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Q9IMM3

- B2_NODAM

UniProt

Q9IMM3 - B2_NODAM

Protein

Protein B2

Gene

B2

Organism
Nodamura virus (strain Mag115) (NoV)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Binds double-strand RNA (dsRNA) with high affinity. Suppresses the host RNA silencing-based antiviral response.1 Publication

    GO - Molecular functioni

    1. RNA binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    Suppressor of RNA silencing

    Keywords - Ligandi

    RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein B2
    Gene namesi
    Name:B2
    OrganismiNodamura virus (strain Mag115) (NoV)
    Taxonomic identifieri914672 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNodaviridaeAlphanodavirus
    Virus hostiAedes [TaxID: 7158]
    Lepidoptera (butterflies and moths) [TaxID: 7088]
    Sus scrofa (Pig) [TaxID: 9823]
    ProteomesiUP000008594: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 137137Protein B2PRO_0000402409Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    Secondary structure

    1
    137
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi7 – 126
    Helixi14 – 3017
    Helixi37 – 6529
    Helixi68 – 747

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3G80X-ray2.50A/B1-78[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9IMM3.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 7979RNA-bindingAdd
    BLAST

    Domaini

    The dsRNA-binding region is required for suppression of RNA silencing.By similarity

    Sequence similaritiesi

    Belongs to the nodaviridae B2 protein family.Curated

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative initiation. Align

    Isoform 1 (identifier: Q9IMM3-1) [UniParc]FASTAAdd to Basket

    Also known as: B2/137

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTNMSCAYEL IKSLPAKLEQ LAQETQATIQ TLMIADPNVN KDLRAFCEFL    50
    TVQHQRAYRA TNSLLIKPRV AAALRGEELD LGEADVAARV RQLKQQLAEL 100
    EMEIKPGHQQ VAQVSGRRKA AAAAPVAQLG RVGVVNE 137
    Length:137
    Mass (Da):15,033
    Last modified:October 1, 2000 - v1
    Checksum:i440131FB758CC062
    GO
    Isoform 2 (identifier: Q9IMM3-2) [UniParc]FASTAAdd to Basket

    Also known as: B2/134

    The sequence of this isoform differs from the canonical sequence as follows:
         1-3: Missing.

    Note: Produced by alternative initiation at Met-4 of isoform 1.

    Show »
    Length:134
    Mass (Da):14,687
    Checksum:i8BFF5AC2293AA3BC
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 33Missing in isoform 2. CuratedVSP_040284

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF174533 Genomic RNA. Translation: AAF97861.1.
    RefSeqiNP_077731.1. NC_002690.1.

    Genome annotation databases

    GeneIDi962118.

    Keywords - Coding sequence diversityi

    Alternative initiation

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF174533 Genomic RNA. Translation: AAF97861.1 .
    RefSeqi NP_077731.1. NC_002690.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3G80 X-ray 2.50 A/B 1-78 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 962118.

    Miscellaneous databases

    EvolutionaryTracei Q9IMM3.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Recovery of infectivity from cDNA clones of nodamura virus and identification of small nonstructural proteins."
      Johnson K.L., Price B.D., Ball L.A.
      Virology 305:436-451(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], ALTERNATIVE INITIATION.
    2. "Structure of the RNA-binding domain of nodamura virus protein B2, a suppressor of RNA interference."
      Korber S., Shaik Syed Ali P., Chen J.C.
      Biochemistry 48:2307-2309(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 1-78, FUNCTION, RNA-BINDING.

    Entry informationi

    Entry nameiB2_NODAM
    AccessioniPrimary (citable) accession number: Q9IMM3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 30, 2010
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 28 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Encoded on a subgenomic RNA (RNA3) synthesized during replication and which is co-terminal with RNA1.

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3