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Protein

Transcription cofactor HES-6

Gene

Hes6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Does not bind DNA itself but suppresses both HES1-mediated N box-dependent transcriptional repression and binding of HES1 to E box sequences. Also suppresses HES1-mediated inhibition of the heterodimer formed by ASCL1/MASH1 and TCF3/E47, allowing ASCL1 and TCF3 to up-regulate transcription in its presence. Promotes cell differentiation.1 Publication

GO - Molecular functioni

  • RNA polymerase II regulatory region sequence-specific DNA binding Source: NTNU_SB
  • RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: NTNU_SB
  • sequence-specific DNA binding transcription factor activity Source: MGI
  • transcription cofactor activity Source: MGI
  • transcription factor binding Source: UniProtKB

GO - Biological processi

  • cell differentiation Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
  • nervous system development Source: MGI
  • regulation of transcription from RNA polymerase II promoter Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Differentiation, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription cofactor HES-6
Alternative name(s):
Hairy and enhancer of split 6
Gene namesi
Name:Hes6Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1859852. Hes6.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: UniProtKB-SubCell
  • transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 224224Transcription cofactor HES-6PRO_0000127215Add
BLAST

Proteomic databases

PRIDEiQ9JHE6.

PTM databases

PhosphoSiteiQ9JHE6.

Expressioni

Tissue specificityi

Expressed in both undifferentiated and differentiated cells. High levels of expression are observed in several embryonic tissues including the nervous system, muscle and thymus. In the nervous system, initially expressed in the closing neural tube, then in the spinal cord, cranial and dorsal root ganglia, and brain neuroepithelium. Also expressed in epithelial cells of the embryonic respiratory, urinary and digestive systems. In the limb buds, expressed in skeletal muscle and presumptive tendons.2 Publications

Developmental stagei

Expressed in both embryo and adult. Expression in the embryo is detected from E8.5. In the retina, expressed at high levels at postnatal E0. Expression in brain and retina decreases postnatally but still detectable in adult.1 Publication

Gene expression databases

BgeeiQ9JHE6.
CleanExiMM_HES6.
GenevestigatoriQ9JHE6.

Interactioni

Subunit structurei

Transcription repression requires formation of a complex with a corepressor protein of the Groucho/TLE family (By similarity). Interacts with HES1.By similarity1 Publication

Protein-protein interaction databases

BioGridi207731. 5 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ9JHE6.
SMRiQ9JHE6. Positions 26-81.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 7753bHLHPROSITE-ProRule annotationAdd
BLAST
Domaini96 – 12934OrangePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi221 – 2244WRPW motif

Domaini

The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.By similarity
Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation
Contains 1 Orange domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG241578.
GeneTreeiENSGT00700000104168.
HOGENOMiHOG000236346.
HOVERGENiHBG107098.
InParanoidiQ9JHE6.
KOiK09087.
OMAiEDEDRWE.
OrthoDBiEOG7GBFXZ.
PhylomeDBiQ9JHE6.
TreeFamiTF351373.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR003650. Orange.
[Graphical view]
PfamiPF07527. Hairy_orange. 1 hit.
PF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
PS51054. ORANGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JHE6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPSQAPSRD RAGQEDEDRW EARGDRKARK PLVEKKRRAR INESLQELRL
60 70 80 90 100
LLAGTEVQAK LENAEVLELT VRRVQGALRG RAREREQLQA EASERFAAGY
110 120 130 140 150
IQCMHEVHTF VSTCQAIDAT VSAELLNHLL ESMPLREGSS FQDLLGDSLA
160 170 180 190 200
GLPGGSGRSS WPPGGSPESP LSSPPGPGDD LCSDLEEIPE AELNRVPAEG
210 220
PDLVSTSLGS LTAARRAQSV WRPW
Length:224
Mass (Da):24,454
Last modified:October 1, 2000 - v1
Checksum:iF5160A69D913DBE2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB035178 mRNA. Translation: BAA96081.1.
AF247040 mRNA. Translation: AAF63757.1.
AF260236 mRNA. Translation: AAK51633.1.
AK013443 mRNA. Translation: BAB28856.1.
AK076135 mRNA. Translation: BAC36210.1.
AK155340 mRNA. Translation: BAE33204.1.
BC012897 mRNA. Translation: AAH12897.1.
CCDSiCCDS15163.1.
RefSeqiNP_062352.1. NM_019479.3.
UniGeneiMm.280029.

Genome annotation databases

EnsembliENSMUST00000086851; ENSMUSP00000084062; ENSMUSG00000067071.
GeneIDi55927.
KEGGimmu:55927.
UCSCiuc007cau.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB035178 mRNA. Translation: BAA96081.1.
AF247040 mRNA. Translation: AAF63757.1.
AF260236 mRNA. Translation: AAK51633.1.
AK013443 mRNA. Translation: BAB28856.1.
AK076135 mRNA. Translation: BAC36210.1.
AK155340 mRNA. Translation: BAE33204.1.
BC012897 mRNA. Translation: AAH12897.1.
CCDSiCCDS15163.1.
RefSeqiNP_062352.1. NM_019479.3.
UniGeneiMm.280029.

3D structure databases

ProteinModelPortaliQ9JHE6.
SMRiQ9JHE6. Positions 26-81.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207731. 5 interactions.

PTM databases

PhosphoSiteiQ9JHE6.

Proteomic databases

PRIDEiQ9JHE6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000086851; ENSMUSP00000084062; ENSMUSG00000067071.
GeneIDi55927.
KEGGimmu:55927.
UCSCiuc007cau.1. mouse.

Organism-specific databases

CTDi55502.
MGIiMGI:1859852. Hes6.

Phylogenomic databases

eggNOGiNOG241578.
GeneTreeiENSGT00700000104168.
HOGENOMiHOG000236346.
HOVERGENiHBG107098.
InParanoidiQ9JHE6.
KOiK09087.
OMAiEDEDRWE.
OrthoDBiEOG7GBFXZ.
PhylomeDBiQ9JHE6.
TreeFamiTF351373.

Miscellaneous databases

ChiTaRSiHes6. mouse.
NextBioi311596.
PROiQ9JHE6.
SOURCEiSearch...

Gene expression databases

BgeeiQ9JHE6.
CleanExiMM_HES6.
GenevestigatoriQ9JHE6.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR003650. Orange.
[Graphical view]
PfamiPF07527. Hairy_orange. 1 hit.
PF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
PS51054. ORANGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The bHLH gene Hes6, an inhibitor of Hes1, promotes neuronal differentiation."
    Bae S.-K., Bessho Y., Hojo M., Kageyama R.
    Development 127:2933-2943(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH HES1, DEVELOPMENTAL STAGE.
    Tissue: Embryo1 Publication.
  2. "Expression of hes6, a new member of the Hairy/Enhancer-of-split family, in mouse development."
    Pissarra L., Henrique D., Duarte A.
    Mech. Dev. 95:275-278(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
  3. "Expression of mouse HES-6, a new member of the Hairy/Enhancer of split family of bHLH transcription factors."
    Vasiliauskas D., Stern C.D.
    Mech. Dev. 98:133-137(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: C57BL/6JImported.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Embryo.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech IIImported.
    Tissue: Mammary tumor.

Entry informationi

Entry nameiHES6_MOUSE
AccessioniPrimary (citable) accession number: Q9JHE6
Secondary accession number(s): Q3U2D7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: October 1, 2000
Last modified: February 4, 2015
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.