Reviewed, UniProtKB/Swiss-Prot Q9JH66 (NS1AB_OASV1)

Names and origin

Protein names

Recommended name:
    Non-structural polyprotein 1AB
Cleaved into the following 5 chains:
    1- Recommended name:
            Protein p19
    2- Recommended name:
            Transmembrane protein 1A
    3- Recommended name:
            Serine protease p27
                Short name=p27
              EC=3.4.21.-
    4- Recommended name:
            Protein p20
    5- Recommended name:
            RNA-directed RNA polymerase p57
                Short name=p57
              EC=2.7.7.48

Gene names

Name:

ORF1

Organism

Ovine astrovirus 1 (OAstV-1)

Taxonomic identifier

70794 [NCBI]

Taxonomic lineage

Viruses › ssRNA positive-strand viruses, no DNA stage › Astroviridae › Mamastrovirus

Virus host

Ovis aries (Sheep) [TaxID: 9940]

Protein attributes

Sequence length	1351 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Non-structural polyprotein 1AB contains the viral protease participating in the cleavage of the polyprotein into functional products. It contains also the activities necessary for replication of genomic RNA, as well as transcription of subgenomic mRNA By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subcellular location	Host membrane; Multi-pass membrane protein Potential.
Post-translational modification	Cleaved presumably by viral and host proteases By similarity.
Sequence similarities	Belongs to the astroviridae polyprotein 1AB family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication
Cellular component	Membrane
Coding sequence diversity	Ribosomal frameshifting
Domain	Coiled coil Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Nucleotidyltransferase Protease RNA-directed RNA polymerase Thiol protease Transferase
Gene Ontology (GO)
Biological process	transcription, RNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW viral genome replication Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by ribosomal frameshifting. [Align] [Select]

Isoform nsp1ab (identifier: Q9JH66-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Generated by a ribosomal frameshift at position 830.

Isoform nsp1a (identifier: Q9JH67-1)

The sequence of this isoform can be found in the external entry Q9JH67-1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1351	1351	Non-structural polyprotein 1AB		PRO_0000327301
	Chain	1 – 172	172	Protein p19 Potential		PRO_0000327302
	Chain	173 – 407	235	Transmembrane protein 1A Potential		PRO_0000327303
	Chain	408 – 640	233	Serine protease p27 Potential		PRO_0000327304
	Chain	641 – 826	186	Protein p20 Potential		PRO_0000327305
	Chain	827 – 1351	525	RNA-directed RNA polymerase p57 Potential		PRO_0000327306
Regions
	Transmembrane	153 – 173	21	Potential
	Transmembrane	231 – 251	21	Potential
	Transmembrane	257 – 277	21	Potential
	Transmembrane	304 – 324	21	Potential
	Transmembrane	337 – 357	21	Potential
	Domain	1085 – 1217	133	RdRp catalytic
	Coiled coil	105 – 144	40	Potential
	Coiled coil	579 – 635	57	Potential
Sites
	Active site	452	1	Charge relay system; for serine protease activity By similarity
	Active site	481	1	Charge relay system; for serine protease activity By similarity
	Active site	544	1	Charge relay system; for serine protease activity By similarity
	Site	172 – 173	2	Cleavage Potential
	Site	407 – 408	2	Cleavage Potential
	Site	640 – 641	2	Cleavage Potential
	Site	826 – 827	2	Cleavage Potential

Sequences

	Sequence		Length	Mass (Da)
	Isoform nsp1ab [UniParc]. Last modified April 8, 2008. Version 2. Checksum: FB0A954B26292C54	FASTA	1,351	154,740
	10 20 30 40 50 60 MNVYDKVLQF GSKKARARGM ALNKLSRNRL EDIYAGSGPL VFGFGPIDMV DPGSLNPSIK 70 80 90 100 110 120 TLDTVYVAAV QPDNQYVVHH FVPGRNEWVE TDASTHQPTA LVGVLVQDHK AKTREVEDLK 130 140 150 160 170 180 SQLSQLRMEH EILRHEYERL KLKSPIVKPF KPLKVLLFSL LLGLLFAGVT NGARTGTCYA 190 200 210 220 230 240 YDEEKDTCLY WEWKDSREVA WYDSYVTEAL AIYNRACVYV RSKEFMTYLS LVFQTVFNWY 250 260 270 280 290 300 FCATALAVYY MARAENPIVM FVTLALATLS QFQLLAVAVL PLLDFSATMG LWLSMVVFYM 310 320 330 340 350 360 SQQISILVSF CVLVLSVMIG TFMADSEYAM MIKGHAVVFA IVCYSHVAMI LNIPPWVVSL 370 380 390 400 410 420 TMVCYRLWRV CFVFPAERLE IRSADGKVLH TVPTHPNWTA KVTRFVQSLR KGLRTSVAPT 430 440 450 460 470 480 ARIVPDGIAI VEAREGVGTC FRVKNNLVTS KHVVGSDDAV KIRWGAQEDM ARVTYRHPTK 490 500 510 520 530 540 DIALMALPTN LQTMPAYKFA KAITDGPIVM TAFDEANLLL VAVTEGVRVE DHMTYSVATR 550 560 570 580 590 600 NGMSGAPITT VDGRVIAVHQ TNTGFTGGAV IFVPEDIPEV RKISKREQEL EDRVKQLEGM 610 620 630 640 650 660 LNMDQAYVDS NLIVDLVREA VQREMKVLRT ELANLGGFSQ KKKGKNKSTK RKRKAVWTEE 670 680 690 700 710 720 EYKAMLEKGF TRDQLRIMAD AIRDQYYDDE DEQSEEEAGY PDWSDPGDST DIENEWFGYE 730 740 750 760 770 780 QSWKELEPAK SGVVVNTLPK DLVFKYSLDN YPISKQDIQA VAKELKIYEK AISDIISTSV 790 800 810 820 830 840 STDGKWKDDV DAQKILQELD GLWWGINHTL WEHGLMPFTQ RRKRVQQPKK LQRGPEDPGP 850 860 870 880 890 900 EECKLDYWEQ LVEPSKEKFL VPPEYPLLGV VPLDRPISDF DAPVDNLLAL LPEPESPDLG 910 920 930 940 950 960 FEPAVWGPEA YVKSFEKFDF ADPDPNIEKN YPREWAFANL VLHREFDFLA DSVVKDITAT 970 980 990 1000 1010 1020 SKNSESTPGF PKTYWWKTEA EYLAKRGYAD YVSEWNRIRG GARPNVLWYL FLKKEILKST 1030 1040 1050 1060 1070 1080 KVRDADIRQI ICSDPIFARI GCCFEEDQNE RMKRRTKTRM PQCGWSPFFG GFNDRIQRLV 1090 1100 1110 1120 1130 1140 AKGNPYWIEF DWTRYDGTIP SQIFKHIKNF RFSMLAKEYQ TPELRNMYHW YVDNILRRYV 1150 1160 1170 1180 1190 1200 CMPSGEITIQ HKGNPSGQVS TTMDNNLVNV FLQAFEYAYL HPEKSMDELR KDWESYDSLI 1210 1220 1230 1240 1250 1260 YGDDRLTTSP SVPNDYVTRV VAMYKDIFGM WVKPEKVKVS HSPVGLSFCG FVITHQDGQY 1270 1280 1290 1300 1310 1320 LPVPAEEAKL LASLLRPTKK LENMDALYGK LLCYRILNHN LPNDNKFRNY ILVALEVMAR 1330 1340 1350 HYSSRGEEPP FYVTESMLDK LWRGGPKFDY G
	Isoform nsp1a. See Q9JH67.	FASTA	–	–

References

[1]	"Complete genomic sequences of astroviruses from sheep and turkey: comparison with related viruses." Jonassen C.M., Jonassen T.O., Sveen T.M., Grinde B. Virus Res. 91:195-201(2003) [PubMed: 12573498] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y15937 Genomic RNA. Translation: CAB95003.1. Sequence problems.
RefSeq	NP_059945.2.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1449587.
Family and domain databases
InterPro	IPR007094. RNA_pol_PSvir. [Graphical view]
PROSITE	PS50507. RDRP_SSRNA_POS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NS1AB_OASV1

Accession

Primary (citable) accession number: Q9JH66

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	April 8, 2008
Last modified:	June 16, 2009
This is version 32 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)