Q9JHC9 (ELF2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ETS-related transcription factor Elf-2

Alternative name(s):
E74-like factor 2
New ETS-related factor

Gene names

Name:	Elf2
Synonyms:	Nerf

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	593 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probably transcriptionally activates the LYN and BLK promoters and acts synergistically with RUNX1 to transactivate the BLK promoter By similarity.
Subunit structure	Interacts with LIM domains of LMO2. Interacts via its N-terminal region with RUNX1. Ref.1 UniProtKB Q15723
Subcellular location	Nucleus.
Tissue specificity	Expressed in all tissues examined. Highest levels in thymocytes and bone marrow. Ref.1
Sequence similarities	Belongs to the ETS family. Contains 1 ETS DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: Compara positive regulation of transcription, DNA-dependent Inferred from sequence or structural similarity. Source: UniProtKB regulation of transcription from RNA polymerase II promoter Inferred from sequence or structural similarity. Source: UniProtKB transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: Compara nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 4 Ref.2 (identifier: Q9JHC9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1 Ref.1 (identifier: Q9JHC9-2)

Also known as: elf-2a;

The sequence of this isoform differs from the canonical sequence as follows:
     118-129: Missing.

Isoform 2 Ref.1 (identifier: Q9JHC9-3)

Also known as: elf-2b;

The sequence of this isoform differs from the canonical sequence as follows:
     1-79: MASAVVDSGG...QEVETENSET → MATSLHEGPTNQLDLLIRA
     118-129: Missing.

Isoform 3 Ref.2 (identifier: Q9JHC9-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-79: MASAVVDSGG...QEVETENSET → MATSLHEGPTNQLDLLIRA

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 593	593	ETS-related transcription factor Elf-2		PRO_0000204088
Regions
	DNA binding	208 – 290	83	ETS
Amino acid modifications
	Modified residue	185	1	Phosphoserine By similarity
	Modified residue	191	1	Phosphoserine By similarity
	Modified residue	372	1	Phosphoserine By similarity
	Modified residue	432	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	1 – 79	79	MASAV…ENSET → MATSLHEGPTNQLDLLIRA in isoform 2 and isoform 3. Ref.1 Ref.2		VSP_014159
	Alternative sequence	118 – 129	12	Missing in isoform 1 and isoform 2. Ref.1		VSP_014160
Experimental info
	Sequence conflict	159	1	S → W in BAC40346. Ref.3
	Sequence conflict	171	1	M → K in AAH69901. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 4 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: A6E67486141AB903	FASTA	593	63,203
	10 20 30 40 50 60 MASAVVDSGG SALELPSDGG ENQEGGDTGP DCPAVIVEPV PSARLEQGYA AQVLVYDDET 70 80 90 100 110 120 YMMQDVAEEQ EVETENSETV EASVHSSNAH CTDKTIEAAE ALLHMESPTC LRDSRSPVEV 130 140 150 160 170 180 FVPPCISTPE FIHAAMRPDV ITETVVEVST EESEPMDASP IPTSPDSHEP MKKKKVGRKP 190 200 210 220 230 240 KTQQSPVSNG SPELGIKKKA REGKGNTTYL WEFLLDLLQD KNTCPRYIKW TQREKGIFKL 250 260 270 280 290 300 VDSKAVSKLW GKHKNKPDMN YETMGRALRY YYQRGILAKV EGQRLVYQFK DMPKNIVVID 310 320 330 340 350 360 DDKSETCPED LAAAADDKSL ERVSLSAESL LKAATAVRGG KNSSPLNCSR AEKGVARVVN 370 380 390 400 410 420 ITSPTHDGSS RSPTTTAPVS AAAAPRTVRV AMQVPVVMTS LGQKISAVAV QSVNAGTGSP 430 440 450 460 470 480 LITSTSPASA SSPKVVIQTV PTVMPASTEN GDRITMQPAK IITIPATQLA QCQLQAKSNL 490 500 510 520 530 540 TGSGSINIVG TPLAVRALTP VSIAHGTPVM RLSVPAQQAS GQTPPRVISA LLKGPEGKSE 550 560 570 580 590 AKKQEHDVKT LQLVEEKGAD GNKTVTHVVV VSAPSAIALP VTMKTEGLVT CEK
	Isoform 1 (elf-2a) [UniParc]. Checksum: A39A8AB0170504ED	FASTA	581	61,933
	10 20 30 40 50 60 MASAVVDSGG SALELPSDGG ENQEGGDTGP DCPAVIVEPV PSARLEQGYA AQVLVYDDET 70 80 90 100 110 120 YMMQDVAEEQ EVETENSETV EASVHSSNAH CTDKTIEAAE ALLHMESPTC LRDSRSPEFI 130 140 150 160 170 180 HAAMRPDVIT ETVVEVSTEE SEPMDASPIP TSPDSHEPMK KKKVGRKPKT QQSPVSNGSP 190 200 210 220 230 240 ELGIKKKARE GKGNTTYLWE FLLDLLQDKN TCPRYIKWTQ REKGIFKLVD SKAVSKLWGK 250 260 270 280 290 300 HKNKPDMNYE TMGRALRYYY QRGILAKVEG QRLVYQFKDM PKNIVVIDDD KSETCPEDLA 310 320 330 340 350 360 AAADDKSLER VSLSAESLLK AATAVRGGKN SSPLNCSRAE KGVARVVNIT SPTHDGSSRS 370 380 390 400 410 420 PTTTAPVSAA AAPRTVRVAM QVPVVMTSLG QKISAVAVQS VNAGTGSPLI TSTSPASASS 430 440 450 460 470 480 PKVVIQTVPT VMPASTENGD RITMQPAKII TIPATQLAQC QLQAKSNLTG SGSINIVGTP 490 500 510 520 530 540 LAVRALTPVS IAHGTPVMRL SVPAQQASGQ TPPRVISALL KGPEGKSEAK KQEHDVKTLQ 550 560 570 580 LVEEKGADGN KTVTHVVVVS APSAIALPVT MKTEGLVTCE K
	Isoform 2 (elf-2b) [UniParc]. Checksum: 6CA5B402B609654F	FASTA	521	55,734
	10 20 30 40 50 60 MATSLHEGPT NQLDLLIRAV EASVHSSNAH CTDKTIEAAE ALLHMESPTC LRDSRSPEFI 70 80 90 100 110 120 HAAMRPDVIT ETVVEVSTEE SEPMDASPIP TSPDSHEPMK KKKVGRKPKT QQSPVSNGSP 130 140 150 160 170 180 ELGIKKKARE GKGNTTYLWE FLLDLLQDKN TCPRYIKWTQ REKGIFKLVD SKAVSKLWGK 190 200 210 220 230 240 HKNKPDMNYE TMGRALRYYY QRGILAKVEG QRLVYQFKDM PKNIVVIDDD KSETCPEDLA 250 260 270 280 290 300 AAADDKSLER VSLSAESLLK AATAVRGGKN SSPLNCSRAE KGVARVVNIT SPTHDGSSRS 310 320 330 340 350 360 PTTTAPVSAA AAPRTVRVAM QVPVVMTSLG QKISAVAVQS VNAGTGSPLI TSTSPASASS 370 380 390 400 410 420 PKVVIQTVPT VMPASTENGD RITMQPAKII TIPATQLAQC QLQAKSNLTG SGSINIVGTP 430 440 450 460 470 480 LAVRALTPVS IAHGTPVMRL SVPAQQASGQ TPPRVISALL KGPEGKSEAK KQEHDVKTLQ 490 500 510 520 LVEEKGADGN KTVTHVVVVS APSAIALPVT MKTEGLVTCE K
	Isoform 3 [UniParc]. Checksum: 778C30AB03AE0CD5	FASTA	533	57,003
	10 20 30 40 50 60 MATSLHEGPT NQLDLLIRAV EASVHSSNAH CTDKTIEAAE ALLHMESPTC LRDSRSPVEV 70 80 90 100 110 120 FVPPCISTPE FIHAAMRPDV ITETVVEVST EESEPMDASP IPTSPDSHEP MKKKKVGRKP 130 140 150 160 170 180 KTQQSPVSNG SPELGIKKKA REGKGNTTYL WEFLLDLLQD KNTCPRYIKW TQREKGIFKL 190 200 210 220 230 240 VDSKAVSKLW GKHKNKPDMN YETMGRALRY YYQRGILAKV EGQRLVYQFK DMPKNIVVID 250 260 270 280 290 300 DDKSETCPED LAAAADDKSL ERVSLSAESL LKAATAVRGG KNSSPLNCSR AEKGVARVVN 310 320 330 340 350 360 ITSPTHDGSS RSPTTTAPVS AAAAPRTVRV AMQVPVVMTS LGQKISAVAV QSVNAGTGSP 370 380 390 400 410 420 LITSTSPASA SSPKVVIQTV PTVMPASTEN GDRITMQPAK IITIPATQLA QCQLQAKSNL 430 440 450 460 470 480 TGSGSINIVG TPLAVRALTP VSIAHGTPVM RLSVPAQQAS GQTPPRVISA LLKGPEGKSE 490 500 510 520 530 AKKQEHDVKT LQLVEEKGAD GNKTVTHVVV VSAPSAIALP VTMKTEGLVT CEK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Elf-2, a rhombotin-2 binding ets transcription factor: discovery and potential role in T cell leukemia." Wilkinson D.A., Neale G.A.M., Mao S., Naeve C.W., Goorha R.M. Leukemia 11:86-96(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY, INTERACTION WITH LMO2. Tissue: T-cell.
[2]	Wilkinson D.A., Neale G.A.M., Mao S., Fernandes E.R., Davenport J.W., Naeve C.W., Goorha R.M. Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 3 AND 4).
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: NOD. Tissue: Thymus.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-175 (ISOFORM 1). Strain: NMRI. Tissue: Mammary gland.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF256216 mRNA. Translation: AAF67191.1. AF256217 mRNA. Translation: AAF67192.1. AF256218 mRNA. Translation: AAF67193.1. AF256219 mRNA. Translation: AAF67194.1. AK088422 mRNA. Translation: BAC40346.1. BC069901 mRNA. Translation: AAH69901.1.
IPI	IPI00119253. IPI00607912. IPI00607942. IPI00608103.
RefSeq	NP_075991.1. NM_023502.1.
UniGene	Mm.131038.
3D structure databases
ProteinModelPortal	Q9JHC9.
SMR	Q9JHC9. Positions 210-289.
ModBase	Search...
PTM databases
PhosphoSite	Q9JHC9.
Proteomic databases
PaxDb	Q9JHC9.
PRIDE	Q9JHC9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000062009; ENSMUSP00000061076; ENSMUSG00000037174. ENSMUST00000091144; ENSMUSP00000088678; ENSMUSG00000037174. ENSMUST00000108051; ENSMUSP00000103686; ENSMUSG00000037174. ENSMUST00000108053; ENSMUSP00000103688; ENSMUSG00000037174. ENSMUST00000163748; ENSMUSP00000126871; ENSMUSG00000037174.
GeneID	69257.
KEGG	mmu:69257.
UCSC	uc008pdo.1. mouse. uc008pdp.1. mouse. uc008pdr.1. mouse.
Organism-specific databases
CTD	1998.
MGI	MGI:1916507. Elf2.
Phylogenomic databases
eggNOG	NOG291231.
GeneTree	ENSGT00700000104115.
HOGENOM	HOG000049253.
HOVERGEN	HBG007183.
InParanoid	Q9JHC9.
KO	K09428.
OMA	CNEDLAA.
OrthoDB	EOG4HX50W.
Gene expression databases
ArrayExpress	Q9JHC9.
Bgee	Q9JHC9.
CleanEx	MM_ELF2.
Genevestigator	Q9JHC9.
GermOnline	ENSMUSG00000037174. Mus musculus.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
InterPro	IPR000418. Ets_dom. IPR022084. TF_Elf_N. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF12310. Elf-1_N. 1 hit. PF00178. Ets. 1 hit. [Graphical view]
PRINTS	PR00454. ETSDOMAIN.
SMART	SM00413. ETS. 1 hit. [Graphical view]
PROSITE	PS00345. ETS_DOMAIN_1. 1 hit. PS00346. ETS_DOMAIN_2. 1 hit. PS50061. ETS_DOMAIN_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	328977.
SOURCE	Search...

Entry information

Entry name

ELF2_MOUSE

Accession

Primary (citable) accession number: Q9JHC9
Secondary accession number(s): Q6NST2 , Q8BTX8, Q9JHC7, Q9JHC8, Q9JHD0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program