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Protein

Metalloproteinase inhibitor 4

Gene

Timp4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi30 – 301Zinc; via amino nitrogen and carbonyl oxygen; shared with metalloproteinase partnerBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Metalloenzyme inhibitor, Metalloprotease inhibitor, Protease inhibitor

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiI35.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Metalloproteinase inhibitor 4
Alternative name(s):
Tissue inhibitor of metalloproteinases 4
Short name:
TIMP-4
Gene namesi
Name:Timp4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:109125. Timp4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Sequence analysisAdd
BLAST
Chaini30 – 224195Metalloproteinase inhibitor 4PRO_0000034350Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi30 ↔ 102PROSITE-ProRule annotation
Disulfide bondi32 ↔ 131PROSITE-ProRule annotation
Disulfide bondi42 ↔ 156PROSITE-ProRule annotation
Disulfide bondi158 ↔ 205PROSITE-ProRule annotation
Disulfide bondi163 ↔ 168PROSITE-ProRule annotation
Disulfide bondi176 ↔ 197PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiQ9JHB3.
PaxDbiQ9JHB3.
PRIDEiQ9JHB3.

Expressioni

Tissue specificityi

Expressed in brain, heart, ovary and skeletal muscle.

Gene expression databases

BgeeiQ9JHB3.
CleanExiMM_TIMP4.
ExpressionAtlasiQ9JHB3. baseline and differential.
GenevisibleiQ9JHB3. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000032462.

Structurei

3D structure databases

ProteinModelPortaliQ9JHB3.
SMRiQ9JHB3. Positions 30-210.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini30 – 156127NTRPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni30 – 345Involved in metalloproteinase-bindingBy similarity
Regioni99 – 1002Involved in metalloproteinase-bindingBy similarity

Sequence similaritiesi

Contains 1 NTR domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4745. Eukaryota.
ENOG41103NU. LUCA.
GeneTreeiENSGT00390000004555.
HOGENOMiHOG000285981.
HOVERGENiHBG068749.
InParanoidiQ9JHB3.
OMAiTISAPNE.
OrthoDBiEOG79GT74.
PhylomeDBiQ9JHB3.
TreeFamiTF317409.

Family and domain databases

Gene3Di3.90.370.10. 1 hit.
InterProiIPR001134. Netrin_domain.
IPR001820. TIMP.
IPR008993. TIMP-like_OB-fold.
IPR027465. TIMP_C.
IPR030490. TIMP_CS.
[Graphical view]
PANTHERiPTHR11844. PTHR11844. 1 hit.
PfamiPF00965. TIMP. 1 hit.
[Graphical view]
SMARTiSM00206. NTR. 1 hit.
[Graphical view]
SUPFAMiSSF50242. SSF50242. 1 hit.
PROSITEiPS50189. NTR. 1 hit.
PS00288. TIMP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9JHB3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPWSPLAALS WALVLRLLAL LWPPGRGEAC SCAPAHPQQH FCHSALVIRA
60 70 80 90 100
KISSEKVVPA SKDPADTQKL IRYEIKQIKM FKGFEKAKDI QYVYTPFDSS
110 120 130 140 150
LCGVKLETNS HKQYLLTGQI LSDGKVFIHL CNYIEPWEDL SLVQRESLNH
160 170 180 190 200
HYHQNCGCQI TTCYAVPCTI SAPNECLWTD WLLERKLYGY QAQHYVCMKH
210 220
VDGICSWYRG HLHLRKEYVD IIQP
Length:224
Mass (Da):25,774
Last modified:October 1, 2000 - v1
Checksum:iD66867C71E0C4AD9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti118 – 1236GQILSD → ARFSG in AAK62886 (Ref. 2) Curated
Sequence conflicti130 – 1389LCNYIEPWE → FVPTYIKPWK in AAK62886 (Ref. 2) Curated
Sequence conflicti156 – 1561C → L in AAK62886 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF282730 mRNA. Translation: AAF97239.1.
AF345865, AF345864 Genomic DNA. Translation: AAK62886.1.
BC064046 mRNA. Translation: AAH64046.1.
CCDSiCCDS20438.1.
RefSeqiNP_542370.3. NM_080639.3.
UniGeneiMm.255607.

Genome annotation databases

EnsembliENSMUST00000032462; ENSMUSP00000032462; ENSMUSG00000030317.
GeneIDi110595.
KEGGimmu:110595.
UCSCiuc009dip.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF282730 mRNA. Translation: AAF97239.1.
AF345865, AF345864 Genomic DNA. Translation: AAK62886.1.
BC064046 mRNA. Translation: AAH64046.1.
CCDSiCCDS20438.1.
RefSeqiNP_542370.3. NM_080639.3.
UniGeneiMm.255607.

3D structure databases

ProteinModelPortaliQ9JHB3.
SMRiQ9JHB3. Positions 30-210.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000032462.

Protein family/group databases

MEROPSiI35.004.

Proteomic databases

MaxQBiQ9JHB3.
PaxDbiQ9JHB3.
PRIDEiQ9JHB3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032462; ENSMUSP00000032462; ENSMUSG00000030317.
GeneIDi110595.
KEGGimmu:110595.
UCSCiuc009dip.1. mouse.

Organism-specific databases

CTDi7079.
MGIiMGI:109125. Timp4.

Phylogenomic databases

eggNOGiKOG4745. Eukaryota.
ENOG41103NU. LUCA.
GeneTreeiENSGT00390000004555.
HOGENOMiHOG000285981.
HOVERGENiHBG068749.
InParanoidiQ9JHB3.
OMAiTISAPNE.
OrthoDBiEOG79GT74.
PhylomeDBiQ9JHB3.
TreeFamiTF317409.

Miscellaneous databases

NextBioi364299.
PROiQ9JHB3.
SOURCEiSearch...

Gene expression databases

BgeeiQ9JHB3.
CleanExiMM_TIMP4.
ExpressionAtlasiQ9JHB3. baseline and differential.
GenevisibleiQ9JHB3. MM.

Family and domain databases

Gene3Di3.90.370.10. 1 hit.
InterProiIPR001134. Netrin_domain.
IPR001820. TIMP.
IPR008993. TIMP-like_OB-fold.
IPR027465. TIMP_C.
IPR030490. TIMP_CS.
[Graphical view]
PANTHERiPTHR11844. PTHR11844. 1 hit.
PfamiPF00965. TIMP. 1 hit.
[Graphical view]
SMARTiSM00206. NTR. 1 hit.
[Graphical view]
SUPFAMiSSF50242. SSF50242. 1 hit.
PROSITEiPS50189. NTR. 1 hit.
PS00288. TIMP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Murine tissue inhibitor of metalloproteinases-4 (Timp-4): cDNA isolation and expression in adult mouse tissues."
    Leco K.J., Apte S.S., Taniguchi G.T., Hawkes S.P., Khokha R., Schultz G.A., Edwards D.R.
    FEBS Lett. 401:213-217(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: CD-1.
    Tissue: Brain and Heart.
  2. Rahkonen O.P., Koskivirta I.M., Vuorio E.I.
    Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/SvJ.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Mammary gland.

Entry informationi

Entry nameiTIMP4_MOUSE
AccessioniPrimary (citable) accession number: Q9JHB3
Secondary accession number(s): Q924B7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2002
Last sequence update: October 1, 2000
Last modified: May 11, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.