Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9IG45

- REP_PICV

UniProt

Q9IG45 - REP_PICV

Protein

Replication-associated protein

Gene

rep

Organism
Pigeon circovirus (PiCV) (Columbid circovirus)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 45 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Essential for the replication of viral ssDNA. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep and/or Rep' binds a specific hairpin at the genome origin of replication. Introduces an endonucleolytic nick within the conserved sequence 5'-AGTATTAC-3' in the intergenic region of the genome, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities. ATPase activity is probably carried by the isoform Rep By similarity.By similarity

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Cofactori

    Divalent metal cations, possibly magnesium or manganese.Curated

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi70 – 701Divalent metal cationSequence Analysis
    Metal bindingi78 – 781Divalent metal cationSequence Analysis
    Active sitei116 – 1161For DNA cleavage activityBy similarity
    Metal bindingi120 – 1201Divalent metal cationSequence Analysis

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi192 – 1998ATPBy similarity

    GO - Molecular functioni

    1. ATPase activity, uncoupled Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. DNA binding Source: UniProtKB-KW
    4. endodeoxyribonuclease activity, producing 5'-phosphomonoesters Source: InterPro
    5. metal ion binding Source: UniProtKB-KW
    6. nucleotidyltransferase activity Source: UniProtKB-KW
    7. RNA binding Source: InterPro
    8. RNA helicase activity Source: InterPro

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW
    2. protein-DNA covalent cross-linking Source: InterPro

    Keywords - Molecular functioni

    Endonuclease, Helicase, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    ATP-binding, DNA-binding, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Replication-associated protein (EC:2.7.7.-, EC:3.1.21.-, EC:3.6.1.3)
    Alternative name(s):
    ATP-dependent helicase Rep
    RepP
    Gene namesi
    Name:rep
    OrganismiPigeon circovirus (PiCV) (Columbid circovirus)
    Taxonomic identifieri126070 [NCBI]
    Taxonomic lineageiVirusesssDNA virusesCircoviridaeCircovirusunclassified Circovirus
    Virus hostiColumba livia (Domestic pigeon) [TaxID: 8932]
    ProteomesiUP000000473: Genome

    Subcellular locationi

    Host nucleus Curated

    GO - Cellular componenti

    1. host cell nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 317317Replication-associated proteinPRO_0000319865Add
    BLAST

    Keywords - PTMi

    Covalent protein-DNA linkage

    Interactioni

    Subunit structurei

    Interacts with the capsid protein; this interaction relocates Rep into the nucleus.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ9IG45.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi5 – 4036Nuclear localization signalSequence AnalysisAdd
    BLAST
    Motifi41 – 444RCR-1By similarity
    Motifi78 – 836RCR-2By similarity
    Motifi87 – 10721Nuclear localization signalSequence AnalysisAdd
    BLAST
    Motifi116 – 1194RCR-3By similarity

    Domaini

    There are 3 rolling circle replication (RCR) motifs. RCR-2 is probably involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR.

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR000605. Helicase_SF3_ssDNA/RNA_vir.
    IPR027417. P-loop_NTPase.
    IPR003365. Viral_rep_N.
    [Graphical view]
    PfamiPF00910. RNA_helicase. 1 hit.
    PF02407. Viral_Rep. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9IG45-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAPCKPGSNP PKGRVSAAEG GARREATRRP PREAAAKRWC FTLNNPTEEE    50
    IKSLETWLVS DFHYAIVGKE VGEQGTPHLQ GFVHLKQKKR LPQLKQLFKR 100
    AHWEKARGSD EDNEKYCSKE GNVLLTLGIP AKGNRSDLSE AVAAVKAGRA 150
    MTEVARDFSE IYVKYGRGLR DLKLLIGQQP RDFKTEVIVI TGPPGCGKSR 200
    WAADYPGSKF YKMKGEWWDG YDHQEVVIID DFYGWLPFCE LLRVTDRYPH 250
    KVPVKGAFVE FTSRVIIVTS NSPPDAWYSE ERCCVQALFR RINKWLVWNH 300
    DKFEDAPDCM KKYPINY 317
    Length:317
    Mass (Da):36,254
    Last modified:October 1, 2000 - v1
    Checksum:i988D75198E300EE6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF252610 Genomic DNA. Translation: AAF74196.1.
    RefSeqiNP_059527.1. NC_002361.1.

    Genome annotation databases

    GeneIDi1460801.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF252610 Genomic DNA. Translation: AAF74196.1 .
    RefSeqi NP_059527.1. NC_002361.1.

    3D structure databases

    ProteinModelPortali Q9IG45.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1460801.

    Family and domain databases

    InterProi IPR000605. Helicase_SF3_ssDNA/RNA_vir.
    IPR027417. P-loop_NTPase.
    IPR003365. Viral_rep_N.
    [Graphical view ]
    Pfami PF00910. RNA_helicase. 1 hit.
    PF02407. Viral_Rep. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of columbid circovirus (coCV), a new circovirus from pigeons."
      Mankertz A., Hattermann K., Ehlers B., Soike D.
      Arch. Virol. 145:2469-2479(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiREP_PICV
    AccessioniPrimary (citable) accession number: Q9IG45
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 26, 2008
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 45 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3