Q9IH62 (GLYCP_NIPAV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycoprotein G

Gene names

Name:

G

Organism

Nipah virus [Complete proteome]

Taxonomic identifier

121791 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Mononegavirales › Paramyxoviridae › Paramyxovirinae › Henipavirus

Virus host

Pteropus hypomelanus (Island flying fox) (Variable flying fox) [TaxID: 9405]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]
Cynopterus brachyotis (Lesser short-nosed fruit bat) (Pachysoma brachyotis) [TaxID: 58060]
Eonycteris spelaea (Lesser dawn bat) (Macroglossus spelaeus) [TaxID: 58065]
Pteropus vampyrus (Large flying fox) [TaxID: 132908]
Scotophilus kuhlii (Lesser asiatic yellow bat) [TaxID: 153297]

Protein attributes

Sequence length	602 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Interacts with host ephrinB2/EFNB2 or ephrin B3/EFNB3 to provide virion attachment to target cell. This attachment induces virion internalization predominantly through clathrin-mediated endocytosis. Ref.9 Ref.10
Subunit structure	Interacts with host EFNB2 and host EFNB3. Ref.7 Ref.8
Subcellular location	Virion membrane; Single-pass type II membrane protein Potential. Host cell membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the paramyxoviruses hemagglutinin-neuraminidase family.
Caution	Henipavirus glycoproteins have no neuraminidase activity.

Ontologies

Keywords
Biological process	Clathrin-mediated endocytosis of virus by host Host-virus interaction Initiation of viral infection Viral attachment to host cell Viral penetration into host cytoplasm Virus endocytosis by host
Cellular component	Host cell membrane Host membrane Membrane Viral envelope protein Virion
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Hemagglutinin
PTM	Glycoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	interspecies interaction between organisms Inferred from electronic annotation. Source: UniProtKB-KW viral infectious cycle Inferred from electronic annotation. Source: InterPro
Cellular component	host cell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW viral envelope Inferred from electronic annotation. Source: InterPro virion membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	exo-alpha-sialidase activity Inferred from electronic annotation. Source: InterPro host cell surface receptor binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 602

602

Glycoprotein G

PRO_0000236009

Regions

Topological domain

1 – 49

49

Cytoplasmic Potential

Transmembrane

50 – 70

21

Helical; Potential

Topological domain

71 – 602

532

Extracellular Potential

Amino acid modifications

Glycosylation

72

1

N-linked (GlcNAc...); by host Potential

Glycosylation

159

1

N-linked (GlcNAc...); by host Potential

Glycosylation

306

1

N-linked (GlcNAc...); by host Potential

Glycosylation

378

1

N-linked (GlcNAc...); by host Potential

Glycosylation

417

1

N-linked (GlcNAc...); by host Potential

Glycosylation

481

1

N-linked (GlcNAc...); by host Potential

Glycosylation

529

1

N-linked (GlcNAc...); by host Potential

Natural variations

Natural variant

5

1

N → S in strain: Isolate NiV/KHM/CSUR38.

Natural variant

20

1

I → N in strain: Isolate NiV/MY/99/VRI-0626.

Natural variant

24

1

V → I in strain: Isolate NiV/KHM/CSUR38.

Natural variant

248

1

R → K in strain: Isolate NiV/KHM/CSUR38.

Natural variant

272

1

T → A in strain: Isolate NiV/MY/99/VRI-0626.

Natural variant

327

1

G → D in strain: Isolate NiV/KHM/CSUR38.

Natural variant

408

1

I → V in strain: Isolate NiV/KHM/CSUR38.

Natural variant

426

1

V → I in strain: Isolate NiV/KHM/CSUR38.

Natural variant

470

1

L → Q in strain: Isolate NiV/KHM/CSUR38.

Natural variant

478

1

N → S in strain: Isolate NiV/KHM/CSUR38.

Natural variant

481

1

N → D in strain: Isolate NiV/KHM/CSUR38.

Secondary structure

1

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602

Helix Strand Turn

Details...

Beta strand

201 – 203

3

Beta strand

216 – 224

9

Beta strand

229 – 236

8

Beta strand

245 – 250

6

Beta strand

255 – 257

3

Beta strand

263 – 265

3

Beta strand

278 – 287

10

Beta strand

290 – 297

8

Turn

303 – 305

3

Turn

307 – 309

3

Beta strand

314 – 322

9

Beta strand

332 – 336

5

Beta strand

361 – 364

4

Helix

372 – 374

3

Helix

379 – 381

3

Helix

394 – 397

4

Beta strand

406 – 408

3

Beta strand

414 – 417

4

Beta strand

426 – 428

3

Beta strand

442 – 446

5

Beta strand

451 – 455

5

Beta strand

469 – 471

3

Turn

472 – 474

3

Beta strand

475 – 477

3

Beta strand

512 – 515

4

Turn

516 – 519

4

Beta strand

520 – 522

3

Beta strand

529 – 533

5

Beta strand

535 – 541

7

Beta strand

544 – 550

7

Beta strand

557 – 568

12

Beta strand

571 – 581

11

Beta strand

591 – 596

6

Sequences

	Sequence		Length	Mass (Da)
	Q9IH62 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 3BD2A917D30A73BE	FASTA	602	67,039
	10 20 30 40 50 60 MPAENKKVRF ENTTSDKGKI PSKVIKSYYG TMDIKKINEG LLDSKILSAF NTVIALLGSI 70 80 90 100 110 120 VIIVMNIMII QNYTRSTDNQ AVIKDALQGI QQQIKGLADK IGTEIGPKVS LIDTSSTITI 130 140 150 160 170 180 PANIGLLGSK ISQSTASINE NVNEKCKFTL PPLKIHECNI SCPNPLPFRE YRPQTEGVSN 190 200 210 220 230 240 LVGLPNNICL QKTSNQILKP KLISYTLPVV GQSGTCITDP LLAMDEGYFA YSHLERIGSC 250 260 270 280 290 300 SRGVSKQRII GVGEVLDRGD EVPSLFMTNV WTPPNPNTVY HCSAVYNNEF YYVLCAVSTV 310 320 330 340 350 360 GDPILNSTYW SGSLMMTRLA VKPKSNGGGY NQHQLALRSI EKGRYDKVMP YGPSGIKQGD 370 380 390 400 410 420 TLYFPAVGFL VRTEFKYNDS NCPITKCQYS KPENCRLSMG IRPNSHYILR SGLLKYNLSD 430 440 450 460 470 480 GENPKVVFIE ISDQRLSIGS PSKIYDSLGQ PVFYQASFSW DTMIKFGDVL TVNPLVVNWR 490 500 510 520 530 540 NNTVISRPGQ SQCPRFNTCP EICWEGVYND AFLIDRINWI SAGVFLDSNQ TAENPVFTVF 550 560 570 580 590 600 KDNEILYRAQ LASEDTNAQK TITNCFLLKN KIWCISLVEI YDTGDNVIRP KLFAVKIPEQ CT

References

[1]	"Molecular characterization of Nipah virus, a newly emergent paramyxovirus." Harcourt B.H., Tamin A., Rollin P.E., Ksiazek T.G., Anderson L.J., Bellini W.J., Rota P.A. Virology 271:334-349(2000) [PubMed: 10860887] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Nipah virus: a recently emergent deadly paramyxovirus." Chua K.B., Bellini W.J., Rota P.A., Harcourt B.H., Tamin A., Lam S.K., Ksiazek T.G., Rollin P.E., Zaki S.R., Shieh W., Goldsmith C.S., Gubler D.J., Roehrig J.T., Eaton B., Gould A.R., Olson J., Field H., Daniels P. , Ling A.E., Peters C.J., Anderson L.J., Mahy B.W. Science 288:1432-1435(2000) [PubMed: 10827955] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[3]	"Complete nucleotide sequences of Nipah virus isolates from Malaysia." Chan Y.P., Chua K.B., Koh C.L., Lim M.E., Lam S.K. J. Gen. Virol. 82:2151-2155(2001) [PubMed: 11514724] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate UMMC1 and Isolate UMMC2.
[4]	"Isolation of Nipah virus from Malaysian island flying-foxes." Chua K.B., Koh C.L., Hooi P.S., Wee K.F., Khong J.H., Chua B.H., Chan Y.P., Lim M.E., Lam S.K. Microbes Infect. 4:145-151(2002) [PubMed: 11880045] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate Malaysian flying-fox.
[5]	"Isolation and molecular identification of Nipah virus from pigs." Abubakar S., Chang L.Y., Mohdali A.R., Sharifah S.H., Yusoff K., Zamrod Z. Emerg. Infect. Dis. 10:2228-2230(2004) [PubMed: 15663869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate NiV/MY/99/UM-0128, Isolate NiV/MY/99/VRI-1413 and Isolate NiV/MY/99/VRI-2794.
[6]	"Nipah virus in Lyle's flying foxes, Cambodia." Reynes J.-M., Counor D., Ong S., Faure C., Seng V., Molia S., Walston J., Georges-Courbot M., Deubel V., Sarthou J.-L. Emerg. Infect. Dis. 11:1042-1047(2005) [PubMed: 16022778] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate NiV/KHM/CSUR381.
[7]	"EphrinB2 is the entry receptor for Nipah virus, an emergent deadly paramyxovirus." Negrete O.A., Levroney E.L., Aguilar H.C., Bertolotti-Ciarlet A., Nazarian R., Tajyar S., Lee B. Nature 436:401-405(2005) [PubMed: 16007075] [Abstract] Cited for: INTERACTION WITH HOST EFNB2.
[8]	"Two key residues in ephrinB3 are critical for its use as an alternative receptor for Nipah virus." Negrete O.A., Wolf M.C., Aguilar H.C., Enterlein S., Wang W., Muehlberger E., Su S.V., Bertolotti-Ciarlet A., Flick R., Lee B. PLoS Pathog. 2:78-86(2006) [PubMed: 16477309] [Abstract] Cited for: INTERACTION WITH HOST EFNB3.
[9]	"Molecular characteristics of the Nipah virus glycoproteins." Diederich S., Maisner A. Ann. N. Y. Acad. Sci. 1102:39-50(2007) [PubMed: 17470910] [Abstract] Cited for: FUNCTION.
[10]	"The nipah virus fusion protein is cleaved within the endosomal compartment." Diederich S., Moll M., Klenk H.D., Maisner A. J. Biol. Chem. 280:29899-29903(2005) [PubMed: 15961384] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF238467 mRNA. Translation: AAF73957.1.
AF212302 Genomic RNA. Translation: AAK29088.1.
AY029767 Genomic RNA. Translation: AAK50545.1.
AF376747 Genomic RNA. Translation: AAM13406.1.
AY029768 Genomic RNA. Translation: AAK50554.1.
AJ564621 Genomic RNA. Translation: CAD92351.1.
AJ564622 Genomic RNA. Translation: CAD92357.1.
AJ564623 Genomic RNA. Translation: CAD92363.1.
AJ627196 Genomic RNA. Translation: CAF25497.1.
AY858111 Genomic RNA. Translation: AAX51853.1.

RefSeq

NP_112027.1. NC_002728.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2VSM	X-ray	1.80	A	188-602	[»]
2VWD	X-ray	2.25	A/B	183-602	[»]
3D11	X-ray	2.31	A	176-602	[»]
3D12	X-ray	3.00	A/D	176-602	[»]

ProteinModelPortal

Q9IH62.

SMR

Q9IH62. Positions 176-602.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-46380N.

Protein family/group databases

CAZy

GH83. Glycoside Hydrolase Family 83.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

920955.

Family and domain databases

InterPro

IPR000665. Hemagglutn-neuramid_glycoprot.
IPR016285. Hemagglutn-neuramid_paramyxo.
IPR011040. Neuraminidase.
[Graphical view]

Gene3D

G3DSA:2.120.10.10. Neuraminidase. 1 hit.

Pfam

PF00423. HN. 1 hit.
[Graphical view]

PIRSF

PIRSF001072. Hemagglut-neuramid_paramyxoV. 1 hit.

SUPFAM

SSF50939. Sialidase. 1 hit.

ProtoNet

Search...

Entry information

Entry name

GLYCP_NIPAV

Accession

Primary (citable) accession number: Q9IH62
Secondary accession number(s): Q4KTA8, Q5K4D8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program