Q9IPJ8 (PHOSP_RABVN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoprotein
Short name=Protein P

Alternative name(s):
Protein M1

Gene names

Name:

P

Organism

Rabies virus (strain Nishigahara RCEH) (RABV) [Complete proteome]

Taxonomic identifier

11298 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA negative-strand viruses › Mononegavirales › Rhabdoviridae › Lyssavirus › Rabies virus

Virus host

Homo sapiens (Human) [TaxID: 9606]
Mammalia [TaxID: 40674]

Protein attributes

Sequence length	297 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Non catalytic polymerase cofactor and regulatory protein that plays a role in viral transcription and replication. Stabilizes the RNA polymerase L to the N-RNA template and binds the soluble protein N, preventing it from encapsidating non-genomic RNA. Also inhibits host IFN-alpha and IFN-beta signaling by binding and retaining phosphorylated STAT1 in the cytoplasm or by inhibiting the DNA binding of STAT1 in the nucleus. Might be involved, through interaction with host dynein, in intracellular microtubule-dependent virus transport of incoming virus from the synapse toward the cell body By similarity.
Subunit structure	Homotrimer when phosphorylated. This trimer is stabilized by binding to the L protein. Binds soluble protein N, and ribonucleocapsid. Interacts with host DYNLL1 and DYNLL2; this interaction may play a role in intracellular microtubule-dependent virus transport of incoming virus. Interacts with host STAT1, STAT2 and PML. Isoform P3 binds host PML By similarity.
Subcellular location	Phosphoprotein: Virion. Host cytoplasm By similarity. Isoform P2: Host cytoplasm By similarity. Isoform P3: Host nucleus By similarity. Isoform P4: Host nucleus By similarity. Isoform P5: Host nucleus By similarity.
Post-translational modification	Phosphorylated by host PKC and by an unknown kinase By similarity.
Sequence similarities	Belongs to the lyssavirus protein P family.

Ontologies

Keywords
Biological process	Cytoplasmic inwards viral transport Host-virus interaction Inhibition of host STAT1 by virus Inhibition of host STAT2 by virus Inhibition of host innate immune response by virus Inhibition of host interferon signaling pathway by virus Interferon antiviral system evasion Microtubular inwards viral transport Viral RNA replication Viral immunoevasion Virus entry into host cell
Cellular component	Host cytoplasm Host nucleus Virion
Coding sequence diversity	Alternative initiation
Molecular function	Chaperone
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	suppression by virus of host STAT1 activity Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host STAT2 activity Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host type I interferon-mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW viral entry into host cell Inferred from electronic annotation. Source: UniProtKB-KW viral transcription Inferred from electronic annotation. Source: InterPro
Cellular_component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative initiation. [Align] [Select]

Isoform P (identifier: Q9IPJ8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform P2 (identifier: Q9IPJ8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-19: Missing.

Isoform P3 (identifier: Q9IPJ8-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-52: Missing.

Isoform P4 (identifier: Q9IPJ8-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-68: Missing.

Isoform P5 (identifier: Q9IPJ8-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-82: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 297	297	Phosphoprotein		PRO_0000295254
Regions
	Region	138 – 172	35	DYNLL1 and DYNLL2 binding By similarity
	Motif	49 – 58	10	Nuclear export signal By similarity
	Motif	211 – 214	4	Nuclear localization signal By similarity
Amino acid modifications
	Modified residue	162	1	Phosphoserine; by host PKC By similarity
	Modified residue	210	1	Phosphoserine; by host PKC By similarity
	Modified residue	271	1	Phosphoserine; by host PKC By similarity
Natural variations
	Alternative sequence	1 – 82	82	Missing in isoform P5.		VSP_026913
	Alternative sequence	1 – 68	68	Missing in isoform P4.		VSP_026914
	Alternative sequence	1 – 52	52	Missing in isoform P3.		VSP_026915
	Alternative sequence	1 – 19	19	Missing in isoform P2.		VSP_026916
	Natural variant	15	1	L → M in strain: RC-HL.
	Natural variant	56 – 58	3	LHL → PHP in strain: Ni-CE.
	Natural variant	56	1	L → I in strain: RC-HL.
	Natural variant	60	1	D → Y in strain: RC-HL.
	Natural variant	66	1	L → P in strain: Ni-CE.
	Natural variant	81	1	F → P in strain: Ni-CE.
	Natural variant	100	1	V → I in strain: RC-HL.
	Natural variant	148	1	T → I in strain: RC-HL.
	Natural variant	165	1	K → A in strain: RC-HL.
	Natural variant	226	1	N → H in strain: Ni-CE.

Sequences

	Sequence		Length	Mass (Da)
	Isoform P [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 70F7184CC1B9C99C	FASTA	297	33,326
	10 20 30 40 50 60 MSKIFVNPSA IRAGLADLEM AEETVDLINR NIEDNQAHLQ GEPIEVDSLP EDMSRLHLDD 70 80 90 100 110 120 GKLPDLGRMS KAGEGRHQED FQMDEGEDPS LLFQSYLDNV GVQIVRQMRS GERFLKIWSQ 130 140 150 160 170 180 TVEEIISYVT VNFPNPSGRS SEDKSTQTTS QEPKKETTST PSQRKSQSLK SRTMAQTASG 190 200 210 220 230 240 PPSLEWSATN EEDDLSVEAE IAHQIAESFS KKYKFPSRSS GIFLYNFEQL KMNLDDIVKE 250 260 270 280 290 AKNVPGVTRL AHDGSKLPLR CVLGWVALAN SKKFQLLVEA NKLNKIMQDD LNRYASC
	Isoform P2 [UniParc]. Checksum: D560451C984C4386	FASTA	278	31,312
	10 20 30 40 50 60 MAEETVDLIN RNIEDNQAHL QGEPIEVDSL PEDMSRLHLD DGKLPDLGRM SKAGEGRHQE 70 80 90 100 110 120 DFQMDEGEDP SLLFQSYLDN VGVQIVRQMR SGERFLKIWS QTVEEIISYV TVNFPNPSGR 130 140 150 160 170 180 SSEDKSTQTT SQEPKKETTS TPSQRKSQSL KSRTMAQTAS GPPSLEWSAT NEEDDLSVEA 190 200 210 220 230 240 EIAHQIAESF SKKYKFPSRS SGIFLYNFEQ LKMNLDDIVK EAKNVPGVTR LAHDGSKLPL 250 260 270 RCVLGWVALA NSKKFQLLVE ANKLNKIMQD DLNRYASC
	Isoform P3 [UniParc]. Checksum: 5DF0DFEB270DAC3A	FASTA	245	27,595
	10 20 30 40 50 60 MSRLHLDDGK LPDLGRMSKA GEGRHQEDFQ MDEGEDPSLL FQSYLDNVGV QIVRQMRSGE 70 80 90 100 110 120 RFLKIWSQTV EEIISYVTVN FPNPSGRSSE DKSTQTTSQE PKKETTSTPS QRKSQSLKSR 130 140 150 160 170 180 TMAQTASGPP SLEWSATNEE DDLSVEAEIA HQIAESFSKK YKFPSRSSGI FLYNFEQLKM 190 200 210 220 230 240 NLDDIVKEAK NVPGVTRLAH DGSKLPLRCV LGWVALANSK KFQLLVEANK LNKIMQDDLN RYASC
	Isoform P4 [UniParc]. Checksum: B4EF215B8E3BCFAA	FASTA	229	25,790
	10 20 30 40 50 60 MSKAGEGRHQ EDFQMDEGED PSLLFQSYLD NVGVQIVRQM RSGERFLKIW SQTVEEIISY 70 80 90 100 110 120 VTVNFPNPSG RSSEDKSTQT TSQEPKKETT STPSQRKSQS LKSRTMAQTA SGPPSLEWSA 130 140 150 160 170 180 TNEEDDLSVE AEIAHQIAES FSKKYKFPSR SSGIFLYNFE QLKMNLDDIV KEAKNVPGVT 190 200 210 220 RLAHDGSKLP LRCVLGWVAL ANSKKFQLLV EANKLNKIMQ DDLNRYASC
	Isoform P5 [UniParc]. Checksum: 2A7FD6768298E1DE	FASTA	215	24,188
	10 20 30 40 50 60 MDEGEDPSLL FQSYLDNVGV QIVRQMRSGE RFLKIWSQTV EEIISYVTVN FPNPSGRSSE 70 80 90 100 110 120 DKSTQTTSQE PKKETTSTPS QRKSQSLKSR TMAQTASGPP SLEWSATNEE DDLSVEAEIA 130 140 150 160 170 180 HQIAESFSKK YKFPSRSSGI FLYNFEQLKM NLDDIVKEAK NVPGVTRLAH DGSKLPLRCV 190 200 210 LGWVALANSK KFQLLVEANK LNKIMQDDLN RYASC

References

[1]

"A comparison of complete genome sequences of the attenuated RC-HL strain of rabies virus used for production of animal vaccine in Japan, and the parental Nishigahara strain." Ito N., Kakemizu M., Ito K.A., Yamamoto A., Yoshida Y., Sugiyama M., Minamoto N. Microbiol. Immunol. 45:51-58(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Nishigahara and RC-HL.

[2]

"Involvement of nucleoprotein, phosphoprotein, and matrix protein genes of rabies virus in virulence for adult mice." Shimizu K., Ito N., Mita T., Yamada K., Hosokawa-Muto J., Sugiyama M., Minamoto N. Virus Res. 123:154-160(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Ni-CE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB044824 Genomic RNA. Translation: BAA96803.1. AB009663 Genomic RNA. Translation: BAA24084.1. AB128149 Genomic RNA. Translation: BAD04911.1.
PIR	B58460.
3D structure databases
HSSP	HSSP built from PDB template 1VYI based on UniProtKB P22363.
ProteinModelPortal	Q9IPJ8.
SMR	Q9IPJ8. Positions 186-296.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR004259. PP_M1. [Graphical view]
Pfam	PF03012. PP_M1. 1 hit. [Graphical view]
ProDom	PD004155. PP_M1. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

PHOSP_RABVN

Accession

Primary (citable) accession number: Q9IPJ8
Secondary accession number(s): O55595, Q75T11

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	October 1, 2000
Last modified:	April 3, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program