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Q9JFB7

- KITH_VACCT

UniProt

Q9JFB7 - KITH_VACCT

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Protein

Thymidine kinase

Gene
TK, TJ2R
Organism
Vaccinia virus (strain Tian Tan) (VACV)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Phosphorylates thymidine and thymidine analogs, such as azidothymidine (AZT). Part of the salvage pathway for pyrimidine deoxyribonucleotide synthesis By similarity.

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei83 – 831Proton acceptor Reviewed prediction
Binding sitei113 – 1131Substrate; via amide nitrogen By similarity
Metal bindingi138 – 1381Zinc By similarity
Metal bindingi141 – 1411Zinc By similarity
Metal bindingi170 – 1701Zinc By similarity
Metal bindingi173 – 1731Zinc By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 188ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. thymidine kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinase (EC:2.7.1.21)
Gene namesi
Name:TK
ORF Names:TJ2R
OrganismiVaccinia virus (strain Tian Tan) (VACV)
Taxonomic identifieri10253 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
Virus hostiHomo sapiens (Human) [TaxID: 9606]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 177177Thymidine kinasePRO_0000174939Add
BLAST

Interactioni

Subunit structurei

Homotetramer By similarity. Two molecules of substrate bind to each enzyme tetramer By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ9JFB7.
SMRiQ9JFB7. Positions 4-175.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni157 – 1615Substrate binding By similarity

Sequence similaritiesi

Belongs to the thymidine kinase family.

Family and domain databases

InterProiIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view]
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiPF00265. TK. 1 hit.
[Graphical view]
PIRSFiPIRSF035805. TK_cell. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JFB7-1 [UniParc]FASTAAdd to Basket

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MNGGHIQLMI GPMFSGKSTE LIRRVRRYQI AQYKCVTIKY SNDNRYGTGL    50
WTHDKNNFEA LEATKLCDVL ESITDFSVIG IDEGQFFPDI VEFCERMANE 100
GKIVIVAALD GTFQRKPFNN ILNLIPLSEM VVKLTAVCMK CFKEASFSKR 150
LGEETEIEII GGNDMYQSVC RKCYIDS 177
Length:177
Mass (Da):20,118
Last modified:October 1, 2000 - v1
Checksum:i480CE5FE6A3B3911
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF095689 Genomic DNA. Translation: AAF33953.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF095689 Genomic DNA. Translation: AAF33953.1 .

3D structure databases

ProteinModelPortali Q9JFB7.
SMRi Q9JFB7. Positions 4-175.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view ]
PANTHERi PTHR11441. PTHR11441. 1 hit.
Pfami PF00265. TK. 1 hit.
[Graphical view ]
PIRSFi PIRSF035805. TK_cell. 1 hit.
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genomic sequence of vaccinia virus (Tian Tan strain)."
    Jin Q., Hou Y.D., Cheng N.H., Yao E.M., Cheng S.X., Yang X.K., Jing D.Y., Yu W.H., Yuan J.S., Ma X.J.
    Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiKITH_VACCT
AccessioniPrimary (citable) accession number: Q9JFB7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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