Q9JHF9 (LY96_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lymphocyte antigen 96
Short name=Ly-96

Alternative name(s):
ESOP-1
Protein MD-2

Gene names

Name:	Ly96
Synonyms:	Esop1, Md2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	160 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cooperates with TLR4 in the innate immune response to bacterial lipopolysaccharide (LPS), and with TLR2 in the response to cell wall components from Gram-positive and Gram-negative bacteria. Enhances TLR4-dependent activation of NF-kappa-B. Cells expressing both MD2 and TLR4, but not TLR4 alone, respond to LPS By similarity.
Subunit structure	Heterogeneous homopolymer formed from homodimers; disulfide-linked. Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, LY96 and TLR4. Binds to the extracellular domains of TLR2 and TLR4. Ligand binding induces interaction with TLR4 and oligomerization of the complex By similarity. Ref.4
Subcellular location	Secreted › extracellular space.
Tissue specificity	Highly expressed in spleen, bone marrow, thymus, liver, kidney, ovary and decidua. Detected at lower levels in testis, small intestine and skin.

Ontologies

Keywords
Biological process	Immunity Inflammatory response Innate immunity
Cellular component	Secreted
Domain	Signal
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	detection of lipopolysaccharide Inferred from sequence or structural similarity. Source: UniProtKB inflammatory response Inferred from electronic annotation. Source: UniProtKB-KW innate immune response Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of lipopolysaccharide-mediated signaling pathway Inferred from direct assay PubMed 10880523. Source: MGI
Cellular_component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell lipopolysaccharide receptor complex Inferred from electronic annotation. Source: Compara
Molecular_function	lipopolysaccharide receptor activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Tlr4	Q9QUK6	3	EBI-1534566,EBI-1534575

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 18

18

Potential

Chain

19 – 160

142

Lymphocyte antigen 96

PRO_0000018620

Amino acid modifications

Glycosylation

26

1

N-linked (GlcNAc...) Ref.4

Glycosylation

114

1

N-linked (GlcNAc...) Ref.4

Glycosylation

150

1

N-linked (GlcNAc...) Ref.4

Disulfide bond

25 ↔ 51

Ref.4

Disulfide bond

37 ↔ 148

Ref.4

Disulfide bond

95 ↔ 105

Ref.4

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

160

Helix Strand Turn

Details...

Beta strand

23 – 26

4

Beta strand

31 – 37

7

Beta strand

44 – 50

7

Beta strand

57 – 65

9

Beta strand

74 – 82

9

Beta strand

90 – 94

5

Beta strand

97 – 99

3

Helix

103 – 106

4

Beta strand

113 – 121

9

Beta strand

128 – 139

12

Turn

140 – 142

3

Beta strand

145 – 154

10

Sequences

	Sequence		Length	Mass (Da)
	Q9JHF9 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: E224D17E5D5429E2	FASTA	160	18,394
	10 20 30 40 50 60 MLPFILFSTL LSPILTESEK QQWFCNSSDA IISYSYCDHL KFPISISSEP CIRLRGTNGF 70 80 90 100 110 120 VHVEFIPRGN LKYLYFNLFI SVNSIELPKR KEVLCHGHDD DYSFCRALKG ETVNTSIPFS 130 140 150 160 FEGILFPKGH YRCVAEAIAG DTEEKLFCLN FTIIHRRDVN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"ESOP-1, a secreted protein expressed in the hematopoietic, nervous, and reproductive systems of embryonic and adult mice." Kato K., Morrison A.M., Nakano T., Tashiro K., Honjo T. Blood 96:362-364(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cell surface expression and lipopolysaccaride signaling via the Toll-like receptor 4-MD-2 complex on mouse peritoneal macrophages." Akashi S., Shimazu R., Ogata H., Nagai Y., Takeda K., Kimoto M., Miyake K. J. Immunol. 164:3471-3475(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Embryo.
[4]	"Crystal structure of the TLR4-MD-2 complex with bound endotoxin antagonist Eritoran." Kim H.M., Park B.S., Kim J.-I., Kim S.E., Lee J., Oh S.C., Enkhbayar P., Matsushima N., Lee H., Yoo O.J., Lee J.-O. Cell 130:906-917(2007) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.84 ANGSTROMS) OF 21-155 IN COMPLEX WITH TLR4, GLYCOSYLATION AT ASN-26; ASN-114 AND ASN-150, DISULFIDE BONDS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF168120 mRNA. Translation: AAF89634.1.
AB018550 mRNA. Translation: BAA93619.1.
AK019283 mRNA. Translation: BAB31645.1.

IPI

IPI00119324.

RefSeq

NP_001153183.1. NM_001159711.1.
NP_058619.1. NM_016923.2.

UniGene

Mm.116844.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2Z64	X-ray	2.84	C	21-155	[»]
3VQ1	X-ray	2.70	C/D	17-160	[»]
3VQ2	X-ray	2.48	C/D	17-160	[»]

ProteinModelPortal

Q9JHF9.

SMR

Q9JHF9. Positions 21-155.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-38572N.

IntAct

Q9JHF9. 1 interaction.

PTM databases

PhosphoSite

Q9JHF9.

Proteomic databases

PRIDE

Q9JHF9.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000026881; ENSMUSP00000026881; ENSMUSG00000025779.

GeneID

17087.

KEGG

mmu:17087.

UCSC

uc007ajz.2. mouse.

Organism-specific databases

CTD

23643.

MGI

MGI:1341909. Ly96.

Phylogenomic databases

eggNOG

NOG46291.

GeneTree

ENSGT00390000000742.

HOGENOM

HOG000001152.

HOVERGEN

HBG032514.

InParanoid

Q9JHF9.

KO

K05400.

OMA

YIPRRDI.

OrthoDB

EOG4VQ9QM.

Gene expression databases

ArrayExpress

Q9JHF9.

Bgee

Q9JHF9.

CleanEx

MM_LY96.

Genevestigator

Q9JHF9.

GermOnline

ENSMUSG00000025779. Mus musculus.

Family and domain databases

Gene3D

2.60.40.770. 1 hit.

InterPro

IPR014756. Ig_E-set.
IPR003172. MD-2_lipid-recog_dom.
[Graphical view]

Pfam

PF02221. E1_DerP2_DerF2. 1 hit.
[Graphical view]

SMART

SM00737. ML. 1 hit.
[Graphical view]

SUPFAM

SSF81296. Ig_E-set. 1 hit.

ProtoNet

Search...

Other

ChiTaRS

LY96. mouse.

EvolutionaryTrace

Q9JHF9.

NextBio

291228.

SOURCE

Search...

Entry information

Entry name

LY96_MOUSE

Accession

Primary (citable) accession number: Q9JHF9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	October 1, 2000
Last modified:	May 29, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program