Q9IMM3 (B2_NODAM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein B2

Gene names

Name:

B2

Organism

Nodamura virus (strain Mag115) (NoV) [Reference proteome]

Taxonomic identifier

914672 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA positive-strand viruses, no DNA stage › Nodaviridae › Alphanodavirus › Nodamura virus

Virus host

Aedes [TaxID: 7158]
Lepidoptera (butterflies and moths) [TaxID: 7088]
Sus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length	137 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds double-strand RNA (dsRNA) with high affinity. Suppresses the host RNA silencing-based antiviral response. Ref.2
Subunit structure	Homodimer By similarity.
Domain	The dsRNA-binding region is required for suppression of RNA silencing By similarity.
Miscellaneous	Encoded on a subgenomic RNA (RNA3) synthesized during replication and which is co-terminal with RNA1.
Sequence similarities	Belongs to the nodaviridae B2 protein family.

Ontologies

Keywords
Coding sequence diversity	Alternative initiation
Ligand	RNA-binding
Molecular function	Suppressor of RNA silencing
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform 1 (identifier: Q9IMM3-1)

Also known as: B2/137;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9IMM3-2)

Also known as: B2/134;

The sequence of this isoform differs from the canonical sequence as follows:
     1-3: Missing.

Note: Produced by alternative initiation at Met-4 of isoform 1.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 137

137

Protein B2

PRO_0000402409

Regions

Region

1 – 79

79

RNA-binding

Natural variations

Alternative sequence

1 – 3

3

Missing in isoform 2.

VSP_040284

Secondary structure

1

.

.

.

.

.

.

.

.

.

137

Helix Strand Turn

Details...

Helix

7 – 12

6

Helix

14 – 30

17

Helix

37 – 65

29

Helix

68 – 74

7

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (B2/137) [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 440131FB758CC062	FASTA	137	15,033
	10 20 30 40 50 60 MTNMSCAYEL IKSLPAKLEQ LAQETQATIQ TLMIADPNVN KDLRAFCEFL TVQHQRAYRA 70 80 90 100 110 120 TNSLLIKPRV AAALRGEELD LGEADVAARV RQLKQQLAEL EMEIKPGHQQ VAQVSGRRKA 130 AAAAPVAQLG RVGVVNE
	Isoform 2 (B2/134) [UniParc]. Checksum: 8BFF5AC2293AA3BC	FASTA	134	14,687
	10 20 30 40 50 60 MSCAYELIKS LPAKLEQLAQ ETQATIQTLM IADPNVNKDL RAFCEFLTVQ HQRAYRATNS 70 80 90 100 110 120 LLIKPRVAAA LRGEELDLGE ADVAARVRQL KQQLAELEME IKPGHQQVAQ VSGRRKAAAA 130 APVAQLGRVG VVNE

References

[1]	"Recovery of infectivity from cDNA clones of nodamura virus and identification of small nonstructural proteins." Johnson K.L., Price B.D., Ball L.A. Virology 305:436-451(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], ALTERNATIVE INITIATION.
[2]	"Structure of the RNA-binding domain of nodamura virus protein B2, a suppressor of RNA interference." Korber S., Shaik Syed Ali P., Chen J.C. Biochemistry 48:2307-2309(2009) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 1-78, FUNCTION, RNA-BINDING.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF174533 Genomic RNA. Translation: AAF97861.1.

RefSeq

NP_077731.1. NC_002690.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3G80	X-ray	2.50	A/B	1-78	[»]

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

962118.

Family and domain databases

ProtoNet

Search...

Other

EvolutionaryTrace

Q9IMM3.

Entry information

Entry name

B2_NODAM

Accession

Primary (citable) accession number: Q9IMM3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 30, 2010
Last sequence update:	October 1, 2000
Last modified:	April 3, 2013
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program