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Q9ICB2

- NSP3_ROTAM

UniProt

Q9ICB2 - NSP3_ROTAM

Protein

Non-structural protein 3

Gene
N/A
Organism
Rotavirus A (strain Human/United States/M/1976 G3-Px[x]-Ix-Rx-Cx-Mx-Ax-Nx-T1-E1-H1) (RV-A)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 38 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Involved in translation of viral mRNAs and shutoff of host protein synthesis. It is unclear whether it is required for the translation of viral mRNAs as well. Functions similarly to, and competes with the cellular poly(A)-binding protein PABPC1. Binds the conserved sequence 'GACC' at the 3' end of viral mRNAs and allows circularization of viral mRNAs in translation. Interacts with ZC3H7B/RoXaN and with the eukaryotic translation initiation factor eIF4G, using the same region employed by PABP-C1. NSP3 thus displaces PABPC1 from eIF4G, inhibiting the translation of cellular poly(A) mRNAs. Also responsible for the nuclear relocalization of PABPC1 upon rotavirus infection By similarity.By similarity

    GO - Molecular functioni

    1. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. regulation of translation Source: UniProtKB-KW
    2. suppression by virus of host gene expression Source: UniProtKB-KW

    Keywords - Biological processi

    Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Translation regulation

    Keywords - Ligandi

    RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Non-structural protein 3
    Short name:
    NSP3
    Alternative name(s):
    NCVP4
    Non-structural RNA-binding protein 34
    Short name:
    NS34
    OrganismiRotavirus A (strain Human/United States/M/1976 G3-Px[x]-Ix-Rx-Cx-Mx-Ax-Nx-T1-E1-H1) (RV-A)
    Taxonomic identifieri578834 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
    Virus hostiHomo sapiens (Human) [TaxID: 9606]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 310310Non-structural protein 3PRO_0000369456Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer. Interacts (via the coiled-coil region) with host ZC3H7B (via LD motif). Interacts with host EIF4G1 By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ9ICB2.
    SMRiQ9ICB2. Positions 4-160, 203-309.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 146146RNA-bindingBy similarityAdd
    BLAST
    Regioni147 – 20357DimerizationBy similarityAdd
    BLAST
    Regioni167 – 23165Interaction with ZC3H7BBy similarityAdd
    BLAST
    Regioni205 – 310106Interaction with EIF4G1By similarityAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili163 – 23472Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the rotavirus A NSP3 family.Curated

    Keywords - Domaini

    Coiled coil

    Family and domain databases

    InterProiIPR002873. Rotavirus_NSP3.
    [Graphical view]
    PfamiPF01665. Rota_NSP3. 1 hit.
    [Graphical view]
    SUPFAMiSSF69903. SSF69903. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9ICB2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MESTQQMVSS IINTSFEAAV VAATSTLELM GIQYDYNEVF TRVKSKFDYV    50
    MDDSGVKNNL LGKAITIDQA LNGKFGSAIR NRNWMTDSKT VAKLDEDVNK 100
    LRMTLSSKGI DQKMRVLNAC FSVKRIPGKS SSIIKCTRLM KDKIERGEVE 150
    VDDSYVDEKM EIDTIDWKSR YDQLEKRFES LKQRVNEKYN TWVQKAKKVN 200
    ENMYSLQNVI SQQQNQIADL QQYCNKLEAD LQGKFSSLVS SVEWYLRSME 250
    LPDDVKNDIE QQLNSIDLIN PINAIDDIES LIRNLIQDYD RTFLMLKGLL 300
    KQCNYEYAYE 310
    Length:310
    Mass (Da):35,863
    Last modified:October 1, 2000 - v1
    Checksum:iF756D9DDB36A61FE
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti310 – 3101E → D.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF190171 Genomic RNA. Translation: AAF81407.1.
    AF190172 Genomic RNA. Translation: AAF81408.1.
    AF338246 Genomic RNA. Translation: AAK74116.1.
    AF338247 Genomic RNA. Translation: AAK74117.1.
    AF338247 Genomic RNA. Translation: AAK74118.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF190171 Genomic RNA. Translation: AAF81407.1 .
    AF190172 Genomic RNA. Translation: AAF81408.1 .
    AF338246 Genomic RNA. Translation: AAK74116.1 .
    AF338247 Genomic RNA. Translation: AAK74117.1 .
    AF338247 Genomic RNA. Translation: AAK74118.1 .

    3D structure databases

    ProteinModelPortali Q9ICB2.
    SMRi Q9ICB2. Positions 4-160, 203-309.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR002873. Rotavirus_NSP3.
    [Graphical view ]
    Pfami PF01665. Rota_NSP3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF69903. SSF69903. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Rearrangement generated in double genes, NSP1 and NSP3, of viable progenies from a human rotavirus strain."
      Kojima K., Taniguchi K., Kawagishi-Kobayashi M., Matsuno S., Urasawa S.
      Virus Res. 67:163-171(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: IGV-F and IGV-S.
    2. "A human rotavirus with rearranged genes 7 and 11 encodes a modified NSP3 protein and suggests an additional mechanism for gene rearrangement."
      Gault E., Schnepf N., Poncet D., Servant A., Teran S., Garbarg-Chenon A.
      J. Virol. 75:7305-7314(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiNSP3_ROTAM
    AccessioniPrimary (citable) accession number: Q9ICB2
    Secondary accession number(s): Q91ME7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 38 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3