Q9JFA8 (MCEL_VACCT) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 30.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: mRNA-capping enzyme catalytic subunit Alternative name(s): Virus termination factor large subunit Short name=VTF large subunit mRNA-capping enzyme 97 kDa subunit mRNA-capping enzyme D1 subunit mRNA-capping enzyme large subunit Including the following 3 domains: | ||
| Gene names |
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| Organism | Vaccinia virus (strain Tian Tan) (VACV) | ||
| Taxonomic identifier | 10253 [NCBI] | ||
| Taxonomic lineage | Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Orthopoxvirus › Vaccinia virus | ||
| Virus host | Homo sapiens (Human) [TaxID: 9606] |
Protein attributes
| Sequence length | 844 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Function | Catalytic subunit of the mRNA capping enzyme which catalyzes three enzymatic reactions: the 5' triphosphate end of the pre-mRNA is hydrolyzed to a diphosphate by RNA 5' triphosphatase; the diphosphate RNA end is capped with GMP by RNA guanylyltransferase and the GpppN cap is methylated by RNA (guanine-N7) methyltransferase. Heterodimeric mRNA capping enzyme catalyzes the linkage of a N7-methyl-guanosine moiety to the first transcribed nucleotide (cap 0 structure), whereas the polymerase associated VP39 is responsible for a second methylation at the 2'-O position of the ribose (cap 1 structure) By similarity. The heterodimeric enzyme is also involved in early viral gene transcription termination and intermediate viral gene transcription initiation. Early gene transcription termination requires the termination factor VTF, the DNA-dependent ATPase NPH-I and the Rap94 subunit of the viral RNA polymerase, as well as the presence of a specific termination motif. Binds, together with RAP94, to the termination motif 5'-UUUUUNU-3' in the nascent early mRNA By similarity. |
| Catalytic activity | A 5'-phosphopolynucleotide + H2O = a polynucleotide + phosphate. GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA. S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA. |
| Subunit structure | Heterodimer of a catalytic and a regulatory subunit. Intrinsic methyltransferase activity of the catalytic subunit is weak and needs to be stimulated 30- to 50-fold by the regulatory subunit, which is itself catalytically inert By similarity. |
| Subcellular location | Virion Probable. Note: All the enzymes and other proteins required to synthesize early mRNAs are packaged within the virion core along with the DNA genome. |
| Domain | The N-terminus contains the triphosphatase and guanylyltransferase domains, whereas the C-terminus contains the methyltransferase domain. The N-terminus is involved in binding to the temination motif 5'-UUUUUNU-3' in the nascent mRNA By similarity. |
| Sequence similarities | Belongs to the viral GTase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | mRNA capping mRNA processing |
| Cellular component | Virion |
| Ligand | GTP-binding Metal-binding Nucleotide-binding S-adenosyl-L-methionine |
| Molecular function | Hydrolase Methyltransferase Nucleotidyltransferase Transferase |
| Technical term | Multifunctional enzyme |
| Gene Ontology (GO) | |
| Biological process | mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | virion Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | GTP binding Inferred from electronic annotation. Source: UniProtKB-KW mRNA (guanine-N7-)-methyltransferase activityInferred from electronic annotation. Source: EC mRNA guanylyltransferase activityInferred from electronic annotation. Source: EC metal ion bindingInferred from electronic annotation. Source: UniProtKB-KW polynucleotide 5'-phosphatase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 844 | 844 | mRNA-capping enzyme catalytic subunit | PRO_0000210123 | |||||
Regions | |||||||||
| Region | 1 – 539 | 539 | Triphosphatase-guanylyltransferase By similarity | ||||||
| Region | 540 – 844 | 305 | Methyltransferase By similarity | ||||||
Sites | |||||||||
| Active site | 260 | 1 | N6-GMP-lysine intermediate By similarity | ||||||
| Metal binding | 37 | 1 | Catalytic; for RNA triphosphatase activity Potential | ||||||
| Metal binding | 39 | 1 | Catalytic; for RNA triphosphatase activity Potential | ||||||
| Metal binding | 192 | 1 | Catalytic; for RNA triphosphatase activity Potential | ||||||
| Metal binding | 194 | 1 | Catalytic; for RNA triphosphatase activity Potential | ||||||
| Site | 77 | 1 | Essential for RNA triphosphatase activity By similarity | ||||||
| Site | 107 | 1 | Essential for RNA triphosphatase activity By similarity | ||||||
| Site | 126 | 1 | Essential for RNA triphosphatase activity By similarity | ||||||
| Site | 159 | 1 | Essential for RNA triphosphatase activity By similarity | ||||||
| Site | 161 | 1 | Essential for RNA triphosphatase activity By similarity | ||||||
Sequences
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References
| [1] | "Complete genomic sequence of vaccinia virus (Tian Tan strain)." Jin Q., Hou Y.D., Cheng N.H., Yao E.M., Cheng S.X., Yang X.K., Jing D.Y., Yu W.H., Yuan J.S., Ma X.J. Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AF095689 Genomic DNA. Translation: AAF33967.1. |
3D structure databases | |
| ProteinModelPortal | Q9JFA8. |
| SMR | Q9JFA8. Positions 545-844. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Family and domain databases | |
| InterPro | IPR019602. mRNA_cap_ATPase/GuylTrfase_vir. IPR004971. Pox_MCEL. [Graphical view] |
| Pfam | PF10640. Pox_ATPase-GT. 1 hit. PF03291. Pox_MCEL. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | MCEL_VACCT | ||||||||
| Accession | Primary (citable) accession number: Q9JFA8 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Viral Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |

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