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Q9IJX4

- POLN_CRPVC

UniProt

Q9IJX4 - POLN_CRPVC

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Protein
Replicase polyprotein
Gene
ORF1
Organism
Cricket paralysis virus (isolate Teleogryllus commodus/Australia/CrPVVIC/1968) (CrPV)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Protein 1A functions as a suppressor of RNA-mediated gene silencing, an antiviral defense mechanism of insect cells. Inhibits siRNA function, but does not interfere with miRNA pathway. Does not bind to dsRNA or siRNA.1 Publication
RNA-directed RNA polymerase replicates genomic and antigenomic RNA.1 Publication

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei166 – 1672Cleavage; by ribosomal skip Reviewed prediction
Sitei190 – 1912Cleavage; by 3C-like protease Reviewed prediction
Sitei328 – 3292Cleavage; by 3C-like protease Reviewed prediction
Sitei739 – 7402Cleavage; by 3C-like protease Reviewed prediction
Sitei884 – 8852Cleavage; by 3C-like protease Reviewed prediction
Sitei908 – 9092Cleavage; by 3C-like protease Reviewed prediction
Active sitei1162 – 11621 Reviewed prediction
Sitei1220 – 12212Cleavage; by 3C-like protease Reviewed prediction

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi510 – 5178ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. RNA binding Source: UniProtKB-KW
  3. RNA helicase activity Source: InterPro
  4. RNA-directed RNA polymerase activity Source: UniProtKB-KW
  5. cysteine-type peptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. transcription, DNA-templated Source: InterPro
  2. viral RNA genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Suppressor of RNA silencing, Thiol protease, Transferase

Keywords - Biological processi

Host-virus interaction, Viral RNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replicase polyprotein
Cleaved into the following 8 chains:
Gene namesi
ORF Names:ORF1
OrganismiCricket paralysis virus (isolate Teleogryllus commodus/Australia/CrPVVIC/1968) (CrPV)
Taxonomic identifieri928300 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stagePicornaviralesDicistroviridaeCripavirus
Virus hostiTeleogryllus oceanicus (black field cricket) [TaxID: 128161]
ProteomesiUP000008590: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 908Protein 3B Reviewed predictionPRO_0000398378
Chaini1 – 17711771Replicase polyprotein
PRO_0000398372Add
BLAST
Chaini1 – 190190Protein 2A Reviewed prediction
PRO_0000398373Add
BLAST
Chaini1 – 166166Protein 1A Reviewed prediction
PRO_0000398374Add
BLAST
Chaini191 – 328138Protein 2B Reviewed prediction
PRO_0000398375Add
BLAST
Chaini329 – 739411Protein 2C Reviewed prediction
PRO_0000398376Add
BLAST
Chaini740 – ?Protein 3A Reviewed predictionPRO_0000398377
Chaini909 – 12203123C-like protease Reviewed prediction
PRO_0000398379Add
BLAST
Chaini1221 – 1771551RNA-directed RNA polymerase 3D-POL Reviewed prediction
PRO_0000398380Add
BLAST

Post-translational modificationi

Protein 1A might be expressed through a ribosomal skip from one codon to the next without formation of a peptide bond.

Proteomic databases

PRIDEiQ9IJX4.

Interactioni

Subunit structurei

Protein 1A interacts with host AGO2; this interaction may block the RNA-induced silencing complexes (RISC) activity.1 Publication

Protein-protein interaction databases

DIPiDIP-59000N.

Structurei

3D structure databases

ProteinModelPortaliQ9IJX4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini482 – 656175SF3 helicase
Add
BLAST
Domaini1495 – 1634140RdRp catalytic
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1 – 2929 Reviewed prediction
Add
BLAST
Coiled coili1385 – 141329 Reviewed prediction
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Family and domain databases

InterProiIPR004004. Helic/Pol/Pept_Calicivir-typ.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR024387. Pept_C3G_Picornavir.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF12381. Peptidase_C3G. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00910. RNA_helicase. 1 hit.
[Graphical view]
PRINTSiPR00918. CALICVIRUSNS.
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9IJX4-1 [UniParc]FASTAAdd to Basket

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MSFQQTNNNA TNNINSLEEL AAQELIAAQF EGNLDGFFCT FYVQSKPQLL     50
DLESECYCMD DFDCGCDRIK REEELRKLIF LTSDVYGYNF EEWKGLVWKF 100
VQNYCPEHRY GSTFGNGLLI VSPRFFMDHL DWFQQWKLVS SNDECRAFLR 150
KRTQLLMSGD VESNPGPVQS RPVYACDNDP RAIRLEKALQ RRDEKISTLI 200
KKLRQEIKNN RIYTQGFFDD LKGAKGEVGQ LNGNLTRICD FLENSLPTLT 250
AQIQTTVLTT TDKYVNLKED LLKVAILLVL VRLLMVWKKY RAALIVIILF 300
VMHFYGFDKQ ILDIVLDLKD KILQTTTQAG TETLEEVVYH PWFDTCGKLI 350
FAVLAFFAIK KIPGKQDWDN YISRLDRIPK AIEGSKKIVD YCSEYFNLSV 400
DEVKKVVLGK ELKGTQGLYD EIHVWAKEIR HYLDLDERNK ITLDTETAAK 450
VEDLYKRGLK YSEEKIPDRD IARFITTMLF PAKSLYEQVL LSPVKGGGPK 500
MRPITVWLTG ESGIGKTQMI YPLCIDILRE MGIVKPDAYK HQAYARQVET 550
EYWDGYNGQK IVIYDDAFQL KDDKTKPNPE IFEVIRTCNT FPQHLHMAAL 600
QDKNMYSQAE VLLYTTNQFQ VQLESITFPD AFYNRMKTHA YRVQIKQEKS 650
IWVRNARGEE YNALDVTKLN KDEAIDLSVY EFQKMRFDDE SATKWIDDGE 700
PISYDEFART ICKAWKEEKE KTFHQLQWLE AYASRTVAQG GSETSEYYDV 750
WDETYFSNLL SQGFMAGKSL IEMEAEFASD AETFNAYIEY KKNIPKETKW 800
SKWMTILDEQ ISALSTKIRE LKNKAYKFIS EHPYLTALGF IGVMISAFAM 850
YSFFERTLTD DTITSEVGSS GDNKTQKISK RVVEVGGSGD VKTTKPAKTA 900
VEVGSSGDSK TMKNKITKVE VGSSGDSKTQ KQRNTKVEVG KELEKEAETQ 950
GCSDPAAHAL VLDVLQKNTY CLYYERMVKG EMKRYRLATA TFLRGWVCMM 1000
PYHFIETLYA RKVAPSTNIY FSQPNCDDVI VVPVSHFIAP NAERVELTTA 1050
CTRIHYKDET PRDCVLVNLH RRMCHPHRDI LKHFVKKSDQ GNLRGVFQGT 1100
LATFHQSANE LCRAYQWLQA IRPLDQEITI YHEDTDMFDY ESESYTQRDC 1150
YEYNAPTQTG NCGSIVGLYN KRMERKLIGM HIPGNVSECH GYACPLTQEA 1200
IMDGLNRLEK LDPVNNITVQ CCFEPPSDIK DTMSGETPEG KFCAIGKSNI 1250
KVGQAVKTTL LKSCIYGMLS KPITKPAHLT RTRLPNGEIV DPLMKGLKKC 1300
GVDTAVLDAE IVESAALDVK QVVLTQYNSM LDVNKYRRFL TYEEATQGTG 1350
DDDFMKGIAR QTSPGYRYFQ MPRKLPGKQD WMGSGEQYDF TSQRAQELRR 1400
DVEELIDNCA KGIIKDVVFV DTLKDERRPI EKVDAGKTRV FSAGPQHFVV 1450
AFRKYFLPFA AYLMNNRIDN EIAVGTNVYS TDWERIAKRL KKHGNKVIAG 1500
DFGNFDGSLV AQFFGQSCGK SFYPWFKTFN DVNTEDGKRN LMICIGLWTH 1550
IVHSVHSYGD NVYMWTHSQP SGNPFTVIIN CLYNSMIMRI VWILLARKLA 1600
PEMQSMKKFR ENVSMISYGD DNCLNISDRV VEWFNQITIS EQMKEIKHEY 1650
TDEGKTGDMV KFPSLSEIHF LKKRFVFSHQ LQRTVAPLQK DVIYEMLNWT 1700
RNTIDPNEIL MMNINTAFRE IVYHGKSEYQ KLRSGIEDLA MKGILPQQPQ 1750
ILTFKAYLWD ATMLADEVYD F 1771
Length:1,771
Mass (Da):203,830
Last modified:October 1, 2000 - v1
Checksum:i78C5969469EAA716
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF218039 Genomic RNA. Translation: AAF80998.1.
RefSeqiNP_647481.1. NC_003924.1.

Genome annotation databases

GeneIDi944541.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF218039 Genomic RNA. Translation: AAF80998.1 .
RefSeqi NP_647481.1. NC_003924.1.

3D structure databases

ProteinModelPortali Q9IJX4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-59000N.

Proteomic databases

PRIDEi Q9IJX4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 944541.

Family and domain databases

InterProi IPR004004. Helic/Pol/Pept_Calicivir-typ.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR024387. Pept_C3G_Picornavir.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF12381. Peptidase_C3G. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00910. RNA_helicase. 1 hit.
[Graphical view ]
PRINTSi PR00918. CALICVIRUSNS.
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Naturally occurring dicistronic cricket paralysis virus RNA is regulated by two internal ribosome entry sites."
    Wilson J.E., Powell M.J., Hoover S.E., Sarnow P.
    Mol. Cell. Biol. 20:4990-4999(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Cleavage sites of the 'P3 region' in the nonstructural polyprotein precursor of a dicistrovirus."
    Nakashima N., Nakamura Y.
    Arch. Virol. 153:1955-1960(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEOLYTIC PROCESSING OF POLYPROTEIN.
  3. "Cricket paralysis virus antagonizes Argonaute 2 to modulate antiviral defense in Drosophila."
    Nayak A., Berry B., Tassetto M., Kunitomi M., Acevedo A., Deng C., Krutchinsky A., Gross J., Antoniewski C., Andino R.
    Nat. Struct. Mol. Biol. 17:547-554(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF PROTEIN 1A, INTERACTION WITH HOST AGO2.

Entry informationi

Entry nameiPOLN_CRPVC
AccessioniPrimary (citable) accession number: Q9IJX4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: October 1, 2000
Last modified: May 14, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3