Reviewed, UniProtKB/Swiss-Prot Q9IH62 (GLYCP_NIPAV)

Names and origin

Protein names

Recommended name:
    Glycoprotein G

Gene names

Name:

G

Organism

Nipah virus

Taxonomic identifier

121791 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Mononegavirales › Paramyxoviridae › Paramyxovirinae › Henipavirus

Virus host

Pteropus hypomelanus (Island flying fox) (Variable flying fox) [TaxID: 9405]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]
Cynopterus brachyotis (Lesser short-nosed fruit bat) [TaxID: 58060]
Eonycteris spelaea (Lesser dawn bat) [TaxID: 58065]
Pteropus vampyrus (Large flying fox) [TaxID: 132908]
Scotophilus kuhlii (Lesser asiatic yellow house bat) [TaxID: 153297]

Protein attributes

Sequence length	602 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Interacts with host ephrinB2/EFNB2 or ephrin B3/EFNB3 to provide virion attachment to target cell. Ref.9
Subunit structure	Interacts with host EFNB2 and host EFNB3. Ref.7 Ref.8
Subcellular location	Virion membrane; Single-pass type II membrane protein Potential. Host cell membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the paramyxoviruses hemagglutinin-neuraminidase family.
Caution	Henipavirus glycoproteins have no neuraminidase activity.

Ontologies

Keywords
Biological process	Host-virus interaction
Cellular component	Cell membrane Envelope protein Membrane Virion
Domain	Signal-anchor Transmembrane
Molecular function	Hemagglutinin
PTM	Glycoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	interspecies interaction between organisms Inferred from electronic annotation. Source: UniProtKB-KW viral infectious cycle Inferred from electronic annotation. Source: InterPro
Cellular component	host cell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW viral envelope Inferred from electronic annotation. Source: UniProtKB-KW virion membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	exo-alpha-sialidase activity Inferred from electronic annotation. Source: InterPro host cell surface receptor binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 602	602	Glycoprotein G		PRO_0000236009
Regions
	Topological domain	1 – 49	49	Cytoplasmic Potential
	Transmembrane	50 – 70	21	Potential
	Topological domain	71 – 602	532	Extracellular Potential
Amino acid modifications
	Glycosylation	72	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	159	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	306	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	378	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	417	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	481	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	529	1	N-linked (GlcNAc...); by host Potential
Natural variations
	Natural variant	5	1	N → S in strain: Isolate NiV/KHM/CSUR38.
	Natural variant	20	1	I → N in strain: Isolate NiV/MY/99/VRI-0626.
	Natural variant	24	1	V → I in strain: Isolate NiV/KHM/CSUR38.
	Natural variant	248	1	R → K in strain: Isolate NiV/KHM/CSUR38.
	Natural variant	272	1	T → A in strain: Isolate NiV/MY/99/VRI-0626.
	Natural variant	327	1	G → D in strain: Isolate NiV/KHM/CSUR38.
	Natural variant	408	1	I → V in strain: Isolate NiV/KHM/CSUR38.
	Natural variant	426	1	V → I in strain: Isolate NiV/KHM/CSUR38.
	Natural variant	470	1	L → Q in strain: Isolate NiV/KHM/CSUR38.
	Natural variant	478	1	N → S in strain: Isolate NiV/KHM/CSUR38.
	Natural variant	481	1	N → D in strain: Isolate NiV/KHM/CSUR38.

Sequences

	Sequence		Length	Mass (Da)
	Q9IH62-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 3BD2A917D30A73BE	FASTA	602	67,039
	10 20 30 40 50 60 MPAENKKVRF ENTTSDKGKI PSKVIKSYYG TMDIKKINEG LLDSKILSAF NTVIALLGSI 70 80 90 100 110 120 VIIVMNIMII QNYTRSTDNQ AVIKDALQGI QQQIKGLADK IGTEIGPKVS LIDTSSTITI 130 140 150 160 170 180 PANIGLLGSK ISQSTASINE NVNEKCKFTL PPLKIHECNI SCPNPLPFRE YRPQTEGVSN 190 200 210 220 230 240 LVGLPNNICL QKTSNQILKP KLISYTLPVV GQSGTCITDP LLAMDEGYFA YSHLERIGSC 250 260 270 280 290 300 SRGVSKQRII GVGEVLDRGD EVPSLFMTNV WTPPNPNTVY HCSAVYNNEF YYVLCAVSTV 310 320 330 340 350 360 GDPILNSTYW SGSLMMTRLA VKPKSNGGGY NQHQLALRSI EKGRYDKVMP YGPSGIKQGD 370 380 390 400 410 420 TLYFPAVGFL VRTEFKYNDS NCPITKCQYS KPENCRLSMG IRPNSHYILR SGLLKYNLSD 430 440 450 460 470 480 GENPKVVFIE ISDQRLSIGS PSKIYDSLGQ PVFYQASFSW DTMIKFGDVL TVNPLVVNWR 490 500 510 520 530 540 NNTVISRPGQ SQCPRFNTCP EICWEGVYND AFLIDRINWI SAGVFLDSNQ TAENPVFTVF 550 560 570 580 590 600 KDNEILYRAQ LASEDTNAQK TITNCFLLKN KIWCISLVEI YDTGDNVIRP KLFAVKIPEQ CT

References

[1]	"Molecular characterization of Nipah virus, a newly emergent paramyxovirus." Harcourt B.H., Tamin A., Rollin P.E., Ksiazek T.G., Anderson L.J., Bellini W.J., Rota P.A. Virology 271:334-349(2000) [PubMed: 10860887] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Nipah virus: a recently emergent deadly paramyxovirus." Chua K.B., Bellini W.J., Rota P.A., Harcourt B.H., Tamin A., Lam S.K., Ksiazek T.G., Rollin P.E., Zaki S.R., Shieh W., Goldsmith C.S., Gubler D.J., Roehrig J.T., Eaton B., Gould A.R., Olson J., Field H., Daniels P. , Ling A.E., Peters C.J., Anderson L.J., Mahy B.W. Science 288:1432-1435(2000) [PubMed: 10827955] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[3]	"Complete nucleotide sequences of Nipah virus isolates from Malaysia." Chan Y.P., Chua K.B., Koh C.L., Lim M.E., Lam S.K. J. Gen. Virol. 82:2151-2155(2001) [PubMed: 11514724] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate UMMC1 and Isolate UMMC2.
[4]	"Isolation of Nipah virus from Malaysian island flying-foxes." Chua K.B., Koh C.L., Hooi P.S., Wee K.F., Khong J.H., Chua B.H., Chan Y.P., Lim M.E., Lam S.K. Microbes Infect. 4:145-151(2002) [PubMed: 11880045] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate Malaysian flying-fox.
[5]	"Isolation and molecular identification of Nipah virus from pigs." Abubakar S., Chang L.Y., Mohdali A.R., Sharifah S.H., Yusoff K., Zamrod Z. Emerg. Infect. Dis. 10:2228-2230(2004) [PubMed: 15663869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate NiV/MY/99/UM-0128, Isolate NiV/MY/99/VRI-1413 and Isolate NiV/MY/99/VRI-2794.
[6]	"Nipah virus in Lyle's flying foxes, Cambodia." Reynes J.-M., Counor D., Ong S., Faure C., Seng V., Molia S., Walston J., Georges-Courbot M., Deubel V., Sarthou J.-L. Emerg. Infect. Dis. 11:1042-1047(2005) [PubMed: 16022778] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate NiV/KHM/CSUR381.
[7]	"EphrinB2 is the entry receptor for Nipah virus, an emergent deadly paramyxovirus." Negrete O.A., Levroney E.L., Aguilar H.C., Bertolotti-Ciarlet A., Nazarian R., Tajyar S., Lee B. Nature 436:401-405(2005) [PubMed: 16007075] [Abstract] Cited for: INTERACTION WITH HOST EFNB2.
[8]	"Two key residues in ephrinB3 are critical for its use as an alternative receptor for Nipah virus." Negrete O.A., Wolf M.C., Aguilar H.C., Enterlein S., Wang W., Muehlberger E., Su S.V., Bertolotti-Ciarlet A., Flick R., Lee B. PLoS Pathog. 2:78-86(2006) [PubMed: 16477309] [Abstract] Cited for: INTERACTION WITH HOST EFNB3.
[9]	"Molecular characteristics of the Nipah virus glycoproteins." Diederich S., Maisner A. Ann. N. Y. Acad. Sci. 1102:39-50(2007) [PubMed: 17470910] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF238467 mRNA. Translation: AAF73957.1.
AF212302 Genomic RNA. Translation: AAK29088.1.
AY029767 Genomic RNA. Translation: AAK50545.1.
AF376747 Genomic RNA. Translation: AAM13406.1.
AY029768 Genomic RNA. Translation: AAK50554.1.
AJ564621 Genomic RNA. Translation: CAD92351.1.
AJ564622 Genomic RNA. Translation: CAD92357.1.
AJ564623 Genomic RNA. Translation: CAD92363.1.
AJ627196 Genomic RNA. Translation: CAF25497.1.
AY858111 Genomic RNA. Translation: AAX51853.1.

RefSeq

NP_112027.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2VSM	X-ray	1.80	A	188-602	[»]
2VWD	X-ray	2.25	A/B	183-602	[»]
3D11	X-ray	2.31	A	176-602	[»]
3D12	X-ray	3.00	A/D	176-602	[»]

ModBase

Search...

Protein family/group databases

CAZy

GH83. Glycoside Hydrolase Family 83.

Genome annotation databases

GeneID

920955.

Enzyme and pathway databases

BRENDA

3.2.1.18. 294886.

Family and domain databases

InterPro

IPR000665. Hemagglutn-neuramid_glycoprot.
IPR016285. Hemagglutn-neuramid_paramyxo.
[Graphical view]

Pfam

PF00423. HN. 1 hit.
[Graphical view]

PIRSF

PIRSF001072. Hemagglut-neuramid_paramyxoV. 1 hit.

ProtoNet

Search...

Entry information

Entry name

GLYCP_NIPAV

Accession

Primary (citable) accession number: Q9IH62
Secondary accession number(s): Q4KTA8, Q5K4D8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)