Reviewed, UniProtKB/Swiss-Prot Q9I9M2 (NOS_SQUAC)

Names and origin

Protein names	Recommended name:     Nitric oxide synthase       Short name=NOS     EC=1.14.13.39
Organism	Squalus acanthias (Spiny dogfish)
Taxonomic identifier	7797 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Chondrichthyes › Elasmobranchii › Squalea › Hypnosqualea › Squaliformes › Squaloidei › Squalidae › Squalus

Protein attributes

Sequence length	212 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body By similarity.
Catalytic activity	L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+.
Cofactor	Heme group By similarity. Binds 1 FAD By similarity. Binds 1 FMN By similarity.
Sequence similarities	Belongs to the NOS family. Contains 1 flavodoxin-like domain.

Ontologies

Keywords
Ligand	Calmodulin-binding FAD FMN Flavoprotein Heme Iron Metal-binding NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	nitric oxide biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro calmodulin binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitric-oxide synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›212	›212	Nitric oxide synthase		PRO_0000170948
Regions
	Domain	60 – ›212	›153	Flavodoxin-like
	Nucleotide binding	186 – ›212	›27	FMN By similarity
	Region	30 – 50	21	Calmodulin-binding Potential
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	212	1

Sequences

	Sequence		Length	Mass (Da)
	Q9I9M2-1 [UniParc]. Last modified August 30, 2002. Version 2. Checksum: 3A6B115A679E6CFF	FASTA	212	23,651
	10 20 30 40 50 60 LNYQLSPSFE YQSDPWFTHV WKGVNGTPTK KRAIGFKKLA KAVKFSTKLM GQAMAKRVKA 70 80 90 100 110 120 TILYATETGK SQVYAKTLCE IFKHAFDAKV MSMDEYDIVH LEHEALVLVV TSTFGNGDPP 130 140 150 160 170 180 ENGEKFGSAL MEIRHPSSNS AERKSYKVRF NSVSSYSDSR KSSSDEPEHK DNFESTGPLA 190 200 210 NVRFSAFGLG SRAYPHFCAF ARAVDTLLEE LG

References

[1]	Farmerie W.G., Holland K.P., Catches J.S., Morrison-Shetlar A.I., Claiborne J.B. III, Edwards S.L., Evans D.H. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Gill.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF232227 mRNA. Translation: AAF36405.2.
3D structure databases
HSSP	HSSP built from PDB template 1NOC based on UniProtKB P29477.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q9I9M2.
Enzyme and pathway databases
BRENDA	1.14.13.39. 981.
Family and domain databases
InterPro	IPR001094. Flavdoxin-like. IPR008254. Flavodoxin/NO_synth. IPR004030. NO_synthase_oxygenase_reg. [Graphical view]
Pfam	PF00258. Flavodoxin_1. 1 hit. PF02898. NO_synthase. 1 hit. [Graphical view]
PRINTS	PR00369. FLAVODOXIN.
PROSITE	PS50902. FLAVODOXIN_LIKE. 1 hit. PS60001. NOS. Partial match. PS50106. PDZ. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NOS_SQUAC

Accession

Primary (citable) accession number: Q9I9M2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	August 30, 2002
Last modified:	June 16, 2009
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)