Q9IAM7 (MRE11_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Double-strand break repair protein MRE11

Gene names

Name:

MRE11

Organism

Gallus gallus (Chicken) [Reference proteome]

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	700 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation By similarity.
Cofactor	Manganese By similarity.
Subunit structure	Component of the MRN complex composed of two heterodimers RAD50/MRE11A associated with a single NBN. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11A and NBN. Interacts with DCLRE1C/Artemis By similarity.
Subcellular location	Nucleus By similarity. Note: Localizes to discrete nuclear foci after treatment with genotoxic agents By similarity.
Sequence similarities	Belongs to the MRE11/RAD32 family.

Ontologies

Keywords
Biological process	DNA damage DNA repair Meiosis
Cellular component	Nucleus
Ligand	Manganese
Molecular function	Endonuclease Exonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	double-strand break repair via homologous recombination Traceable author statement. Source: Reactome meiosis Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Cellular_component	nucleoplasm Traceable author statement. Source: Reactome
Molecular_function	endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW exonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 700	700	Double-strand break repair protein MRE11		PRO_0000138676
Sites
	Active site	129	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9IAM7 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 53039A0FFEFE5292	FASTA	700	80,024
	10 20 30 40 50 60 MSAVSLQDDE DTFKILIATD IHLGYLEKDA VRGNDTFVTF NEILEHAQKN EVDFILLGGD 70 80 90 100 110 120 LFHENKPSRK TIHTCLESLR KYCMGDRPVS FEVLSDQAVN FQLSKFPWVN YQDENLNIFM 130 140 150 160 170 180 PIFSIHGNHD DPTGVDALCA LDILSCAGLL NHFGRSTSVE KIDISPILLR KGRTKIALYG 190 200 210 220 230 240 LGAIPDERLY RMFVNKQVTM LRPKEDEDSW FNMFVIHQNR SKHGATNYIP EQFLDDFINL 250 260 270 280 290 300 AVWGHEHECK ITPAQNEQQH FYVTQPGSSV VTSLSPGEAV KKHIGLLRVK GKKMKMQRIA 310 320 330 340 350 360 LETVRTFYME DVVLADHPEL FNPDNPKVTQ AIQAFCMEKV EMMLDNAERE RLGNPRQPQK 370 380 390 400 410 420 PLIILRVDYT GGFEPFIVHR FSQKYMDRVA NPKDIIHFFR HREQKEKNDN DINFGKLLSR 430 440 450 460 470 480 PASEEVTLRV EDLVKQYFQT AEKKVQLSLL TERRMGEAVQ EFVDKEEKDA IEELVKFQLE 490 500 510 520 530 540 KTQRFLKERH IDAEEEKIDE EVRKFRESRR KNTEEEDEEV REAMTRARAH RSEGVVLDSA 550 560 570 580 590 600 SSDEGLMDTG MKASGDSDDD IPTTLSRGRG RGRARGARGQ NSAARGSSRR GRGNTSQGSS 610 620 630 640 650 660 TSSRTYKSVP DKNSSIMDAF RSLKPEPSQS TSKFFSEDII DDEMDLEESP ISLSSKTNQR 670 680 690 700 SSAMSSFSKR GSQSQMSRGV DFESDEDDPF KNTATSRRKK

References

[1]

"Mre11 is essential for the maintenance of chromosomal DNA in vertebrate cells." Yamaguchi-Iwai Y., Sonoda E., Sasaki M.S., Morrison C., Haraguchi T., Hiraoka Y., Yamashita Y.M., Yagi T., Takata M., Price C., Kakazu N., Takeda S. EMBO J. 18:6619-6629(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF166094 mRNA. Translation: AAF31354.1.
IPI	IPI00599486.
RefSeq	NP_990109.1. NM_204778.1.
UniGene	Gga.3157.
3D structure databases
ProteinModelPortal	Q9IAM7.
ModBase	Search...
Protein-protein interaction databases
STRING	9031.ENSGALP00000035634.
Proteomic databases
PaxDb	Q9IAM7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	395555.
KEGG	gga:395555.
Organism-specific databases
CTD	4361.
Phylogenomic databases
eggNOG	COG0420.
HOGENOM	HOG000216581.
HOVERGEN	HBG052508.
KO	K10865.
OrthoDB	EOG46WZ82.
Enzyme and pathway databases
Reactome	REACT_115612. DNA replication and repair.
Family and domain databases
InterPro	IPR003701. DNA_repair_Mre11. IPR004843. Metallo_PEstase_dom. IPR007281. Mre11_DNA-bd. [Graphical view]
PANTHER	PTHR10139. PTHR10139. 1 hit.
Pfam	PF00149. Metallophos. 1 hit. PF04152. Mre11_DNA_bind. 1 hit. [Graphical view]
PIRSF	PIRSF000882. DSB_repair_MRE11. 1 hit.
TIGRFAMs	TIGR00583. mre11. 1 hit.
ProtoNet	Search...
Other
NextBio	20815630.

Entry information

Entry name

MRE11_CHICK

Accession

Primary (citable) accession number: Q9IAM7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	October 1, 2000
Last modified:	April 3, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program