Q9J5B8 (PAP1_FOWPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Poly(A) polymerase catalytic subunit
EC=2.7.7.19

Alternative name(s):
Poly(A) polymerase large subunit
Short name=PAP-L
VP55

Gene names

Name:	PAPL
Ordered Locus Names:	FPV102

Organism

Fowlpox virus (strain NVSL) (FPV) [Reference proteome]

Taxonomic identifier

928301 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Avipoxvirus › Fowlpox virus

Virus host

Vertebrata [TaxID: 7742]

Protein attributes

Sequence length	472 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Polymerase that creates the 3'-poly(A) tail of mRNA's.
Catalytic activity	ATP + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Heterodimer of a large (catalytic) subunit and a small (regulatory) subunit By similarity.
Sequence similarities	Belongs to the poxviridae poly(A) polymerase catalytic subunit family.

Ontologies

Keywords
Biological process	Transcription mRNA processing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW polynucleotide adenylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 472	472	Poly(A) polymerase catalytic subunit		PRO_0000099109
Sites
	Active site	194	1	By similarity
	Active site	196	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9J5B8 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: ADDD6F39EC84DC22	FASTA	472	56,081
	10 20 30 40 50 60 MERSRQVYYI INEYLGRHPS STEYQVLKHQ VEKISKINNF NKETFFFLLK KNKNKFFKDL 70 80 90 100 110 120 ELSDDLLKKR IDEYFSKQKH AKRLGNLFAI MELQKILISS FTKTIGILTT KVPEYYHSTI 130 140 150 160 170 180 KLEYSSMEKI ADDILDSYNV VEPSKEVKGR HKVSDLVGHV YKIMEEYLRR HSNSCLCYGS 190 200 210 220 230 240 YSLHFLNNKI EYGDIDVLQT NARTFLINIA FLIKFITGRR IVLLKVPFLK NYVIMHDEET 250 260 270 280 290 300 NHVMDTFNIR EKTMNMIPKI MIDNMYIVDP CIQLLNMIKM LSQIDRLEEL QAKFEKLSVR 310 320 330 340 350 360 LGTLLEYTRY RYSIPLDSES ILEVRAKLDK DKRKITVDFK KYKLNYIKCY FYLDEVELKK 370 380 390 400 410 420 FISKNSGLDE YEDFEAVTNS EYAIRNKTMY TYFSNTALMR SENEIHPITI NALTSHALLY 430 440 450 460 470 HVITRKFYDD LLGDLVRSLM IVEKVPVFKI IPRDKKQGRH TIIDIEKDII FH

References

[1]	"The genome of fowlpox virus." Afonso C.L., Tulman E.R., Lu Z., Zsak L., Kutish G.F., Rock D.L. J. Virol. 74:3815-3831(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Comparison of the genome sequence of FP9, an attenuated, tissue culture-adapted European fowlpox virus, with those of virulent American and European viruses." Skinner M.A., Laidlaw S.M. J. Gen. Virol. 85:305-322(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HP1-438 Munich.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF198100 Genomic DNA. Translation: AAF44446.1. AJ581527 Genomic DNA. Translation: CAE52643.1.
RefSeq	NP_039065.1. NC_002188.1.
3D structure databases
ProteinModelPortal	Q9J5B8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1486650.
Phylogenomic databases
ProtClustDB	PHA2996.
Family and domain databases
InterPro	IPR024231. Pox_polyA_pol_nucTrfase. IPR004976. Poxvirus_polyA_pol_cat. IPR024397. Poxvirus_polyA_pol_cat_C. IPR024398. Poxvirus_polyA_pol_cat_N. [Graphical view]
Pfam	PF03296. Pox_polyA_pol. 1 hit. PF12629. Pox_polyA_pol_C. 1 hit. PF12630. Pox_polyA_pol_N. 1 hit. [Graphical view]
PIRSF	PIRSF015693. VAC-48L_nuct. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PAP1_FOWPN

Accession

Primary (citable) accession number: Q9J5B8
Secondary accession number(s): Q70H53

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2001
Last sequence update:	October 1, 2000
Last modified:	April 3, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program