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Protein

Apelin receptor

Gene

Aplnr

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for apelin coupled to G proteins that inhibit adenylate cyclase activity and plays a role in various processes in adults such as regulation of blood pressure, heart contractility, and heart failure. Also plays a key role in early development such as gastrulation and heart morphogenesis by acting as a receptor for APELA hormone.

GO - Molecular functioni

  • G-protein coupled receptor activity Source: RGD
  • peptide binding Source: RGD

GO - Biological processi

  • gastrulation Source: UniProtKB-KW
  • G-protein coupled receptor signaling pathway Source: RGD
  • heart development Source: UniProtKB
  • regulation of body fluid levels Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Gastrulation

Enzyme and pathway databases

ReactomeiR-RNO-375276. Peptide ligand-binding receptors.
R-RNO-418594. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Apelin receptor
Alternative name(s):
Angiotensin receptor-like 1
B78
G-protein coupled receptor APJ
GPCR34
Gene namesi
Name:Aplnr
Synonyms:Agtrl1, Apj
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi621645. Aplnr.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3030ExtracellularSequence analysisAdd
BLAST
Transmembranei31 – 5323Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini54 – 6411CytoplasmicSequence analysisAdd
BLAST
Transmembranei65 – 8723Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini88 – 10619ExtracellularSequence analysisAdd
BLAST
Transmembranei107 – 12923Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini130 – 14112CytoplasmicSequence analysisAdd
BLAST
Transmembranei142 – 16423Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini165 – 20036ExtracellularSequence analysisAdd
BLAST
Transmembranei201 – 22323Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini224 – 24219CytoplasmicSequence analysisAdd
BLAST
Transmembranei243 – 26523Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini266 – 28419ExtracellularSequence analysisAdd
BLAST
Transmembranei285 – 30723Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini308 – 37770CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: RGD
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 377377Apelin receptorPRO_0000069176Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi13 – 131N-linked (GlcNAc...)Sequence analysis
Glycosylationi173 – 1731N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9JHG3.

PTM databases

PhosphoSiteiQ9JHG3.

Expressioni

Tissue specificityi

Widely expressed. Highest expression in the lung, lower in the heart, placenta, ovary, skeletal muscle, mammary gland, kidney and several structures in the brain as the hypothalamus (supraoptic and periventricular nuclei), pituitary, olfactory bulb and pineal gland.

Developmental stagei

Higher expression in neonates than in adult rats.

Gene expression databases

GenevisibleiQ9JHG3. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000012379.

Structurei

3D structure databases

ProteinModelPortaliQ9JHG3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000119055.
HOGENOMiHOG000234122.
HOVERGENiHBG104998.
InParanoidiQ9JHG3.
KOiK04174.
OMAiVMVFRST.
OrthoDBiEOG7Z0JWM.
PhylomeDBiQ9JHG3.
TreeFamiTF330024.

Family and domain databases

InterProiIPR020279. Apelin_receptor_C.
IPR003904. APJ_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01416. APJRECEPTOR.
PR00237. GPCRRHODOPSN.
ProDomiPD061381. Apelin_receptor_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JHG3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDDGYNYYG ADNQSECDYA DWTPSGALIP AIYILVFLLG TTGNGLVLWT
60 70 80 90 100
VFWSSREKRR SADIFIASLA VADLTFVVTL PLWATYTYRE FDWPFGTFSC
110 120 130 140 150
KLSSYLIFVN MYASVFCLTG LSFDRYLAIV RPVANARLRL RVSGAVATAV
160 170 180 190 200
LWVLAALLAV PVMVFRSTDI PENSTKTQCY MDYSMVATSN SEWAWEVGLG
210 220 230 240 250
VSSTAVGFVV PFIIMLTCYF FIAQTIAGHF RKERIEGLRK RRRLLSIIVV
260 270 280 290 300
LVVTFALCWM PYHLVKTLYM LGNLLHWPCD FDSFLMNVFP YCTCISYVNS
310 320 330 340 350
CLNPFLYAFF DPRFRRACTS MLCCDQSGCK GSPHSSSAEK SASYSSGHSQ
360 370
GPGPNMCKGG EPMHEKSIPY SQETLVD
Length:377
Mass (Da):42,349
Last modified:October 1, 2000 - v1
Checksum:iC43F81EBC3855BB2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti117 – 1171C → W in AAG24468 (Ref. 5) Curated
Sequence conflicti187 – 1871A → G in AAG24468 (Ref. 5) Curated
Sequence conflicti198 – 1981G → R in AAG24468 (Ref. 5) Curated
Sequence conflicti251 – 2511L → P in AAG24468 (Ref. 5) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB033170 mRNA. Translation: BAA95002.1.
AF184883 mRNA. Translation: AAF80860.1.
BC072494 mRNA. Translation: AAH72494.1.
AF090346 mRNA. Translation: AAG24468.1.
RefSeqiNP_112639.1. NM_031349.2.
UniGeneiRn.7965.

Genome annotation databases

EnsembliENSRNOT00000012379; ENSRNOP00000012379; ENSRNOG00000009227.
GeneIDi83518.
KEGGirno:83518.
UCSCiRGD:621645. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB033170 mRNA. Translation: BAA95002.1.
AF184883 mRNA. Translation: AAF80860.1.
BC072494 mRNA. Translation: AAH72494.1.
AF090346 mRNA. Translation: AAG24468.1.
RefSeqiNP_112639.1. NM_031349.2.
UniGeneiRn.7965.

3D structure databases

ProteinModelPortaliQ9JHG3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000012379.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiQ9JHG3.

Proteomic databases

PaxDbiQ9JHG3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000012379; ENSRNOP00000012379; ENSRNOG00000009227.
GeneIDi83518.
KEGGirno:83518.
UCSCiRGD:621645. rat.

Organism-specific databases

CTDi187.
RGDi621645. Aplnr.

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000119055.
HOGENOMiHOG000234122.
HOVERGENiHBG104998.
InParanoidiQ9JHG3.
KOiK04174.
OMAiVMVFRST.
OrthoDBiEOG7Z0JWM.
PhylomeDBiQ9JHG3.
TreeFamiTF330024.

Enzyme and pathway databases

ReactomeiR-RNO-375276. Peptide ligand-binding receptors.
R-RNO-418594. G alpha (i) signalling events.

Miscellaneous databases

PROiQ9JHG3.

Gene expression databases

GenevisibleiQ9JHG3. RN.

Family and domain databases

InterProiIPR020279. Apelin_receptor_C.
IPR003904. APJ_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01416. APJRECEPTOR.
PR00237. GPCRRHODOPSN.
ProDomiPD061381. Apelin_receptor_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular and functional characteristics of APJ: tissue distribution of mRNA and interaction with the endogenous ligand apelin."
    Hosoya M., Kawamata Y., Fukusumi S., Fujii R., Habata Y., Hinuma S., Kitada C., Honda S., Kurokawa T., Onda H., Nishimura O., Fujino M.
    J. Biol. Chem. 275:21061-21067(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Distribution of mRNA encoding B78/apj, the rat homologue of the human APJ receptor, and its endogenous ligand apelin in brain and peripheral tissues."
    O'Carroll A.-M., Selby T.L., Palkovits M., Lolait S.J.
    Biochim. Biophys. Acta 1492:72-80(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Pituitary.
  3. "Cloning, pharmacological characterization and brain distribution of the rat apelin receptor."
    De Mota N., Lenkei Z., Llorens-Cortes C.
    Neuroendocrinology 72:400-407(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  5. "Identification and characterization of novel G-protein coupled receptors expressed in regenerating peripheral nerve."
    Carroll S.L., Miller M.L., Benedict-Hamilton H.M.
    Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 75-300.
    Strain: Sprague-Dawley.
  6. "Physiological role of a novel neuropeptide, apelin, and its receptor in the rat brain."
    Reaux A., De Mota N., Skultetyova I., Lenkei Z., El Messari S., Gallatz K., Corvol P., Palkovits M., Llorens-Cortes C.
    J. Neurochem. 77:1085-1096(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiAPJ_RAT
AccessioniPrimary (citable) accession number: Q9JHG3
Secondary accession number(s): Q9ESK2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: October 1, 2000
Last modified: June 8, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.