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Q9IG45 (REP_PICV) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Replication-associated protein

EC=2.7.7.-
EC=3.1.21.-
EC=3.6.1.3
Alternative name(s):
ATP-dependent helicase Rep
RepP
Gene names
Name:rep
OrganismPigeon circovirus (PiCV) (Columbid circovirus) [Complete proteome]
Taxonomic identifier126070 [NCBI]
Taxonomic lineageVirusesssDNA virusesCircoviridaeCircovirusunclassified Circovirus
Virus hostColumba livia (Domestic pigeon) [TaxID: 8932]

Protein attributes

Sequence length317 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Essential for the replication of viral ssDNA. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep and/or Rep' binds a specific hairpin at the genome origin of replication. Introduces an endonucleolytic nick within the conserved sequence 5'-AGTATTAC-3' in the intergenic region of the genome, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities. ATPase activity is probably carried by the isoform Rep By similarity.

Catalytic activity

ATP + H2O = ADP + phosphate.

Cofactor

Divalent metal cations, possibly magnesium or manganese Probable.

Subunit structure

Interacts with the capsid protein; this interaction relocates Rep into the nucleus By similarity.

Subcellular location

Host nucleus Potential.

Domain

There are 3 rolling circle replication (RCR) motifs. RCR-2 is probably involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR.

Sequence similarities

Belongs to the nanoviruses/circoviruses replication-associated protein family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 317317Replication-associated protein
PRO_0000319865

Regions

Nucleotide binding192 – 1998ATP By similarity
Motif5 – 4036Nuclear localization signal Potential
Motif41 – 444RCR-1 By similarity
Motif78 – 836RCR-2 By similarity
Motif87 – 10721Nuclear localization signal Potential
Motif116 – 1194RCR-3 By similarity

Sites

Active site1161For DNA cleavage activity By similarity
Metal binding701Divalent metal cation Potential
Metal binding781Divalent metal cation Potential
Metal binding1201Divalent metal cation Potential

Sequences

Sequence LengthMass (Da)Tools
Q9IG45 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 988D75198E300EE6

FASTA31736,254
        10         20         30         40         50         60 
MAPCKPGSNP PKGRVSAAEG GARREATRRP PREAAAKRWC FTLNNPTEEE IKSLETWLVS 

        70         80         90        100        110        120 
DFHYAIVGKE VGEQGTPHLQ GFVHLKQKKR LPQLKQLFKR AHWEKARGSD EDNEKYCSKE 

       130        140        150        160        170        180 
GNVLLTLGIP AKGNRSDLSE AVAAVKAGRA MTEVARDFSE IYVKYGRGLR DLKLLIGQQP 

       190        200        210        220        230        240 
RDFKTEVIVI TGPPGCGKSR WAADYPGSKF YKMKGEWWDG YDHQEVVIID DFYGWLPFCE 

       250        260        270        280        290        300 
LLRVTDRYPH KVPVKGAFVE FTSRVIIVTS NSPPDAWYSE ERCCVQALFR RINKWLVWNH 

       310 
DKFEDAPDCM KKYPINY 

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References

[1]"Cloning and sequencing of columbid circovirus (coCV), a new circovirus from pigeons."
Mankertz A., Hattermann K., Ehlers B., Soike D.
Arch. Virol. 145:2469-2479(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF252610 Genomic DNA. Translation: AAF74196.1.
RefSeqNP_059527.1. NC_002361.1.

3D structure databases

ProteinModelPortalQ9IG45.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1460801.

Family and domain databases

InterProIPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR027417. P-loop_NTPase.
IPR003365. Viral_rep_N.
[Graphical view]
PfamPF00910. RNA_helicase. 1 hit.
PF02407. Viral_Rep. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
ProtoNetSearch...

Entry information

Entry nameREP_PICV
AccessionPrimary (citable) accession number: Q9IG45
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families