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Protein

Carboxypeptidase B2

Gene

Cpb2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. Down-regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin.

Catalytic activityi

Release of C-terminal Arg and Lys from a polypeptide.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Enzyme regulationi

TAFI/CPB2 is unique among carboxypeptidases in that it spontaneously inactivates with a short half-life, a property that is crucial for its role in controlling blood clot lysis. The zymogen is stabilized by interactions with the activation peptide. Release of the activation peptide increases a dynamic flap mobility and in time this leads to conformational changes that disrupt the catalytic site and expose a cryptic thrombin-cleavage site present at Arg-323 (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi180Zinc; catalyticBy similarity1
Metal bindingi183Zinc; catalyticBy similarity1
Binding sitei238SubstrateBy similarity1
Metal bindingi309Zinc; catalyticBy similarity1
Binding sitei362SubstrateBy similarity1
Active sitei384Proton donor/acceptorBy similarity1

GO - Molecular functioni

  • carboxypeptidase activity Source: MGI
  • metallocarboxypeptidase activity Source: GO_Central
  • zinc ion binding Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Biological processi

Blood coagulation, Fibrinolysis, Hemostasis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.17.20. 3474.

Protein family/group databases

MEROPSiM14.009.

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxypeptidase B2 (EC:3.4.17.20)
Alternative name(s):
Carboxypeptidase R
Short name:
CPR
Carboxypeptidase U
Short name:
CPU
Thrombin-activable fibrinolysis inhibitor
Short name:
TAFI
Gene namesi
Name:Cpb2
Synonyms:Tafi
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1891837. Cpb2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
PropeptideiPRO_000000437922 – 113Activation peptideBy similarityAdd BLAST92
ChainiPRO_0000004380114 – 422Carboxypeptidase B2Add BLAST309

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi43N-linked (GlcNAc...)Sequence analysis1
Glycosylationi72N-linked (GlcNAc...)1 Publication1
Glycosylationi84N-linked (GlcNAc...)Sequence analysis1
Glycosylationi107N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi177 ↔ 190By similarity
Glycosylationi240N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi249 ↔ 273By similarity
Disulfide bondi264 ↔ 278By similarity
Glycosylationi322N-linked (GlcNAc...)Sequence analysis1

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei323 – 324Cleavage; by thrombinBy similarity2

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiQ9JHH6.
PaxDbiQ9JHH6.
PeptideAtlasiQ9JHH6.
PRIDEiQ9JHH6.

PTM databases

iPTMnetiQ9JHH6.
PhosphoSitePlusiQ9JHH6.

Expressioni

Tissue specificityi

Plasma; synthesized in the liver.1 Publication

Gene expression databases

BgeeiENSMUSG00000021999.
CleanExiMM_CPB2.
GenevisibleiQ9JHH6. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000022576.

Structurei

3D structure databases

ProteinModelPortaliQ9JHH6.
SMRiQ9JHH6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni180 – 183Substrate bindingBy similarity4
Regioni255 – 256Substrate bindingBy similarity2
Regioni310 – 311Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the peptidase M14 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2650. Eukaryota.
COG2866. LUCA.
GeneTreeiENSGT00760000119103.
HOGENOMiHOG000252968.
HOVERGENiHBG050815.
InParanoidiQ9JHH6.
KOiK01300.
OMAiLRRNINH.
OrthoDBiEOG091G0HUI.
PhylomeDBiQ9JHH6.
TreeFamiTF317197.

Family and domain databases

CDDicd06246. M14_CPB2. 1 hit.
Gene3Di3.30.70.340. 1 hit.
InterProiIPR033849. CPB2.
IPR003146. M14A_act_pep.
IPR000834. Peptidase_M14.
IPR009020. Propept_inh.
[Graphical view]
PfamiPF00246. Peptidase_M14. 1 hit.
PF02244. Propep_M14. 1 hit.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00631. Zn_pept. 1 hit.
[Graphical view]
SUPFAMiSSF54897. SSF54897. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9JHH6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLHGLGILV AIILYEQHGF AFQSGQVLSA LPRTSRQVQL LQNLTTTYEV
60 70 80 90 100
VLWQPVTAEF IEKKKEVHFF VNASDVDSVK AHLNVSRIPF NVLMNNVEDL
110 120 130 140 150
IEQQTFNDTV SPRASASYYE QYHSLNEIYS WIEVITEQHP DMLQKIYIGS
160 170 180 190 200
SFEKYPLYVL KVSGKEQRIK NAIWIDCGIH AREWISPAFC LWFIGYVTQF
210 220 230 240 250
HGKENLYTRL LRHVDFYIMP VMNVDGYDYT WKKNRMWRKN RSAHKNNRCV
260 270 280 290 300
GTDLNRNFAS KHWCEKGASS SSCSETYCGL YPESEPEVKA VADFLRRNID
310 320 330 340 350
HIKAYISMHS YSQQILFPYS YNRSKSKDHE ELSLVASEAV RAIESINKNT
360 370 380 390 400
RYTHGSGSES LYLAPGGSDD WIYDLGIKYS FTIELRDTGR YGFLLPERYI
410 420
KPTCAEALAA ISKIVWHVIR NT
Length:422
Mass (Da):48,871
Last modified:October 1, 2000 - v1
Checksum:i99113755669D55CB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti135I → L in AAF00528 (Ref. 3) Curated1
Sequence conflicti277Y → C in AAH89577 (PubMed:15489334).Curated1
Sequence conflicti344 – 346ESI → GSF in AAF00528 (Ref. 3) Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB021968 mRNA. Translation: BAB03402.1.
AF164524 mRNA. Translation: AAF62385.1.
AF186188 mRNA. Translation: AAF00528.1.
AK004045 mRNA. Translation: BAB23141.1.
BC089577 mRNA. Translation: AAH89577.1.
CCDSiCCDS27277.1.
RefSeqiNP_062749.2. NM_019775.3.
UniGeneiMm.24242.

Genome annotation databases

EnsembliENSMUST00000022576; ENSMUSP00000022576; ENSMUSG00000021999.
GeneIDi56373.
KEGGimmu:56373.
UCSCiuc007uqq.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB021968 mRNA. Translation: BAB03402.1.
AF164524 mRNA. Translation: AAF62385.1.
AF186188 mRNA. Translation: AAF00528.1.
AK004045 mRNA. Translation: BAB23141.1.
BC089577 mRNA. Translation: AAH89577.1.
CCDSiCCDS27277.1.
RefSeqiNP_062749.2. NM_019775.3.
UniGeneiMm.24242.

3D structure databases

ProteinModelPortaliQ9JHH6.
SMRiQ9JHH6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000022576.

Protein family/group databases

MEROPSiM14.009.

PTM databases

iPTMnetiQ9JHH6.
PhosphoSitePlusiQ9JHH6.

Proteomic databases

MaxQBiQ9JHH6.
PaxDbiQ9JHH6.
PeptideAtlasiQ9JHH6.
PRIDEiQ9JHH6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022576; ENSMUSP00000022576; ENSMUSG00000021999.
GeneIDi56373.
KEGGimmu:56373.
UCSCiuc007uqq.2. mouse.

Organism-specific databases

CTDi1361.
MGIiMGI:1891837. Cpb2.

Phylogenomic databases

eggNOGiKOG2650. Eukaryota.
COG2866. LUCA.
GeneTreeiENSGT00760000119103.
HOGENOMiHOG000252968.
HOVERGENiHBG050815.
InParanoidiQ9JHH6.
KOiK01300.
OMAiLRRNINH.
OrthoDBiEOG091G0HUI.
PhylomeDBiQ9JHH6.
TreeFamiTF317197.

Enzyme and pathway databases

BRENDAi3.4.17.20. 3474.

Miscellaneous databases

PROiQ9JHH6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021999.
CleanExiMM_CPB2.
GenevisibleiQ9JHH6. MM.

Family and domain databases

CDDicd06246. M14_CPB2. 1 hit.
Gene3Di3.30.70.340. 1 hit.
InterProiIPR033849. CPB2.
IPR003146. M14A_act_pep.
IPR000834. Peptidase_M14.
IPR009020. Propept_inh.
[Graphical view]
PfamiPF00246. Peptidase_M14. 1 hit.
PF02244. Propep_M14. 1 hit.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00631. Zn_pept. 1 hit.
[Graphical view]
SUPFAMiSSF54897. SSF54897. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCBPB2_MOUSE
AccessioniPrimary (citable) accession number: Q9JHH6
Secondary accession number(s): Q5EBI3, Q9QZF0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.