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Protein

Envelopment polyprotein

Gene

GP

Organism
Tomato spotted wilt virus (isolate D) (TSWV)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Glycoprotein N: Together with Glycoprotein C are present at the surface of the virion. They are able to attach the virion to a cell receptor and to promote fusion of membranes after endocytosis of the virion (By similarity).By similarity
Glycoprotein C: Together with Glycoprotein N are present at the surface of the virion. They are able to attach the virion to a cell receptor and to promote fusion of membranes after endocytosis of the virion (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Fusion of virus membrane with host endosomal membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Envelopment polyprotein
Alternative name(s):
M polyprotein
Cleaved into the following 2 chains:
Glycoprotein NBy similarity
Short name:
Gn
Alternative name(s):
Glycoprotein G1
Glycoprotein CBy similarity
Short name:
Gc
Alternative name(s):
Glycoprotein G2
Gene namesi
Name:GP
OrganismiTomato spotted wilt virus (isolate D) (TSWV)
Taxonomic identifieri267288 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesBunyaviridaeTospovirus
Virus hostiFrankliniella occidentalis (Western flower thrips) [TaxID: 133901]
Scirtothrips dorsalis [TaxID: 163899]
Solanum lycopersicum (Tomato) (Lycopersicon esculentum) [TaxID: 4081]
Thrips tabaci [TaxID: 161014]

Subcellular locationi

Glycoprotein N :
  • Virion membrane By similarity; Single-pass type I membrane protein By similarity
  • Host Golgi apparatus membrane By similarity; Single-pass type I membrane protein By similarity
  • Host endoplasmic reticulum membrane By similarity; Single-pass type I membrane protein By similarity

  • Note: Glycoprotein C alone is retained in the membrane of the endoplasmic reticulum, but not transported to the Golgi. Coexpression of Glycoprotein C and Glycoprotein N results in efficient transport of Glycoprotein C to the Golgi complex, indicating that their interaction is essential for proper targeting to this organelle, where virion budding occurs.By similarity
Glycoprotein C :
  • Virion membrane By similarity; Single-pass type I membrane protein By similarity
  • Host Golgi apparatus membrane By similarity; Single-pass type I membrane protein By similarity

  • Note: Glycoprotein G2 is retained in the Golgi complex and probably contains a Golgi retention signal.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini36 – 345LumenalSequence analysisAdd BLAST310
Transmembranei346 – 366HelicalSequence analysisAdd BLAST21
Topological domaini367 – 484CytoplasmicSequence analysisAdd BLAST118
Topological domaini485 – 1067LumenalSequence analysisAdd BLAST583
Transmembranei1068 – 1088HelicalSequence analysisAdd BLAST21
Topological domaini1089 – 1135CytoplasmicSequence analysisAdd BLAST47

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host endoplasmic reticulum, Host Golgi apparatus, Host membrane, Membrane, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 35Sequence analysisAdd BLAST35
ChainiPRO_000003686236 – 1135Envelopment polyproteinAdd BLAST1100
ChainiPRO_000003686336 – 484Glycoprotein NBy similarityAdd BLAST449
ChainiPRO_0000036864485 – 1135Glycoprotein CBy similarityAdd BLAST651

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi116N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi210N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi340N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi588N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi605N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi980N-linked (GlcNAc...); by hostSequence analysis1

Post-translational modificationi

Specific enzymatic cleavages in vivo yield mature proteins including glycoprotein G1 and glycoprotein G2.By similarity
Glycosylated. Glycosylation is essential for proper subcellular location (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei484 – 485Cleavage; by host signal peptidaseBy similarity2

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ9IKB7.

Interactioni

Subunit structurei

Glycoprotein N: Interacts with Glycoprotein C and nucleoprotein. Glycoprotein C: Interacts with Glycoprotein N and nucleoprotein.By similarity

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi41 – 43Cell attachment siteSequence analysis3

Domaini

The cell attachment site present in these glycoproteins may help in the adhesion of virus to cells.

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR005167. Bunya_G1.
IPR014414. M_poly_TospoV.
[Graphical view]
PfamiPF03557. Bunya_G1. 1 hit.
[Graphical view]
PIRSFiPIRSF003960. M_poly_TospoV. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9IKB7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRILKLLELV VKVSLFTIAL SSVLLAFLTF RATDAKVEII RGDHPEIYDD
60 70 80 90 100
SAENEVPTAA SIQREAILET LTNLVLESRT PGTRQIREEK LTIPISTEPA
110 120 130 140 150
TQKTISVLDL PNNCLNASSL KCEIKGISTY NVYYQVENNG VIYSSVSDSA
160 170 180 190 200
EGLEKCDNSL NLPKRFSKVP VIPITKLDNK RHFSVGTNFF IPESLTQDNY
210 220 230 240 250
PITYNSYPTN GTVSLQTVKL SGDCKITKSN FANPYTVSIT SPEKIMGYLI
260 270 280 290 300
KKPGENVEHK VIAFSGSASI TFTEEMLDGE HNLLCGDKSA KIPKTNKRVR
310 320 330 340 350
DCIIKYSKSI YKQTACINFS WIRLILIALL IYFPIRWLVN KTTKPLFLWY
360 370 380 390 400
DLMGLITYPV LLLINCLWKY FPFKCSNCGN LCIVTRECTK VCICNKSKAS
410 420 430 440 450
KEHSSECPIL SKEADHDYNK HKWTSMEWFH LIVNTKLSLS LLKFVTEILI
460 470 480 490 500
GLVILSQIPM SMAQTTQCLS GCFYVPGCPF LVTSKFEKCP EKDQCYCNVK
510 520 530 540 550
EDKIIESIFG TNIVIEGPND CIENQNCIAR PSIDNLIKCR LGCEYLDLFQ
560 570 580 590 600
NKPLYNGFSD YTESSLGLTS VGLYEAKRLR NGIIDSYNRT DKISGMIAGD
610 620 630 640 650
SLDKNETSIP ENILPRQSLI FDSVVDGKYR YMIEQSLLGG GGTIFMLNDK
660 670 680 690 700
TSETAKKFVI YIKSVGIHHE VSEEYTTAPI QSTHTDFYPT CIGNCDTCRK
710 720 730 740 750
NQALTGFQDF CITPTSYWGC EEAWCFAINE GATCGFCRNI YDIDKSYRIY
760 770 780 790 800
SVLKSTIVAD VCISGILGGQ CSRITEEVPY ENTLFQADIQ SDLHNDGITI
810 820 830 840 850
GELIAHGPDS HIYSGNIANL NDPVKMFGHP QLTHDGVPIF TKKTLEGDDM
860 870 880 890 900
SWDCAATGKK SVTIKTCGYD TYRFRSGLEQ ISDIPVSFKD FSSFFLEKSF
910 920 930 940 950
SLGELKIVVD LPSDLFKVVP KKPSITSTSL NCNGCLLCGQ GLSCILEFFS
960 970 980 990 1000
DLTFSTAISI DACSLSTYQL AVKKGSNKYN ITMFCSANPD KKKMTLYPEG
1010 1020 1030 1040 1050
NPDIPVEVLV NNVIIEEPEN IIDQNDEYAH EEQQYNSDSS AWGFWDYIKS
1060 1070 1080 1090 1100
PFNFIASYFG SFFDTIRVVL LIAFIFLVIY FCSILTSICK GYVKHKSYKS
1110 1120 1130
RSKIEDDDEP EIKAPMLMKD TMTRRRPPMD FSHLV
Length:1,135
Mass (Da):127,554
Last modified:October 1, 2000 - v1
Checksum:i53F271999CABEC33
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF208497 Genomic RNA. Translation: AAF80979.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF208497 Genomic RNA. Translation: AAF80979.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ9IKB7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR005167. Bunya_G1.
IPR014414. M_poly_TospoV.
[Graphical view]
PfamiPF03557. Bunya_G1. 1 hit.
[Graphical view]
PIRSFiPIRSF003960. M_poly_TospoV. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGP_TSWVD
AccessioniPrimary (citable) accession number: Q9IKB7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: October 1, 2000
Last modified: October 5, 2016
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.