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Protein

Tetraspanin-32

Gene

Tspan32

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

GO - Biological processi

  • blood coagulation Source: MGI
  • cytoskeleton organization Source: MGI
  • defense response to protozoan Source: MGI
  • hemostasis Source: MGI
  • integrin-mediated signaling pathway Source: MGI
  • negative regulation of cell proliferation Source: MGI
  • negative regulation of myeloid dendritic cell activation Source: MGI
  • platelet aggregation Source: MGI
  • regulation of defense response to virus Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Tetraspanin-32
Short name:
Tspan-32
Alternative name(s):
AML1-regulated transmembrane protein 1
Protein Phemx
Gene namesi
Name:Tspan32
Synonyms:Art1, Phemx, Tssc6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1350360. Tspan32.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei15 – 3521HelicalSequence AnalysisAdd
BLAST
Transmembranei61 – 8121HelicalSequence AnalysisAdd
BLAST
Transmembranei90 – 11021HelicalSequence AnalysisAdd
BLAST
Transmembranei203 – 22321HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • cell surface Source: MGI
  • integrin alphaIIb-beta3 complex Source: MGI
  • intracellular Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 256256Tetraspanin-32PRO_0000219279Add
BLAST

Proteomic databases

MaxQBiQ9JHH2.
PRIDEiQ9JHH2.

Expressioni

Tissue specificityi

Expressed exclusively in hematopoietic tissues. Expression detected in spleen, thymus, bone marrow and peripheral blood leukocytes but not in heart, brain, lung, liver, kidney or testis.1 Publication

Developmental stagei

Expressed from early embryogenesis through to adulthood.1 Publication

Gene expression databases

BgeeiQ9JHH2.
CleanExiMM_ART1.
MM_TSPAN32.
ExpressionAtlasiQ9JHH2. baseline and differential.
GenevisibleiQ9JHH2. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000009396.

Structurei

3D structure databases

ProteinModelPortaliQ9JHH2.
SMRiQ9JHH2. Positions 113-194.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tetraspanin (TM4SF) family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG41704.
GeneTreeiENSGT00390000003287.
HOVERGENiHBG053580.
InParanoidiQ9JHH2.
KOiK17359.
OMAiLTYFGAH.
OrthoDBiEOG7XDBH0.
PhylomeDBiQ9JHH2.
TreeFamiTF336277.

Family and domain databases

Gene3Di1.10.1450.10. 1 hit.
InterProiIPR028977. CD81_LEL.
IPR000301. Tetraspanin.
IPR018499. Tetraspanin/Peripherin.
IPR008952. Tetraspanin_EC2.
[Graphical view]
PfamiPF00335. Tetraspannin. 1 hit.
[Graphical view]
PIRSFiPIRSF002419. Tetraspanin. 1 hit.
SUPFAMiSSF48652. SSF48652. 1 hit.

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.

Isoform 6 (identifier: Q9JHH2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGHWNRIKIA KCQILITNFL VLLLGLSMAT MVVVIHFGDH FTVIGHASLE
60 70 80 90 100
RNPYETLRYW AFYVGISLAG LLSLGAALST IATVREAHGL MAAGFLCFAL
110 120 130 140 150
SFCILVQVAF WRFYNPTQVE DAVLDTYDFV YDQAMKSPSS NWWQELAVIQ
160 170 180 190 200
DTFLCCGKKS PFGLLVSTGA IMCQGREAMR EDCLQSIRNV LWTHYSIASI
210 220 230 240 250
LTCTSLALTV YAMMLCAFLW FAIHSYHGLD RKGRYSLTPP RSHGFQTQEP

SLFRWT
Length:256
Mass (Da):28,906
Last modified:March 27, 2002 - v2
Checksum:iA20EF0F6248FF67A
GO
Isoform 1 (identifier: Q9JHH2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-30: Missing.

Show »
Length:226
Mass (Da):25,525
Checksum:iAD5AE6916BD14BD2
GO
Isoform 2 (identifier: Q9JHH2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-30: Missing.
     182-209: Missing.

Show »
Length:198
Mass (Da):22,419
Checksum:i3399ADE104D69B49
GO
Isoform 3 (identifier: Q9JHH2-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-30: Missing.
     182-206: DCLQSIRNVLWTHYSIASILTCTSL → IPWFPDPGTQPLQMDLGWAGASMPC
     207-236: Missing.

Show »
Length:196
Mass (Da):21,898
Checksum:iD7CFC3B0510C7157
GO
Isoform 4 (identifier: Q9JHH2-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-30: Missing.
     119-152: Missing.
     182-209: Missing.

Show »
Length:164
Mass (Da):18,472
Checksum:i23E2CAF9AC04D11F
GO
Isoform 5 (identifier: Q9JHH2-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-30: Missing.
     119-152: Missing.
     182-206: DCLQSIRNVLWTHYSIASILTCTSL → IPWFPDPGTQPLQMDLGWAGASMPC

Show »
Length:192
Mass (Da):21,455
Checksum:i93092F6EF11DF439
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti174 – 1741Q → L in AAG27268 (PubMed:10950922).Curated
Sequence conflicti235 – 2351Y → F in AAG27268 (PubMed:10950922).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 3030Missing in isoform 1, isoform 2, isoform 3, isoform 4 and isoform 5. 3 PublicationsVSP_003939Add
BLAST
Alternative sequencei119 – 15234Missing in isoform 4 and isoform 5. 1 PublicationVSP_003940Add
BLAST
Alternative sequencei182 – 20928Missing in isoform 2 and isoform 4. 1 PublicationVSP_003941Add
BLAST
Alternative sequencei182 – 20625DCLQS…TCTSL → IPWFPDPGTQPLQMDLGWAG ASMPC in isoform 3 and isoform 5. 1 PublicationVSP_003942Add
BLAST
Alternative sequencei207 – 23630Missing in isoform 3. 1 PublicationVSP_003943Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF175771 mRNA. Translation: AAG27268.1.
AJ251788 Genomic DNA. Translation: CAB94716.1.
AJ251835 Genomic DNA. Translation: CAB94777.1.
AJ279791 mRNA. Translation: CAB94719.1.
AJ279792 mRNA. Translation: CAB94720.1.
AJ279793 mRNA. Translation: CAB94721.1.
AJ279794 mRNA. Translation: CAB94722.1.
AJ279795 mRNA. Translation: CAB94723.1.
AY039000 mRNA. Translation: AAK84430.1.
AF291425 mRNA. Translation: AAK83110.1.
CCDSiCCDS52459.1. [Q9JHH2-1]
RefSeqiNP_001121552.1. NM_001128080.2. [Q9JHH2-1]
NP_001121553.1. NM_001128081.1.
NP_001121554.1. NM_001128082.1.
NP_064682.1. NM_020286.3.
UniGeneiMm.28172.

Genome annotation databases

EnsembliENSMUST00000009396; ENSMUSP00000009396; ENSMUSG00000000244. [Q9JHH2-1]
GeneIDi27027.
KEGGimmu:27027.
UCSCiuc009kom.2. mouse. [Q9JHH2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF175771 mRNA. Translation: AAG27268.1.
AJ251788 Genomic DNA. Translation: CAB94716.1.
AJ251835 Genomic DNA. Translation: CAB94777.1.
AJ279791 mRNA. Translation: CAB94719.1.
AJ279792 mRNA. Translation: CAB94720.1.
AJ279793 mRNA. Translation: CAB94721.1.
AJ279794 mRNA. Translation: CAB94722.1.
AJ279795 mRNA. Translation: CAB94723.1.
AY039000 mRNA. Translation: AAK84430.1.
AF291425 mRNA. Translation: AAK83110.1.
CCDSiCCDS52459.1. [Q9JHH2-1]
RefSeqiNP_001121552.1. NM_001128080.2. [Q9JHH2-1]
NP_001121553.1. NM_001128081.1.
NP_001121554.1. NM_001128082.1.
NP_064682.1. NM_020286.3.
UniGeneiMm.28172.

3D structure databases

ProteinModelPortaliQ9JHH2.
SMRiQ9JHH2. Positions 113-194.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000009396.

Proteomic databases

MaxQBiQ9JHH2.
PRIDEiQ9JHH2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000009396; ENSMUSP00000009396; ENSMUSG00000000244. [Q9JHH2-1]
GeneIDi27027.
KEGGimmu:27027.
UCSCiuc009kom.2. mouse. [Q9JHH2-1]

Organism-specific databases

CTDi10077.
MGIiMGI:1350360. Tspan32.

Phylogenomic databases

eggNOGiNOG41704.
GeneTreeiENSGT00390000003287.
HOVERGENiHBG053580.
InParanoidiQ9JHH2.
KOiK17359.
OMAiLTYFGAH.
OrthoDBiEOG7XDBH0.
PhylomeDBiQ9JHH2.
TreeFamiTF336277.

Miscellaneous databases

NextBioi304953.
PROiQ9JHH2.
SOURCEiSearch...

Gene expression databases

BgeeiQ9JHH2.
CleanExiMM_ART1.
MM_TSPAN32.
ExpressionAtlasiQ9JHH2. baseline and differential.
GenevisibleiQ9JHH2. MM.

Family and domain databases

Gene3Di1.10.1450.10. 1 hit.
InterProiIPR028977. CD81_LEL.
IPR000301. Tetraspanin.
IPR018499. Tetraspanin/Peripherin.
IPR008952. Tetraspanin_EC2.
[Graphical view]
PfamiPF00335. Tetraspannin. 1 hit.
[Graphical view]
PIRSFiPIRSF002419. Tetraspanin. 1 hit.
SUPFAMiSSF48652. SSF48652. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Phemx, a novel mouse gene expressed in hematopoietic cells maps to the imprinted cluster on distal chromosome 7."
    Nicholson R.H., Pantano S., Eliason J.F., Galy A., Weiler S., Kaplan J., Hughes M.R., Ko M.S.H.
    Genomics 68:13-21(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: C57BL/6J.
  2. "Sequence conservation and variability of imprinting in the Beckwith-Wiedemann syndrome gene cluster in human and mouse."
    Paulsen M., El-Maarri O., Engemann S., Stroedicke M., Franck O., Davies K., Reinhardt R., Reik W., Walter J.
    Hum. Mol. Genet. 9:1829-1841(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1; 2; 3; 4 AND 5).
    Strain: 129/Sv and C57BL/6.
  3. "Molecular characterisation of mouse and human TSSC6: evidence that TSSC6 is a genuine member of the tetraspanin superfamily and is expressed specifically in haematopoietic organs."
    Robb L., Tarrant J., Groom J., Ibrahim M., Li R., Borobakas B., Wright M.D.
    Biochim. Biophys. Acta 1522:31-41(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 6).
  4. "A hematopoietic-specific transmembrane protein, Art-1, is possibly regulated by aml1."
    Harada Y., Harada H., Downing J.R., Kimura A.
    Biochem. Biophys. Res. Commun. 284:714-722(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: C57BL/6.
    Tissue: Spleen.

Entry informationi

Entry nameiTSN32_MOUSE
AccessioniPrimary (citable) accession number: Q9JHH2
Secondary accession number(s): Q923G9
, Q9ESH0, Q9JHQ9, Q9JHR0, Q9JHR1, Q9JHR2, Q9JHS8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: March 27, 2002
Last modified: July 22, 2015
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.