Q9IAV3 (HHEX_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hematopoietically-expressed homeobox protein hhex
Short name=Homeobox protein hex

Gene names

Name:	hhex
Synonyms:	hex

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	228 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Recognizes the DNA sequence 5'-ATTAA-3' By similarity. Transcriptional repressor. Regulates the differentiation of both endothelial and blood cells. Plays a role in embryonic dorsoventral patterning by regulating bmp expression. May establish anterior identity. Functions in the embryo to regulate liver development. Functions extraembryonically to generate organ chirality. Ref.1 Ref.2 Ref.3 Ref.4 UniProtKB Q05502
Subcellular location	Nucleus Probable.
Tissue specificity	Expressed in embryonic endothelial and blood lineages. From late-blastula stage, expression is restricted to the dorsal marginal region of the extraembryonic yolk syncytial layer (YSL). By the onset of gastrulation, expressed in the entire dorsal half of the YSL. Post-gastrulation, expression appears in both anterior and posterior lateral plate mesoderm by the 3-somite stage. Posteriorly, expression is in the intermediate cell mass (ICM), which contains both endothelial and blood precursors. Subsequently expressed in the developing endothelial cells including the endocardium until the onset of circulation (24 hpf) and disappears completely by 30 hpf, at which point expression is seen in the thyroid and liver primordia. Also expressed in the developing biliary tree and pancreas. Ref.1 Ref.2 Ref.4
Induction	By dhama/boz downstream of canonical wnt-signaling. Ref.5
Sequence similarities	Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Homeobox
Ligand	DNA-binding
Molecular function	Developmental protein Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	anterior/posterior pattern specification Inferred from sequence or structural similarity. Source: UniProtKB determination of left/right symmetry Inferred from mutant phenotype Ref.3. Source: UniProtKB digestive tract development Inferred from mutant phenotype Ref.3. Source: UniProtKB dorsal/ventral pattern formation Inferred from mutant phenotype Ref.1. Source: UniProtKB embryonic hemopoiesis Inferred from mutant phenotype Ref.2. Source: UniProtKB heart development Inferred from mutant phenotype Ref.4. Source: ZFIN liver development Inferred from mutant phenotype Ref.3. Source: UniProtKB negative regulation of transcription from RNA polymerase II promoter Inferred from direct assay Ref.1. Source: UniProtKB positive regulation of endothelial cell differentiation Inferred from mutant phenotype Ref.2. Source: ZFIN regulation of organ growth Inferred from mutant phenotype Ref.4. Source: ZFIN thyroid gland development Inferred from mutant phenotype Ref.4. Source: ZFIN transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	sequence-specific DNA binding Inferred from sequence or structural similarity. Source: UniProtKB sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 228	228	Hematopoietically-expressed homeobox protein hhex		PRO_0000326219
Regions
	DNA binding	117 – 176	60	Homeobox

Sequences

	Sequence		Length	Mass (Da)
	Q9IAV3 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 9F44C99F9772898B	FASTA	228	26,048
	10 20 30 40 50 60 MQFQHPHAPL YAPAPAPPAH PTPFYIEDIL GRTGSSSGPV VPTPTLPSPN SSFTSLIPSY 70 80 90 100 110 120 RTPIYELTPI HPVLSQYASM YPFQRSVGDF AHALIRHDPL GKPLLWSPFI QRPLHKRKGG 130 140 150 160 170 180 QVRFSNDQTI ELEKKFETQK YLSPPERKRL AKMLQLSERQ VKTWFQNRRA KWRRLKQENP 190 200 210 220 PSTGKREAED SDTRRLSDAA ARARELESGA STDSEELLDI EDEHQFTL

References

[1]	"A role for the extraembryonic yolk syncytial layer in patterning the zebrafish embryo suggested by properties of the hex gene." Ho C.-Y., Houart C., Wilson S.W., Stainier D.Y.R. Curr. Biol. 9:1131-1134(1999) [PubMed: 10531010] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Tissue: Pharyngula.
[2]	"Hhex and scl function in parallel to regulate early endothelial and blood differentiation in zebrafish." Liao W., Ho C.-Y., Yan Y.L., Postlethwait J.H., Stainier D.Y.R. Development 127:4303-4313(2000) [PubMed: 11003831] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[3]	"Zebrafish hhex regulates liver development and digestive organ chirality." Wallace K.N., Yusuff S., Sonntag J.M., Chin A.J., Pack M. Genesis 30:141-143(2001) [PubMed: 11477693] [Abstract] Cited for: FUNCTION.
[4]	"Zebrafish hhex, nk2.1a, and pax2.1 regulate thyroid growth and differentiation downstream of Nodal-dependent transcription factors." Elsalini O.A., von Gartzen J., Cramer M., Rohr K.B. Dev. Biol. 263:67-80(2003) [PubMed: 14568547] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[5]	"Regulation of hhex expression in the yolk syncytial layer, the potential Nieuwkoop center homolog in zebrafish." Bischof J., Driever W. Dev. Biol. 276:552-562(2004) [PubMed: 15581885] [Abstract] Cited for: INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF131070 mRNA. Translation: AAF28383.1.
IPI	IPI00485854.
RefSeq	NP_571009.1. NM_130934.1.
UniGene	Dr.79053.
3D structure databases
HSSP	HSSP built from PDB template 1NK3 based on UniProtKB P22808.
ProteinModelPortal	Q9IAV3.
SMR	Q9IAV3. Positions 121-174.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9IAV3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	30098.
KEGG	dre:30098.
Organism-specific databases
CTD	3087.
ZFIN	ZDB-GENE-980526-299. hhex.
Phylogenomic databases
HOVERGEN	HBG055460.
OrthoDB	EOG4XKV7W.
Gene expression databases
ArrayExpress	Q9IAV3.
Family and domain databases
InterPro	IPR001356. Homeobox. IPR017970. Homeobox_CS. IPR020479. Homeobox_eu. IPR009057. Homeodomain-like. IPR012287. Homeodomain-rel. [Graphical view]
Gene3D	G3DSA:1.10.10.60. Homeodomain-rel. 1 hit.
KO	K08024.
Pfam	PF00046. Homeobox. 1 hit. [Graphical view]
PRINTS	PR00024. HOMEOBOX.
SMART	SM00389. HOX. 1 hit. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 1 hit.
PROSITE	PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HHEX_DANRE

Accession

Primary (citable) accession number: Q9IAV3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	October 1, 2000
Last modified:	December 14, 2011
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program