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Protein

Mothers against decapentaplegic homolog 2

Gene

smad2

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Promotes differentiation of dorsal tissues. May be involved in the mediation of Ndr2 signaling during mesoderm and axis formation during embryogenesis.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi75ZincBy similarity1
Metal bindingi150ZincBy similarity1
Metal bindingi162ZincBy similarity1
Metal bindingi167ZincBy similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: ZFIN
  • sequence-specific DNA binding Source: ZFIN
  • transcription factor activity, sequence-specific DNA binding Source: UniProtKB

GO - Biological processi

  • embryonic pattern specification Source: UniProtKB
  • intracellular signal transduction Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: ZFIN
  • neural plate anterior/posterior regionalization Source: ZFIN
  • positive regulation of oocyte development Source: ZFIN
  • regulation of transcription, DNA-templated Source: ZFIN
  • regulation of transcription from RNA polymerase II promoter Source: ZFIN
  • transcription, DNA-templated Source: UniProtKB-KW
  • transforming growth factor beta receptor signaling pathway Source: InterPro

Keywordsi

Molecular functionDevelopmental protein, DNA-binding
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DRE-1181150. Signaling by NODAL.
R-DRE-1502540. Signaling by Activin.
R-DRE-2173788. Downregulation of TGF-beta receptor signaling.
R-DRE-2173789. TGF-beta receptor signaling activates SMADs.
R-DRE-2173795. Downregulation of SMAD2/3:SMAD4 transcriptional activity.
R-DRE-2173796. SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription.
R-DRE-5689880. Ub-specific processing proteases.

Names & Taxonomyi

Protein namesi
Recommended name:
Mothers against decapentaplegic homolog 2
Short name:
MAD homolog 2
Short name:
Mothers against DPP homolog 2
Alternative name(s):
SMAD family member 2
Short name:
SMAD 2
Short name:
Smad2
Gene namesi
Name:smad2
Synonyms:madh2
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 10

Organism-specific databases

ZFINiZDB-GENE-990603-7. smad2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000908551 – 468Mothers against decapentaplegic homolog 2Add BLAST468

Proteomic databases

PaxDbiQ9I9P9.

PTM databases

iPTMnetiQ9I9P9.

Expressioni

Developmental stagei

Expressed both maternally and zygotically. Expressed ubiquitously in the cleavage, blastula, gastrula and early somitogenesis stages. Expression declines during gastrulation. At 26 hours, expressed weakly throughout the head, and more strongly in the mesenchymal neural crest cells behind the eyes and in the endoderm and haemangiogenic region of the tail.2 Publications

Gene expression databases

BgeeiENSDARG00000006389.
ExpressionAtlasiQ9I9P9. baseline.

Interactioni

Protein-protein interaction databases

DIPiDIP-41211N.
IntActiQ9I9P9. 1 interactor.
MINTiMINT-230476.
STRINGi7955.ENSDARP00000111803.

Structurei

3D structure databases

ProteinModelPortaliQ9I9P9.
SMRiQ9I9P9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 177MH1PROSITE-ProRule annotationAdd BLAST168
Domaini275 – 468MH2PROSITE-ProRule annotationAdd BLAST194

Sequence similaritiesi

Belongs to the dwarfin/SMAD family.Curated

Phylogenomic databases

eggNOGiKOG3701. Eukaryota.
ENOG410XQKU. LUCA.
GeneTreeiENSGT00760000119091.
HOGENOMiHOG000286018.
HOVERGENiHBG053353.
InParanoidiQ9I9P9.
KOiK04500.
PhylomeDBiQ9I9P9.

Family and domain databases

Gene3Di2.60.200.10. 1 hit.
3.90.520.10. 1 hit.
InterProiView protein in InterPro
IPR013790. Dwarfin.
IPR003619. MAD_homology1_Dwarfin-type.
IPR013019. MAD_homology_MH1.
IPR017855. SMAD-like_dom_sf.
IPR001132. SMAD_dom_Dwarfin-type.
IPR008984. SMAD_FHA_dom_sf.
IPR036578. SMAD_MH1_sf.
PANTHERiPTHR13703. PTHR13703. 1 hit.
PfamiView protein in Pfam
PF03165. MH1. 1 hit.
PF03166. MH2. 1 hit.
SMARTiView protein in SMART
SM00523. DWA. 1 hit.
SM00524. DWB. 1 hit.
SUPFAMiSSF49879. SSF49879. 1 hit.
SSF56366. SSF56366. 2 hits.
PROSITEiView protein in PROSITE
PS51075. MH1. 1 hit.
PS51076. MH2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9I9P9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSILPFTPP VVKRLLGWKK SASGSSGAGG GGEQNGQEEK WCEKAVKSLV
60 70 80 90 100
KKLKKTGQLD ELEKAITTQN RNTKCVTIPS NCSEIWGLST PNTIEQWDTS
110 120 130 140 150
GLYSYPDQTR SLDGRLQVSH RKGLPHVIYC RLWRWPDLHS HHELRAIETC
160 170 180 190 200
EYAFNLKKDE VCVNPYHYQR VETPVLPPVL VPRHTEILTE LPPLDDYTNS
210 220 230 240 250
IPENTNFPTG IEPPNNYIPE TPPPGYISED GEASDQQMNQ SMDTGSPAEL
260 270 280 290 300
SPSTLSPVNH GMDLQPVTYS EPAFWCSIAY YELNQRVGET FHASQPSLTV
310 320 330 340 350
DGFTDPSNSE RFCLGLLSNV NRNATVEMTR RHIGRGVRLY YIGGEVFAEC
360 370 380 390 400
LSDSAIFVQS PNCNQRYGWH PATVCKIPPG CNLKIFNNQE FAALLAQSVN
410 420 430 440 450
QGFEAVYQLT RMCTIRMSFV KGWGAEYRRQ TVTSTPCWIE LHLNGPLQWL
460
DKVLTQMGSP SVRCSSMS
Length:468
Mass (Da):52,453
Last modified:October 1, 2000 - v1
Checksum:i464F4EFDF01D3270
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti315G → C in AAF06737 (PubMed:10525190).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF168774 mRNA. Translation: AAF06737.1.
AF229022 mRNA. Translation: AAF66239.1.
BC097043 mRNA. Translation: AAH97043.1.
RefSeqiNP_001276944.1. NM_001290015.1.
NP_571441.3. NM_131366.3.
UniGeneiDr.79140.

Genome annotation databases

EnsembliENSDART00000044756; ENSDARP00000044755; ENSDARG00000006389.
ENSDART00000128579; ENSDARP00000111803; ENSDARG00000006389.
GeneIDi30639.
KEGGidre:30639.

Similar proteinsi

Entry informationi

Entry nameiSMAD2_DANRE
AccessioniPrimary (citable) accession number: Q9I9P9
Secondary accession number(s): Q4V964, Q9PUN4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: October 1, 2000
Last modified: November 22, 2017
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families