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Q9IZU7 (VP2_CAVCA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 29. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Dual specificity protein phosphatase VP2
EC=3.1.3.16
EC=3.1.3.48
Gene names
Name:VP2
OrganismChicken anemia virus (isolate Australia/CAU269-7/2000) (CAV) [Complete proteome]
Taxonomic identifier486492 [NCBI]
Taxonomic lineageViruses › ssDNA viruses › Circoviridae › Gyrovirus ›
Virus hostGallus gallus (Chicken) [TaxID: 9031]

Protein attributes

Sequence length216 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May act as a scaffold protein in virion assembly. May also play a role in intracellular signaling during viral replication. Ref.2

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

A phosphoprotein + H2O = a protein + phosphate.

Induction

VP1 and VP2 are detected 12 hours post infection, while VP3 only after 24 hours.

Sequence similarities

Belongs to the gyrovirus protein VP2 family.

Ontologies

Keywords
   Developmental stageEarly protein
   Molecular functionHydrolase
Protein phosphatase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processpeptidyl-tyrosine dephosphorylation

Inferred from electronic annotation. Source: GOC

   Molecular_functionprotein tyrosine phosphatase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 216216Dual specificity protein phosphatase VP2
PRO_0000314474

Sites

Active site951Phosphocysteine intermediate Probable

Experimental info

Mutagenesis951C → S: Complete loss of phosphatase activity. Reduced cytopathogenicity and marked inhibition of viral growth in cell culture. Ref.2
Mutagenesis971C → S: 70% loss of phosphotyrosine activity. 700% increase of phosphoserine/phosphothreonine phosphatase activity. Reduced cytopathogenicity and marked inhibition of viral growth in cell culture. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q9IZU7 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: F83EB3D07B97F752

FASTA21624,112
        10         20         30         40         50         60 
MHGNGGQPAA GGSESALSRE GQPGPSGAAQ GQVISNERSP RRYSTRTING VQATNKFTAV 

        70         80         90        100        110        120 
GNPSLQRDPD WYRWNYSHSI AVWLRECSRS HAKICNCGQF RKHWFQECAG LEDRSTQASL 

       130        140        150        160        170        180 
EEAILRPLRV QGKRAKRKLD YHYSQPTPNR KKVYKTVRWQ DELADREADF TPSEEDGGTT 

       190        200        210 
SSDFDEDINF DIGGDSGIVD ELLGRPFTTP APVRIV

« Hide

References

[1]"Full-length infectious clone of a pathogenic Australian isolate of chicken anaemia virus."
Brown H.K., Browning G.F., Scott P.C., Crabb B.S., Brown K.
Aust. Vet. J. 78:637-640(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Chicken anemia virus VP2 is a novel dual specificity protein phosphatase."
Peters M.A., Jackson D.C., Crabb B.S., Browning G.F.
J. Biol. Chem. 277:39566-39573(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF CYS-95.
[3]"Mutation of chicken anemia virus VP2 differentially affects serine/threonine and tyrosine protein phosphatase activities."
Peters M.A., Jackson D.C., Crabb B.S., Browning G.F.
J. Gen. Virol. 86:623-630(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF CYS-97.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF227982 Genomic DNA. Translation: AAF34787.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR004118. HEV_TT_virus_Orf2/Gyrovir_Vp2.
[Graphical view]
PfamPF02957. TT_ORF2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameVP2_CAVCA
AccessionPrimary (citable) accession number: Q9IZU7
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 1, 2000
Last modified: April 3, 2013
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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