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Q9JHH2 (TSN32_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tetraspanin-32

Short name=Tspan-32
Alternative name(s):
AML1-regulated transmembrane protein 1
Protein Phemx
Gene names
Name:Tspan32
Synonyms:Art1, Phemx, Tssc6
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length256 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Subcellular location

Membrane; Multi-pass membrane protein Potential.

Tissue specificity

Expressed exclusively in hematopoietic tissues. Expression detected in spleen, thymus, bone marrow and peripheral blood leukocytes but not in heart, brain, lung, liver, kidney or testis. Ref.1

Developmental stage

Expressed from early embryogenesis through to adulthood. Ref.1

Sequence similarities

Belongs to the tetraspanin (TM4SF) family.

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.
Isoform 6 (identifier: Q9JHH2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 1 (identifier: Q9JHH2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-30: Missing.
Isoform 2 (identifier: Q9JHH2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-30: Missing.
     182-209: Missing.
Isoform 3 (identifier: Q9JHH2-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-30: Missing.
     182-206: DCLQSIRNVLWTHYSIASILTCTSL → IPWFPDPGTQPLQMDLGWAGASMPC
     207-236: Missing.
Isoform 4 (identifier: Q9JHH2-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-30: Missing.
     119-152: Missing.
     182-209: Missing.
Isoform 5 (identifier: Q9JHH2-6)

The sequence of this isoform differs from the canonical sequence as follows:
     1-30: Missing.
     119-152: Missing.
     182-206: DCLQSIRNVLWTHYSIASILTCTSL → IPWFPDPGTQPLQMDLGWAGASMPC

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 256256Tetraspanin-32
PRO_0000219279

Regions

Transmembrane15 – 3521Helical; Potential
Transmembrane61 – 8121Helical; Potential
Transmembrane90 – 11021Helical; Potential
Transmembrane203 – 22321Helical; Potential

Natural variations

Alternative sequence1 – 3030Missing in isoform 1, isoform 2, isoform 3, isoform 4 and isoform 5.
VSP_003939
Alternative sequence119 – 15234Missing in isoform 4 and isoform 5.
VSP_003940
Alternative sequence182 – 20928Missing in isoform 2 and isoform 4.
VSP_003941
Alternative sequence182 – 20625DCLQS…TCTSL → IPWFPDPGTQPLQMDLGWAG ASMPC in isoform 3 and isoform 5.
VSP_003942
Alternative sequence207 – 23630Missing in isoform 3.
VSP_003943

Experimental info

Sequence conflict1741Q → L in AAG27268. Ref.1
Sequence conflict2351Y → F in AAG27268. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 6 [UniParc].

Last modified March 27, 2002. Version 2.
Checksum: A20EF0F6248FF67A

FASTA25628,906
        10         20         30         40         50         60 
MGHWNRIKIA KCQILITNFL VLLLGLSMAT MVVVIHFGDH FTVIGHASLE RNPYETLRYW 

        70         80         90        100        110        120 
AFYVGISLAG LLSLGAALST IATVREAHGL MAAGFLCFAL SFCILVQVAF WRFYNPTQVE 

       130        140        150        160        170        180 
DAVLDTYDFV YDQAMKSPSS NWWQELAVIQ DTFLCCGKKS PFGLLVSTGA IMCQGREAMR 

       190        200        210        220        230        240 
EDCLQSIRNV LWTHYSIASI LTCTSLALTV YAMMLCAFLW FAIHSYHGLD RKGRYSLTPP 

       250 
RSHGFQTQEP SLFRWT 

« Hide

Isoform 1 [UniParc].

Checksum: AD5AE6916BD14BD2
Show »

FASTA22625,525
Isoform 2 [UniParc].

Checksum: 3399ADE104D69B49
Show »

FASTA19822,419
Isoform 3 [UniParc].

Checksum: D7CFC3B0510C7157
Show »

FASTA19621,898
Isoform 4 [UniParc].

Checksum: 23E2CAF9AC04D11F
Show »

FASTA16418,472
Isoform 5 [UniParc].

Checksum: 93092F6EF11DF439
Show »

FASTA19221,455

References

[1]"Phemx, a novel mouse gene expressed in hematopoietic cells maps to the imprinted cluster on distal chromosome 7."
Nicholson R.H., Pantano S., Eliason J.F., Galy A., Weiler S., Kaplan J., Hughes M.R., Ko M.S.H.
Genomics 68:13-21(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Strain: C57BL/6J.
[2]"Sequence conservation and variability of imprinting in the Beckwith-Wiedemann syndrome gene cluster in human and mouse."
Paulsen M., El-Maarri O., Engemann S., Stroedicke M., Franck O., Davies K., Reinhardt R., Reik W., Walter J.
Hum. Mol. Genet. 9:1829-1841(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1; 2; 3; 4 AND 5).
Strain: 129/Sv and C57BL/6.
[3]"Molecular characterisation of mouse and human TSSC6: evidence that TSSC6 is a genuine member of the tetraspanin superfamily and is expressed specifically in haematopoietic organs."
Robb L., Tarrant J., Groom J., Ibrahim M., Li R., Borobakas B., Wright M.D.
Biochim. Biophys. Acta 1522:31-41(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 6).
[4]"A hematopoietic-specific transmembrane protein, Art-1, is possibly regulated by aml1."
Harada Y., Harada H., Downing J.R., Kimura A.
Biochem. Biophys. Res. Commun. 284:714-722(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: C57BL/6.
Tissue: Spleen.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF175771 mRNA. Translation: AAG27268.1.
AJ251788 Genomic DNA. Translation: CAB94716.1.
AJ251835 Genomic DNA. Translation: CAB94777.1.
AJ279791 mRNA. Translation: CAB94719.1.
AJ279792 mRNA. Translation: CAB94720.1.
AJ279793 mRNA. Translation: CAB94721.1.
AJ279794 mRNA. Translation: CAB94722.1.
AJ279795 mRNA. Translation: CAB94723.1.
AY039000 mRNA. Translation: AAK84430.1.
AF291425 mRNA. Translation: AAK83110.1.
RefSeqNP_001121552.1. NM_001128080.2.
NP_001121553.1. NM_001128081.1.
NP_001121554.1. NM_001128082.1.
NP_064682.1. NM_020286.3.
UniGeneMm.28172.

3D structure databases

ProteinModelPortalQ9JHH2.
SMRQ9JHH2. Positions 113-194.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ9JHH2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000009396; ENSMUSP00000009396; ENSMUSG00000000244. [Q9JHH2-1]
GeneID27027.
KEGGmmu:27027.
UCSCuc009kom.2. mouse. [Q9JHH2-1]

Organism-specific databases

CTD10077.
MGIMGI:1350360. Tspan32.

Phylogenomic databases

eggNOGNOG41704.
GeneTreeENSGT00390000003287.
HOVERGENHBG053580.
InParanoidQ9JHH2.
KOK17359.
OMAAARQDCL.
OrthoDBEOG7XDBH0.
PhylomeDBQ9JHH2.
TreeFamTF336277.

Gene expression databases

ArrayExpressQ9JHH2.
BgeeQ9JHH2.
CleanExMM_ART1.
MM_TSPAN32.
GenevestigatorQ9JHH2.

Family and domain databases

InterProIPR000301. Tetraspanin.
IPR018499. Tetraspanin/Peripherin.
IPR008952. Tetraspanin_EC2.
[Graphical view]
PfamPF00335. Tetraspannin. 1 hit.
[Graphical view]
PIRSFPIRSF002419. Tetraspanin. 1 hit.
SUPFAMSSF48652. SSF48652. 1 hit.
ProtoNetSearch...

Other

NextBio304953.
PROQ9JHH2.
SOURCESearch...

Entry information

Entry nameTSN32_MOUSE
AccessionPrimary (citable) accession number: Q9JHH2
Secondary accession number(s): Q923G9 expand/collapse secondary AC list , Q9ESH0, Q9JHQ9, Q9JHR0, Q9JHR1, Q9JHR2, Q9JHS8
Entry history
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: March 27, 2002
Last modified: April 16, 2014
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot