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Q9IJX4

- POLN_CRPVC

UniProt

Q9IJX4 - POLN_CRPVC

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Protein

Replicase polyprotein

Gene

ORF1

Organism
Cricket paralysis virus (isolate Teleogryllus commodus/Australia/CrPVVIC/1968) (CrPV)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Protein 1A functions as a suppressor of RNA-mediated gene silencing, an antiviral defense mechanism of insect cells. Inhibits siRNA function, but does not interfere with miRNA pathway. Does not bind to dsRNA or siRNA.1 Publication
RNA-directed RNA polymerase replicates genomic and antigenomic RNA.1 PublicationPROSITE-ProRule annotation

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei166 – 1672Cleavage; by ribosomal skipSequence Analysis
Sitei190 – 1912Cleavage; by 3C-like proteaseSequence Analysis
Sitei328 – 3292Cleavage; by 3C-like proteaseSequence Analysis
Sitei739 – 7402Cleavage; by 3C-like proteaseSequence Analysis
Sitei884 – 8852Cleavage; by 3C-like proteaseSequence Analysis
Sitei908 – 9092Cleavage; by 3C-like proteaseSequence Analysis
Active sitei1162 – 11621Sequence Analysis
Sitei1220 – 12212Cleavage; by 3C-like proteaseSequence Analysis

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi510 – 5178ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cysteine-type peptidase activity Source: UniProtKB-KW
  3. RNA binding Source: UniProtKB-KW
  4. RNA-directed RNA polymerase activity Source: UniProtKB-KW
  5. RNA helicase activity Source: InterPro

GO - Biological processi

  1. transcription, DNA-templated Source: InterPro
  2. viral RNA genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Suppressor of RNA silencing, Thiol protease, Transferase

Keywords - Biological processi

Host-virus interaction, Viral RNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replicase polyprotein
Cleaved into the following 8 chains:
Gene namesi
ORF Names:ORF1
OrganismiCricket paralysis virus (isolate Teleogryllus commodus/Australia/CrPVVIC/1968) (CrPV)
Taxonomic identifieri928300 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stagePicornaviralesDicistroviridaeCripavirus
Virus hostiTeleogryllus oceanicus (black field cricket) [TaxID: 128161]
ProteomesiUP000008590: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 908Protein 3BSequence AnalysisPRO_0000398378
Chaini1 – 17711771Replicase polyproteinPRO_0000398372Add
BLAST
Chaini1 – 190190Protein 2ASequence AnalysisPRO_0000398373Add
BLAST
Chaini1 – 166166Protein 1ASequence AnalysisPRO_0000398374Add
BLAST
Chaini191 – 328138Protein 2BSequence AnalysisPRO_0000398375Add
BLAST
Chaini329 – 739411Protein 2CSequence AnalysisPRO_0000398376Add
BLAST
Chaini740 – ?Protein 3ASequence AnalysisPRO_0000398377
Chaini909 – 12203123C-like proteaseSequence AnalysisPRO_0000398379Add
BLAST
Chaini1221 – 1771551RNA-directed RNA polymerase 3D-POLSequence AnalysisPRO_0000398380Add
BLAST

Post-translational modificationi

Protein 1A might be expressed through a ribosomal skip from one codon to the next without formation of a peptide bond.

Proteomic databases

PRIDEiQ9IJX4.

Interactioni

Subunit structurei

Protein 1A interacts with host AGO2; this interaction may block the RNA-induced silencing complexes (RISC) activity.1 Publication

Protein-protein interaction databases

DIPiDIP-59000N.

Structurei

3D structure databases

ProteinModelPortaliQ9IJX4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini482 – 656175SF3 helicasePROSITE-ProRule annotationAdd
BLAST
Domaini1495 – 1634140RdRp catalyticPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1 – 2929Sequence AnalysisAdd
BLAST
Coiled coili1385 – 141329Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation
Contains 1 SF3 helicase domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Family and domain databases

InterProiIPR004004. Helic/Pol/Pept_Calicivir-typ.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR024387. Pept_C3G_Picornavir.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF12381. Peptidase_C3G. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00910. RNA_helicase. 1 hit.
[Graphical view]
PRINTSiPR00918. CALICVIRUSNS.
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9IJX4-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MSFQQTNNNA TNNINSLEEL AAQELIAAQF EGNLDGFFCT FYVQSKPQLL
60 70 80 90 100
DLESECYCMD DFDCGCDRIK REEELRKLIF LTSDVYGYNF EEWKGLVWKF
110 120 130 140 150
VQNYCPEHRY GSTFGNGLLI VSPRFFMDHL DWFQQWKLVS SNDECRAFLR
160 170 180 190 200
KRTQLLMSGD VESNPGPVQS RPVYACDNDP RAIRLEKALQ RRDEKISTLI
210 220 230 240 250
KKLRQEIKNN RIYTQGFFDD LKGAKGEVGQ LNGNLTRICD FLENSLPTLT
260 270 280 290 300
AQIQTTVLTT TDKYVNLKED LLKVAILLVL VRLLMVWKKY RAALIVIILF
310 320 330 340 350
VMHFYGFDKQ ILDIVLDLKD KILQTTTQAG TETLEEVVYH PWFDTCGKLI
360 370 380 390 400
FAVLAFFAIK KIPGKQDWDN YISRLDRIPK AIEGSKKIVD YCSEYFNLSV
410 420 430 440 450
DEVKKVVLGK ELKGTQGLYD EIHVWAKEIR HYLDLDERNK ITLDTETAAK
460 470 480 490 500
VEDLYKRGLK YSEEKIPDRD IARFITTMLF PAKSLYEQVL LSPVKGGGPK
510 520 530 540 550
MRPITVWLTG ESGIGKTQMI YPLCIDILRE MGIVKPDAYK HQAYARQVET
560 570 580 590 600
EYWDGYNGQK IVIYDDAFQL KDDKTKPNPE IFEVIRTCNT FPQHLHMAAL
610 620 630 640 650
QDKNMYSQAE VLLYTTNQFQ VQLESITFPD AFYNRMKTHA YRVQIKQEKS
660 670 680 690 700
IWVRNARGEE YNALDVTKLN KDEAIDLSVY EFQKMRFDDE SATKWIDDGE
710 720 730 740 750
PISYDEFART ICKAWKEEKE KTFHQLQWLE AYASRTVAQG GSETSEYYDV
760 770 780 790 800
WDETYFSNLL SQGFMAGKSL IEMEAEFASD AETFNAYIEY KKNIPKETKW
810 820 830 840 850
SKWMTILDEQ ISALSTKIRE LKNKAYKFIS EHPYLTALGF IGVMISAFAM
860 870 880 890 900
YSFFERTLTD DTITSEVGSS GDNKTQKISK RVVEVGGSGD VKTTKPAKTA
910 920 930 940 950
VEVGSSGDSK TMKNKITKVE VGSSGDSKTQ KQRNTKVEVG KELEKEAETQ
960 970 980 990 1000
GCSDPAAHAL VLDVLQKNTY CLYYERMVKG EMKRYRLATA TFLRGWVCMM
1010 1020 1030 1040 1050
PYHFIETLYA RKVAPSTNIY FSQPNCDDVI VVPVSHFIAP NAERVELTTA
1060 1070 1080 1090 1100
CTRIHYKDET PRDCVLVNLH RRMCHPHRDI LKHFVKKSDQ GNLRGVFQGT
1110 1120 1130 1140 1150
LATFHQSANE LCRAYQWLQA IRPLDQEITI YHEDTDMFDY ESESYTQRDC
1160 1170 1180 1190 1200
YEYNAPTQTG NCGSIVGLYN KRMERKLIGM HIPGNVSECH GYACPLTQEA
1210 1220 1230 1240 1250
IMDGLNRLEK LDPVNNITVQ CCFEPPSDIK DTMSGETPEG KFCAIGKSNI
1260 1270 1280 1290 1300
KVGQAVKTTL LKSCIYGMLS KPITKPAHLT RTRLPNGEIV DPLMKGLKKC
1310 1320 1330 1340 1350
GVDTAVLDAE IVESAALDVK QVVLTQYNSM LDVNKYRRFL TYEEATQGTG
1360 1370 1380 1390 1400
DDDFMKGIAR QTSPGYRYFQ MPRKLPGKQD WMGSGEQYDF TSQRAQELRR
1410 1420 1430 1440 1450
DVEELIDNCA KGIIKDVVFV DTLKDERRPI EKVDAGKTRV FSAGPQHFVV
1460 1470 1480 1490 1500
AFRKYFLPFA AYLMNNRIDN EIAVGTNVYS TDWERIAKRL KKHGNKVIAG
1510 1520 1530 1540 1550
DFGNFDGSLV AQFFGQSCGK SFYPWFKTFN DVNTEDGKRN LMICIGLWTH
1560 1570 1580 1590 1600
IVHSVHSYGD NVYMWTHSQP SGNPFTVIIN CLYNSMIMRI VWILLARKLA
1610 1620 1630 1640 1650
PEMQSMKKFR ENVSMISYGD DNCLNISDRV VEWFNQITIS EQMKEIKHEY
1660 1670 1680 1690 1700
TDEGKTGDMV KFPSLSEIHF LKKRFVFSHQ LQRTVAPLQK DVIYEMLNWT
1710 1720 1730 1740 1750
RNTIDPNEIL MMNINTAFRE IVYHGKSEYQ KLRSGIEDLA MKGILPQQPQ
1760 1770
ILTFKAYLWD ATMLADEVYD F
Length:1,771
Mass (Da):203,830
Last modified:October 1, 2000 - v1
Checksum:i78C5969469EAA716
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF218039 Genomic RNA. Translation: AAF80998.1.
RefSeqiNP_647481.1. NC_003924.1.

Genome annotation databases

GeneIDi944541.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF218039 Genomic RNA. Translation: AAF80998.1 .
RefSeqi NP_647481.1. NC_003924.1.

3D structure databases

ProteinModelPortali Q9IJX4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-59000N.

Proteomic databases

PRIDEi Q9IJX4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 944541.

Family and domain databases

InterProi IPR004004. Helic/Pol/Pept_Calicivir-typ.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR024387. Pept_C3G_Picornavir.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF12381. Peptidase_C3G. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00910. RNA_helicase. 1 hit.
[Graphical view ]
PRINTSi PR00918. CALICVIRUSNS.
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Naturally occurring dicistronic cricket paralysis virus RNA is regulated by two internal ribosome entry sites."
    Wilson J.E., Powell M.J., Hoover S.E., Sarnow P.
    Mol. Cell. Biol. 20:4990-4999(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Cleavage sites of the 'P3 region' in the nonstructural polyprotein precursor of a dicistrovirus."
    Nakashima N., Nakamura Y.
    Arch. Virol. 153:1955-1960(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEOLYTIC PROCESSING OF POLYPROTEIN.
  3. "Cricket paralysis virus antagonizes Argonaute 2 to modulate antiviral defense in Drosophila."
    Nayak A., Berry B., Tassetto M., Kunitomi M., Acevedo A., Deng C., Krutchinsky A., Gross J., Antoniewski C., Andino R.
    Nat. Struct. Mol. Biol. 17:547-554(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF PROTEIN 1A, INTERACTION WITH HOST AGO2.

Entry informationi

Entry nameiPOLN_CRPVC
AccessioniPrimary (citable) accession number: Q9IJX4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: October 1, 2000
Last modified: October 1, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3