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Protein

Hemagglutinin-esterase

Gene

HE

Organism
Rat coronavirus (strain 681) (RCV-SDAV) (Sialodacryoadenitis virus SDAV-681)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-4-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. May become a target for both the humoral and the cellular branches of the immune system.UniRule annotation

Catalytic activityi

N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei45NucleophileUniRule annotation1
Active sitei342Charge relay systemUniRule annotation1
Active sitei345Charge relay systemUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHemagglutinin, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-esteraseUniRule annotation (EC:3.1.1.53UniRule annotation)
Short name:
HE proteinUniRule annotation
Alternative name(s):
E3 glycoproteinUniRule annotation
Gene namesi
Name:HEUniRule annotation
OrganismiRat coronavirus (strain 681) (RCV-SDAV) (Sialodacryoadenitis virus SDAV-681)
Taxonomic identifieri33740 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiRattus norvegicus (Rat) [TaxID: 10116]

Subcellular locationi

  • Virion membrane UniRule annotation; Single-pass type I membrane protein UniRule annotation
  • Host cell membrane UniRule annotation; Single-pass type I membrane protein UniRule annotation
  • Note: In infected cells becomes incorporated into the envelope of virions during virus assembly at the endoplasmic reticulum and cis Golgi. However, some may escape incorporation into virions and subsequently migrate to the cell surface.UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 407Virion surfaceUniRule annotationAdd BLAST385
Transmembranei408 – 428HelicalUniRule annotationAdd BLAST21
Topological domaini429 – 439IntravirionUniRule annotationAdd BLAST11

GO - Cellular componenti

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22UniRule annotationAdd BLAST22
ChainiPRO_000003715223 – 439Hemagglutinin-esteraseUniRule annotationAdd BLAST417

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi49 ↔ 70UniRule annotation
Glycosylationi94N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Disulfide bondi118 ↔ 167UniRule annotation
Glycosylationi196N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Disulfide bondi202 ↔ 291UniRule annotation
Disulfide bondi210 ↔ 264UniRule annotation
Glycosylationi246N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Glycosylationi309N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Glycosylationi316N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Disulfide bondi322 ↔ 327UniRule annotation
Glycosylationi331N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Glycosylationi360N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Disulfide bondi363 ↔ 387UniRule annotation
Glycosylationi374N-linked (GlcNAc...) asparagine; by hostUniRule annotation1

Post-translational modificationi

N-glycosylated in the host RER.UniRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Forms a complex with the M protein in the pre-Golgi. Associates then with S-M complex to form a ternary complex S-M-HE.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ9IKD2
SMRiQ9IKD2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni12 – 132Esterase domain 1UniRule annotationAdd BLAST121
Regioni133 – 281Receptor bindingUniRule annotationAdd BLAST149
Regioni282 – 395Esterase domain 2UniRule annotationAdd BLAST114

Sequence similaritiesi

Belongs to the influenza type C/coronaviruses hemagglutinin-esterase family.UniRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

HAMAPiMF_04207 BETA_CORONA_HE, 1 hit
InterProiView protein in InterPro
IPR008980 Capsid_hemagglutn
IPR007142 Hemagglutn-estrase_core
IPR003860 Hemagglutn-estrase_hemagglutn
PfamiView protein in Pfam
PF03996 Hema_esterase, 1 hit
PF02710 Hema_HEFG, 1 hit
SUPFAMiSSF49818 SSF49818, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9IKD2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRMCIAMAP RTLLLLIGCQ LVFGFNEPIN IVSHLNDDWF LFGDSRSDCT
60 70 80 90 100
YVENNGHPKL DWLDLDPKLC NSGKIAAKSG NSLFRSFHFT DFYNYTGEGD
110 120 130 140 150
QIIFYEGVSF SPSHGFKCLV EGDNNKWMGN KARFYALLYK KMAQYRSLSF
160 170 180 190 200
VTVPYAYGGN AKPTSICKDK TLTLNNPTFI SKESNYVDYY YVSEANFTLQ
210 220 230 240 250
GCDEFIVPLC VFNGHSRGSS SDPANKYYTD SQSYYNIDTG VLYGFNSTLD
260 270 280 290 300
VGNTAQNPGL DLTCMYLVLT PGNYKAVSLE YLLTIPSKAI CLRKPKRFMP
310 320 330 340 350
VQVVDSRWNS TRQSDNMTAV ACQLPYCFFR NTSADYSGDT HDVHHGDFYF
360 370 380 390 400
RQLLSGLLYN VSCIAQQGAF LYNNVSSIWP VYGYGHCPTA ANIGYMAPVC
410 420 430
LYDPLPVILL GVLLGIAVLI IVFLILYFMA DSSVRLHEA
Length:439
Mass (Da):49,260
Last modified:October 1, 2000 - v1
Checksum:i184E3AECFCE4EF53
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF207551 Genomic RNA Translation: AAF97737.1

Similar proteinsi

Entry informationi

Entry nameiHEMA_CVRSD
AccessioniPrimary (citable) accession number: Q9IKD2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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