Q9JFM9 (FLSO_ASFM2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
FAD-linked sulfhydryl oxidase
EC=1.8.3.2

Alternative name(s):
p14

Gene names

Ordered Locus Names:	Mal-081
ORF Names:	L09GL

Organism

African swine fever virus (isolate Tick/Malawi/Lil 20-1/1983) (ASFV) [Complete proteome]

Taxonomic identifier

10500 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Asfarviridae › Asfivirus

Virus host

Ornithodoros (relapsing fever ticks) [TaxID: 6937]
Sus scrofa (Pig) [TaxID: 9823]
Phacochoerus africanus (Warthog) [TaxID: 41426]
Phacochoerus aethiopicus (Warthog) [TaxID: 85517]
Potamochoerus larvatus (Bushpig) [TaxID: 273792]

Protein attributes

Sequence length	119 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	FAD-dependent sulfhydryl oxidase that catalyzes disulfide bond formation. Involved in virion maturation and virulence By similarity. Ref.1
Catalytic activity	2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2.
Cofactor	FAD By similarity.
Subunit structure	Interacts with A151R By similarity.
Subcellular location	Host cytoplasm By similarity.
Induction	Expressed late in the infection cycle.
Sequence similarities	Belongs to the asfivirus B119L family. Contains 1 ERV/ALR sulfhydryl oxidase domain.

Ontologies

Keywords
Biological process	Virulence
Cellular component	Host cytoplasm
Developmental stage	Late protein
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thiol oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 119	119	FAD-linked sulfhydryl oxidase		PRO_0000355536
Regions
	Domain	1 – 97	97	ERV/ALR sulfhydryl oxidase
Amino acid modifications
	Disulfide bond	44 ↔ 47		Redox-active Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9JFM9 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: F6694FFC67A282E2	FASTA	119	14,379
	10 20 30 40 50 60 MLHWGPKFWR TLHLYAIFFS DTPGWKEKYE AIQWILNFIE SLPCTMCRHH AFSYLTKNPL 70 80 90 100 110 TLNNSEDFQY WTFAFHNNVN KRLNKKIISW SEYKNIYEQS ILNTIEYGKT DFIGAWSSL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An African swine fever virus ERV1-ALR homologue, 9GL, affects virion maturation and viral growth in macrophages and viral virulence in swine." Lewis T., Zsak L., Burrage T.G., Lu Z., Kutish G.F., Neilan J.G., Rock D.L. J. Virol. 74:1275-1285(2000) [PubMed: 10627538] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]	"Nucleotide sequence and analysis of 16.25 kilobase pairs of the African swine fever virus genome that span the central variable region." Roberts P.C., Lu Z., Rock D.L. Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"African swine fever virus genomes." Kutish G.F., Rock D.L. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF081174 Genomic DNA. Translation: AAF27970.1. L00966 Genomic DNA. Translation: AAL31324.1. AY261361 Genomic DNA. No translation available.
3D structure databases
ProteinModelPortal	Q9JFM9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR017905. ERV/ALR_sulphydryl_oxidase. IPR006863. Evr1_Alr. [Graphical view]
Gene3D	G3DSA:1.20.120.310. Evr1_Alr. 1 hit.
Pfam	PF04777. Evr1_Alr. 1 hit. [Graphical view]
SUPFAM	SSF69000. Evr1_Alr. 1 hit.
PROSITE	PS51324. ERV_ALR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FLSO_ASFM2

Accession

Primary (citable) accession number: Q9JFM9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	October 1, 2000
Last modified:	October 19, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program