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Protein

Protein B2

Gene

B2

Organism
Nodamura virus (strain Mag115) (NoV)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Binds double-strand RNA (dsRNA) with high affinity. Suppresses the host RNA silencing-based antiviral response.1 Publication

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Suppressor of RNA silencing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein B2
Gene namesi
Name:B2
OrganismiNodamura virus (strain Mag115) (NoV)
Taxonomic identifieri914672 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNodaviridaeAlphanodavirus
Virus hostiAedes [TaxID: 7158]
Lepidoptera (butterflies and moths) [TaxID: 7088]
Sus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000008594 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 137137Protein B2PRO_0000402409Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

Secondary structure

1
137
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 126Combined sources
Helixi14 – 3017Combined sources
Helixi37 – 6529Combined sources
Helixi68 – 747Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3G80X-ray2.50A/B1-78[»]
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9IMM3.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 7979RNA-bindingAdd
BLAST

Domaini

The dsRNA-binding region is required for suppression of RNA silencing.By similarity

Sequence similaritiesi

Belongs to the nodaviridae B2 protein family.Curated

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform 1 (identifier: Q9IMM3-1) [UniParc]FASTAAdd to basket

Also known as: B2/137

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTNMSCAYEL IKSLPAKLEQ LAQETQATIQ TLMIADPNVN KDLRAFCEFL
60 70 80 90 100
TVQHQRAYRA TNSLLIKPRV AAALRGEELD LGEADVAARV RQLKQQLAEL
110 120 130
EMEIKPGHQQ VAQVSGRRKA AAAAPVAQLG RVGVVNE
Length:137
Mass (Da):15,033
Last modified:October 1, 2000 - v1
Checksum:i440131FB758CC062
GO
Isoform 2 (identifier: Q9IMM3-2) [UniParc]FASTAAdd to basket

Also known as: B2/134

The sequence of this isoform differs from the canonical sequence as follows:
     1-3: Missing.

Note: Produced by alternative initiation at Met-4 of isoform 1.

Show »
Length:134
Mass (Da):14,687
Checksum:i8BFF5AC2293AA3BC
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 33Missing in isoform 2. CuratedVSP_040284

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF174533 Genomic RNA. Translation: AAF97861.1.
RefSeqiNP_077731.1. NC_002690.1.

Genome annotation databases

GeneIDi962118.
KEGGivg:962118.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF174533 Genomic RNA. Translation: AAF97861.1.
RefSeqiNP_077731.1. NC_002690.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3G80X-ray2.50A/B1-78[»]
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi962118.
KEGGivg:962118.

Miscellaneous databases

EvolutionaryTraceiQ9IMM3.

Family and domain databases

ProtoNetiSearch...

Publicationsi

  1. "Recovery of infectivity from cDNA clones of nodamura virus and identification of small nonstructural proteins."
    Johnson K.L., Price B.D., Ball L.A.
    Virology 305:436-451(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], ALTERNATIVE INITIATION.
  2. "Structure of the RNA-binding domain of nodamura virus protein B2, a suppressor of RNA interference."
    Korber S., Shaik Syed Ali P., Chen J.C.
    Biochemistry 48:2307-2309(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 1-78, FUNCTION, RNA-BINDING.

Entry informationi

Entry nameiB2_NODAM
AccessioniPrimary (citable) accession number: Q9IMM3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: October 1, 2000
Last modified: April 1, 2015
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Encoded on a subgenomic RNA (RNA3) synthesized during replication and which is co-terminal with RNA1.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.