD	9177.
MGI	MGI:1861899. Htr3b.
Phylogenomic databases
eggNOG	roNOG05248.
GeneTree	ENSGT00570000078866.
HOGENOM	HBG714660.
HOVERGEN	HBG106638.
InParanoid	Q9JHJ5.
OMA	RSREDIK.
OrthoDB	EOG4N8R4Z.
PhylomeDB	Q9JHJ5.
Gene expression databases
ArrayExpress	Q9JHJ5.
Bgee	Q9JHJ5.
CleanEx	MM_HTR3B.
Genevestigator	Q9JHJ5.
Family and domain databases
InterPro	IPR008132. 5HT3_rcpt. IPR008134. 5HT3_rcpt_B. IPR006202. Neur_chan_lig-bd. IPR006201. Neur_channel. IPR006029. Neurotrans-gated_channel_TM. [Graphical view]
Gene3D	G3DSA:2.70.170.10. Neur_chan_lig_bd. 1 hit.
KO	K04819.
PANTHER	PTHR18945. Neur_channel. 1 hit.
Pfam	PF02931. Neur_chan_LBD. 1 hit. PF02932. Neur_chan_memb. 1 hit. [Graphical view]
PRINTS	PR01710. 5HT3BRECEPTR. PR01708. 5HT3RECEPTOR. PR00252. NRIONCHANNEL.
SUPFAM	SSF90112. Neu_channel_TM. 1 hit. SSF63712. Neur_chan_LBD. 1 hit.
TIGRFAMs	TIGR00860. LIC. 1 hit.
PROSITE	PS00236. NEUROTR_ION_CHANNEL. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	313469.
SOURCE	Search...

Entry information

Entry name

5HT3B_MOUSE

Accession

Primary (citable) accession number: Q9JHJ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 4, 2007
Last sequence update:	October 1, 2000
Last modified:	November 16, 2011
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program