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Q9ICB2

- NSP3_ROTAM

UniProt

Q9ICB2 - NSP3_ROTAM

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Protein
Non-structural protein 3
Gene
N/A
Organism
Rotavirus A (strain Human/United States/M/1976 G3-Px[x]-Ix-Rx-Cx-Mx-Ax-Nx-T1-E1-H1) (RV-A)
Status
Reviewed - Annotation score: 2 out of 5 - Protein inferred from homologyi

Functioni

Involved in translation of viral mRNAs and shutoff of host protein synthesis. It is unclear whether it is required for the translation of viral mRNAs as well. Functions similarly to, and competes with the cellular poly(A)-binding protein PABPC1. Binds the conserved sequence 'GACC' at the 3' end of viral mRNAs and allows circularization of viral mRNAs in translation. Interacts with ZC3H7B/RoXaN and with the eukaryotic translation initiation factor eIF4G, using the same region employed by PABP-C1. NSP3 thus displaces PABPC1 from eIF4G, inhibiting the translation of cellular poly(A) mRNAs. Also responsible for the nuclear relocalization of PABPC1 upon rotavirus infection By similarity.

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. suppression by virus of host translation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Translation regulation

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 3
Short name:
NSP3
Alternative name(s):
NCVP4
Non-structural RNA-binding protein 34
Short name:
NS34
OrganismiRotavirus A (strain Human/United States/M/1976 G3-Px[x]-Ix-Rx-Cx-Mx-Ax-Nx-T1-E1-H1) (RV-A)
Taxonomic identifieri578834 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiHomo sapiens (Human) [TaxID: 9606]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 310310Non-structural protein 3
PRO_0000369456Add
BLAST

Interactioni

Subunit structurei

Homodimer. Interacts (via the coiled-coil region) with host ZC3H7B (via LD motif). Interacts with host EIF4G1 By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ9ICB2.
SMRiQ9ICB2. Positions 4-160, 203-309.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 146146RNA-binding By similarity
Add
BLAST
Regioni147 – 20357Dimerization By similarity
Add
BLAST
Regioni167 – 23165Interaction with ZC3H7B By similarity
Add
BLAST
Regioni205 – 310106Interaction with EIF4G1 By similarity
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili163 – 23472 Reviewed prediction
Add
BLAST

Sequence similaritiesi

Belongs to the rotavirus A NSP3 family.

Keywords - Domaini

Coiled coil

Family and domain databases

InterProiIPR002873. Rotavirus_NSP3.
[Graphical view]
PfamiPF01665. Rota_NSP3. 1 hit.
[Graphical view]
SUPFAMiSSF69903. SSF69903. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9ICB2-1 [UniParc]FASTAAdd to Basket

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MESTQQMVSS IINTSFEAAV VAATSTLELM GIQYDYNEVF TRVKSKFDYV    50
MDDSGVKNNL LGKAITIDQA LNGKFGSAIR NRNWMTDSKT VAKLDEDVNK 100
LRMTLSSKGI DQKMRVLNAC FSVKRIPGKS SSIIKCTRLM KDKIERGEVE 150
VDDSYVDEKM EIDTIDWKSR YDQLEKRFES LKQRVNEKYN TWVQKAKKVN 200
ENMYSLQNVI SQQQNQIADL QQYCNKLEAD LQGKFSSLVS SVEWYLRSME 250
LPDDVKNDIE QQLNSIDLIN PINAIDDIES LIRNLIQDYD RTFLMLKGLL 300
KQCNYEYAYE 310
Length:310
Mass (Da):35,863
Last modified:October 1, 2000 - v1
Checksum:iF756D9DDB36A61FE
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti310 – 3101E → D.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF190171 Genomic RNA. Translation: AAF81407.1.
AF190172 Genomic RNA. Translation: AAF81408.1.
AF338246 Genomic RNA. Translation: AAK74116.1.
AF338247 Genomic RNA. Translation: AAK74117.1.
AF338247 Genomic RNA. Translation: AAK74118.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF190171 Genomic RNA. Translation: AAF81407.1 .
AF190172 Genomic RNA. Translation: AAF81408.1 .
AF338246 Genomic RNA. Translation: AAK74116.1 .
AF338247 Genomic RNA. Translation: AAK74117.1 .
AF338247 Genomic RNA. Translation: AAK74118.1 .

3D structure databases

ProteinModelPortali Q9ICB2.
SMRi Q9ICB2. Positions 4-160, 203-309.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR002873. Rotavirus_NSP3.
[Graphical view ]
Pfami PF01665. Rota_NSP3. 1 hit.
[Graphical view ]
SUPFAMi SSF69903. SSF69903. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Rearrangement generated in double genes, NSP1 and NSP3, of viable progenies from a human rotavirus strain."
    Kojima K., Taniguchi K., Kawagishi-Kobayashi M., Matsuno S., Urasawa S.
    Virus Res. 67:163-171(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: IGV-F and IGV-S.
  2. "A human rotavirus with rearranged genes 7 and 11 encodes a modified NSP3 protein and suggests an additional mechanism for gene rearrangement."
    Gault E., Schnepf N., Poncet D., Servant A., Teran S., Garbarg-Chenon A.
    J. Virol. 75:7305-7314(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiNSP3_ROTAM
AccessioniPrimary (citable) accession number: Q9ICB2
Secondary accession number(s): Q91ME7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: October 1, 2000
Last modified: July 9, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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