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Q9IJX4

- POLN_CRPVC

UniProt

Q9IJX4 - POLN_CRPVC

Protein

Replicase polyprotein

Gene

ORF1

Organism
Cricket paralysis virus (isolate Teleogryllus commodus/Australia/CrPVVIC/1968) (CrPV)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Protein 1A functions as a suppressor of RNA-mediated gene silencing, an antiviral defense mechanism of insect cells. Inhibits siRNA function, but does not interfere with miRNA pathway. Does not bind to dsRNA or siRNA.1 Publication
    RNA-directed RNA polymerase replicates genomic and antigenomic RNA.1 PublicationPROSITE-ProRule annotation

    Catalytic activityi

    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei166 – 1672Cleavage; by ribosomal skipSequence Analysis
    Sitei190 – 1912Cleavage; by 3C-like proteaseSequence Analysis
    Sitei328 – 3292Cleavage; by 3C-like proteaseSequence Analysis
    Sitei739 – 7402Cleavage; by 3C-like proteaseSequence Analysis
    Sitei884 – 8852Cleavage; by 3C-like proteaseSequence Analysis
    Sitei908 – 9092Cleavage; by 3C-like proteaseSequence Analysis
    Active sitei1162 – 11621Sequence Analysis
    Sitei1220 – 12212Cleavage; by 3C-like proteaseSequence Analysis

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi510 – 5178ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cysteine-type peptidase activity Source: UniProtKB-KW
    3. RNA binding Source: UniProtKB-KW
    4. RNA-directed RNA polymerase activity Source: UniProtKB-KW
    5. RNA helicase activity Source: InterPro

    GO - Biological processi

    1. transcription, DNA-templated Source: InterPro
    2. viral RNA genome replication Source: InterPro

    Keywords - Molecular functioni

    Helicase, Hydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Suppressor of RNA silencing, Thiol protease, Transferase

    Keywords - Biological processi

    Host-virus interaction, Viral RNA replication

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Replicase polyprotein
    Cleaved into the following 8 chains:
    Gene namesi
    ORF Names:ORF1
    OrganismiCricket paralysis virus (isolate Teleogryllus commodus/Australia/CrPVVIC/1968) (CrPV)
    Taxonomic identifieri928300 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stagePicornaviralesDicistroviridaeCripavirus
    Virus hostiTeleogryllus oceanicus (black field cricket) [TaxID: 128161]
    ProteomesiUP000008590: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 908Protein 3BSequence AnalysisPRO_0000398378
    Chaini1 – 17711771Replicase polyproteinPRO_0000398372Add
    BLAST
    Chaini1 – 190190Protein 2ASequence AnalysisPRO_0000398373Add
    BLAST
    Chaini1 – 166166Protein 1ASequence AnalysisPRO_0000398374Add
    BLAST
    Chaini191 – 328138Protein 2BSequence AnalysisPRO_0000398375Add
    BLAST
    Chaini329 – 739411Protein 2CSequence AnalysisPRO_0000398376Add
    BLAST
    Chaini740 – ?Protein 3ASequence AnalysisPRO_0000398377
    Chaini909 – 12203123C-like proteaseSequence AnalysisPRO_0000398379Add
    BLAST
    Chaini1221 – 1771551RNA-directed RNA polymerase 3D-POLSequence AnalysisPRO_0000398380Add
    BLAST

    Post-translational modificationi

    Protein 1A might be expressed through a ribosomal skip from one codon to the next without formation of a peptide bond.

    Proteomic databases

    PRIDEiQ9IJX4.

    Interactioni

    Subunit structurei

    Protein 1A interacts with host AGO2; this interaction may block the RNA-induced silencing complexes (RISC) activity.1 Publication

    Protein-protein interaction databases

    DIPiDIP-59000N.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9IJX4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini482 – 656175SF3 helicasePROSITE-ProRule annotationAdd
    BLAST
    Domaini1495 – 1634140RdRp catalyticPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili1 – 2929Sequence AnalysisAdd
    BLAST
    Coiled coili1385 – 141329Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation
    Contains 1 SF3 helicase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Family and domain databases

    InterProiIPR004004. Helic/Pol/Pept_Calicivir-typ.
    IPR000605. Helicase_SF3_ssDNA/RNA_vir.
    IPR014759. Helicase_SF3_ssRNA_vir.
    IPR024387. Pept_C3G_Picornavir.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF12381. Peptidase_C3G. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF00910. RNA_helicase. 1 hit.
    [Graphical view]
    PRINTSiPR00918. CALICVIRUSNS.
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50507. RDRP_SSRNA_POS. 1 hit.
    PS51218. SF3_HELICASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9IJX4-1 [UniParc]FASTAAdd to Basket

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    MSFQQTNNNA TNNINSLEEL AAQELIAAQF EGNLDGFFCT FYVQSKPQLL     50
    DLESECYCMD DFDCGCDRIK REEELRKLIF LTSDVYGYNF EEWKGLVWKF 100
    VQNYCPEHRY GSTFGNGLLI VSPRFFMDHL DWFQQWKLVS SNDECRAFLR 150
    KRTQLLMSGD VESNPGPVQS RPVYACDNDP RAIRLEKALQ RRDEKISTLI 200
    KKLRQEIKNN RIYTQGFFDD LKGAKGEVGQ LNGNLTRICD FLENSLPTLT 250
    AQIQTTVLTT TDKYVNLKED LLKVAILLVL VRLLMVWKKY RAALIVIILF 300
    VMHFYGFDKQ ILDIVLDLKD KILQTTTQAG TETLEEVVYH PWFDTCGKLI 350
    FAVLAFFAIK KIPGKQDWDN YISRLDRIPK AIEGSKKIVD YCSEYFNLSV 400
    DEVKKVVLGK ELKGTQGLYD EIHVWAKEIR HYLDLDERNK ITLDTETAAK 450
    VEDLYKRGLK YSEEKIPDRD IARFITTMLF PAKSLYEQVL LSPVKGGGPK 500
    MRPITVWLTG ESGIGKTQMI YPLCIDILRE MGIVKPDAYK HQAYARQVET 550
    EYWDGYNGQK IVIYDDAFQL KDDKTKPNPE IFEVIRTCNT FPQHLHMAAL 600
    QDKNMYSQAE VLLYTTNQFQ VQLESITFPD AFYNRMKTHA YRVQIKQEKS 650
    IWVRNARGEE YNALDVTKLN KDEAIDLSVY EFQKMRFDDE SATKWIDDGE 700
    PISYDEFART ICKAWKEEKE KTFHQLQWLE AYASRTVAQG GSETSEYYDV 750
    WDETYFSNLL SQGFMAGKSL IEMEAEFASD AETFNAYIEY KKNIPKETKW 800
    SKWMTILDEQ ISALSTKIRE LKNKAYKFIS EHPYLTALGF IGVMISAFAM 850
    YSFFERTLTD DTITSEVGSS GDNKTQKISK RVVEVGGSGD VKTTKPAKTA 900
    VEVGSSGDSK TMKNKITKVE VGSSGDSKTQ KQRNTKVEVG KELEKEAETQ 950
    GCSDPAAHAL VLDVLQKNTY CLYYERMVKG EMKRYRLATA TFLRGWVCMM 1000
    PYHFIETLYA RKVAPSTNIY FSQPNCDDVI VVPVSHFIAP NAERVELTTA 1050
    CTRIHYKDET PRDCVLVNLH RRMCHPHRDI LKHFVKKSDQ GNLRGVFQGT 1100
    LATFHQSANE LCRAYQWLQA IRPLDQEITI YHEDTDMFDY ESESYTQRDC 1150
    YEYNAPTQTG NCGSIVGLYN KRMERKLIGM HIPGNVSECH GYACPLTQEA 1200
    IMDGLNRLEK LDPVNNITVQ CCFEPPSDIK DTMSGETPEG KFCAIGKSNI 1250
    KVGQAVKTTL LKSCIYGMLS KPITKPAHLT RTRLPNGEIV DPLMKGLKKC 1300
    GVDTAVLDAE IVESAALDVK QVVLTQYNSM LDVNKYRRFL TYEEATQGTG 1350
    DDDFMKGIAR QTSPGYRYFQ MPRKLPGKQD WMGSGEQYDF TSQRAQELRR 1400
    DVEELIDNCA KGIIKDVVFV DTLKDERRPI EKVDAGKTRV FSAGPQHFVV 1450
    AFRKYFLPFA AYLMNNRIDN EIAVGTNVYS TDWERIAKRL KKHGNKVIAG 1500
    DFGNFDGSLV AQFFGQSCGK SFYPWFKTFN DVNTEDGKRN LMICIGLWTH 1550
    IVHSVHSYGD NVYMWTHSQP SGNPFTVIIN CLYNSMIMRI VWILLARKLA 1600
    PEMQSMKKFR ENVSMISYGD DNCLNISDRV VEWFNQITIS EQMKEIKHEY 1650
    TDEGKTGDMV KFPSLSEIHF LKKRFVFSHQ LQRTVAPLQK DVIYEMLNWT 1700
    RNTIDPNEIL MMNINTAFRE IVYHGKSEYQ KLRSGIEDLA MKGILPQQPQ 1750
    ILTFKAYLWD ATMLADEVYD F 1771
    Length:1,771
    Mass (Da):203,830
    Last modified:October 1, 2000 - v1
    Checksum:i78C5969469EAA716
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF218039 Genomic RNA. Translation: AAF80998.1.
    RefSeqiNP_647481.1. NC_003924.1.

    Genome annotation databases

    GeneIDi944541.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF218039 Genomic RNA. Translation: AAF80998.1 .
    RefSeqi NP_647481.1. NC_003924.1.

    3D structure databases

    ProteinModelPortali Q9IJX4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-59000N.

    Proteomic databases

    PRIDEi Q9IJX4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 944541.

    Family and domain databases

    InterProi IPR004004. Helic/Pol/Pept_Calicivir-typ.
    IPR000605. Helicase_SF3_ssDNA/RNA_vir.
    IPR014759. Helicase_SF3_ssRNA_vir.
    IPR024387. Pept_C3G_Picornavir.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF12381. Peptidase_C3G. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF00910. RNA_helicase. 1 hit.
    [Graphical view ]
    PRINTSi PR00918. CALICVIRUSNS.
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50507. RDRP_SSRNA_POS. 1 hit.
    PS51218. SF3_HELICASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Naturally occurring dicistronic cricket paralysis virus RNA is regulated by two internal ribosome entry sites."
      Wilson J.E., Powell M.J., Hoover S.E., Sarnow P.
      Mol. Cell. Biol. 20:4990-4999(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Cleavage sites of the 'P3 region' in the nonstructural polyprotein precursor of a dicistrovirus."
      Nakashima N., Nakamura Y.
      Arch. Virol. 153:1955-1960(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEOLYTIC PROCESSING OF POLYPROTEIN.
    3. "Cricket paralysis virus antagonizes Argonaute 2 to modulate antiviral defense in Drosophila."
      Nayak A., Berry B., Tassetto M., Kunitomi M., Acevedo A., Deng C., Krutchinsky A., Gross J., Antoniewski C., Andino R.
      Nat. Struct. Mol. Biol. 17:547-554(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF PROTEIN 1A, INTERACTION WITH HOST AGO2.

    Entry informationi

    Entry nameiPOLN_CRPVC
    AccessioniPrimary (citable) accession number: Q9IJX4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3