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Q9IGQ0

- NRAM_I76AI

UniProt

Q9IGQ0 - NRAM_I76AI

Protein

Neuraminidase

Gene

NA

Organism
Influenza A virus (strain A/Swine/New Jersey/11/1976 H1N1)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Catalyzes the removal of terminal sialic acid residues from viral and cellular glycoconjugates. Cleaves off the terminal sialic acids on the glycosylated HA during virus budding to facilitate virus release. Additionally helps virus spread through the circulation by further removing sialic acids from the cell surface. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell. Otherwise, infection would be limited to one round of replication. Described as a receptor-destroying enzyme because it cleaves a terminal sialic acid from the cellular receptors. May facilitate viral invasion of the upper airways by cleaving the sialic acid moities on the mucin of the airway epithelial cells. Likely to plays a role in the budding process through its association with lipid rafts during intracellular transport. May additionally display a raft-association independent effect on budding. Plays a role in the determination of host range restriction on replication and virulence. Sialidase activity in late endosome/lysosome traffic seems to enhance virus replication.

    Catalytic activityi

    Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

    Cofactori

    Binds 1 calcium ion per subunit.By similarity

    Enzyme regulationi

    Inhibited by the neuraminidase inhibitors zanamivir (Relenza) and oseltamivir (Tamiflu). These drugs interfere with the release of progeny virus from infected cells and are effective against all influenza strains. Resistance to neuraminidase inhibitors is quite rare.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei118 – 1181SubstrateBy similarity
    Active sitei151 – 1511Proton donor/acceptorBy similarity
    Binding sitei152 – 1521SubstrateBy similarity
    Binding sitei293 – 2931SubstrateBy similarity
    Metal bindingi294 – 2941Calcium; via carbonyl oxygenBy similarity
    Metal bindingi324 – 3241CalciumBy similarity
    Binding sitei368 – 3681SubstrateBy similarity
    Active sitei402 – 4021NucleophileBy similarity

    GO - Molecular functioni

    1. exo-alpha-(2->3)-sialidase activity Source: UniProtKB-EC
    2. exo-alpha-(2->6)-sialidase activity Source: UniProtKB-EC
    3. exo-alpha-(2->8)-sialidase activity Source: UniProtKB-EC
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Calcium, Metal-binding

    Protein family/group databases

    CAZyiGH34. Glycoside Hydrolase Family 34.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Neuraminidase (EC:3.2.1.18)
    Gene namesi
    Name:NA
    OrganismiInfluenza A virus (strain A/Swine/New Jersey/11/1976 H1N1)
    Taxonomic identifieri186460 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
    Virus hostiAves [TaxID: 8782]
    Homo sapiens (Human) [TaxID: 9606]
    Sus scrofa (Pig) [TaxID: 9823]

    Subcellular locationi

    Virion membrane By similarity. Host apical cell membrane By similarity; Single-pass type II membrane protein By similarity
    Note: Preferentially accumulates at the apical plasma membrane in infected polarized epithelial cells, which is the virus assembly site. Uses lipid rafts for cell surface transport and apical sorting. In the virion, forms a mushroom-shaped spike on the surface of the membrane By similarity.By similarity

    GO - Cellular componenti

    1. host cell plasma membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. virion membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cell membrane, Host membrane, Membrane, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 469469NeuraminidasePRO_0000280159Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi50 – 501N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi58 – 581N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi63 – 631N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi68 – 681N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi88 – 881N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi92 ↔ 417By similarity
    Disulfide bondi124 ↔ 129By similarity
    Glycosylationi146 – 1461N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi184 ↔ 231By similarity
    Disulfide bondi233 ↔ 238By similarity
    Glycosylationi235 – 2351N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi279 ↔ 292By similarity
    Disulfide bondi281 ↔ 290By similarity
    Disulfide bondi318 ↔ 335By similarity
    Disulfide bondi421 ↔ 446By similarity

    Post-translational modificationi

    N-glycosylated.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ9IGQ0.
    SMRiQ9IGQ0. Positions 83-467.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1212IntravirionSequence AnalysisAdd
    BLAST
    Topological domaini34 – 469436Virion surfaceSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei13 – 3321Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni11 – 3323Involved in apical transport and lipid raft associationBy similarityAdd
    BLAST
    Regioni36 – 9055Hypervariable stalk regionBy similarityAdd
    BLAST
    Regioni91 – 469379Head of neuraminidaseBy similarityAdd
    BLAST
    Regioni277 – 2782Substrate bindingBy similarity

    Domaini

    Intact N-terminus is essential for virion morphogenesis. Possess two apical sorting signals, one in the ectodomain, which is likely to be a glycan, and the other in the transmembrane domain. The transmembrane domain also plays a role in lipid raft association By similarity.By similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 34 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Family and domain databases

    Gene3Di2.120.10.10. 1 hit.
    InterProiIPR001860. Glyco_hydro_34.
    IPR011040. Sialidases.
    [Graphical view]
    PfamiPF00064. Neur. 1 hit.
    [Graphical view]
    SUPFAMiSSF50939. SSF50939. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9IGQ0-1 [UniParc]FASTAAdd to Basket

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    MNTNQRIITI GTICLIVGII SLLLQIGNII LLWMSHSIQT GEKSHPKVCN    50
    QSVITYENNT WVNQTYVNIS NTNIAAGQGV TPIILAGNSS LCPISGWAIY 100
    SKDNSIRIGS KGDIFVMREP FISCSHLECR TFFLTQGALL NDRHSNGTVK 150
    DRSPYRTLMS CPIGEAPSPY NSRFESVAWS ASACHDGMGW LTIGISGPDN 200
    GAVAVLKYNG IITDTIKSWR NKILRTQESE CVCINGSCFT IMTDGPSNGQ 250
    ASYKLFKMEK GKIIRSIELD APNYHYEECS CYPDTGKVVC VCRDNWHASN 300
    RPWVSFDQNL DYQIGYICSG VFGDNPRSND GKGNCGPVLS NGANGVKGFS 350
    FRYGNGVWIG RTKSISSRSG FEMIWDPNGW TETDSSFSMK QDIIALTDWS 400
    GYSGSFVQHP ELTGMNCIRP CFWVELIRGQ PKESTIWTSG SSISFCGVNS 450
    GTASWSWPDG ADLPFTIDK 469
    Length:469
    Mass (Da):51,620
    Last modified:October 1, 2000 - v1
    Checksum:iC56DCFFAA7121C96
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF250363 mRNA. Translation: AAF77043.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF250363 mRNA. Translation: AAF77043.1 .

    3D structure databases

    ProteinModelPortali Q9IGQ0.
    SMRi Q9IGQ0. Positions 83-467.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH34. Glycoside Hydrolase Family 34.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.120.10.10. 1 hit.
    InterProi IPR001860. Glyco_hydro_34.
    IPR011040. Sialidases.
    [Graphical view ]
    Pfami PF00064. Neur. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50939. SSF50939. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of the 1918 'Spanish' influenza virus neuraminidase gene."
      Reid A.H., Fanning T.G., Janczewski T.A., Taubenberger J.K.
      Proc. Natl. Acad. Sci. U.S.A. 97:6785-6790(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Assembly and budding of influenza virus."
      Nayak D.P., Hui E.K., Barman S.
      Virus Res. 106:147-165(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.
    3. "Neuraminidase inhibitors for influenza."
      Moscona A.
      N. Engl. J. Med. 353:1363-1373(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.
    4. "Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses."
      Suzuki Y.
      Biol. Pharm. Bull. 28:399-408(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.

    Entry informationi

    Entry nameiNRAM_I76AI
    AccessioniPrimary (citable) accession number: Q9IGQ0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 6, 2007
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The influenza A genome consist of 8 RNA segments. Genetic variation of hemagglutinin and/or neuraminidase genes results in the emergence of new influenza strains. The mechanism of variation can be the result of point mutations or the result of genetic reassortment between segments of two different strains.

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3