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Q9IJX4 (POLN_CRPVC) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Protein attributes

Sequence length1771 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Protein 1A functions as a suppressor of RNA-mediated gene silencing, an antiviral defense mechanism of insect cells. Inhibits siRNA function, but does not interfere with miRNA pathway. Does not bind to dsRNA or siRNA. Ref.3

RNA-directed RNA polymerase replicates genomic and antigenomic RNA. Ref.3

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Subunit structure

Protein 1A interacts with host AGO2; this interaction may block the RNA-induced silencing complexes (RISC) activity. Ref.3

Post-translational modification

Protein 1A might be expressed through a ribosomal skip from one codon to the next without formation of a peptide bond.

Sequence similarities

Contains 1 RdRp catalytic domain.

Contains 1 SF3 helicase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 17711771Replicase polyprotein
PRO_0000398372
Chain1 – 190190Protein 2A Potential
PRO_0000398373
Chain1 – 166166Protein 1A Potential
PRO_0000398374
Chain191 – 328138Protein 2B Potential
PRO_0000398375
Chain329 – 739411Protein 2C Potential
PRO_0000398376
Chain740 – ?Protein 3A PotentialPRO_0000398377
Chain? – 908Protein 3B PotentialPRO_0000398378
Chain909 – 12203123C-like protease Potential
PRO_0000398379
Chain1221 – 1771551RNA-directed RNA polymerase 3D-POL Potential
PRO_0000398380

Regions

Domain482 – 656175SF3 helicase
Domain1495 – 1634140RdRp catalytic
Nucleotide binding510 – 5178ATP Potential
Coiled coil1 – 2929 Potential
Coiled coil1385 – 141329 Potential

Sites

Active site11621 Potential
Site166 – 1672Cleavage; by ribosomal skip Potential
Site190 – 1912Cleavage; by 3C-like protease Potential
Site328 – 3292Cleavage; by 3C-like protease Potential
Site739 – 7402Cleavage; by 3C-like protease Potential
Site884 – 8852Cleavage; by 3C-like protease Potential
Site908 – 9092Cleavage; by 3C-like protease Potential
Site1220 – 12212Cleavage; by 3C-like protease Potential

Sequences

Sequence LengthMass (Da)Tools
Q9IJX4 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 78C5969469EAA716

FASTA1,771203,830
        10         20         30         40         50         60 
MSFQQTNNNA TNNINSLEEL AAQELIAAQF EGNLDGFFCT FYVQSKPQLL DLESECYCMD 

        70         80         90        100        110        120 
DFDCGCDRIK REEELRKLIF LTSDVYGYNF EEWKGLVWKF VQNYCPEHRY GSTFGNGLLI 

       130        140        150        160        170        180 
VSPRFFMDHL DWFQQWKLVS SNDECRAFLR KRTQLLMSGD VESNPGPVQS RPVYACDNDP 

       190        200        210        220        230        240 
RAIRLEKALQ RRDEKISTLI KKLRQEIKNN RIYTQGFFDD LKGAKGEVGQ LNGNLTRICD 

       250        260        270        280        290        300 
FLENSLPTLT AQIQTTVLTT TDKYVNLKED LLKVAILLVL VRLLMVWKKY RAALIVIILF 

       310        320        330        340        350        360 
VMHFYGFDKQ ILDIVLDLKD KILQTTTQAG TETLEEVVYH PWFDTCGKLI FAVLAFFAIK 

       370        380        390        400        410        420 
KIPGKQDWDN YISRLDRIPK AIEGSKKIVD YCSEYFNLSV DEVKKVVLGK ELKGTQGLYD 

       430        440        450        460        470        480 
EIHVWAKEIR HYLDLDERNK ITLDTETAAK VEDLYKRGLK YSEEKIPDRD IARFITTMLF 

       490        500        510        520        530        540 
PAKSLYEQVL LSPVKGGGPK MRPITVWLTG ESGIGKTQMI YPLCIDILRE MGIVKPDAYK 

       550        560        570        580        590        600 
HQAYARQVET EYWDGYNGQK IVIYDDAFQL KDDKTKPNPE IFEVIRTCNT FPQHLHMAAL 

       610        620        630        640        650        660 
QDKNMYSQAE VLLYTTNQFQ VQLESITFPD AFYNRMKTHA YRVQIKQEKS IWVRNARGEE 

       670        680        690        700        710        720 
YNALDVTKLN KDEAIDLSVY EFQKMRFDDE SATKWIDDGE PISYDEFART ICKAWKEEKE 

       730        740        750        760        770        780 
KTFHQLQWLE AYASRTVAQG GSETSEYYDV WDETYFSNLL SQGFMAGKSL IEMEAEFASD 

       790        800        810        820        830        840 
AETFNAYIEY KKNIPKETKW SKWMTILDEQ ISALSTKIRE LKNKAYKFIS EHPYLTALGF 

       850        860        870        880        890        900 
IGVMISAFAM YSFFERTLTD DTITSEVGSS GDNKTQKISK RVVEVGGSGD VKTTKPAKTA 

       910        920        930        940        950        960 
VEVGSSGDSK TMKNKITKVE VGSSGDSKTQ KQRNTKVEVG KELEKEAETQ GCSDPAAHAL 

       970        980        990       1000       1010       1020 
VLDVLQKNTY CLYYERMVKG EMKRYRLATA TFLRGWVCMM PYHFIETLYA RKVAPSTNIY 

      1030       1040       1050       1060       1070       1080 
FSQPNCDDVI VVPVSHFIAP NAERVELTTA CTRIHYKDET PRDCVLVNLH RRMCHPHRDI 

      1090       1100       1110       1120       1130       1140 
LKHFVKKSDQ GNLRGVFQGT LATFHQSANE LCRAYQWLQA IRPLDQEITI YHEDTDMFDY 

      1150       1160       1170       1180       1190       1200 
ESESYTQRDC YEYNAPTQTG NCGSIVGLYN KRMERKLIGM HIPGNVSECH GYACPLTQEA 

      1210       1220       1230       1240       1250       1260 
IMDGLNRLEK LDPVNNITVQ CCFEPPSDIK DTMSGETPEG KFCAIGKSNI KVGQAVKTTL 

      1270       1280       1290       1300       1310       1320 
LKSCIYGMLS KPITKPAHLT RTRLPNGEIV DPLMKGLKKC GVDTAVLDAE IVESAALDVK 

      1330       1340       1350       1360       1370       1380 
QVVLTQYNSM LDVNKYRRFL TYEEATQGTG DDDFMKGIAR QTSPGYRYFQ MPRKLPGKQD 

      1390       1400       1410       1420       1430       1440 
WMGSGEQYDF TSQRAQELRR DVEELIDNCA KGIIKDVVFV DTLKDERRPI EKVDAGKTRV 

      1450       1460       1470       1480       1490       1500 
FSAGPQHFVV AFRKYFLPFA AYLMNNRIDN EIAVGTNVYS TDWERIAKRL KKHGNKVIAG 

      1510       1520       1530       1540       1550       1560 
DFGNFDGSLV AQFFGQSCGK SFYPWFKTFN DVNTEDGKRN LMICIGLWTH IVHSVHSYGD 

      1570       1580       1590       1600       1610       1620 
NVYMWTHSQP SGNPFTVIIN CLYNSMIMRI VWILLARKLA PEMQSMKKFR ENVSMISYGD 

      1630       1640       1650       1660       1670       1680 
DNCLNISDRV VEWFNQITIS EQMKEIKHEY TDEGKTGDMV KFPSLSEIHF LKKRFVFSHQ 

      1690       1700       1710       1720       1730       1740 
LQRTVAPLQK DVIYEMLNWT RNTIDPNEIL MMNINTAFRE IVYHGKSEYQ KLRSGIEDLA 

      1750       1760       1770 
MKGILPQQPQ ILTFKAYLWD ATMLADEVYD F 

« Hide

References

[1]"Naturally occurring dicistronic cricket paralysis virus RNA is regulated by two internal ribosome entry sites."
Wilson J.E., Powell M.J., Hoover S.E., Sarnow P.
Mol. Cell. Biol. 20:4990-4999(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Cleavage sites of the 'P3 region' in the nonstructural polyprotein precursor of a dicistrovirus."
Nakashima N., Nakamura Y.
Arch. Virol. 153:1955-1960(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEOLYTIC PROCESSING OF POLYPROTEIN.
[3]"Cricket paralysis virus antagonizes Argonaute 2 to modulate antiviral defense in Drosophila."
Nayak A., Berry B., Tassetto M., Kunitomi M., Acevedo A., Deng C., Krutchinsky A., Gross J., Antoniewski C., Andino R.
Nat. Struct. Mol. Biol. 17:547-554(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF PROTEIN 1A, INTERACTION WITH HOST AGO2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF218039 Genomic RNA. Translation: AAF80998.1.
RefSeqNP_647481.1. NC_003924.1.

3D structure databases

ProteinModelPortalQ9IJX4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-59000N.

Proteomic databases

PRIDEQ9IJX4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID944541.

Family and domain databases

InterProIPR004004. Helic/Pol/Pept_Calicivir-typ.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR024387. Pept_C3G_Picornavir.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF12381. Peptidase_C3G. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00910. RNA_helicase. 1 hit.
[Graphical view]
PRINTSPR00918. CALICVIRUSNS.
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePOLN_CRPVC
AccessionPrimary (citable) accession number: Q9IJX4
Entry history
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families