Reviewed, UniProtKB/Swiss-Prot Q9ILI5 (NS1AB_TASV2)

Names and origin

Protein names

Recommended name:
    Non-structural polyprotein 1AB
Cleaved into the following 5 chains:
    1- Recommended name:
            Protein p19
    2- Recommended name:
            Transmembrane protein 1A
    3- Recommended name:
            Serine protease p27
                Short name=p27
              EC=3.4.21.-
    4- Recommended name:
            Protein p20
    5- Recommended name:
            RNA-directed RNA polymerase p57
                Short name=p57
              EC=2.7.7.48

Gene names

Name:

ORF1

Organism

Turkey astrovirus 2 (TAstV-2)

Taxonomic identifier

246343 [NCBI]

Taxonomic lineage

Viruses › ssRNA positive-strand viruses, no DNA stage › Astroviridae › Avastrovirus

Virus host

Meleagris gallopavo (Common turkey) [TaxID: 9103]

Protein attributes

Sequence length	1638 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Non-structural polyprotein 1AB encodes for protease and genome replication/transcription proteins By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subcellular location	Host membrane; Multi-pass membrane protein Potential.
Post-translational modification	Cleaved presumably by viral and host proteases By similarity.
Sequence similarities	Belongs to the astroviridae polyprotein 1AB family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication
Cellular component	Membrane
Coding sequence diversity	Ribosomal frameshifting
Domain	Coiled coil Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Nucleotidyltransferase Protease RNA-directed RNA polymerase Thiol protease Transferase
Gene Ontology (GO)
Biological process	transcription, RNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW viral genome replication Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro RNA helicase activity Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by ribosomal frameshifting. [Align] [Select]

Isoform nsp1ab (identifier: Q9ILI5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Generated by a ribosomal frameshift at position 1125.

Isoform nsp1a (identifier: Q9ILI6-1)

The sequence of this isoform can be found in the external entry Q9ILI6-1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1638	1638	Non-structural polyprotein 1AB		PRO_0000327313
	Chain	1 – 170	170	Protein p19 Potential		PRO_0000327314
	Chain	171 – 552	382	Transmembrane protein 1A Potential		PRO_0000327315
	Chain	553 – 797	245	Serine protease p27 Potential		PRO_0000327316
	Chain	798 – 1051	254	Protein p20 Potential		PRO_0000327317
	Chain	1052 – 1638	587	RNA-directed RNA polymerase p57 Potential		PRO_0000327318
Regions
	Transmembrane	220 – 240	21	Potential
	Transmembrane	379 – 398	20	Potential
	Transmembrane	407 – 427	21	Potential
	Transmembrane	437 – 457	21	Potential
	Transmembrane	479 – 499	21	Potential
	Transmembrane	507 – 527	21	Potential
	Domain	1381 – 1515	135	RdRp catalytic
	Coiled coil	130 – 222	93	Potential
	Coiled coil	758 – 788	31	Potential
Sites
	Active site	600	1	Charge relay system; for serine protease activity By similarity
	Active site	632	1	Charge relay system; for serine protease activity By similarity
	Active site	697	1	Charge relay system; for serine protease activity By similarity
	Site	170 – 171	2	Cleavage Potential
	Site	552 – 553	2	Cleavage Potential
	Site	797 – 798	2	Cleavage Potential
	Site	1051 – 1052	2	Cleavage Potential

Sequences

	Sequence		Length	Mass (Da)
	Isoform nsp1ab [UniParc]. Last modified April 8, 2008. Version 2. Checksum: 941F4907479B6DB5	FASTA	1,638	187,664
	10 20 30 40 50 60 MAQAGRSGDA FASLDQRRER QEEQAQSGLD KVFYFQGVVE LFNRMKIAYG RTPAWTALMK 70 80 90 100 110 120 CNAIYLKDFK TAVGVEGTRY GLFFAEEVTK PTWSPDIGAN LITLGEKACL DAQNAKYERL 130 140 150 160 170 180 QASLKTTSGL VHQVMEKTRE AKENLEKANK IQEQLDKVIE SNKALHRKIQ ERNREKMQEY 190 200 210 220 230 240 MVRLHNTQKD RDDWVQRCSR LEQENVTLQK RLKEKENALV SVGWDLLGWI VISVLVFGLI 250 260 270 280 290 300 SLADAQNLTP PAKIVITPGQ AEFMDLAKLE KIQVRKYRLD SCELPPEKGC VLYKDYLTTR 310 320 330 340 350 360 PVSFLELMAK CSKPDWVSES SYNETTLMEE CIQIFGAEWC EGKLVDLVPR KCGEQHVLVN 370 380 390 400 410 420 IIEQIEKTRE VVTLIYGKVM SYRLDMWITS IFSLVLAGNK EKLFKMAPFI FVAWFLNIPV 430 440 450 460 470 480 FLTCVAVNIF PVVSLPFILF QIFMPQFVLV NAFLLWLTLT LTAFYWSEGP KILMEISYAL 490 500 510 520 530 540 VYTIGFVLWS LGLAVGVTLK LTMVHQILMF CVVAAAICGT KFACTTITVQ HPDGTTAKYT 550 560 570 580 590 600 RVGKLKNNVV NQCKKVVTTL QTRGVIPATP AKTASIVIVE GKNGTGVGFR FMNYILTAEH 610 620 630 640 650 660 VVQGSDIATL KNGSVSVKSK VIKTIPIFES VDNVAVLKLP PELNSVKPIK LAKKVQSDYL 670 680 690 700 710 720 TLTAYDPNFQ HAATFTGWCI IDGNWLNNSF DTKFGNSGAP YCDHDGRLVG IHLGTQGVLS 730 740 750 760 770 780 QGIVIVDALK NTFQLADQCR PQNFDMDEFL EKVIAGTKVS HAAILKELEE LREEVQFLKK 790 800 810 820 830 840 KCVTYDDYWL CQTIFGQAKG KTKKTVRGRK HLVTKRALGK GHFMKMRMLT DEEYQNMIEK 850 860 870 880 890 900 GFSAEEIREA VNALREQAWL NYCIDNDVDD EGEEDWYDDM VETDRVNQEI DEAIERAMED 910 920 930 940 950 960 RGEFYQKKSR LTFVEQAMMH LIQVSKERSQ TAKLEVQKEN EAQLVKMFER CVTDENTPEG 970 980 990 1000 1010 1020 TTSIAALSTE DDVRLVEGKV IDFTKAKNIP VDGEIRREII PGTKCTEIST GPENKKNILK 1030 1040 1050 1060 1070 1080 KKDTHIAEGK VETKSSQQPV DVKDDKPVAL EQRKPRACKW CGSSQKHDYR ECRFQREKRF 1090 1100 1110 1120 1130 1140 CVYCAAMHSM FEGHIRPIEC TSCKKSFSGI EKLEDHVVSG ECQKKLIEGP VTTKAPTPVP 1150 1160 1170 1180 1190 1200 DWLKIFAWED DILPPEGKTA LPENVTLIGH IPVDKLVSRT KKVQDPLLGL VTPWKQDMYD 1210 1220 1230 1240 1250 1260 STTWTVKAYT KMFEKFHYHD PVDFVEQYAE FVLLCDNMVL REHDYMANSN ITPIMSTEKN 1270 1280 1290 1300 1310 1320 VNSTPAYPKF QAYDSEAEYL EDCGWQEYLD VVSDPETINR RPLWWCFLKN EVLKREKIED 1330 1340 1350 1360 1370 1380 SDIRMILCTD PIFTRIGAMF EQDQNNRMKQ QTEIRSAQVG WTPFFGGLDR RVRRLYGDGD 1390 1400 1410 1420 1430 1440 RYFVEMDWTR YDGTIPKSLF WRIRQIRFFF LHDSHKTPKM RRLYNWYVKN LLEKIILLPT 1450 1460 1470 1480 1490 1500 GEVCQVKKGN PSGQFSTTVD NNMINVWLTT FEVSYLFFKQ RGRLPTEKEL QENCSMICYG 1510 1520 1530 1540 1550 1560 DDRLLSIRKG FVEYEPDTVI DMYKNIFGMW VKRNNIKIQD TPEGLSFCGL TIVKSSTGAY 1570 1580 1590 1600 1610 1620 VGVPNVNKIL STLENPVRRL PDVESLWGKL VSLRILCENA PSNVKHFLDE QISNVEEFAA 1630 RENIQLPEVG PDFYSRIW
	Isoform nsp1a. See Q9ILI6.	FASTA	–	–

References

[1]	"Molecular characterization of an avian astrovirus." Koci M.D., Seal B.S., Schultz-Cherry S. J. Virol. 74:6173-6177(2000) [PubMed: 10846102] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF206663 Genomic RNA. Translation: AAF60952.1. Sequence problems.
RefSeq	NP_987087.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2943319.
Enzyme and pathway databases
BRENDA	2.7.7.48. 314525.
Family and domain databases
InterPro	IPR001850. Peptidase_S7. IPR001205. RNA_pol_P3D. IPR007094. RNA_pol_PSvir. [Graphical view]
Pfam	PF00949. Peptidase_S7. 1 hit. PF00680. RdRP_1. 1 hit. [Graphical view]
PROSITE	PS50507. RDRP_SSRNA_POS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NS1AB_TASV2

Accession

Primary (citable) accession number: Q9ILI5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	April 8, 2008
Last modified:	June 16, 2009
This is version 37 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)