ID   TSN32_MOUSE             Reviewed;         256 AA.
AC   Q9JHH2; Q923G9; Q9ESH0; Q9JHQ9; Q9JHR0; Q9JHR1; Q9JHR2; Q9JHS8;
DT   27-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT   27-MAR-2002, sequence version 2.
DT   24-JAN-2024, entry version 147.
DE   RecName: Full=Tetraspanin-32;
DE            Short=Tspan-32;
DE   AltName: Full=AML1-regulated transmembrane protein 1;
DE   AltName: Full=Protein Phemx;
GN   Name=Tspan32; Synonyms=Art1, Phemx, Tssc6;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, AND
RP   DEVELOPMENTAL STAGE.
RC   STRAIN=C57BL/6J;
RX   PubMed=10950922; DOI=10.1006/geno.2000.6277;
RA   Nicholson R.H., Pantano S., Eliason J.F., Galy A., Weiler S., Kaplan J.,
RA   Hughes M.R., Ko M.S.H.;
RT   "Phemx, a novel mouse gene expressed in hematopoietic cells maps to the
RT   imprinted cluster on distal chromosome 7.";
RL   Genomics 68:13-21(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1; 2; 3; 4 AND 5).
RC   STRAIN=129/Sv, and C57BL/6J;
RX   PubMed=10915772; DOI=10.1093/hmg/9.12.1829;
RA   Paulsen M., El-Maarri O., Engemann S., Stroedicke M., Franck O., Davies K.,
RA   Reinhardt R., Reik W., Walter J.;
RT   "Sequence conservation and variability of imprinting in the Beckwith-
RT   Wiedemann syndrome gene cluster in human and mouse.";
RL   Hum. Mol. Genet. 9:1829-1841(2000).
RN   [3]
RP   NUCLEOTIDE SEQUENCE (ISOFORM 6).
RX   PubMed=11718897; DOI=10.1016/s0167-4781(01)00306-2;
RA   Robb L., Tarrant J., Groom J., Ibrahim M., Li R., Borobakas B.,
RA   Wright M.D.;
RT   "Molecular characterisation of mouse and human TSSC6: evidence that TSSC6
RT   is a genuine member of the tetraspanin superfamily and is expressed
RT   specifically in haematopoietic organs.";
RL   Biochim. Biophys. Acta 1522:31-41(2001).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC   STRAIN=C57BL/6J; TISSUE=Spleen;
RX   PubMed=11396961; DOI=10.1006/bbrc.2001.5005;
RA   Harada Y., Harada H., Downing J.R., Kimura A.;
RT   "A hematopoietic-specific transmembrane protein, Art-1, is possibly
RT   regulated by aml1.";
RL   Biochem. Biophys. Res. Commun. 284:714-722(2001).
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=6;
CC         Comment=Additional isoforms seem to exist.;
CC       Name=6;
CC         IsoId=Q9JHH2-1; Sequence=Displayed;
CC       Name=1;
CC         IsoId=Q9JHH2-2; Sequence=VSP_003939;
CC       Name=2;
CC         IsoId=Q9JHH2-3; Sequence=VSP_003939, VSP_003941;
CC       Name=3;
CC         IsoId=Q9JHH2-4; Sequence=VSP_003939, VSP_003942, VSP_003943;
CC       Name=4;
CC         IsoId=Q9JHH2-5; Sequence=VSP_003939, VSP_003940, VSP_003941;
CC       Name=5;
CC         IsoId=Q9JHH2-6; Sequence=VSP_003939, VSP_003940, VSP_003942;
CC   -!- TISSUE SPECIFICITY: Expressed exclusively in hematopoietic tissues.
CC       Expression detected in spleen, thymus, bone marrow and peripheral blood
CC       leukocytes but not in heart, brain, lung, liver, kidney or testis.
CC       {ECO:0000269|PubMed:10950922}.
CC   -!- DEVELOPMENTAL STAGE: Expressed from early embryogenesis through to
CC       adulthood. {ECO:0000269|PubMed:10950922}.
CC   -!- SIMILARITY: Belongs to the tetraspanin (TM4SF) family. {ECO:0000305}.
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DR   EMBL; AF175771; AAG27268.1; -; mRNA.
DR   EMBL; AJ251788; CAB94716.1; -; Genomic_DNA.
DR   EMBL; AJ251835; CAB94777.1; -; Genomic_DNA.
DR   EMBL; AJ279791; CAB94719.1; -; mRNA.
DR   EMBL; AJ279792; CAB94720.1; -; mRNA.
DR   EMBL; AJ279793; CAB94721.1; -; mRNA.
DR   EMBL; AJ279794; CAB94722.1; -; mRNA.
DR   EMBL; AJ279795; CAB94723.1; -; mRNA.
DR   EMBL; AY039000; AAK84430.1; -; mRNA.
DR   EMBL; AF291425; AAK83110.1; -; mRNA.
DR   CCDS; CCDS52459.1; -. [Q9JHH2-1]
DR   RefSeq; NP_001121552.1; NM_001128080.2. [Q9JHH2-1]
DR   RefSeq; NP_001121553.1; NM_001128081.1.
DR   RefSeq; NP_001121554.1; NM_001128082.1.
DR   RefSeq; NP_064682.1; NM_020286.3.
DR   AlphaFoldDB; Q9JHH2; -.
DR   SMR; Q9JHH2; -.
DR   STRING; 10090.ENSMUSP00000009396; -.
DR   PhosphoSitePlus; Q9JHH2; -.
DR   MaxQB; Q9JHH2; -.
DR   PaxDb; 10090-ENSMUSP00000009396; -.
DR   Antibodypedia; 10387; 205 antibodies from 28 providers.
DR   DNASU; 27027; -.
DR   Ensembl; ENSMUST00000009396.13; ENSMUSP00000009396.7; ENSMUSG00000000244.18. [Q9JHH2-1]
DR   GeneID; 27027; -.
DR   KEGG; mmu:27027; -.
DR   UCSC; uc009kom.2; mouse. [Q9JHH2-1]
DR   AGR; MGI:1350360; -.
DR   CTD; 10077; -.
DR   MGI; MGI:1350360; Tspan32.
DR   VEuPathDB; HostDB:ENSMUSG00000000244; -.
DR   eggNOG; ENOG502S0ED; Eukaryota.
DR   GeneTree; ENSGT00390000003287; -.
DR   HOGENOM; CLU_076116_0_0_1; -.
DR   InParanoid; Q9JHH2; -.
DR   OMA; GQDCLRE; -.
DR   OrthoDB; 4034194at2759; -.
DR   PhylomeDB; Q9JHH2; -.
DR   TreeFam; TF336277; -.
DR   BioGRID-ORCS; 27027; 2 hits in 76 CRISPR screens.
DR   ChiTaRS; Art1; mouse.
DR   PRO; PR:Q9JHH2; -.
DR   Proteomes; UP000000589; Chromosome 7.
DR   RNAct; Q9JHH2; Protein.
DR   Bgee; ENSMUSG00000000244; Expressed in granulocyte and 105 other cell types or tissues.
DR   ExpressionAtlas; Q9JHH2; baseline and differential.
DR   GO; GO:0009986; C:cell surface; IDA:MGI.
DR   GO; GO:0070442; C:integrin alphaIIb-beta3 complex; IDA:MGI.
DR   GO; GO:0007596; P:blood coagulation; IMP:MGI.
DR   GO; GO:0007010; P:cytoskeleton organization; IMP:MGI.
DR   GO; GO:0042832; P:defense response to protozoan; IGI:MGI.
DR   GO; GO:0007599; P:hemostasis; IMP:MGI.
DR   GO; GO:0007229; P:integrin-mediated signaling pathway; IMP:MGI.
DR   GO; GO:0030886; P:negative regulation of myeloid dendritic cell activation; IGI:MGI.
DR   GO; GO:0042130; P:negative regulation of T cell proliferation; IGI:MGI.
DR   GO; GO:0070527; P:platelet aggregation; IMP:MGI.
DR   GO; GO:0050688; P:regulation of defense response to virus; IGI:MGI.
DR   GO; GO:0042098; P:T cell proliferation; IGI:MGI.
DR   CDD; cd03153; PHEMX_like_LEL; 1.
DR   Gene3D; 1.10.1450.10; Tetraspanin; 1.
DR   InterPro; IPR042782; PHEMX_LEL.
DR   InterPro; IPR018499; Tetraspanin/Peripherin.
DR   InterPro; IPR000301; Tetraspanin_animals.
DR   InterPro; IPR008952; Tetraspanin_EC2_sf.
DR   Pfam; PF00335; Tetraspanin; 1.
DR   PIRSF; PIRSF002419; Tetraspanin; 1.
DR   SUPFAM; SSF48652; Tetraspanin; 1.
DR   Genevisible; Q9JHH2; MM.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..256
FT                   /note="Tetraspanin-32"
FT                   /id="PRO_0000219279"
FT   TRANSMEM        15..35
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        61..81
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        90..110
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        203..223
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   VAR_SEQ         1..30
FT                   /note="Missing (in isoform 1, isoform 2, isoform 3, isoform
FT                   4 and isoform 5)"
FT                   /evidence="ECO:0000303|PubMed:10915772,
FT                   ECO:0000303|PubMed:10950922, ECO:0000303|PubMed:11396961"
FT                   /id="VSP_003939"
FT   VAR_SEQ         119..152
FT                   /note="Missing (in isoform 4 and isoform 5)"
FT                   /evidence="ECO:0000303|PubMed:10915772"
FT                   /id="VSP_003940"
FT   VAR_SEQ         182..209
FT                   /note="Missing (in isoform 2 and isoform 4)"
FT                   /evidence="ECO:0000303|PubMed:10915772"
FT                   /id="VSP_003941"
FT   VAR_SEQ         182..206
FT                   /note="DCLQSIRNVLWTHYSIASILTCTSL -> IPWFPDPGTQPLQMDLGWAGASM
FT                   PC (in isoform 3 and isoform 5)"
FT                   /evidence="ECO:0000303|PubMed:10915772"
FT                   /id="VSP_003942"
FT   VAR_SEQ         207..236
FT                   /note="Missing (in isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:10915772"
FT                   /id="VSP_003943"
FT   CONFLICT        174
FT                   /note="Q -> L (in Ref. 1; AAG27268)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        235
FT                   /note="Y -> F (in Ref. 1; AAG27268)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   256 AA;  28906 MW;  A20EF0F6248FF67A CRC64;
     MGHWNRIKIA KCQILITNFL VLLLGLSMAT MVVVIHFGDH FTVIGHASLE RNPYETLRYW
     AFYVGISLAG LLSLGAALST IATVREAHGL MAAGFLCFAL SFCILVQVAF WRFYNPTQVE
     DAVLDTYDFV YDQAMKSPSS NWWQELAVIQ DTFLCCGKKS PFGLLVSTGA IMCQGREAMR
     EDCLQSIRNV LWTHYSIASI LTCTSLALTV YAMMLCAFLW FAIHSYHGLD RKGRYSLTPP
     RSHGFQTQEP SLFRWT
//