ID   RDRP_POLVP              Reviewed;         739 AA.
AC   Q9IWA0; Q9QBU4;
DT   05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   13-SEP-2023, entry version 73.
DE   RecName: Full=RNA-directed RNA polymerase;
DE            EC=2.7.7.48;
DE   AltName: Full=Protein p84;
DE   Contains:
DE     RecName: Full=Protein p25;
GN   ORFNames=ORF1;
OS   Pothos latent virus (isolate Pigeonpea/India) (PoLV).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes;
OC   Tolivirales; Tombusviridae; Procedovirinae; Aureusvirus; Aureusvirus aurei.
OX   NCBI_TaxID=652109;
OH   NCBI_TaxID=3821; Cajanus cajan (Pigeon pea) (Cajanus indicus).
OH   NCBI_TaxID=174212; Pothos.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   AGRICOLA=IND23224278;
RA   Lava Kumar P., Jones A.T., Sreenivasulu P., Fenton B., Reddy D.V.R.;
RT   "Characterization of a virus from pigeonpea with affinities to species in
RT   the genus Aureusvirus, family Tombusviridae.";
RL   Plant Dis. 85:208-215(2001).
CC   -!- FUNCTION: RNA-dependent RNA polymerase that plays an essential role in
CC       the virus replication. {ECO:0000305}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- MISCELLANEOUS: Readthrough of the terminator UAG occurs at position
CC       223.
CC   -!- SIMILARITY: Belongs to the tombusviridae RNA polymerase family.
CC       {ECO:0000305}.
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DR   EMBL; AJ243370; CAB59792.1; -; Genomic_RNA.
DR   EMBL; AJ243370; CAB59793.1; -; Genomic_RNA.
DR   Proteomes; UP000000572; Genome.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   CDD; cd23243; Aureusvirus_RdRp; 1.
DR   Gene3D; 3.30.70.270; -; 1.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR   InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR   InterPro; IPR002166; RNA_pol_HCV.
DR   InterPro; IPR013707; Tombusvirus_p33.
DR   Pfam; PF00998; RdRP_3; 1.
DR   Pfam; PF08500; Tombus_P33; 1.
DR   SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   3: Inferred from homology;
KW   Nucleotide-binding; Nucleotidyltransferase; Reference proteome;
KW   RNA suppression of termination; RNA-directed RNA polymerase; Transferase;
KW   Viral RNA replication.
FT   CHAIN           1..739
FT                   /note="RNA-directed RNA polymerase"
FT                   /id="PRO_0000399488"
FT   CHAIN           1..222
FT                   /note="Protein p25"
FT                   /id="PRO_0000399489"
FT   DOMAIN          452..567
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
SQ   SEQUENCE   739 AA;  83699 MW;  D70C31F915221506 CRC64;
     MANWCMSVLM FSFVTVYTIV TSLGQGIRRG FRAIVDEVEN IYGDMCAGFK VALSKLSVSW
     IVVVGMIFVL NSLVVGLVPT LTMVAIIAAC VGAKYGGRVP RYIRAHVDRI RKSWEKGVDD
     DDCCAPLPET SLERVPTKLR PRLACKIAVR AIAKVGLLKR SEANSMVYQR VCLDVMESMK
     MRWHDRLVIL PQAVLACLER PEEVEEVMKA IEASCKGRFD VYXGCLLQHR GYNTAIISAI
     PDGVLLNHEG MVVRKGAPIT TERKWYSFAG YASTYEYIVH NSSLVNVCRG LVERVFCVVR
     DGKLQRPLRP KNNVFERKLG NVGRRISSIV GYCPAMTRSE FCESYSGTRR ATYERARLSL
     DVLPCTRKDA YLKTFVKAEK INITLKPDPA PRVIQPRDPR YNVEVGRYLK PLEPRLMKAI
     DKLWGEKTAI KGYTVEKVGE ILHQKSLRFK NPCFVGLDAS RFDQHCSRQA LEWEHSVYNA
     IFRDPYLSEL LTWQIDNVGT AYLKDGFVRY RVDGCRMSGD MNTSMGNYLI MSCLVYQFCK
     DIGIDASLAN CGDDCVLYLE KEDLPKLKAL PDWFGKMGYS MKVEKPVFTV EEIEFCQQHP
     VQLSRGYVMV RRPDVCLTKD LCVVRGGMTT ERLQRWLYAQ HDGGLALTGD CPVLGAFYRR
     FPSGESHGEL SEYSDAHKFK AGKQYGEINS LARYSFWLAF GITPDEQLAI ERDLMNWTPN
     VVPGSCDPRP TLLDYCSDN
//