Reviewed, UniProtKB/Swiss-Prot Q9JHG7 (PK3CG_MOUSE)

Names and origin

Protein names

Recommended name:
    Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma isoform
    EC=2.7.1.153
Alternative name(s):
    PI3-kinase p110 subunit gamma
    PtdIns-3-kinase subunit p110
    PI3Kgamma
      Short name=PI3K
    p120-PI3K

Gene names

Name:	Pik3cg
Synonyms:	Pi3kg1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	1102 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	3-phosphorylates the cellular phosphoinositide PtdIns-4,5-biphosphate (PtdIns(4,5)P2) to produce PtdIns-3, 4,5-triiphosphate (PtdIns(3,4,5)P3). Links G-protein coupled receptor activation to the secondary messenger PtdIns(3,4,5)P3 production.
Catalytic activity	ATP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate.
Enzyme regulation	Activated by both the alpha and the beta-gamma G proteins. Wortmannin sensitive in nM range. Ref.1
Pathway	Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.
Subunit structure	Heterodimer of a catalytic subunit (PIK3CG/p120) and a regulatory (PIK3R5a/p101) subunit By similarity.
Sequence similarities	Belongs to the PI3/PI4-kinase family. Contains 1 PI3K/PI4K domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	negative regulation of apoptosis Inferred from direct assay. Source: MGI phosphoinositide phosphorylation Inferred from electronic annotation. Source: InterPro phosphoinositide-mediated signaling Inferred from electronic annotation. Source: InterPro protein amino acid phosphorylation Inferred from direct assay. Source: MGI
Cellular component	phosphoinositide 3-kinase complex Inferred from electronic annotation. Source: InterPro
Molecular function	1-phosphatidylinositol-3-kinase activity Inferred from electronic annotation. Source: InterPro ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphatidylinositol-4,5-bisphosphate 3-kinase activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Ldha	P06151	2	EBI-644372,EBI-444940

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1102	1102	Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma isoform		PRO_0000088793
Regions
	Domain	828 – 1073	246	PI3K/PI4K
	Compositional bias	18 – 22	5	Poly-Arg

Sequences

	Sequence		Length	Mass (Da)
	Q9JHG7-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: B9E94CFDD0C21D57	FASTA	1,102	126,362
	10 20 30 40 50 60 MELENYEQPV VLREDNLRRR RRMKPRSAAG SLSSMELIPI EFVLPTSQRI SKTPETALLH 70 80 90 100 110 120 VAGHGNVEQM KAQVWLRALE TSVAAEFYHR LGPDQFLLLY QKKGQWYEIY DRYQVVQTLD 130 140 150 160 170 180 CLHYWKLMHK SPGQIHVVQR HVPSEETLAF QKQLTSLIGY DVTDISNVHD DELEFTRRRL 190 200 210 220 230 240 VTPRMAEVAG RDAKLYAMHP WVTSKPLPDY LSKKIANNCI FIVIHRGTTS QTIKVSADDT 250 260 270 280 290 300 PGTILQSFFT KMAKKKSLMN IPESQSEQDF VLRVCGRDEY LVGETPLKNF QWVRQCLKNG 310 320 330 340 350 360 DEIHLVLDTP PDPALDEVRK EEWPLVDDCT GVTGYHEQLT IHGKDHESVV TVSLWDCDRK 370 380 390 400 410 420 FRVKIRGIDI PVLPRNTDLT VFVEANIQHG QQVLCQRRTS PKPFAEEVLW NVWLEFGIKI 430 440 450 460 470 480 KDLPKGALLN LQIYCCKTPS LSSKASAETP GSESKGKAQL LYYVNLLLID HRFLLRHGDY 490 500 510 520 530 540 VLHMWQISGK AEEQGSFNAD KLTSATNPDK ENSMSISILL DNYCHPIALP KHRPTPDPEG 550 560 570 580 590 600 DRVRAEMPNQ LRKQLEAIIA TDPLNPLTAE DKELLWHFRY ESLKHPKAYP KLFSSVKWGQ 610 620 630 640 650 660 QEIVAKTYQL LARREIWDQS ALDVGLTMQL LDCNFSDENV RAIAVQKLES LEDDDVLHYL 670 680 690 700 710 720 LQLVQAVKFE PYHDSALARF LLKRGLRNKR IGHFLFWFLR SEIAQSRHYQ QRFAVILEAY 730 740 750 760 770 780 LRGCGTAMLQ DFTQQVHVIE MLQKVTIDIK SLSAEKYDVS SQVISQLKQK LESLQNSNLP 790 800 810 820 830 840 ESFRVPYDPG LKAGTLVIEK CKVMASKKKP LWLEFKCADP TVLSNETIGI IFKHGDDLRQ 850 860 870 880 890 900 DMLILQILRI MESIWETESL DLCLLPYGCI STGDKIGMIE IVKDATTIAQ IQQSTVGNTG 910 920 930 940 950 960 AFKDEVLNHW LKEKCPIEEK FQAAVERFVY SCAGYCVATF VLGIGDRHND NIMISETGNL 970 980 990 1000 1010 1020 FHIDFGHILG NYKSFLGINK ERVPFVLTPD FLFVMGSSGK KTSPHFQKFQ DVCVRAYLAL 1030 1040 1050 1060 1070 1080 RHHTNLLIIL FSMMLMTGMP QLTSKEDIEY IRDALTVGKS EEDAKKYFLD QIEVCRDKGW 1090 1100 TVQFNWFLHL VLGIKQGEKH SA

References

[1]	"Analysis of the murine phosphoinositide 3-kinase gamma gene." Hirsch E., Wymann M.P., Patrucco E., Tolosano E., Bulgarelli-Leva G., Marengo S., Rocchi M., Altruda F. Gene 256:69-81(2000) [PubMed: 11054537] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], ENZYME REGULATION.
[2]	Lubec G., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 1060-1065, MASS SPECTROMETRY. Strain: OF1. Tissue: Hippocampus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ249413 , AJ249414, AJ249415, AJ249416, AJ249417, AJ249418, AJ249419, AJ249420 Genomic DNA. Translation: CAB89851.1. AJ249280 mRNA. Translation: CAB89686.1.
IPI	IPI00623682.
UniGene	Mm.101369 Mm.404021
3D structure databases
HSSP	HSSP built from PDB template 1E7U based on UniProtKB O02697.
SMR	Q9JHG7. Positions 144-1094.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9JHG7. 2 interactions.
STRING	Q9JHG7.
PTM databases
PhosphoSite	Q9JHG7.
Proteomic databases
PRIDE	Q9JHG7.
Genome annotation databases
Ensembl	ENSMUST00000020890; ENSMUSP00000020890; ENSMUSG00000020573; Mus musculus. [Genome view] ENSMUST00000053215; ENSMUSP00000062864; ENSMUSG00000020573; Mus musculus. [Genome view] ENSMUST00000085469; ENSMUSP00000082596; ENSMUSG00000020573; Mus musculus. [Genome view]
UCSC	uc007nid.1. mouse.
Organism-specific databases
MGI	MGI:1353576. Pik3cg.
Phylogenomic databases
HOVERGEN	Q9JHG7.
Enzyme and pathway databases
BRENDA	2.7.1.153. 244.
Gene expression databases
ArrayExpress	Q9JHG7.
Bgee	Q9JHG7.
Genevestigator	Q9JHG7.
GermOnline	ENSMUSG00000020573. Mus musculus.
Family and domain databases
InterPro	IPR000403. PI3/4_kinase_cat. IPR018936. PI3/4_kinase_CS. IPR002420. PI3K_C2. IPR000341. PI3K_ras_bd. IPR001263. PI3Ka. IPR015433. PI_Kinase. [Graphical view]
Gene3D	G3DSA:1.10.1070.11. PI3/4_kinase_cat. 1 hit. G3DSA:1.25.40.70. PI3Ka. 1 hit.
PANTHER	PTHR10048. PI_Kinase. 1 hit.
Pfam	PF00454. PI3_PI4_kinase. 1 hit. PF00792. PI3K_C2. 1 hit. PF00794. PI3K_rbd. 1 hit. PF00613. PI3Ka. 1 hit. [Graphical view]
SMART	SM00142. PI3K_C2. 1 hit. SM00144. PI3K_rbd. 1 hit. SM00145. PI3Ka. 1 hit. SM00146. PI3Kc. 1 hit. [Graphical view]
PROSITE	PS00915. PI3_4_KINASE_1. 1 hit. PS00916. PI3_4_KINASE_2. 1 hit. PS50290. PI3_4_KINASE_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	307436.
SOURCE	Search...

Entry information

Entry name

PK3CG_MOUSE

Accession

Primary (citable) accession number: Q9JHG7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	October 1, 2000
Last modified:	October 13, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)