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Q9ICP7 (MREP_SCSVF) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Protein attributes

Sequence length286 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Essential for the replication of all genomic viral ssDNA (trans-replication). The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific hairpin at the genome origin of replication. Introduces an endonucleolytic nick within the conserved sequence 5'-A[GT]TATTAC-3' in the intergenic region of the genome, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities By similarity.

Catalytic activity

ATP + H2O = ADP + phosphate.

Cofactor

Divalent metal cations, possibly magnesium or manganese By similarity.

Subunit structure

Homooligomer Potential. Rep binds to repeated DNA motifs (iterons) By similarity.

Subcellular location

Host nucleus Potential.

Domain

There are 3 rolling circle replication (RCR) motifs. RCR-2 is probably involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR By similarity.

Miscellaneous

The genome of nanoviruses is composed of six to eight segments. In addition, some isolates contain subviral DNAs.

Sequence similarities

Belongs to the nanoviridea/circoviridae replication-associated protein family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 286286Master replication protein
PRO_0000378521

Regions

Nucleotide binding180 – 1889ATP By similarity
Motif10 – 134RCR-1 By similarity
Motif41 – 466RCR-2 By similarity
Motif50 – 7021Nuclear localization signal Potential
Motif79 – 824RCR-3 By similarity
Motif96 – 1027Nuclear localization signal Potential

Sites

Active site791For DNA cleavage activity By similarity
Metal binding331Divalent metal cation Potential
Metal binding411Divalent metal cation Potential
Metal binding841Divalent metal cation Potential

Sequences

Sequence LengthMass (Da)Tools
Q9ICP7 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 3E49185793F09C58

FASTA28633,281
        10         20         30         40         50         60 
MARQVICWCF TLNNPLAPLS LHESMKYLVY QTEAGDNGTI HYQGYVEMKK RTSLVQMKKL 

        70         80         90        100        110        120 
LPGAHLEKRR GSQGEARAYA MKEDSRVEGP WEFGEFKEVL EDKLRSVMED MKSTGKRPVE 

       130        140        150        160        170        180 
YIEDCCNTYD KSSATLREFR GELKKKQAIE EWQLQRQPWM DEVERLMETK DCRRIIWVYG 

       190        200        210        220        230        240 
PQGGEGKTSY AKHLVKTRDA FYSTGGKTAD IAFAWDHQEL VLFDFPRSFE EYVNYGVIEQ 

       250        260        270        280 
LKNGIVQSGK YQSIVKYCNY VEVIVFANFI PRSGMFSEDR IVIVYA 

« Hide

References

[1]"The master rep concept in nanovirus replication: identification of missing genome components and potential for natural genetic reassortment."
Timchenko T., Katul L., Sano Y., de Kouchkovsky F., Vetten H.J., Gronenborn B.
Virology 274:189-195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ290434 Genomic DNA. Translation: CAB96405.1.
RefSeqNP_620694.1. NC_003812.1.

3D structure databases

ProteinModelPortalQ9ICP7.
SMRQ9ICP7. Positions 2-95.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID991157.

Family and domain databases

InterProIPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR003365. Viral_rep_N.
[Graphical view]
PfamPF00910. RNA_helicase. 1 hit.
PF02407. Viral_Rep. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMREP_SCSVF
AccessionPrimary (citable) accession number: Q9ICP7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families