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Protein

Basic phospholipase A2 homolog BnSP-7

Gene
N/A
Organism
Bothrops pauloensis (Neuwied's lancehead) (Bothrops neuwiedi pauloensis)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Snake venom phospholipase A2 (PLA2) that lacks enzymatic activity. Is myotoxic. Displays bactericidal activity and promotes the blockage of the neuromuscular contraction of the chick biventer cervicis muscle. Also disrupts artificial membranes, and provokes tissue damages characterized by edema, necrosis and inflammation. May act as pro-inflammatory mediators, inducing metalloproteinase and cytokine production from the inflammatory and satellite cells.3 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Myotoxin, Neurotoxin, Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Basic phospholipase A2 homolog BnSP-7
Short name:
svPLA2 homolog
Alternative name(s):
Phospholipase A2 II
OrganismiBothrops pauloensis (Neuwied's lancehead) (Bothrops neuwiedi pauloensis)
Taxonomic identifieri1042543 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeBothrops

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

LD50 is 7.8 mg/kg by intraperitoneal injection into mice.1 Publication

Chemistry

ChEMBLiCHEMBL2146345.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 120120Basic phospholipase A2 homolog BnSP-7PRO_0000161625Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi25 ↔ 1141 Publication
Disulfide bondi27 ↔ 431 Publication
Disulfide bondi42 ↔ 941 Publication
Disulfide bondi48 ↔ 1201 Publication
Disulfide bondi49 ↔ 871 Publication
Disulfide bondi56 ↔ 801 Publication
Disulfide bondi74 ↔ 851 Publication

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

Secondary structure

1
120
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 1211Combined sources
Helixi16 – 205Combined sources
Turni24 – 263Combined sources
Beta strandi27 – 304Combined sources
Helixi38 – 5013Combined sources
Turni58 – 603Combined sources
Beta strandi65 – 684Combined sources
Beta strandi71 – 744Combined sources
Helixi79 – 9719Combined sources
Helixi99 – 1013Combined sources
Helixi104 – 1063Combined sources
Helixi111 – 1133Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PA0X-ray2.20A/B2-110[»]
1PC9X-ray2.50A/B2-120[»]
3MLMX-ray2.21A/B1-120[»]
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9IAT9.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di1.20.90.10. 1 hit.
InterProiIPR001211. PLipase_A2.
IPR033112. PLipase_A2_Asp_AS.
IPR016090. PLipase_A2_dom.
IPR033113. PLipase_A2_His_AS.
[Graphical view]
PANTHERiPTHR11716. PTHR11716. 1 hit.
PfamiPF00068. Phospholip_A2_1. 1 hit.
[Graphical view]
PRINTSiPR00389. PHPHLIPASEA2.
SMARTiSM00085. PA2c. 1 hit.
[Graphical view]
SUPFAMiSSF48619. SSF48619. 1 hit.
PROSITEiPS00119. PA2_ASP. 1 hit.
PS00118. PA2_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9IAT9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
SFELGKMILQ ETGKNPAKSY GAYGCNCGVL GRGQPKDATD RCCYVHKCCY
60 70 80 90 100
KKLTGCDPKK DRYSYSWKDK TIVCGENNPC LKELCECDKA VAICLRENLG
110 120
TYNKKYRYHL KPFCKKADPC
Length:120
Mass (Da):13,614
Last modified:June 16, 2009 - v2
Checksum:iD6DF3C42CC26CAF5
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti34 – 341Q → G in BnSP-6. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF145781 mRNA. Translation: AAF66703.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF145781 mRNA. Translation: AAF66703.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PA0X-ray2.20A/B2-110[»]
1PC9X-ray2.50A/B2-120[»]
3MLMX-ray2.21A/B1-120[»]
ModBaseiSearch...
MobiDBiSearch...

Chemistry

ChEMBLiCHEMBL2146345.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiQ9IAT9.

Family and domain databases

Gene3Di1.20.90.10. 1 hit.
InterProiIPR001211. PLipase_A2.
IPR033112. PLipase_A2_Asp_AS.
IPR016090. PLipase_A2_dom.
IPR033113. PLipase_A2_His_AS.
[Graphical view]
PANTHERiPTHR11716. PTHR11716. 1 hit.
PfamiPF00068. Phospholip_A2_1. 1 hit.
[Graphical view]
PRINTSiPR00389. PHPHLIPASEA2.
SMARTiSM00085. PA2c. 1 hit.
[Graphical view]
SUPFAMiSSF48619. SSF48619. 1 hit.
PROSITEiPS00119. PA2_ASP. 1 hit.
PS00118. PA2_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Structural and functional characterization of BnSP-7, a Lys49 myotoxic phospholipase A(2) homologue from Bothrops neuwiedi pauloensis venom."
    Soares A.M., Guerra-Sa R., Borja-Oliveira C.R., Rodrigues V.M., Rodrigues-Simioni L., Rodrigues V., Fontes M.R.M., Lomonte B., Gutierrez J.M., Giglio J.R.
    Arch. Biochem. Biophys. 378:201-209(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-120, PROTEIN SEQUENCE OF 1-39, FUNCTION, LETHAL DOSE.
    Tissue: Venom gland.
  2. "Combined snake venomics and venom gland transcriptomic analysis of Bothropoides pauloensis."
    Rodrigues R.S., Boldrini-Franca J., Fonseca F.P., de la Torre P., Henrique-Silva F., Sanz L., Calvete J.J., Rodrigues V.M.
    J. Proteomics 75:2707-2720(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-29, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Venom.
  3. "Geographic variations in the composition of myotoxins from Bothrops neuwiedi snake venoms: biochemical characterization and biological activity."
    Rodrigues V.M., Soares A.M., Mancin A.C., Fontes M.R.M., Homsi-Brandeburgo M.I., Giglio J.R.
    Comp. Biochem. Physiol. 121A:215-222(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Tissue: Venom.
  4. "Insights of local tissue damage and regeneration induced by BnSP-7, a myotoxin isolated from Bothrops (neuwiedi) pauloensis snake venom."
    Oliveira C.F., da Silva Lopes D., Mendes M.M., Homsi-Brandeburgo M.I., Hamaguchi A., Machado de Alcantara T., Clissa P.B., de Melo Rodrigues V.
    Toxicon 53:560-569(2009)
    Cited for: FUNCTION.
    Tissue: Venom.
  5. "Crystallization and preliminary X-ray diffraction analysis of a myotoxic phospholipase A(2) homologue from Bothrops neuwiedi pauloensis venom."
    Fontes M.R.M., Soares A.M., Rodrigues V.M., Fernandes A.C., Da Silva R.J., Giglio J.R.
    Biochim. Biophys. Acta 1432:393-395(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CRYSTALLIZATION.
    Tissue: Venom.
  6. "Crystal structures of BnSP-7 and BnSP-6, two Lys49-phospholipases A2: quaternary structure and inhibition mechanism insights."
    Magro A.J., Soares A.M., Giglio J.R., Fontes M.R.M.
    Biochem. Biophys. Res. Commun. 311:713-720(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 1-119, VARIANT BNSP-6 GLY-34, SUBUNIT, DISULFIDE BONDS.

Entry informationi

Entry nameiPA2H_BOTPA
AccessioniPrimary (citable) accession number: Q9IAT9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: June 16, 2009
Last modified: May 11, 2016
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Does not bind calcium as one of the calcium-binding ligands is lost (Asp->Lys in position 46, which corresponds to 'Lys-49' in the current nomenclature).Curated

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.