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Q9IG45

- REP_PICV

UniProt

Q9IG45 - REP_PICV

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Protein

Replication-associated protein

Gene

rep

Organism
Pigeon circovirus (PiCV) (Columbid circovirus)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Essential for the replication of viral ssDNA. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep and/or Rep' binds a specific hairpin at the genome origin of replication. Introduces an endonucleolytic nick within the conserved sequence 5'-AGTATTAC-3' in the intergenic region of the genome, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities. ATPase activity is probably carried by the isoform Rep (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Cofactori

Mg2+Curated, Mn2+CuratedNote: Divalent metal cations, possibly Mg(2+) or Mn(2+).Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi70 – 701Divalent metal cationSequence Analysis
Metal bindingi78 – 781Divalent metal cationSequence Analysis
Active sitei116 – 1161For DNA cleavage activityBy similarity
Metal bindingi120 – 1201Divalent metal cationSequence Analysis

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi192 – 1998ATPBy similarity

GO - Molecular functioni

  1. ATPase activity, uncoupled Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. DNA binding Source: UniProtKB-KW
  4. endodeoxyribonuclease activity, producing 5'-phosphomonoesters Source: InterPro
  5. metal ion binding Source: UniProtKB-KW
  6. nucleotidyltransferase activity Source: UniProtKB-KW
  7. RNA binding Source: InterPro
  8. RNA helicase activity Source: InterPro

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
  2. protein-DNA covalent cross-linking Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Helicase, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replication-associated protein (EC:2.7.7.-, EC:3.1.21.-, EC:3.6.1.3)
Alternative name(s):
ATP-dependent helicase Rep
RepP
Gene namesi
Name:rep
OrganismiPigeon circovirus (PiCV) (Columbid circovirus)
Taxonomic identifieri126070 [NCBI]
Taxonomic lineageiVirusesssDNA virusesCircoviridaeCircovirusunclassified Circovirus
Virus hostiColumba livia (Domestic pigeon) [TaxID: 8932]
ProteomesiUP000000473: Genome

Subcellular locationi

Host nucleus Curated

GO - Cellular componenti

  1. host cell nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 317317Replication-associated proteinPRO_0000319865Add
BLAST

Keywords - PTMi

Covalent protein-DNA linkage

Interactioni

Subunit structurei

Interacts with the capsid protein; this interaction relocates Rep into the nucleus.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9IG45.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi5 – 4036Nuclear localization signalSequence AnalysisAdd
BLAST
Motifi41 – 444RCR-1By similarity
Motifi78 – 836RCR-2By similarity
Motifi87 – 10721Nuclear localization signalSequence AnalysisAdd
BLAST
Motifi116 – 1194RCR-3By similarity

Domaini

There are 3 rolling circle replication (RCR) motifs. RCR-2 is probably involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR.

Sequence similaritiesi

Family and domain databases

InterProiIPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR027417. P-loop_NTPase.
IPR003365. Viral_rep_N.
[Graphical view]
PfamiPF00910. RNA_helicase. 1 hit.
PF02407. Viral_Rep. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9IG45-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAPCKPGSNP PKGRVSAAEG GARREATRRP PREAAAKRWC FTLNNPTEEE
60 70 80 90 100
IKSLETWLVS DFHYAIVGKE VGEQGTPHLQ GFVHLKQKKR LPQLKQLFKR
110 120 130 140 150
AHWEKARGSD EDNEKYCSKE GNVLLTLGIP AKGNRSDLSE AVAAVKAGRA
160 170 180 190 200
MTEVARDFSE IYVKYGRGLR DLKLLIGQQP RDFKTEVIVI TGPPGCGKSR
210 220 230 240 250
WAADYPGSKF YKMKGEWWDG YDHQEVVIID DFYGWLPFCE LLRVTDRYPH
260 270 280 290 300
KVPVKGAFVE FTSRVIIVTS NSPPDAWYSE ERCCVQALFR RINKWLVWNH
310
DKFEDAPDCM KKYPINY
Length:317
Mass (Da):36,254
Last modified:October 1, 2000 - v1
Checksum:i988D75198E300EE6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF252610 Genomic DNA. Translation: AAF74196.1.
RefSeqiNP_059527.1. NC_002361.1.

Genome annotation databases

GeneIDi1460801.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF252610 Genomic DNA. Translation: AAF74196.1 .
RefSeqi NP_059527.1. NC_002361.1.

3D structure databases

ProteinModelPortali Q9IG45.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1460801.

Family and domain databases

InterProi IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR027417. P-loop_NTPase.
IPR003365. Viral_rep_N.
[Graphical view ]
Pfami PF00910. RNA_helicase. 1 hit.
PF02407. Viral_Rep. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning and sequencing of columbid circovirus (coCV), a new circovirus from pigeons."
    Mankertz A., Hattermann K., Ehlers B., Soike D.
    Arch. Virol. 145:2469-2479(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiREP_PICV
AccessioniPrimary (citable) accession number: Q9IG45
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: October 1, 2000
Last modified: November 26, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3