Q9IAX2 (IOD2_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type II iodothyronine deiodinase
EC=1.97.1.10

Alternative name(s):
5DII
DIOII
Type 2 DI
Type-II 5'-deiodinase

Gene names

Name:

DIO2

Organism

Gallus gallus (Chicken) [Reference proteome]

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	279 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). Essential for providing the brain with appropriate levels of T3 during the critical period of development. Ref.1 UniProtKB Q92813
Catalytic activity	3,5,3'-triiodo-L-thyronine + iodide + A + H+ = L-thyroxine + AH2.
Enzyme regulation	Not inhibited by N(6)-propylthiouracil.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Tissue specificity	Highly expressed in liver and in various parts of the brain including telencephalon, hippocampus, cerebellum, and brain stem, and weakly expressed in thyroid, lung, and small intestine. Not detected in skeletal muscle, heart atria or ventricle, gizzard or kidney. Ref.1
Sequence similarities	Belongs to the iodothyronine deiodinase family.
Biophysicochemical properties	Kinetic parameters: KM=1.1 nM for thyroxine Ref.1 Vmax=0.34 pmol/min/mg enzyme

Ontologies

Keywords
Biological process	Thyroid hormones biosynthesis
Cellular component	Membrane
Coding sequence diversity	Selenocysteine
Domain	Transmembrane Transmembrane helix
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	hormone biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW thyroid hormone generation Inferred from direct assay PubMed 12270789. Source: UniProtKB
Cellular_component	integral to membrane Non-traceable author statement. Source: UniProtKB
Molecular_function	selenium binding Non-traceable author statement. Source: UniProtKB thyroxine 5'-deiodinase activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 279	279	Type II iodothyronine deiodinase		PRO_0000154320
Regions
	Transmembrane	8 – 28	21	Helical; Potential
Sites
	Active site	132	1
Amino acid modifications
	Non-standard residue	132	1	Selenocysteine
	Non-standard residue	265	1	Selenocysteine

Sequences

	Sequence		Length	Mass (Da)
	Q9IAX2 [UniParc]. Last modified February 26, 2008. Version 3. Checksum: BC249728E2A8E9C1	FASTA	279	31,033
	10 20 30 40 50 60 MGLLSADLLI TLQILPVFFS NCLFLALYDS VILLKHMVLF LSRSKSARGE WRRMLTSEGL 70 80 90 100 110 120 RCVWNSFLLD AYKQVKLGGE APNSSVIHIA KGNDGSNSSW KSVGGKCGTK CHLLDFANSE 130 140 150 160 170 180 RPLVVNFGSA TUPPFTSQLS AFSKLVEEFS GVADFLLVYI DEAHPSDGWA APGISPSSFE 190 200 210 220 230 240 VKKHRNQEDR CAAAHQLLER FSLPPQCQVV ADCMDNNANV AYGVSFERVC IVQRQKIAYL 250 260 270 GGKGPFFYNL QEVRLWLEQN FSKRUNPLST EDLSTDVSL

References

[1]	"Cloning and expression of the chicken type 2 iodothyronine 5'-deiodinase." Gereben B., Bartha T., Tu H.M., Harney J.W., Rudas P., Larsen P.R. J. Biol. Chem. 274:13768-13776(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY. Tissue: Embryo and Pituitary.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF125575 mRNA. Translation: AAD33251.1.
IPI	IPI00592673.
UniGene	Gga.1819. Gga.51485.
3D structure databases
ProteinModelPortal	Q9IAX2.
ModBase	Search...
Proteomic databases
PRIDE	Q9IAX2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG000099.
Family and domain databases
InterPro	IPR000643. Iodothyronine_deiodinase. IPR008261. Iodothyronine_deiodinase_AS. [Graphical view]
PANTHER	PTHR11781. PTHR11781. 1 hit.
Pfam	PF00837. T4_deiodinase. 1 hit. [Graphical view]
PIRSF	PIRSF001330. IOD. 1 hit.
PROSITE	PS01205. T4_DEIODINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IOD2_CHICK

Accession

Primary (citable) accession number: Q9IAX2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	February 26, 2008
Last modified:	April 3, 2013
This is version 67 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program