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Q9JHE6 (HES6_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription cofactor HES-6
Alternative name(s):
Hairy and enhancer of split 6
Gene names
Name:Hes6
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length224 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Does not bind DNA itself but suppresses both HES1-mediated N box-dependent transcriptional repression and binding of HES1 to E box sequences. Also suppresses HES1-mediated inhibition of the heterodimer formed by ASCL1/MASH1 and TCF3/E47, allowing ASCL1 and TCF3 to up-regulate transcription in its presence. Promotes cell differentiation. Ref.1

Subunit structure

Transcription repression requires formation of a complex with a corepressor protein of the Groucho/TLE family By similarity. Interacts with HES1. Ref.1

Subcellular location

Nucleus Probable.

Tissue specificity

Expressed in both undifferentiated and differentiated cells. High levels of expression are observed in several embryonic tissues including the nervous system, muscle and thymus. In the nervous system, initially expressed in the closing neural tube, then in the spinal cord, cranial and dorsal root ganglia, and brain neuroepithelium. Also expressed in epithelial cells of the embryonic respiratory, urinary and digestive systems. In the limb buds, expressed in skeletal muscle and presumptive tendons. Ref.2 Ref.3

Developmental stage

Expressed in both embryo and adult. Expression in the embryo is detected from E8.5. In the retina, expressed at high levels at postnatal E0. Expression in brain and retina decreases postnatally but still detectable in adult. Ref.1

Domain

The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins By similarity. UniProtKB Q26263

Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).

Sequence similarities

Contains 1 bHLH (basic helix-loop-helix) domain.

Contains 1 Orange domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 224224Transcription cofactor HES-6
PRO_0000127215

Regions

Domain25 – 7753bHLH
Domain96 – 12934Orange
Motif221 – 2244WRPW motif

Sequences

Sequence LengthMass (Da)Tools
Q9JHE6 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: F5160A69D913DBE2

FASTA22424,454
        10         20         30         40         50         60 
MAPSQAPSRD RAGQEDEDRW EARGDRKARK PLVEKKRRAR INESLQELRL LLAGTEVQAK 

        70         80         90        100        110        120 
LENAEVLELT VRRVQGALRG RAREREQLQA EASERFAAGY IQCMHEVHTF VSTCQAIDAT 

       130        140        150        160        170        180 
VSAELLNHLL ESMPLREGSS FQDLLGDSLA GLPGGSGRSS WPPGGSPESP LSSPPGPGDD 

       190        200        210        220 
LCSDLEEIPE AELNRVPAEG PDLVSTSLGS LTAARRAQSV WRPW 

« Hide

References

« Hide 'large scale' references
[1]"The bHLH gene Hes6, an inhibitor of Hes1, promotes neuronal differentiation."
Bae S.-K., Bessho Y., Hojo M., Kageyama R.
Development 127:2933-2943(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH HES1, DEVELOPMENTAL STAGE.
Tissue: Embryo.
[2]"Expression of hes6, a new member of the Hairy/Enhancer-of-split family, in mouse development."
Pissarra L., Henrique D., Duarte A.
Mech. Dev. 95:275-278(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[3]"Expression of mouse HES-6, a new member of the Hairy/Enhancer of split family of bHLH transcription factors."
Vasiliauskas D., Stern C.D.
Mech. Dev. 98:133-137(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: C57BL/6J.
[4]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J and NOD.
Tissue: Embryo.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Czech II.
Tissue: Mammary tumor.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB035178 mRNA. Translation: BAA96081.1.
AF247040 mRNA. Translation: AAF63757.1.
AF260236 mRNA. Translation: AAK51633.1.
AK013443 mRNA. Translation: BAB28856.1.
AK076135 mRNA. Translation: BAC36210.1.
AK155340 mRNA. Translation: BAE33204.1.
BC012897 mRNA. Translation: AAH12897.1.
CCDSCCDS15163.1.
RefSeqNP_062352.1. NM_019479.3.
UniGeneMm.280029.

3D structure databases

ProteinModelPortalQ9JHE6.
SMRQ9JHE6. Positions 26-81.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid207731. 5 interactions.

PTM databases

PhosphoSiteQ9JHE6.

Proteomic databases

PRIDEQ9JHE6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000086851; ENSMUSP00000084062; ENSMUSG00000067071.
GeneID55927.
KEGGmmu:55927.
UCSCuc007cau.1. mouse.

Organism-specific databases

CTD55502.
MGIMGI:1859852. Hes6.

Phylogenomic databases

eggNOGNOG241578.
GeneTreeENSGT00700000104168.
HOGENOMHOG000236346.
HOVERGENHBG107098.
InParanoidQ9JHE6.
KOK09087.
OMAREDEDGC.
OrthoDBEOG7GBFXZ.
PhylomeDBQ9JHE6.
TreeFamTF351373.

Gene expression databases

BgeeQ9JHE6.
CleanExMM_HES6.
GenevestigatorQ9JHE6.

Family and domain databases

Gene3D4.10.280.10. 1 hit.
InterProIPR011598. bHLH_dom.
IPR003650. Orange.
[Graphical view]
PfamPF07527. Hairy_orange. 1 hit.
PF00010. HLH. 1 hit.
[Graphical view]
SMARTSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMSSF47459. SSF47459. 1 hit.
PROSITEPS50888. BHLH. 1 hit.
PS51054. ORANGE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSHES6. mouse.
NextBio311596.
PROQ9JHE6.
SOURCESearch...

Entry information

Entry nameHES6_MOUSE
AccessionPrimary (citable) accession number: Q9JHE6
Secondary accession number(s): Q3U2D7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: October 1, 2000
Last modified: July 9, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot