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Protein

5-hydroxytryptamine receptor 3B

Gene

Htr3b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses. It is a cation-specific, but otherwise relatively nonselective, ion channel.1 Publication

GO - Molecular functioni

  • G-protein coupled serotonin receptor activity Source: MGI
  • serotonin-activated cation-selective channel activity Source: MGI

GO - Biological processi

  • cation transmembrane transport Source: GOC
  • serotonin receptor signaling pathway Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-975298. Ligand-gated ion channel transport.

Names & Taxonomyi

Protein namesi
Recommended name:
5-hydroxytryptamine receptor 3B
Short name:
5-HT3-B
Short name:
5-HT3B
Alternative name(s):
Serotonin receptor 3B
Gene namesi
Name:Htr3b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1861899. Htr3b.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini22 – 238217ExtracellularSequence analysisAdd
BLAST
Transmembranei239 – 25921Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini260 – 2645CytoplasmicSequence analysis
Transmembranei265 – 28218Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini283 – 29210ExtracellularSequence analysis
Transmembranei293 – 31321Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini314 – 41097CytoplasmicSequence analysisAdd
BLAST
Transmembranei411 – 43121Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini432 – 4376ExtracellularSequence analysis

GO - Cellular componenti

  • cell surface Source: MGI
  • integral component of plasma membrane Source: MGI
  • postsynaptic membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2111333.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence analysisAdd
BLAST
Chaini22 – 4374165-hydroxytryptamine receptor 3BPRO_0000312290Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi25 – 251N-linked (GlcNAc...)Sequence analysis
Glycosylationi92 – 921N-linked (GlcNAc...)Sequence analysis
Glycosylationi134 – 1341N-linked (GlcNAc...)Sequence analysis
Disulfide bondi151 ↔ 165By similarity
Glycosylationi283 – 2831N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

N-glycosylation is required for membrane localization.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9JHJ5.
PRIDEiQ9JHJ5.

PTM databases

PhosphoSiteiQ9JHJ5.

Expressioni

Gene expression databases

BgeeiQ9JHJ5.
CleanExiMM_HTR3B.
GenevisibleiQ9JHJ5. MM.

Interactioni

Subunit structurei

Forms a pentaheteromeric complex with HTR3A. Not functional as a homomeric complex (By similarity).By similarity

Protein-protein interaction databases

IntActiQ9JHJ5. 1 interaction.
STRINGi10090.ENSMUSP00000008734.

Chemistry

BindingDBiQ9JHJ5.

Structurei

3D structure databases

ProteinModelPortaliQ9JHJ5.
SMRiQ9JHJ5. Positions 27-434.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni377 – 40933HA-stretchAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3645. Eukaryota.
ENOG410XQGR. LUCA.
GeneTreeiENSGT00790000122957.
HOGENOMiHOG000241519.
HOVERGENiHBG106638.
InParanoidiQ9JHJ5.
KOiK04819.
OMAiFDRLLFQ.
OrthoDBiEOG7FXZZK.
PhylomeDBiQ9JHJ5.
TreeFamiTF315605.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR008132. 5HT3_rcpt.
IPR008134. 5HT3_rcpt_B.
IPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR01710. 5HT3BRECEPTR.
PR01708. 5HT3RECEPTOR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9JHJ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILLWSCLLV AVVGILGTAT PQPGNSSLHR LTRQLLQQYH KEVRPVYNWA
60 70 80 90 100
EATTVYLDLC VHAVLDVDVQ NQKLKTSVWY REVWNDEFLS WNSSLFDEIQ
110 120 130 140 150
EISLPLSALW APDIIINEFV DVERSPDLPY VYVNSSGTIR NHKPIQVVSA
160 170 180 190 200
CSLQTYAFPF DIQNCSLTFN SILHTVEDID LGFLRNREDI ENDKRAFMND
210 220 230 240 250
SEWQLLSVSS TYHIRQSSAG DFAQIRFNVV IRRCPLAYVV SLLIPSIFLM
260 270 280 290 300
LVDLGSFYLP PNCRARIVFK TNVLVGYTVF RVNMSDEVPR SAGCTPLIGV
310 320 330 340 350
FFTVCMALLV LSLSKSILLI KFLYEERHSG QERPLMCLQG DSDAEESRLY
360 370 380 390 400
LGAPRADVTE SPVHQEHRVP SDTLKDFWFQ FRSINNSLRT RDQIHQKEVE
410 420 430
WLAILYRFDQ LLFRIYLAVL GLYTVTLCSL WALWSRM
Length:437
Mass (Da):50,321
Last modified:October 1, 2000 - v1
Checksum:i60019008AF23F0FC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF155045 mRNA. Translation: AAF73284.1.
AF155052
, AF155046, AF155047, AF155048, AF155049, AF155050, AF155051 Genomic DNA. Translation: AAF73285.1.
AK085213 mRNA. Translation: BAC39391.1.
BC046990 mRNA. Translation: AAH46990.1.
CCDSiCCDS23160.1.
RefSeqiNP_064670.1. NM_020274.4.
UniGeneiMm.117131.

Genome annotation databases

EnsembliENSMUST00000008734; ENSMUSP00000008734; ENSMUSG00000008590.
GeneIDi57014.
KEGGimmu:57014.
UCSCiuc012gsr.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF155045 mRNA. Translation: AAF73284.1.
AF155052
, AF155046, AF155047, AF155048, AF155049, AF155050, AF155051 Genomic DNA. Translation: AAF73285.1.
AK085213 mRNA. Translation: BAC39391.1.
BC046990 mRNA. Translation: AAH46990.1.
CCDSiCCDS23160.1.
RefSeqiNP_064670.1. NM_020274.4.
UniGeneiMm.117131.

3D structure databases

ProteinModelPortaliQ9JHJ5.
SMRiQ9JHJ5. Positions 27-434.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9JHJ5. 1 interaction.
STRINGi10090.ENSMUSP00000008734.

Chemistry

BindingDBiQ9JHJ5.
ChEMBLiCHEMBL2111333.

PTM databases

PhosphoSiteiQ9JHJ5.

Proteomic databases

PaxDbiQ9JHJ5.
PRIDEiQ9JHJ5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000008734; ENSMUSP00000008734; ENSMUSG00000008590.
GeneIDi57014.
KEGGimmu:57014.
UCSCiuc012gsr.1. mouse.

Organism-specific databases

CTDi9177.
MGIiMGI:1861899. Htr3b.

Phylogenomic databases

eggNOGiKOG3645. Eukaryota.
ENOG410XQGR. LUCA.
GeneTreeiENSGT00790000122957.
HOGENOMiHOG000241519.
HOVERGENiHBG106638.
InParanoidiQ9JHJ5.
KOiK04819.
OMAiFDRLLFQ.
OrthoDBiEOG7FXZZK.
PhylomeDBiQ9JHJ5.
TreeFamiTF315605.

Enzyme and pathway databases

ReactomeiR-MMU-975298. Ligand-gated ion channel transport.

Miscellaneous databases

NextBioi313469.
PROiQ9JHJ5.
SOURCEiSearch...

Gene expression databases

BgeeiQ9JHJ5.
CleanExiMM_HTR3B.
GenevisibleiQ9JHJ5. MM.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR008132. 5HT3_rcpt.
IPR008134. 5HT3_rcpt_B.
IPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR01710. 5HT3BRECEPTR.
PR01708. 5HT3RECEPTOR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Evidence for expression of heteromeric serotonin 5-HT(3) receptors in rodents."
    Hanna M.C., Davies P.A., Hales T.G., Kirkness E.F.
    J. Neurochem. 75:240-247(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: 129/SvJ and BALB/cJ.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Stomach.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Olfactory epithelium.
  4. "A cytoplasmic region determines single-channel conductance in 5-HT3 receptors."
    Kelley S.P., Dunlop J.I., Kirkness E.F., Lambert J.J., Peters J.A.
    Nature 424:321-324(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry namei5HT3B_MOUSE
AccessioniPrimary (citable) accession number: Q9JHJ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: October 1, 2000
Last modified: April 13, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.