Reviewed, UniProtKB/Swiss-Prot Q9J584 (MCEL_FOWPV)

Names and origin

Protein names

Recommended name:
    mRNA-capping enzyme large subunit
Including the following 2 domains:
    1- Recommended name:
            Polynucleotide 5'-triphosphatase
              EC=3.1.3.33
        Alternative name(s):
            mRNA 5'-triphosphatase
              Short name=TPase
    2- Recommended name:
            mRNA guanylyltransferase
              EC=2.7.7.50
        Alternative name(s):
            GTP--RNA guanylyltransferase
              Short name=GTase

Gene names

Ordered Locus Names:

FPV146

Organism

Fowlpox virus (FPV)

Taxonomic identifier

10261 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Avipoxvirus

Virus host

Vertebrata [TaxID: 7742]

Protein attributes

Sequence length	851 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the first two reactions in the mRNA cap formation pathway.
Catalytic activity	A 5'-phosphopolynucleotide + H2O = a polynucleotide + phosphate. GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.
Subunit structure	Heterodimer of a large and a small subunit By similarity.
Sequence similarities	Belongs to the viral GTase family.

Ontologies

Keywords
Biological process	mRNA capping mRNA processing
Ligand	GTP-binding Nucleotide-binding
Molecular function	Hydrolase Nucleotidyltransferase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW mRNA guanylyltransferase activity Inferred from electronic annotation. Source: EC polynucleotide 5'-phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 851	851	mRNA-capping enzyme large subunit		PRO_0000210130
Sites
	Active site	266	1	N6-GMP-lysine intermediate Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9J584-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: DC3607ADD634BA7A	FASTA	851	98,888
	10 20 30 40 50 60 MDKYISKTPL SCYFEELVDT FISVVNSINK VDESKHHEVE LILFKPPIIT LTNLYNMATT 70 80 90 100 110 120 TESYIEFTML PVDKPNTKFR NRIPLSKIHG LDVKNNQLVE SLDGFIWEEK SLLLKKDISD 130 140 150 160 170 180 NSSAIIKYSI EEKTLFVDYK RRNASIKLEL VSVVRAKLRN IVIDFKMKYF LGSGAQSANS 190 200 210 220 230 240 SSLLCALNHP KNKPSLYIEF EIMMQDKNIS KKKLLEELNM SASALFLSHP KYIRLCPSIN 250 260 270 280 290 300 PILRTHLLKK QDIININTDD LYITSKTDGI FSHVYIEKKS IFCYFSHLGY IKEYTASREI 310 320 330 340 350 360 EETIYLYAEM RKEESILYLT VIKVLKPCME DRLSELAFVK NHLTGIHDRL VFVTKCYDGP 370 380 390 400 410 420 FESSSDLVVS IEEMLKTEQE GIILFYSKGE DSTTDYKVKK DNTIDQCVNV IYRYMSSEPI 430 440 450 460 470 480 VFNDKGSFLE YKRYSNDKGF PKEFSTGKLD LNGSVEYINN IYCIEIKHLN PCTGITNLVL 490 500 510 520 530 540 PIKFIAEFSH NDELIQPRID KTMKYLYESG YYGNQLSVIM DHLNDQKLRI GDVFEEEKLA 550 560 570 580 590 600 DIAAHMKLKD SMRLNPDGNY FLSNRVRGAL GILSNFVKTL LISLYCSKTY LDNHSKRKVL 610 620 630 640 650 660 AIDFGNGADL EKYFYGEIAL MVATDPDDNA IETGKKRYNE RNAGDKSKYY KFNYIKETIR 670 680 690 700 710 720 SETYVSSIRQ VLYFEKFSLV DWQFAIHYSF HPKHYSTIMT NLQELTESGC KVLITTMDGD 730 740 750 760 770 780 YLDTLKEKKK FIIRKLLPET ENYLSIEKID DDKVLVYNPS SMSKPMAEYI VRRDTLIRVF 790 800 810 820 830 840 REYKFKLIDS CNFKTIIDRN ISFINGVSRL ESRGSTKNFF ELNRKALEEC NDTDVLELLS 850 HYMVYVFSKE V

References

[1]	"The genome of fowlpox virus." Afonso C.L., Tulman E.R., Lu Z., Zsak L., Kutish G.F., Rock D.L. J. Virol. 74:3815-3831(2000) [PubMed: 10729156] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF198100 Genomic DNA. Translation: AAF44490.1.
RefSeq	NP_039109.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1486694.
Enzyme and pathway databases
BRENDA	2.7.7.50. 288568. 3.1.3.33. 288568.
Family and domain databases
InterPro	IPR019602. mRNA_cap_ATPase/GuylTrfase_vir. IPR004971. Pox_MCEL. [Graphical view]
Pfam	PF10640. Pox_ATPase-GT. 1 hit. PF03291. Pox_MCEL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MCEL_FOWPV

Accession

Primary (citable) accession number: Q9J584

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2001
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)