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Q9IKD2

- HEMA_CVRSD

UniProt

Q9IKD2 - HEMA_CVRSD

Protein

Hemagglutinin-esterase

Gene

HE

Organism
Rat coronavirus (strain 681) (RCV-SDAV) (Sialodacryoadenitis virus SDAV-681)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-4-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system By similarity.By similarity

    Catalytic activityi

    N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei45 – 451NucleophileBy similarity
    Active sitei342 – 3421Charge relay systemBy similarity
    Active sitei345 – 3451Charge relay systemBy similarity

    GO - Molecular functioni

    1. sialate O-acetylesterase activity Source: UniProtKB-EC

    GO - Biological processi

    1. fusion of virus membrane with host plasma membrane Source: InterPro

    Keywords - Molecular functioni

    Hemagglutinin, Hydrolase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hemagglutinin-esterase (EC:3.1.1.53)
    Short name:
    HE protein
    Alternative name(s):
    E3 glycoprotein
    Gene namesi
    Name:HE
    OrganismiRat coronavirus (strain 681) (RCV-SDAV) (Sialodacryoadenitis virus SDAV-681)
    Taxonomic identifieri33740 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
    Virus hostiRattus norvegicus (Rat) [TaxID: 10116]

    Subcellular locationi

    Virion membrane Curated; Single-pass type I membrane protein Curated. Host cell membrane Curated; Single-pass type I membrane protein Curated
    Note: In infected cells becomes incorporated into the envelope of virions during virus assembly at the endoplasmic reticulum and cis Golgi. However, some may escape incorporation into virions and subsequently migrate to the cell surface By similarity.By similarity

    GO - Cellular componenti

    1. host cell plasma membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. viral envelope Source: UniProtKB-KW
    4. virion membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Chaini25 – 439415Hemagglutinin-esterasePRO_0000037152Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi49 ↔ 70By similarity
    Glycosylationi94 – 941N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi118 ↔ 167By similarity
    Glycosylationi196 – 1961N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi202 ↔ 291By similarity
    Disulfide bondi210 ↔ 264By similarity
    Glycosylationi246 – 2461N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi309 – 3091N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi316 – 3161N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi322 ↔ 327By similarity
    Glycosylationi331 – 3311N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi360 – 3601N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi363 ↔ 387By similarity
    Glycosylationi374 – 3741N-linked (GlcNAc...); by hostSequence Analysis

    Post-translational modificationi

    N-glycosylated in the RER.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked. Forms a complex with the M protein in the pre-Golgi. Associates then with S-M complex to form a ternary complex S-M-HE.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9IKD2.
    SMRiQ9IKD2. Positions 26-392.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini25 – 407383Virion surfaceSequence AnalysisAdd
    BLAST
    Topological domaini429 – 43911IntravirionSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei408 – 42821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni12 – 132121Esterase domain first partBy similarityAdd
    BLAST
    Regioni133 – 281149Receptor bindingBy similarityAdd
    BLAST
    Regioni282 – 395114Esterase domain second partBy similarityAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Family and domain databases

    InterProiIPR008980. Capsid_hemagglutn.
    IPR007142. Hemagglutn-estrase_core.
    IPR003860. Hemagglutn-estrase_hemagglutn.
    [Graphical view]
    PfamiPF03996. Hema_esterase. 1 hit.
    PF02710. Hema_HEFG. 1 hit.
    [Graphical view]
    SUPFAMiSSF49818. SSF49818. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9IKD2-1 [UniParc]FASTAAdd to Basket

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    MGRMCIAMAP RTLLLLIGCQ LVFGFNEPIN IVSHLNDDWF LFGDSRSDCT    50
    YVENNGHPKL DWLDLDPKLC NSGKIAAKSG NSLFRSFHFT DFYNYTGEGD 100
    QIIFYEGVSF SPSHGFKCLV EGDNNKWMGN KARFYALLYK KMAQYRSLSF 150
    VTVPYAYGGN AKPTSICKDK TLTLNNPTFI SKESNYVDYY YVSEANFTLQ 200
    GCDEFIVPLC VFNGHSRGSS SDPANKYYTD SQSYYNIDTG VLYGFNSTLD 250
    VGNTAQNPGL DLTCMYLVLT PGNYKAVSLE YLLTIPSKAI CLRKPKRFMP 300
    VQVVDSRWNS TRQSDNMTAV ACQLPYCFFR NTSADYSGDT HDVHHGDFYF 350
    RQLLSGLLYN VSCIAQQGAF LYNNVSSIWP VYGYGHCPTA ANIGYMAPVC 400
    LYDPLPVILL GVLLGIAVLI IVFLILYFMA DSSVRLHEA 439
    Length:439
    Mass (Da):49,260
    Last modified:October 1, 2000 - v1
    Checksum:i184E3AECFCE4EF53
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF207551 Genomic RNA. Translation: AAF97737.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF207551 Genomic RNA. Translation: AAF97737.1 .

    3D structure databases

    ProteinModelPortali Q9IKD2.
    SMRi Q9IKD2. Positions 26-392.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR008980. Capsid_hemagglutn.
    IPR007142. Hemagglutn-estrase_core.
    IPR003860. Hemagglutn-estrase_hemagglutn.
    [Graphical view ]
    Pfami PF03996. Hema_esterase. 1 hit.
    PF02710. Hema_HEFG. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49818. SSF49818. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure of the sialodacryoadenitis virus genome: sequence of the structural-protein region and its application for differential diagnosis."
      Yoo D., Pei Y., Christie N., Cooper M.
      Clin. Diagn. Lab. Immunol. 7:568-573(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiHEMA_CVRSD
    AccessioniPrimary (citable) accession number: Q9IKD2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 16, 2003
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3