Q9IFX2 (NS1AB_HASV8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-structural polyprotein 1AB

Cleaved into the following 5 chains:

1. Protein p19
2. Transmembrane protein 1A
3. Serine protease p27
   Short name=p27
   EC=3.4.21.-
4. Protein p20
5. RNA-directed RNA polymerase p57
   Short name=p57
   EC=2.7.7.48

Gene names

Name:

ORF1

Organism

Human astrovirus-8 (HAstV-8) [Complete proteome]

Taxonomic identifier

43358 [NCBI]

Taxonomic lineage

Viruses › ssRNA positive-strand viruses, no DNA stage › Astroviridae › Mamastrovirus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	1417 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Non-structural polyprotein 1AB contains the viral protease participating in the cleavage of the polyprotein into functional products. It contains also the activities necessary for replication of genomic RNA, as well as transcription of subgenomic mRNA.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subcellular location	Host membrane; Multi-pass membrane protein Potential.
Post-translational modification	Cleaved presumably by viral and host proteases.
Sequence similarities	Belongs to the astroviridae polyprotein 1AB family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication
Cellular component	Host membrane Membrane
Coding sequence diversity	Ribosomal frameshifting
Domain	Coiled coil Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Nucleotidyltransferase Protease RNA-directed RNA polymerase Thiol protease Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro viral genome replication Inferred from electronic annotation. Source: InterPro
Cellular component	host cell membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro serine-type exopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by ribosomal frameshifting. [Align] [Select]

Isoform nsp1ab (identifier: Q9IFX2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Generated by a ribosomal frameshift at position 900.

Isoform nsp1a (identifier: Q9IFX3-1)

The sequence of this isoform can be found in the external entry Q9IFX3.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1417	1417	Non-structural polyprotein 1AB		PRO_0000327289
	Chain	1 – 176	176	Protein p19 Potential		PRO_0000327290
	Chain	177 – 420	244	Transmembrane protein 1A Potential		PRO_0000327291
	Chain	421 – 665	245	Serine protease p27 Potential		PRO_0000327292
	Chain	666 – 914	249	Protein p20 Potential		PRO_0000327293
	Chain	915 – 1417	503	RNA-directed RNA polymerase p57 Potential		PRO_0000327294
Regions
	Transmembrane	155 – 175	21	Helical; Potential
	Transmembrane	240 – 260	21	Helical; Potential
	Transmembrane	287 – 307	21	Helical; Potential
	Transmembrane	314 – 334	21	Helical; Potential
	Transmembrane	345 – 365	21	Helical; Potential
	Domain	1162 – 1288	127	RdRp catalytic
	Coiled coil	105 – 143	39	Potential
	Coiled coil	588 – 615	28	Potential
Sites
	Active site	462	1	Charge relay system; for serine protease activity By similarity
	Active site	490	1	Charge relay system; for serine protease activity By similarity
	Active site	552	1	Charge relay system; for serine protease activity By similarity
	Site	176 – 177	2	Cleavage Potential
	Site	420 – 421	2	Cleavage Potential
	Site	665 – 666	2	Cleavage Potential
	Site	914 – 915	2	Cleavage Potential

Sequences

	Sequence		Length	Mass (Da)
	Isoform nsp1ab [UniParc]. Last modified April 8, 2008. Version 2. Checksum: 94F6E43DBE61548B	FASTA	1,417	161,559
	10 20 30 40 50 60 MMALGEPYYS SKPDKDFNFG STMARRQMTP TMVTKLPKFV RNSPQAYDWI VRGLIFPTTG 70 80 90 100 110 120 KTYFQRVVVI TGGLEDGTYG SYAFNGSEWV EIYPIEHLNL MSSLKLIHKA NALQERLRLS 130 140 150 160 170 180 QEEKATLALD VQFLQHENVR LKELIPKPEP RKIQMKWIIV GAVLTFLSLI PGGYAQSQTN 190 200 210 220 230 240 NTIFTDMIAA CKYSTETLTE NLDLRIKLAL ANITINDKLD AVRQILNFAF VPRAHWLRTV 250 260 270 280 290 300 FYYIHYYEMW NIFMFVLAIG TVMRSARPGT DLITLATSHL SGFRMAVLPT IPFHTTMTLW 310 320 330 340 350 360 VMNTLMVCYY FDNLLAITMA ILAPILGIIF LCFMEDSNYV SQIRGLIATA VLIAGGHACL 370 380 390 400 410 420 TLTGTTTSLF VVILTCRFIR MATVFIGTRF EIRDANGKVV ATVPTRIKNV AFDFFQKLKQ 430 440 450 460 470 480 SGVRVGVNDF VVIKPGALCI IDTPEGKGTG FFSGNDIVTA AHVVGNNTFV SVCYEGLVYE 490 500 510 520 530 540 AKVRYMPEKD IAFITCPGDL HPTARLKLSK NPDYSCVTVM AYVNEDLVVS TATAMVHGNT 550 560 570 580 590 600 LSYAVRTQDG MSGAPVCDKY GRVLAVHQTN TGYTGGAVII DPADFHPVKA PSQVELLKEE 610 620 630 640 650 660 IERLKAQLNS AAENPVTVVT QQPIVTLEQK SVSDSDVVDL VRTAMEREMK VLRDEINGIL 670 680 690 700 710 720 APFLQKKKGK TKHGRGRVRR NLRKGVKLLT EEEYRELLEK GLDRETFLDL IDRIIGERSG 730 740 750 760 770 780 YPDYDDEDYY DEDDDGWGMV GDDVEFDYTE VINFDQAKPT PAPRTTKPKP CPEPKIEAQP 790 800 810 820 830 840 LDLSQKKEKQ PEHEQQVAKP TKPQKIEPQP YSQTYGKAPI WESYDFDWDE DDAKFILPAP 850 860 870 880 890 900 HRLTKADEIV LGSKIVKLRT IIETAIKTQN YSALPEAVFE LDKAAYEAGL EGFLQRVKSK 910 920 930 940 950 960 KQGPKKLQRA PEDQGAQNYH SLDAWKSLLE PPRERRCVPA NFPLLGHLPI NRPIFDDKKP 970 980 990 1000 1010 1020 RDDLLGLLPE PTWHAFEEYG PTTWGPQAFV KSFDKFFYAE PIDFFSEYPQ LCAFADWATY 1030 1040 1050 1060 1070 1080 REFRYLEDTR VIHITATEKN TDSTPAYPKM NYFDTEEDYL EAHGWAPYIR EFTRVFKGDK 1090 1100 1110 1120 1130 1140 PEVLWYLFLK KEIIKEEKIR NSDIRQIVCA DPIYTRIGAC LEAHQNALMK QHTDTSVGQC 1150 1160 1170 1180 1190 1200 GWSPMEGGFK KTMQRLVNKG NKHFIEFDWT RYDGTIPPAL FKHIKEIRWN FINKDQREKY 1210 1220 1230 1240 1250 1260 RHVHEWYVDN LLNRHVLLPS GEVTLQTRGN PSGQFSTPMD NNMVNFWLQA FEFAYFNGPD 1270 1280 1290 1300 1310 1320 KDLWKTYDTV VYGDDRLSTT PSVPDNYEER VITMYRDIFG MWVKPGKVIC RDSIVGLSFC 1330 1340 1350 1360 1370 1380 GFTVNENLEP VPTSPEKLMA SLLKPYKILP DLESLHGKLL CYQLLAAFMA EDHPFKVYVE 1390 1400 1410 HCLSRTAKQL RDSGLPARLT EEQLHRIWRG GPKKCDG
	Isoform nsp1a [UniParc]. See Q9IFX3.		–	–

References

[1]	"Molecular analysis of a serotype 8 human astrovirus genome." Mendez-Toss M., Romero-Guido P., Munguia M.E., Mendez E., Arias C.F. J. Gen. Virol. 81:2891-2897(2000) [PubMed: 11086120] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate Yuc-8.
[2]	"Protein products of the open reading frames encoding nonstructural proteins of human astrovirus serotype 8." Mendez E., Salas-Ocampo M.P., Munguia M.E., Arias C.F. J. Virol. 77:11378-11384(2003) [PubMed: 14557623] [Abstract] Cited for: NSP1AB POLYPROTEIN PROCESSING.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF260508 Genomic RNA. Translation: AAF85963.1. Sequence problems.
PIR	C49529.
3D structure databases
ProteinModelPortal	Q9IFX2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR022068. Astroviridae_polyprotein_1. IPR009003. Pept_cys/ser_Trypsin-like. IPR001730. Peptidase_C4. IPR001205. RNA-dir_pol_picornavirus. IPR007094. RNA-dir_pol_PSvirus. [Graphical view]
Pfam	PF12285. DUF3621. 1 hit. PF00863. Peptidase_C4. 1 hit. PF00680. RdRP_1. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50507. RDRP_SSRNA_POS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NS1AB_HASV8

Accession

Primary (citable) accession number: Q9IFX2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	April 8, 2008
Last modified:	November 16, 2011
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program