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Q9IKD2

- HEMA_CVRSD

UniProt

Q9IKD2 - HEMA_CVRSD

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Protein

Hemagglutinin-esterase

Gene

HE

Organism
Rat coronavirus (strain 681) (RCV-SDAV) (Sialodacryoadenitis virus SDAV-681)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-4-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system By similarity.By similarity

Catalytic activityi

N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei45 – 451NucleophileBy similarity
Active sitei342 – 3421Charge relay systemBy similarity
Active sitei345 – 3451Charge relay systemBy similarity

GO - Molecular functioni

  1. sialate O-acetylesterase activity Source: UniProtKB-EC

GO - Biological processi

  1. fusion of virus membrane with host plasma membrane Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-esterase (EC:3.1.1.53)
Short name:
HE protein
Alternative name(s):
E3 glycoprotein
Gene namesi
Name:HE
OrganismiRat coronavirus (strain 681) (RCV-SDAV) (Sialodacryoadenitis virus SDAV-681)
Taxonomic identifieri33740 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiRattus norvegicus (Rat) [TaxID: 10116]

Subcellular locationi

Virion membrane Curated; Single-pass type I membrane protein Curated. Host cell membrane Curated; Single-pass type I membrane protein Curated
Note: In infected cells becomes incorporated into the envelope of virions during virus assembly at the endoplasmic reticulum and cis Golgi. However, some may escape incorporation into virions and subsequently migrate to the cell surface By similarity.By similarity

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
  3. viral envelope Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Chaini25 – 439415Hemagglutinin-esterasePRO_0000037152Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi49 ↔ 70By similarity
Glycosylationi94 – 941N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi118 ↔ 167By similarity
Glycosylationi196 – 1961N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi202 ↔ 291By similarity
Disulfide bondi210 ↔ 264By similarity
Glycosylationi246 – 2461N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi309 – 3091N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi316 – 3161N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi322 ↔ 327By similarity
Glycosylationi331 – 3311N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi360 – 3601N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi363 ↔ 387By similarity
Glycosylationi374 – 3741N-linked (GlcNAc...); by hostSequence Analysis

Post-translational modificationi

N-glycosylated in the RER.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Forms a complex with the M protein in the pre-Golgi. Associates then with S-M complex to form a ternary complex S-M-HE.

Structurei

3D structure databases

ProteinModelPortaliQ9IKD2.
SMRiQ9IKD2. Positions 26-392.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini25 – 407383Virion surfaceSequence AnalysisAdd
BLAST
Topological domaini429 – 43911IntravirionSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei408 – 42821HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni12 – 132121Esterase domain first partBy similarityAdd
BLAST
Regioni133 – 281149Receptor bindingBy similarityAdd
BLAST
Regioni282 – 395114Esterase domain second partBy similarityAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
[Graphical view]
PfamiPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view]
SUPFAMiSSF49818. SSF49818. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9IKD2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGRMCIAMAP RTLLLLIGCQ LVFGFNEPIN IVSHLNDDWF LFGDSRSDCT
60 70 80 90 100
YVENNGHPKL DWLDLDPKLC NSGKIAAKSG NSLFRSFHFT DFYNYTGEGD
110 120 130 140 150
QIIFYEGVSF SPSHGFKCLV EGDNNKWMGN KARFYALLYK KMAQYRSLSF
160 170 180 190 200
VTVPYAYGGN AKPTSICKDK TLTLNNPTFI SKESNYVDYY YVSEANFTLQ
210 220 230 240 250
GCDEFIVPLC VFNGHSRGSS SDPANKYYTD SQSYYNIDTG VLYGFNSTLD
260 270 280 290 300
VGNTAQNPGL DLTCMYLVLT PGNYKAVSLE YLLTIPSKAI CLRKPKRFMP
310 320 330 340 350
VQVVDSRWNS TRQSDNMTAV ACQLPYCFFR NTSADYSGDT HDVHHGDFYF
360 370 380 390 400
RQLLSGLLYN VSCIAQQGAF LYNNVSSIWP VYGYGHCPTA ANIGYMAPVC
410 420 430
LYDPLPVILL GVLLGIAVLI IVFLILYFMA DSSVRLHEA
Length:439
Mass (Da):49,260
Last modified:October 1, 2000 - v1
Checksum:i184E3AECFCE4EF53
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF207551 Genomic RNA. Translation: AAF97737.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF207551 Genomic RNA. Translation: AAF97737.1 .

3D structure databases

ProteinModelPortali Q9IKD2.
SMRi Q9IKD2. Positions 26-392.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
[Graphical view ]
Pfami PF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view ]
SUPFAMi SSF49818. SSF49818. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Primary structure of the sialodacryoadenitis virus genome: sequence of the structural-protein region and its application for differential diagnosis."
    Yoo D., Pei Y., Christie N., Cooper M.
    Clin. Diagn. Lab. Immunol. 7:568-573(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiHEMA_CVRSD
AccessioniPrimary (citable) accession number: Q9IKD2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: October 1, 2000
Last modified: October 29, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3