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Protein

Zinc metalloproteinase/disintegrin

Gene
N/A
Organism
Deinagkistrodon acutus (Hundred-pace snake) (Agkistrodon acutus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Snake venom metalloproteinase MD2: impairs hemostasis in the envenomed animal.By similarity
Disintegrin: inhibits platelet aggregation induced by ADP, thrombin, platelet-activating factor and collagen. Acts by inhibiting fibrinogen interaction with platelet receptors GPIIb/GPIIIa (ITGA2B/ITGB3) (By similarity).By similarity

Miscellaneous

The disintegrin belongs to the medium disintegrin subfamily.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi183CalciumBy similarity1
Metal bindingi267CalciumBy similarity1
Metal bindingi316Zinc; catalyticBy similarity1
Active sitei317PROSITE-ProRule annotation1
Metal bindingi320Zinc; catalyticBy similarity1
Metal bindingi326Zinc; catalyticBy similarity1
Metal bindingi372Calcium; via carbonyl oxygenBy similarity1
Metal bindingi375CalciumBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionCell adhesion impairing toxin, Hemostasis impairing toxin, Hydrolase, Metalloprotease, Platelet aggregation inhibiting toxin, Protease, Toxin
LigandCalcium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc metalloproteinase/disintegrin
Cleaved into the following 2 chains:
Alternative name(s):
DaMD2
OrganismiDeinagkistrodon acutus (Hundred-pace snake) (Agkistrodon acutus)
Taxonomic identifieri36307 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeDeinagkistrodon

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei‹1 – 6Sequence analysis›6
PropeptideiPRO_00003226257 – 174By similarityAdd BLAST168
ChainiPRO_0000322626175 – 376Snake venom metalloproteinase MD2Add BLAST202
PropeptideiPRO_0000322627377 – 401By similarityAdd BLAST25
ChainiPRO_0000322628402 – 466DisintegrinAdd BLAST65

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi291 ↔ 372By similarity
Disulfide bondi331 ↔ 356By similarity
Disulfide bondi333 ↔ 339By similarity
Disulfide bondi422 ↔ 428By similarity
Disulfide bondi427 ↔ 452By similarity
Disulfide bondi440 ↔ 459By similarity

Keywords - PTMi

Disulfide bond, Zymogen

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9IAX6.
SMRiQ9IAX6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini180 – 377Peptidase M12BPROSITE-ProRule annotationAdd BLAST198
Domaini385 – 466DisintegrinPROSITE-ProRule annotationAdd BLAST82

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi444 – 446Cell attachment site3

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG006978.

Family and domain databases

CDDicd04269. ZnMc_adamalysin_II_like. 1 hit.
Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiView protein in InterPro
IPR001762. Disintegrin_dom.
IPR036436. Disintegrin_dom_sf.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
IPR034027. Reprolysin_adamalysin.
PfamiView protein in Pfam
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
PRINTSiPR00289. DISINTEGRIN.
SMARTiView protein in SMART
SM00050. DISIN. 1 hit.
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiView protein in PROSITE
PS50215. ADAM_MEPRO. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9IAX6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
FPYQGSSIIL ESGNVNDYEV VYPRKVTALP KGAVQQKYED TMQYEFKVNG
60 70 80 90 100
EPVVLHLEKN KGLFSKDYSE THYSPDGRRI TTHPLVEDHC YYRGRIRNDA
110 120 130 140 150
DSTASISACN GLKGHFKLRG ETYLIEPMKI SNSEAHAVYK YENVEKEDEA
160 170 180 190 200
HKMCGVTQNW ESYEPIKKAS QLIVTPEHQR YMEIVIVVDH SMYTKYNGDS
210 220 230 240 250
DKIKTWIYEM SNTIRESYRY LYIDTSVAAI EIWSEKDLIN VETSAKNTLE
260 270 280 290 300
SFGEWRARDL IHRISHDNAQ LLTATDLDGP TIGLAYVASM CDPKRSVGVV
310 320 330 340 350
QDHSSVNHLV AITLAHEIAH NLGVRHDEGS CSCGSGYTCI MSPVINPDAM
360 370 380 390 400
KYFSDCSYIQ CWDYIMKENP PCILNKPLRT DTVSTPVSGN ELLEAGKDYD
410 420 430 440 450
RDSSANPCYD AATCKLNQGA QCTAGPCCDQ GRFKEEGTIC RRARGDDLDD
460
YCNGISGDCP RNPYHA
Length:466
Mass (Da):52,518
Last modified:October 1, 2000 - v1
Checksum:iC582C9147BA3E92B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF117637 mRNA. Translation: AAF61189.1.

Similar proteinsi

Entry informationi

Entry nameiVM2M2_DEIAC
AccessioniPrimary (citable) accession number: Q9IAX6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: October 1, 2000
Last modified: October 25, 2017
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families