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Protein

Nitric oxide synthase

Gene
N/A
Organism
Squalus acanthias (Spiny dogfish)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body.By similarity

Catalytic activityi

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactori

Protein has several cofactor binding sites:
  • hemeBy similarity
  • FADBy similarityNote: Binds 1 FAD.By similarity
  • FMNBy similarityNote: Binds 1 FMN.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi186 – ›212FMNPROSITE-ProRule annotationAdd BLAST›27

GO - Molecular functioni

Keywordsi

Molecular functionCalmodulin-binding, Oxidoreductase
LigandFAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Nitric oxide synthase (EC:1.14.13.39)
Short name:
NOS
OrganismiSqualus acanthias (Spiny dogfish)
Taxonomic identifieri7797 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataChondrichthyesElasmobranchiiSqualimorphiiSqualiformesSqualidaeSqualus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000170948‹1 – ›212Nitric oxide synthaseAdd BLAST›212

Interactioni

GO - Molecular functioni

Structurei

3D structure databases

ProteinModelPortaliQ9I9M2.
SMRiQ9I9M2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini60 – ›212Flavodoxin-likePROSITE-ProRule annotationAdd BLAST›153

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni30 – 50Calmodulin-bindingSequence analysisAdd BLAST21

Sequence similaritiesi

Belongs to the NOS family.Curated

Family and domain databases

Gene3Di3.40.50.360. 1 hit.
InterProiView protein in InterPro
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR029039. Flavoprotein-like_sf.
PfamiView protein in Pfam
PF00258. Flavodoxin_1. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
SUPFAMiSSF52218. SSF52218. 1 hit.
PROSITEiView protein in PROSITE
PS50902. FLAVODOXIN_LIKE. 1 hit.

Sequencei

Sequence statusi: Fragment.

Q9I9M2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
LNYQLSPSFE YQSDPWFTHV WKGVNGTPTK KRAIGFKKLA KAVKFSTKLM
60 70 80 90 100
GQAMAKRVKA TILYATETGK SQVYAKTLCE IFKHAFDAKV MSMDEYDIVH
110 120 130 140 150
LEHEALVLVV TSTFGNGDPP ENGEKFGSAL MEIRHPSSNS AERKSYKVRF
160 170 180 190 200
NSVSSYSDSR KSSSDEPEHK DNFESTGPLA NVRFSAFGLG SRAYPHFCAF
210
ARAVDTLLEE LG
Length:212
Mass (Da):23,651
Last modified:August 30, 2002 - v2
Checksum:i3A6B115A679E6CFF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11
Non-terminal residuei2121

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF232227 mRNA. Translation: AAF36405.2.

Similar proteinsi

Entry informationi

Entry nameiNOS_SQUAC
AccessioniPrimary (citable) accession number: Q9I9M2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: August 30, 2002
Last modified: November 22, 2017
This is version 85 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families