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Q9IMM3 (B2_NODAM) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 27. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein B2
Gene names
Name:B2
OrganismNodamura virus (strain Mag115) (NoV) [Reference proteome]
Taxonomic identifier914672 [NCBI]
Taxonomic lineageVirusesssRNA positive-strand viruses, no DNA stageNodaviridaeAlphanodavirus
Virus hostAedes [TaxID: 7158]
Lepidoptera (butterflies and moths) [TaxID: 7088]
Sus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length137 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds double-strand RNA (dsRNA) with high affinity. Suppresses the host RNA silencing-based antiviral response. Ref.2

Subunit structure

Homodimer By similarity.

Domain

The dsRNA-binding region is required for suppression of RNA silencing By similarity.

Miscellaneous

Encoded on a subgenomic RNA (RNA3) synthesized during replication and which is co-terminal with RNA1.

Sequence similarities

Belongs to the nodaviridae B2 protein family.

Ontologies

Keywords
   Coding sequence diversityAlternative initiation
   LigandRNA-binding
   Molecular functionSuppressor of RNA silencing
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Molecular_functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]
Isoform 1 (identifier: Q9IMM3-1)

Also known as: B2/137;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9IMM3-2)

Also known as: B2/134;

The sequence of this isoform differs from the canonical sequence as follows:
     1-3: Missing.
Note: Produced by alternative initiation at Met-4 of isoform 1.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 137137Protein B2
PRO_0000402409

Regions

Region1 – 7979RNA-binding

Natural variations

Alternative sequence1 – 33Missing in isoform 2.
VSP_040284

Secondary structure

......... 137
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (B2/137) [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 440131FB758CC062

FASTA13715,033
        10         20         30         40         50         60 
MTNMSCAYEL IKSLPAKLEQ LAQETQATIQ TLMIADPNVN KDLRAFCEFL TVQHQRAYRA 

        70         80         90        100        110        120 
TNSLLIKPRV AAALRGEELD LGEADVAARV RQLKQQLAEL EMEIKPGHQQ VAQVSGRRKA 

       130 
AAAAPVAQLG RVGVVNE 

« Hide

Isoform 2 (B2/134) [UniParc].

Checksum: 8BFF5AC2293AA3BC
Show »

FASTA13414,687

References

[1]"Recovery of infectivity from cDNA clones of nodamura virus and identification of small nonstructural proteins."
Johnson K.L., Price B.D., Ball L.A.
Virology 305:436-451(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], ALTERNATIVE INITIATION.
[2]"Structure of the RNA-binding domain of nodamura virus protein B2, a suppressor of RNA interference."
Korber S., Shaik Syed Ali P., Chen J.C.
Biochemistry 48:2307-2309(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 1-78, FUNCTION, RNA-BINDING.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF174533 Genomic RNA. Translation: AAF97861.1.
RefSeqNP_077731.1. NC_002690.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3G80X-ray2.50A/B1-78[»]
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID962118.

Family and domain databases

ProtoNetSearch...

Other

EvolutionaryTraceQ9IMM3.

Entry information

Entry nameB2_NODAM
AccessionPrimary (citable) accession number: Q9IMM3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: October 1, 2000
Last modified: April 3, 2013
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references