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Protein

Envelope glycoprotein E

Gene

gE

Organism
Varicella-zoster virus (strain Oka vaccine) (HHV-3) (Human herpesvirus 3)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Envelope glycoprotein that binds to the potential host cell entry receptor IDE.
In epithelial cells, the heterodimer gE/gI is required for the cell-to-cell spread of the virus, by sorting nascent virions to cell junctions. Once the virus reaches the cell junctions, virus particles can spread to adjacent cells extremely rapidly through interactions with cellular receptors that accumulate at these junctions. Implicated in basolateral spread in polarized cells. In neuronal cells, gE/gI is essential for the anterograde spread of the infection throughout the host nervous system. Together with US9, the heterodimer gE/gI is involved in the sorting and transport of viral structural components toward axon tips (By similarity).By similarity
The heterodimer gE/gI serves as a receptor for the Fc part of host IgG. Dissociation of gE/gI from IgG occurs at acidic pH. May thus be involved in anti-VZV antibodies bipolar bridging, followed by intracellular endocytosis and degradation, thereby interfering with host IgG-mediated immune responses (By similarity).By similarity

GO - Molecular functioni

  • enzyme binding Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processHost-virus interaction, Viral attachment to host cell, Viral attachment to host entry receptor, Viral immunoevasion, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Envelope glycoprotein E
Short name:
gE
Gene namesi
Name:gE
ORF Names:ORF68
OrganismiVaricella-zoster virus (strain Oka vaccine) (HHV-3) (Human herpesvirus 3)
Taxonomic identifieri341980 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeVaricellovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000008505 Componenti: Genome
  • UP000008504 Componenti: Genome
  • UP000002603 Componenti: Genome
  • UP000008506 Componenti: Genome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini31 – 538Virion surfaceSequence analysisAdd BLAST508
Transmembranei539 – 559HelicalSequence analysisAdd BLAST21
Topological domaini560 – 623IntravirionSequence analysisAdd BLAST64

GO - Cellular componenti

Keywords - Cellular componenti

Host cell junction, Host cell membrane, Host endosome, Host Golgi apparatus, Host membrane, Membrane, Viral envelope protein, Virion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi593 – 598SESTDT → AESADA: Complete loss of phosphorylation by ORF47 kinase. 1 Publication6

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30Sequence analysisAdd BLAST30
ChainiPRO_000038549931 – 623Envelope glycoprotein EAdd BLAST593

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi266N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Disulfide bondi387 ↔ 413By similarity
Disulfide bondi396 ↔ 405By similarity
Disulfide bondi432 ↔ 442By similarity
Glycosylationi437N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Modified residuei438Sulfotyrosine; by hostSequence analysis1
Modified residuei441Sulfotyrosine; by hostSequence analysis1
Modified residuei593PhosphoserineBy similarity1
Modified residuei595PhosphoserineBy similarity1
Modified residuei596PhosphothreonineBy similarity1
Modified residuei598PhosphothreonineBy similarity1

Post-translational modificationi

Phosphorylated on serines within the acidic cluster. Phosphorylation determines whether endocytosed viral gE traffics to the trans-Golgi network or recycles to the cell membrane.Curated

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein, Sulfation

Interactioni

Subunit structurei

Interacts (via N-terminus) with host receptor IDE (via N-terminus). Interacts with gI; this interaction enhances the Fc receptor function of gE (By similarity). The heterodimer gE/gI interacts with the Fc part of host IgG (By similarity).By similarity

GO - Molecular functioni

  • enzyme binding Source: UniProtKB

Protein-protein interaction databases

DIPiDIP-56349N.
ELMiQ9J3M8.
IntActiQ9J3M8. 17 interactors.

Structurei

3D structure databases

SMRiQ9J3M8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni208 – 236Interaction with gIAdd BLAST29
Regioni352 – 499Fc-bindingBy similarityAdd BLAST148
Regioni588 – 601AcidicAdd BLAST14

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi582 – 585Internalization motifSequence analysis4

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

OrthoDBiVOG0900004S.

Family and domain databases

InterProiView protein in InterPro
IPR003404. Herpes_glycopE.
IPR007110. Ig-like_dom.
PfamiView protein in Pfam
PF02480. Herpes_gE. 1 hit.
SUPFAMiSSF48726. SSF48726. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9J3M8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGTVNKPVVG VLMGFGIITG TLRITNPVRA SVLRYDDFHI DEDKLDTNSV
60 70 80 90 100
YEPYYHSDHA ESSWVNRGES SRKAYDHNSP YIWPRNDYDG FLENAHEHHG
110 120 130 140 150
VYNQGRGIDS GERLMQPTQM SAQEDLGDDT GIHVIPTLNG DDRHKIVNVD
160 170 180 190 200
QRQYGDVFKG DLNPKPQGQR LIEVSVEENH PFTLRAPIQR IYGVRYTETW
210 220 230 240 250
SFLPSLTCTG DAAPAIQHIC LKHTTCFQDV VVDVDCAENT KEDQLAEISY
260 270 280 290 300
RFQGKKEADQ PWIVVNTSTL FDELELDPPE IEPGVLKVLR TEKQYLGVYI
310 320 330 340 350
WNMRGSDGTS TYATFLVTWK GDEKTRNPTP AVTPQPRGAE FHMWNYHSHV
360 370 380 390 400
FSVGDTFSLA MHLQYKIHEA PFDLLLEWLY VPIDPTCQPM RLYSTCLYHP
410 420 430 440 450
NAPQCLSHMN SGCTFTSPHL AQRVASTVYQ NCEHADNYTA YCLGISHMEP
460 470 480 490 500
SFGLILHDGG TTLKFVDTPE SLSGLYVFVV YFNGHVEAVA YTVVSTVDHF
510 520 530 540 550
VNAIEERGFP PTAGQPPATT KPKEITPVNP GTSPLLRYAA WTGGLAAVVL
560 570 580 590 600
LCLVIFLICT AKRMRVKAYR VDKSPYNQSM YYAGLPVDDF EDSESTDTEE
610 620
EFGNAIGGSH GGSSYTVYID KTR
Length:623
Mass (Da):69,968
Last modified:October 1, 2000 - v1
Checksum:iCFC5EF31E4B7D31F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF206304 Genomic DNA. Translation: AAF61669.1.
AY016450 Genomic DNA. Translation: AAK19946.1.
AB097932 Genomic DNA. No translation available.
AB097933 Genomic DNA. No translation available.
DQ008354 Genomic DNA. Translation: AAY57677.1.
DQ008355 Genomic DNA. Translation: AAY57748.1.

Similar proteinsi

Entry informationi

Entry nameiGE_VZVO
AccessioniPrimary (citable) accession number: Q9J3M8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: October 1, 2000
Last modified: August 30, 2017
This is version 66 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families