Q9I9M2 (NOS_SQUAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Nitric oxide synthase Short name=NOS EC=1.14.13.39
Organism	Squalus acanthias (Spiny dogfish)
Taxonomic identifier	7797 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Chondrichthyes › Elasmobranchii › Selachii › Squalimorphii › Squaliformes › Squalidae › Squalus

Protein attributes

Sequence length	212 AA.
Sequence status	Fragment.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body By similarity.
Catalytic activity	2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.
Cofactor	Heme group By similarity. Binds 1 FAD By similarity. Binds 1 FMN By similarity.
Sequence similarities	Belongs to the NOS family. Contains 1 flavodoxin-like domain.

Ontologies

Keywords
Ligand	Calmodulin-binding FAD FMN Flavoprotein Heme Iron Metal-binding NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological_process	nitric oxide biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	FMN binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro nitric-oxide synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›212	›212	Nitric oxide synthase		PRO_0000170948
Regions
	Domain	60 – ›212	›153	Flavodoxin-like
	Nucleotide binding	186 – ›212	›27	FMN By similarity
	Region	30 – 50	21	Calmodulin-binding Potential
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	212	1

Sequences

	Sequence		Length	Mass (Da)
	Q9I9M2 [UniParc]. Last modified August 30, 2002. Version 2. Checksum: 3A6B115A679E6CFF	FASTA	212	23,651
	10 20 30 40 50 60 LNYQLSPSFE YQSDPWFTHV WKGVNGTPTK KRAIGFKKLA KAVKFSTKLM GQAMAKRVKA 70 80 90 100 110 120 TILYATETGK SQVYAKTLCE IFKHAFDAKV MSMDEYDIVH LEHEALVLVV TSTFGNGDPP 130 140 150 160 170 180 ENGEKFGSAL MEIRHPSSNS AERKSYKVRF NSVSSYSDSR KSSSDEPEHK DNFESTGPLA 190 200 210 NVRFSAFGLG SRAYPHFCAF ARAVDTLLEE LG

References

[1]	Farmerie W.G., Holland K.P., Catches J.S., Morrison-Shetlar A.I., Claiborne J.B. III, Edwards S.L., Evans D.H. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Gill.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF232227 mRNA. Translation: AAF36405.2.
3D structure databases
ProteinModelPortal	Q9I9M2.
SMR	Q9I9M2. Positions 55-212.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001094. Flavdoxin. IPR008254. Flavodoxin/NO_synth. IPR026009. NOS. [Graphical view]
PANTHER	PTHR19384:SF5. PTHR19384:SF5. 1 hit.
Pfam	PF00258. Flavodoxin_1. 1 hit. [Graphical view]
PRINTS	PR00369. FLAVODOXIN.
PROSITE	PS50902. FLAVODOXIN_LIKE. 1 hit. PS60001. NOS. Partial match. PS50106. PDZ. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NOS_SQUAC

Accession

Primary (citable) accession number: Q9I9M2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	August 30, 2002
Last modified:	November 28, 2012
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program