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Protein

Galactosylceramide sulfotransferase

Gene

Gal3st1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the sulfation of membrane glycolipids. Seems to prefer beta-glycosides at the non-reducing termini of sugar chains attached to a lipid moiety. Catalyzes the synthesis of HSO3-3-galactosylceramide (sulfatide), a major lipid component of the myelin sheath and of HSO3-3-monogalactosylalkylacylglycerol (seminolipid), present in spermatocytes. Also acts on lactosylceramide, galactosyl 1-alkyl-2-sn-glycerol and galactosyl diacylglycerol (in vitro).1 Publication

Catalytic activityi

3'-phosphoadenylyl sulfate + a galactosylceramide = adenosine 3',5'-bisphosphate + a galactosylceramidesulfate.
3'-phosphoadenylyl sulfate + monogalactosylalkylacylglycerol = adenosine 3',5'-bisphosphate + monogalactosylalkylacylglycerol sulfate.

Pathwayi: sphingolipid metabolism

This protein is involved in the pathway sphingolipid metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway sphingolipid metabolism and in Lipid metabolism.

GO - Molecular functioni

  • galactosylceramide sulfotransferase activity Source: MGI

GO - Biological processi

  • galactosylceramide biosynthetic process Source: MGI
  • glycolipid biosynthetic process Source: MGI
  • myelination Source: MGI
  • spermatogenesis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Lipid metabolism, Sphingolipid metabolism

Enzyme and pathway databases

BRENDAi2.8.2.11. 3474.
UniPathwayiUPA00222.

Names & Taxonomyi

Protein namesi
Recommended name:
Galactosylceramide sulfotransferase (EC:2.8.2.11)
Short name:
GalCer sulfotransferase
Alternative name(s):
3'-phosphoadenosine-5'-phosphosulfate:GalCer sulfotransferase
3'-phosphoadenylylsulfate:galactosylceramide 3'-sulfotransferase
Cerebroside sulfotransferase
Gene namesi
Name:Gal3st1
Synonyms:Cst, Gcst
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1858277. Gal3st1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 12CytoplasmicSequence analysisAdd BLAST12
Transmembranei13 – 35Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST23
Topological domaini36 – 423LumenalSequence analysisAdd BLAST388

GO - Cellular componenti

  • Golgi membrane Source: MGI
  • integral component of membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000852021 – 423Galactosylceramide sulfotransferaseAdd BLAST423

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi66N-linked (GlcNAc...)Sequence analysis1
Glycosylationi312N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9JHE4.
PRIDEiQ9JHE4.

PTM databases

iPTMnetiQ9JHE4.
PhosphoSitePlusiQ9JHE4.

Expressioni

Tissue specificityi

Expressed in brain, testis, kidney, stomach, small intestine, liver, and lung. Not detected in heart, skeletal muscle, and spleen.1 Publication

Gene expression databases

BgeeiENSMUSG00000049721.
CleanExiMM_GAL3ST1.
GenevisibleiQ9JHE4. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000058348.

Structurei

3D structure databases

ProteinModelPortaliQ9JHE4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IG5S. Eukaryota.
ENOG4110RXY. LUCA.
GeneTreeiENSGT00390000013505.
HOGENOMiHOG000232148.
HOVERGENiHBG051681.
InParanoidiQ9JHE4.
KOiK01019.
OMAiDRYYDPN.
OrthoDBiEOG091G0N28.
PhylomeDBiQ9JHE4.
TreeFamiTF314802.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR009729. Gal-3-0_sulfotransfrase.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR14647. PTHR14647. 1 hit.
PfamiPF06990. Gal-3-0_sulfotr. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9JHE4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLLPKKPCK SKAKGLLLGA LFTSFLLLLY SYVVPPLYPN MAFTTSEAAA
60 70 80 90 100
PCSPIPNEPV AATPANGSAG GCQPRRDIVF MKTHKTASST LLNILFRFGQ
110 120 130 140 150
KHELKFAFPN GRNDFHYPSY FARSLVQDYR PGACFNIICN HMRFHYEEVR
160 170 180 190 200
GLVRPGATFI TVIRDPARLF ESSFHYFGSV VPLTWKLSSR DKLAEFLQDP
210 220 230 240 250
DRYYDPSSYN AHYLRNLLFF DLGYDSSLDP ASPRVQEHIL EVERRFHLVL
260 270 280 290 300
LQEYFDESLV LLRELLCWDL EDVLYFKLNA RRDSPVPRLS GELYRRATAW
310 320 330 340 350
NLLDVRLYRH FNASFWRKVE AFGRERMARE VAELRQANEH MRHICIDGGQ
360 370 380 390 400
AVGAEAIQDS AMQPWQPLGI KSILGYNLKK SIGPQHEQLC RRMLTPEIQY
410 420
LSDLGANLWV TKLWKFLRDF LRW
Length:423
Mass (Da):48,968
Last modified:July 26, 2002 - v2
Checksum:iFD54A1A71F4AEE46
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti18L → P in BAB25160 (PubMed:16141072).Curated1
Sequence conflicti263R → Q in BAB25160 (PubMed:16141072).Curated1
Sequence conflicti271E → K in BAB25160 (PubMed:16141072).Curated1
Sequence conflicti358Q → R in BAA93009 (PubMed:10727929).Curated1
Sequence conflicti358Q → R in BAA93008 (PubMed:10727929).Curated1
Sequence conflicti392R → G in BAB25160 (PubMed:16141072).Curated1
Sequence conflicti398I → T in BAA93009 (PubMed:10727929).Curated1
Sequence conflicti398I → T in BAA93008 (PubMed:10727929).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB032940 Genomic DNA. Translation: BAA93009.1.
AB032939 mRNA. Translation: BAA93008.1.
AK007645 mRNA. Translation: BAB25160.1.
BC026806 mRNA. Translation: AAH26806.1.
CCDSiCCDS24373.1.
RefSeqiNP_001171162.1. NM_001177691.1.
NP_001171174.1. NM_001177703.1.
NP_058618.2. NM_016922.3.
XP_006514819.2. XM_006514756.3.
XP_006514820.1. XM_006514757.3.
UniGeneiMm.103414.

Genome annotation databases

EnsembliENSMUST00000063004; ENSMUSP00000058348; ENSMUSG00000049721.
ENSMUST00000078757; ENSMUSP00000077815; ENSMUSG00000049721.
ENSMUST00000109981; ENSMUSP00000105608; ENSMUSG00000049721.
GeneIDi53897.
KEGGimmu:53897.
UCSCiuc007hub.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB032940 Genomic DNA. Translation: BAA93009.1.
AB032939 mRNA. Translation: BAA93008.1.
AK007645 mRNA. Translation: BAB25160.1.
BC026806 mRNA. Translation: AAH26806.1.
CCDSiCCDS24373.1.
RefSeqiNP_001171162.1. NM_001177691.1.
NP_001171174.1. NM_001177703.1.
NP_058618.2. NM_016922.3.
XP_006514819.2. XM_006514756.3.
XP_006514820.1. XM_006514757.3.
UniGeneiMm.103414.

3D structure databases

ProteinModelPortaliQ9JHE4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000058348.

PTM databases

iPTMnetiQ9JHE4.
PhosphoSitePlusiQ9JHE4.

Proteomic databases

PaxDbiQ9JHE4.
PRIDEiQ9JHE4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000063004; ENSMUSP00000058348; ENSMUSG00000049721.
ENSMUST00000078757; ENSMUSP00000077815; ENSMUSG00000049721.
ENSMUST00000109981; ENSMUSP00000105608; ENSMUSG00000049721.
GeneIDi53897.
KEGGimmu:53897.
UCSCiuc007hub.2. mouse.

Organism-specific databases

CTDi9514.
MGIiMGI:1858277. Gal3st1.

Phylogenomic databases

eggNOGiENOG410IG5S. Eukaryota.
ENOG4110RXY. LUCA.
GeneTreeiENSGT00390000013505.
HOGENOMiHOG000232148.
HOVERGENiHBG051681.
InParanoidiQ9JHE4.
KOiK01019.
OMAiDRYYDPN.
OrthoDBiEOG091G0N28.
PhylomeDBiQ9JHE4.
TreeFamiTF314802.

Enzyme and pathway databases

UniPathwayiUPA00222.
BRENDAi2.8.2.11. 3474.

Miscellaneous databases

PROiQ9JHE4.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000049721.
CleanExiMM_GAL3ST1.
GenevisibleiQ9JHE4. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR009729. Gal-3-0_sulfotransfrase.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR14647. PTHR14647. 1 hit.
PfamiPF06990. Gal-3-0_sulfotr. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiG3ST1_MOUSE
AccessioniPrimary (citable) accession number: Q9JHE4
Secondary accession number(s): Q9D8V6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: July 26, 2002
Last modified: November 30, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Mice homozygous for a null mutation of the CST gene display hindlimb weakness from week 6 of age and subsequently show a prenounced tremor and progressive ataxia. Myelin vacuolation is observed in the cerebellar white matter, diencephalon, brainstem and spinal anterior column. Male mice were infertile due to a blocked spermatogenesis.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.