Q9JH69 (NS1AB_TASV1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-structural polyprotein 1AB

Cleaved into the following 5 chains:

1. Protein p19
2. Transmembrane protein 1A
3. Serine protease p27
   Short name=p27
   EC=3.4.21.-
4. Protein p20
5. RNA-directed RNA polymerase p57
   Short name=p57
   EC=2.7.7.48

Gene names

Name:

ORF1

Organism

Turkey astrovirus 1 (TAstV-1)

Taxonomic identifier

364370 [NCBI]

Taxonomic lineage

Viruses › ssRNA positive-strand viruses, no DNA stage › Astroviridae › Avastrovirus

Virus host

Meleagris gallopavo (Common turkey) [TaxID: 9103]

Protein attributes

Sequence length	1611 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Non-structural polyprotein 1AB encodes for protease and genome replication/transcription proteins By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subcellular location	Host membrane; Multi-pass membrane protein Potential.
Post-translational modification	Cleaved presumably by viral and host proteases By similarity.
Sequence similarities	Belongs to the astroviridae polyprotein 1AB family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication
Cellular component	Host membrane Membrane
Coding sequence diversity	Ribosomal frameshifting
Domain	Coiled coil Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Nucleotidyltransferase Protease RNA-directed RNA polymerase Thiol protease Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro viral genome replication Inferred from electronic annotation. Source: InterPro
Cellular component	host cell membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by ribosomal frameshifting. [Align] [Select]

Isoform nsp1ab (identifier: Q9JH69-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Generated by a ribosomal frameshift at position 1099.

Isoform nsp1a (identifier: Q9JH70-1)

The sequence of this isoform can be found in the external entry Q9JH70.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1611	1611	Non-structural polyprotein 1AB		PRO_0000327307
	Chain	1 – 168	168	Protein p19 Potential		PRO_0000327308
	Chain	169 – 508	340	Transmembrane protein 1A Potential		PRO_0000327309
	Chain	509 – 795	287	Serine protease p27 Potential		PRO_0000327310
	Chain	796 – 1027	232	Protein p20 Potential		PRO_0000327311
	Chain	1028 – 1611	584	RNA-directed RNA polymerase p57 Potential		PRO_0000327312
Regions
	Transmembrane	195 – 215	21	Helical; Potential
	Transmembrane	329 – 348	20	Helical; Potential
	Transmembrane	353 – 373	21	Helical; Potential
	Transmembrane	375 – 395	21	Helical; Potential
	Transmembrane	397 – 417	21	Helical; Potential
	Transmembrane	426 – 446	21	Helical; Potential
	Transmembrane	450 – 470	21	Helical; Potential
	Domain	1352 – 1486	135	RdRp catalytic
	Coiled coil	124 – 187	64	Potential
Sites
	Active site	550	1	Charge relay system; for serine protease activity By similarity
	Active site	582	1	Charge relay system; for serine protease activity By similarity
	Active site	647	1	Charge relay system; for serine protease activity By similarity
	Site	168 – 169	2	Cleavage Potential
	Site	508 – 509	2	Cleavage Potential
	Site	795 – 796	2	Cleavage Potential
	Site	1027 – 1028	2	Cleavage Potential

Sequences

	Sequence		Length	Mass (Da)
	Isoform nsp1ab [UniParc]. Last modified April 8, 2008. Version 3. Checksum: F3827B808E34A58F	FASTA	1,611	183,203
	10 20 30 40 50 60 MAAAAASALG ASAPKALAPA DGPIVAGLDK LVNLEGVHDL FEAMRGAYGE DPAWKGLMSC 70 80 90 100 110 120 DVVYLKDITT AIGVKDTSVG IFRKFSDGCS WCPTGAECFL SMKDLAYMKA QSAKAQRLTA 130 140 150 160 170 180 SLATTSNLIA RAMRAESELK RARDEERKVD ARYKDILEHS LAARKALQKE LDETRERELH 190 200 210 220 230 240 LLKELGKRSS IRTKAFSFFD WLFMAVVFFL FLHYTSAECV KPDFGCLVVN SNLPVPSLTF 250 260 270 280 290 300 HDVMARCYNT FGNIVLSSQI DAARLREECE QSANKFLGTH IGDPAHKVWC ENRLETLIPV 310 320 330 340 350 360 ECDSSEFLEI FTSNLNAFMV SVSQFYKTIS YYKLDALVTF AFSAALATNK LKMVMVLPLL 370 380 390 400 410 420 LVALYLNVPP ITVTIASVIF QPLILPFVGF QLVFPNFLPY NLFVAWVWMV CQAFFSSDGV 430 440 450 460 470 480 KLLVSVSTAL VQVVFLAVWS ISVIVLQQLS IPMVAQILLF VATLTVSVGV KFANSTITVV 490 500 510 520 530 540 HPDGNTEKVS RVTLVRQSMA KRISQIKQSL TIRGVIPSGP NRFDSIVVVE GQGGSGVGWR 550 560 570 580 590 600 FMNSIFTAGH VVQGSKFVTI KSESTQVKVK VKRVIDLFEC VDTLVEIPLT KEFQHIKPLR 610 620 630 640 650 660 LAKKVEDSYL QLCAFKPDMV EKASYQGWCT IDSGFIFNSF NTQFGNSGAP YVDSDGRLVG 670 680 690 700 710 720 MHLGSQGVIS QGVVLVDTLK TQFLAQQSQI DDQLMERIIE GTKVSHAAIL TELDRMRTKV 730 740 750 760 770 780 EEVALVSARV NQLESQLKDL YEFSSNSIKC LSDDIEKMVC AQLFDEINLQ SVMEKISALP 790 800 810 820 830 840 PTEKLAKLVE VFVEQKKKGK TKRTARGGKH ALGKKYLSKA HFSRMRMLTE EEYNKMVEDG 850 860 870 880 890 900 FSPDEIKEVV DQLREQAWQN YLIDNDIGED DDLDWYDDML EDERLNEEID RRVEAALEDR 910 920 930 940 950 960 GELAYQKIRR TFVDQALIHL ITLKKGNWQT TKVECQPERE EAYKEQFQKA VKQEDLTEGT 970 980 990 1000 1010 1020 SYAIYSAGDA TILIENKEID HTEIKPVTTG AKTVQEYPKD ARTTVATFDD NKKDIVKTKR 1030 1040 1050 1060 1070 1080 TTEIVLEQRK KTCRTCGETR PHNHKMCRDR HTRRFCFWCG VVHSDVEGHS RDLKCPKCSA 1090 1100 1110 1120 1130 1140 GFANLREMEQ HAVTTCSKKL DSHPEPSRVF QPLDFGLGIF DWRFDLQPIR HHVAVPMNVE 1150 1160 1170 1180 1190 1200 VLGYIPVDRL VERRNVITDP LLKLVEPWRQ ETYGPAVWTI KAYNKMFEKF FYSEPLEFAQ 1210 1220 1230 1240 1250 1260 LDSSILNLAD SYCLQEHDYM SGSQIVPITS TEKNLDSTPG YPKFKVFSTE REYLSTCGWD 1270 1280 1290 1300 1310 1320 EYKTVWQVGP REKPLWWCFL KTEVLKLAKI EQDDIRMILC TDPVFTRIGA AFEQHQNSLM 1330 1340 1350 1360 1370 1380 KLETENHHAQ VGWSPFFGGI HRRATRLYGE HRYYVELDWT RFDGTIPPEL FRRIKLMRFF 1390 1400 1410 1420 1430 1440 LLDPKYKTPE NRDRYNWYVE NLIDKVVLLP TGEVCKIYGG NPSGQFSTTV DNNFVNVWLT 1450 1460 1470 1480 1490 1500 VFELAYLFYK EHNRLPTICE IKKHTDWICY GDDRLLAVDK RFINSYDTAA VIAMYKDVFG 1510 1520 1530 1540 1550 1560 MWVKPDNIKV FPSLEGVSFC GMVWTKRKGQ YVGKPNVDKI LSTLSDPVSR LPDIQSLWGK 1570 1580 1590 1600 1610 LVSLRLLCEN ESDEVVDYLD KQIESVSRHA KEAGIALPKI GPDFYAEIWI D
	Isoform nsp1a [UniParc]. See Q9JH70.		–	–

References

[1]	"Complete genomic sequences of astroviruses from sheep and turkey: comparison with related viruses." Jonassen C.M., Jonassen T.O., Sveen T.M., Grinde B. Virus Res. 91:195-201(2003) [PubMed: 12573498] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y15936 Genomic RNA. Translation: CAB95006.3. Sequence problems.
3D structure databases
ProteinModelPortal	Q9JH69.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR009003. Pept_cys/ser_Trypsin-like. IPR001205. RNA-dir_pol_picornavirus. IPR007094. RNA-dir_pol_PSvirus. [Graphical view]
Pfam	PF00680. RdRP_1. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50507. RDRP_SSRNA_POS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NS1AB_TASV1

Accession

Primary (citable) accession number: Q9JH69

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	April 8, 2008
Last modified:	November 16, 2011
This is version 53 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program