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Q9IZK2

- GCNT3_BHV4V

UniProt

Q9IZK2 - GCNT3_BHV4V

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Protein

Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase

Gene

Bo17

Organism
Bovine herpesvirus 4 (strain V. test) (BoHV-4) (Movar virus)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Non-essential glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans. Contributes to the post-translational modifications of structural proteins.2 Publications

Catalytic activityi

UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R = UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R.1 Publication
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R.1 Publication

Pathwayi

GO - Molecular functioni

  1. beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity Source: UniProtKB-EC
  2. N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.1.102. 923.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT14. Glycosyltransferase Family 14.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase (EC:2.4.1.102, EC:2.4.1.150)
Alternative name(s):
BORFF3-4
C2GnT-mucin type
Short name:
C2GnT-M
Gene namesi
Name:Bo17
OrganismiBovine herpesvirus 4 (strain V. test) (BoHV-4) (Movar virus)
Taxonomic identifieri436507 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeRhadinovirus
Virus hostiBos taurus (Bovine) [TaxID: 9913]

Subcellular locationi

GO - Cellular componenti

  1. host cell Golgi apparatus Source: UniProtKB-KW
  2. host cell membrane Source: UniProtKB-KW
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host Golgi apparatus, Host membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 440440Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferasePRO_0000288554Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi72 – 721N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi73 ↔ 230By similarity
Glycosylationi108 – 1081N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi164 ↔ 384By similarity
Disulfide bondi185 ↔ 212By similarity
Disulfide bondi393 ↔ 425By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Developmental stagei

Expressed during BHV-4 repkication (at protein level).2 Publications

Structurei

3D structure databases

ProteinModelPortaliQ9IZK2.
SMRiQ9IZK2. Positions 70-435.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 99CytoplasmicSequence Analysis
Topological domaini31 – 440410LumenalSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei10 – 3021Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 14 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR003406. Glyco_trans_14.
[Graphical view]
PfamiPF02485. Branch. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9IZK2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKMAGWKKKL CPGHHLWALG CYMLLAVVSL RLSLRFKCDV DSLDLESRDF
60 70 80 90 100
QSQHCRDMLY NSLKLPAKRS INCSGITRGD QEAVVQALLD NLEVKKKRPP
110 120 130 140 150
LTDTYYLNIT RDCERFKAQR KFIQFPLSKE ELDFPIAYSM VVHEKIENFE
160 170 180 190 200
RLLRAVYAPQ NIYCVHVDVK SPETFKEAVK AIISCFPNVF MASKLVPVVY
210 220 230 240 250
ASWSRVQADL NCMEDLLQSS VSWKYLLNTC GTDFPIKTNA EMVLALKMLK
260 270 280 290 300
GKNSMESEVP SESKKNRWKY RYEVTDTLYP TSKIKDPPPD NLPMFTGNAY
310 320 330 340 350
FVASRAFVQH VLDNPKSQIL VEWVKDTYSP DEHLWATLQR APWMPGSVPS
360 370 380 390 400
HPKYHISDMT AIARLVKWQY HEGDVSMGAP YAPCSGIHRR AICIYGAGDL
410 420 430 440
YWILQNHHLL ANKFDPRVDD NVLQCLEEYL RHKAIYGTEL
Length:440
Mass (Da):50,702
Last modified:October 1, 2000 - v1
Checksum:i71B9C3B6B4A949BD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF231105 Genomic DNA. Translation: AAF72001.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF231105 Genomic DNA. Translation: AAF72001.1 .

3D structure databases

ProteinModelPortali Q9IZK2.
SMRi Q9IZK2. Positions 70-435.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GT14. Glycosyltransferase Family 14.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00378 .
BRENDAi 2.4.1.102. 923.

Family and domain databases

InterProi IPR003406. Glyco_trans_14.
[Graphical view ]
Pfami PF02485. Branch. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, ENZYME ACTIVITY, DEVELOPMENTAL STAGE.
  2. "The core 2 beta-1,6-N-acetylglucosaminyltransferase-M encoded by bovine herpesvirus 4 is not essential for virus replication despite contributing to post-translational modifications of structural proteins."
    Markine-Goriaynoff N., Gillet L., Karlsen O.A., Haarr L., Minner F., Pastoret P.-P., Fukuda M., Vanderplasschen A.
    J. Gen. Virol. 85:355-367(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiGCNT3_BHV4V
AccessioniPrimary (citable) accession number: Q9IZK2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: October 1, 2000
Last modified: October 29, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Was acquired from an ancestor of the African buffalo around 1.5 million years ago.

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3