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Protein

Basic phospholipase A2 homolog BnSP-7

Gene
N/A
Organism
Bothrops pauloensis (Neuwied's lancehead) (Bothrops neuwiedi pauloensis)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Snake venom phospholipase A2 (PLA2) that lacks enzymatic activity. Is myotoxic. Displays bactericidal activity and promotes the blockage of the neuromuscular contraction of the chick biventer cervicis muscle. Also disrupts artificial membranes, and provokes tissue damages characterized by edema, necrosis and inflammation. May act as pro-inflammatory mediators, inducing metalloproteinase and cytokine production from the inflammatory and satellite cells.3 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Myotoxin, Neurotoxin, Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Basic phospholipase A2 homolog BnSP-7
Short name:
svPLA2 homolog
Alternative name(s):
Phospholipase A2 II
OrganismiBothrops pauloensis (Neuwied's lancehead) (Bothrops neuwiedi pauloensis)
Taxonomic identifieri1042543 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeBothrops

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

LD50 is 7.8 mg/kg by intraperitoneal injection into mice.1 Publication

Chemistry databases

ChEMBLiCHEMBL2146345.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001616251 – 120Basic phospholipase A2 homolog BnSP-7Add BLAST120

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi25 ↔ 1141 Publication
Disulfide bondi27 ↔ 431 Publication
Disulfide bondi42 ↔ 941 Publication
Disulfide bondi48 ↔ 1201 Publication
Disulfide bondi49 ↔ 871 Publication
Disulfide bondi56 ↔ 801 Publication
Disulfide bondi74 ↔ 851 Publication

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

Secondary structure

1120
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 12Combined sources11
Helixi16 – 20Combined sources5
Turni24 – 26Combined sources3
Beta strandi27 – 30Combined sources4
Helixi38 – 50Combined sources13
Turni58 – 60Combined sources3
Beta strandi65 – 68Combined sources4
Beta strandi71 – 74Combined sources4
Helixi79 – 97Combined sources19
Helixi99 – 101Combined sources3
Helixi104 – 106Combined sources3
Helixi111 – 113Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PA0X-ray2.20A/B2-110[»]
1PC9X-ray2.50A/B2-120[»]
3MLMX-ray2.21A/B1-120[»]
SMRiQ9IAT9.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9IAT9.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

CDDicd00125. PLA2c. 1 hit.
Gene3Di1.20.90.10. 1 hit.
InterProiIPR001211. PLipase_A2.
IPR033112. PLipase_A2_Asp_AS.
IPR016090. PLipase_A2_dom.
IPR033113. PLipase_A2_His_AS.
[Graphical view]
PANTHERiPTHR11716. PTHR11716. 1 hit.
PfamiPF00068. Phospholip_A2_1. 1 hit.
[Graphical view]
PRINTSiPR00389. PHPHLIPASEA2.
SMARTiSM00085. PA2c. 1 hit.
[Graphical view]
SUPFAMiSSF48619. SSF48619. 1 hit.
PROSITEiPS00119. PA2_ASP. 1 hit.
PS00118. PA2_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9IAT9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
SFELGKMILQ ETGKNPAKSY GAYGCNCGVL GRGQPKDATD RCCYVHKCCY
60 70 80 90 100
KKLTGCDPKK DRYSYSWKDK TIVCGENNPC LKELCECDKA VAICLRENLG
110 120
TYNKKYRYHL KPFCKKADPC
Length:120
Mass (Da):13,614
Last modified:June 16, 2009 - v2
Checksum:iD6DF3C42CC26CAF5
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti34Q → G in BnSP-6. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF145781 mRNA. Translation: AAF66703.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF145781 mRNA. Translation: AAF66703.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PA0X-ray2.20A/B2-110[»]
1PC9X-ray2.50A/B2-120[»]
3MLMX-ray2.21A/B1-120[»]
SMRiQ9IAT9.
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

ChEMBLiCHEMBL2146345.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiQ9IAT9.

Family and domain databases

CDDicd00125. PLA2c. 1 hit.
Gene3Di1.20.90.10. 1 hit.
InterProiIPR001211. PLipase_A2.
IPR033112. PLipase_A2_Asp_AS.
IPR016090. PLipase_A2_dom.
IPR033113. PLipase_A2_His_AS.
[Graphical view]
PANTHERiPTHR11716. PTHR11716. 1 hit.
PfamiPF00068. Phospholip_A2_1. 1 hit.
[Graphical view]
PRINTSiPR00389. PHPHLIPASEA2.
SMARTiSM00085. PA2c. 1 hit.
[Graphical view]
SUPFAMiSSF48619. SSF48619. 1 hit.
PROSITEiPS00119. PA2_ASP. 1 hit.
PS00118. PA2_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPA2H_BOTPA
AccessioniPrimary (citable) accession number: Q9IAT9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: June 16, 2009
Last modified: November 30, 2016
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Caution

Does not bind calcium as one of the calcium-binding ligands is lost (Asp->Lys in position 46, which corresponds to 'Lys-49' in the current nomenclature).Curated

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.