Reviewed, UniProtKB/Swiss-Prot Q9JGF2 (NS1AB_ANV1)

Names and origin

Protein names

Recommended name:
    Non-structural polyprotein 1AB
Cleaved into the following 5 chains:
    1- Recommended name:
            Protein p19
    2- Recommended name:
            Transmembrane protein 1A
    3- Recommended name:
            Serine protease p27
                Short name=p27
              EC=3.4.21.-
    4- Recommended name:
            Protein p20
    5- Recommended name:
            RNA-directed RNA polymerase p57
                Short name=p57
              EC=2.7.7.48

Gene names

Name:

ORF1

Organism

Avian nephritis virus 1 (ANV-1)

Taxonomic identifier

336960 [NCBI]

Taxonomic lineage

Viruses › ssRNA positive-strand viruses, no DNA stage › Astroviridae › Avastrovirus

Virus host

Gallus gallus (Chicken) [TaxID: 9031]
Meleagris gallopavo (Common turkey) [TaxID: 9103]

Protein attributes

Sequence length	1512 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Non-structural polyprotein 1AB contains the viral protease participating in the cleavage of the polyprotein into functional products. It contains also the activities necessary for replication of genomic RNA, as well as transcription of subgenomic mRNA By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subcellular location	Host membrane; Multi-pass membrane protein Potential.
Post-translational modification	Cleaved presumably by viral and host proteases By similarity.
Sequence similarities	Belongs to the astroviridae polyprotein 1AB family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication
Cellular component	Membrane
Coding sequence diversity	Ribosomal frameshifting
Domain	Coiled coil Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Nucleotidyltransferase Protease RNA-directed RNA polymerase Thiol protease Transferase
Gene Ontology (GO)
Biological process	transcription, RNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW viral genome replication Inferred from electronic annotation. Source: InterPro viral protein processing Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by ribosomal frameshifting. [Align] [Select]

Isoform nsp1ab (identifier: Q9JGF2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Generated by a ribosomal frameshift at position 1005.

Isoform nsp1a (identifier: P0C6K7-1)

The sequence of this isoform can be found in the external entry P0C6K7-1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1512	1512	Non-structural polyprotein 1AB		PRO_0000327319
	Chain	1 – 204	204	Protein p19 Potential		PRO_0000327320
	Chain	205 – 478	274	Transmembrane protein 1A Potential		PRO_0000327321
	Chain	479 – 730	252	Serine protease p27 Potential		PRO_0000327322
	Chain	731 – 954	224	Protein p20 Potential		PRO_0000327323
	Chain	955 – 1512	558	RNA-directed RNA polymerase p57 Potential		PRO_0000327324
Regions
	Transmembrane	190 – 210	21	Potential
	Transmembrane	295 – 315	21	Potential
	Transmembrane	323 – 343	21	Potential
	Transmembrane	360 – 380	21	Potential
	Transmembrane	399 – 419	21	Potential
	Domain	1257 – 1390	134	RdRp catalytic
Sites
	Active site	524	1	Charge relay system; for serine protease activity By similarity
	Active site	556	1	Charge relay system; for serine protease activity By similarity
	Active site	621	1	Charge relay system; for serine protease activity By similarity
	Site	204 – 205	2	Cleavage Potential
	Site	478 – 479	2	Cleavage Potential
	Site	730 – 731	2	Cleavage Potential
	Site	954 – 955	2	Cleavage Potential

Sequences

	Sequence		Length	Mass (Da)
	Isoform nsp1ab [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 195D9D1DC048DCDC	FASTA	1,512	171,273
	10 20 30 40 50 60 MASAGPTGAG ARPPKAFTAQ AGLAKLVNPA GLNSILARGK EKFGGTQAWK ELMGCDVIFA 70 80 90 100 110 120 RSISHWYGIK GTTYYELTVA LGQPLYKPVT DPELTEEEKA VMTAVQSRFA QSNSSVVLTR 130 140 150 160 170 180 TLLNKTCELK DRIRELTDEL GQTEVHLARE KVKAAALKLE NRKLFVENQE LKDQLEKERT 190 200 210 220 230 240 KHGWKGLKTL CLWIFLATLI GGYITGSNAA CTLVDVPSPM KVGYDTFKQM CIHKDSYLPD 250 260 270 280 290 300 GAFDKESLAL ECSKQMDYMD CKEVITDSIS GKTSFAGMLR DVFRVDEIVT AIRTVVRFAM 310 320 330 340 350 360 DFSLAYPICV MFVLILTRNK KHAIISACCA LVAKCCGLRL LPFTLVLTYA PSETAIAGCI 370 380 390 400 410 420 YGLGYISIPL VTFLHWVGLV LKAILVPDDC YIGTRVSHAL AWSIMLPMWI ITQELMAFTE 430 440 450 460 470 480 FPLELQIVTT VVVCTAGFGF RYLTGTVTIT EPDGTVKKYK RIFNAKSAIG TISTVFFEKA 490 500 510 520 530 540 KAIRGVIPSF PSKADNIVKI EVDVDGGSAG VGFRLGNYIY TAGHVVGEAK IAKITWKGLT 550 560 570 580 590 600 SQAKVLGHIE LPLFTDTLAR LEIPKPFQQL PVFRLAKSSE NDYVQMVCFD NQLQNVVTFS 610 620 630 640 650 660 GWANIDGDYL NAPFETYAGT SGSPIINRDG RMLGVHFGSN AVVSQGFVIT RLFATEPAVK 670 680 690 700 710 720 QCKSDEDLAD EIVRKVMGGI RISFASLTSE LEKQRDELNA LKQMVNDLID TDLVALEKKK 730 740 750 760 770 780 GKTKRTVRGQ KHKTKAISKA AFMKTKVLTE EEYRRLEEEG FTKDEIKDIV DNLREQAWLD 790 800 810 820 830 840 YQNQLDEEGD DDWYEQMEED QRINDQIDQN IERDLEDRGE WYGQRKITFK QRAMLRFIQL 850 860 870 880 890 900 GRQQQVATVS FPDGYEDRAE ELYNKVVTTE DLPEGETSEA ALSLPNKIVH QAGKRLNFKH 910 920 930 940 950 960 VKIHPDKTFM KSGVTQIEEK PEGDIILKAK TTTLAPKEEP VIQQVEQQPQ VEQQQQPQQP 970 980 990 1000 1010 1020 VVEEKKRTPP PKPQRKPKTG AKAKCLDCGE TFVERQDFHV CKSKKLNEPP SGGYTPVPDH 1030 1040 1050 1060 1070 1080 LRWNNWQIYM EPLDLRITVP ENYPILGHIA IDKLVERKKK VNDPLLKMLE QPKCEGFTST 1090 1100 1110 1120 1130 1140 TWTRKAYTKS FEKFDYGDAV DFVQDYPELT AFADAAVLAE VGYMEGTHVI PIQETSKNMD 1150 1160 1170 1180 1190 1200 STPAFPKMLD FDSERDYLEA HGMKEYIDTQ LGVQSGKPLW WCFLKNEILK EKKVSEDDIR 1210 1220 1230 1240 1250 1260 IITCSDPVIT RLGASFDSEQ NERMKERTET HHAQVGWTPF FGGLDKRVRR ITSCGRTQVL 1270 1280 1290 1300 1310 1320 ELDWTRFDGT IPVQLFQRMR ELRKFFLTRR SRRRYGKLLD WYNAQLTDRI TLLPTGEVTH 1330 1340 1350 1360 1370 1380 VKKGNPSGQF STTVDNNLVN EWLTAFEFGY QHLENHGIIP TVRDYRANVD FLCYGDDRLL 1390 1400 1410 1420 1430 1440 AFNPSFVNYD PQVTIDMYKN IFGMWVKPEN IKLFDSPTGS SFCGFTLVKP HGQWVGVVNV 1450 1460 1470 1480 1490 1500 NKLLQSLKTP TRRLPDLESL WGKLVSLKIM CYHSDPEAVS YLSNQIRRVE EYARAEGIEL 1510 PEVGPDFYRK IW
	Isoform nsp1a. See P0C6K7.	FASTA	–	–

References

[1]

"Avian nephritis virus (ANV) as a new member of the family Astroviridae and construction of infectious ANV cDNA." Imada T., Yamaguchi S., Mase M., Tsukamoto K., Kubo M., Morooka A. J. Virol. 74:8487-8493(2000) [PubMed: 10954549] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: G-4260.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB033998 Genomic RNA. Translation: BAA92848.1. Sequence problems.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	S01.109.
Family and domain databases
InterPro	IPR008760. Peptidase_S32. IPR001205. RNA_pol_P3D. IPR007094. RNA_pol_PSvir. [Graphical view]
Pfam	PF05579. Peptidase_S32. 1 hit. PF00680. RdRP_1. 1 hit. [Graphical view]
PROSITE	PS50507. RDRP_SSRNA_POS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NS1AB_ANV1

Accession

Primary (citable) accession number: Q9JGF2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)