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Protein

Apoptotic protease-activating factor 1

Gene

apaf1

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi154 – 1618ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Apoptosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Apoptotic protease-activating factor 1
Short name:
APAF-1
Gene namesi
Name:apaf1
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Unplaced

Organism-specific databases

ZFINiZDB-GENE-000616-4. apaf1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12611261Apoptotic protease-activating factor 1PRO_0000050847Add
BLAST

Proteomic databases

PaxDbiQ9I9H8.

Interactioni

Subunit structurei

Monomer. Oligomerizes upon binding of cytochrome c and dATP (By similarity).By similarity

Protein-protein interaction databases

STRINGi7955.ENSDARP00000105810.

Structurei

3D structure databases

ProteinModelPortaliQ9I9H8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 9090CARDPROSITE-ProRule annotationAdd
BLAST
Domaini106 – 417312NB-ARCAdd
BLAST
Repeati615 – 65440WD 1Add
BLAST
Repeati657 – 69640WD 2Add
BLAST
Repeati700 – 74344WD 3Add
BLAST
Repeati746 – 78540WD 4Add
BLAST
Repeati798 – 83639WD 5Add
BLAST
Repeati840 – 87940WD 6Add
BLAST
Repeati882 – 92140WD 7Add
BLAST
Repeati964 – 100340WD 8Add
BLAST
Repeati1006 – 104540WD 9Add
BLAST
Repeati1047 – 108842WD 10Add
BLAST
Repeati1091 – 113040WD 11Add
BLAST
Repeati1133 – 117240WD 12Add
BLAST
Repeati1184 – 122340WD 13Add
BLAST

Sequence similaritiesi

Contains 1 CARD domain.PROSITE-ProRule annotation
Contains 1 NB-ARC domain.Curated
Contains 13 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG4155. Eukaryota.
KOG4658. Eukaryota.
ENOG410ZW6V. LUCA.
HOGENOMiHOG000290173.
HOVERGENiHBG018730.
InParanoidiQ9I9H8.
KOiK02084.
PhylomeDBiQ9I9H8.

Family and domain databases

Gene3Di1.10.533.10. 1 hit.
2.130.10.10. 2 hits.
InterProiIPR017251. Apaf-1.
IPR001315. CARD.
IPR011029. DEATH-like_dom.
IPR020472. G-protein_beta_WD-40_rep.
IPR002182. NB-ARC.
IPR027417. P-loop_NTPase.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00619. CARD. 1 hit.
PF00931. NB-ARC. 1 hit.
PF00400. WD40. 7 hits.
[Graphical view]
PIRSFiPIRSF037646. Apop_pept_activating-1. 1 hit.
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 14 hits.
[Graphical view]
SUPFAMiSSF47986. SSF47986. 1 hit.
SSF50978. SSF50978. 2 hits.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50209. CARD. 1 hit.
PS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 7 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9I9H8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEERARSRLL RSKATLEQDI KASYLMDHMI SDGVLTNDEE AKVLSKATRK
60 70 80 90 100
EQAVALLETL LRKDNRAYIS FYNALIRESY GDLASLLHSD LPLLSPEGEK
110 120 130 140 150
SFADGVSPSV QAILSVGGVP QRPVVFVSRP PLLNLIREML YQLRDTPGWV
160 170 180 190 200
TVFGMAGSGK SVMAAEVVRD RSLIKECFPD GVHWLSVGQC ERADLLVRMQ
210 220 230 240 250
SLCFRLEQCQ SSDTSQRPPS TVEEAKERLR FLMLRRFPRS LLILDDVWDS
260 270 280 290 300
SSLRSFDIQC RVLLTTRNRA LTDSVSGVRY EVPVENGLDE EKALEILALY
310 320 330 340 350
VNGKMHKLPE QARSIVSECK GSPLVVSLIG ALLREFPDRW SYYLRQLQQK
360 370 380 390 400
QFKRIRKSSS YDYEALDQAM DASLQVLEAE HQELYRDLSV MQKDIKVPAK
410 420 430 440 450
VLSVLWGLEL EEVEDVLQEF VNKSLLFRDC NQRPYRYYLH DLQLDFLAEQ
460 470 480 490 500
NRDQIAELHK KMVRQYQRFY SKRPPDSADK DSLYWYQFIP YHMAKAGLSK
510 520 530 540 550
ELYSLMFSLD WVKEKARIMG SAHLINDYVE YGEILDKENS EVRVQFQEFL
560 570 580 590 600
SLNGHHLEQR PFPDVVQLAL SQPDRSEVYR QALMQAQKRA SRGQIYLNWV
610 620 630 640 650
NKNIEEGLSR LVMHPHQGAV YYACFSKDGS KIASCGASKA LRVFKSTSGE
660 670 680 690 700
KLLELQAHEE DVLCCAFSPD DRHIATCASD RKVKLWNVER GVLIREFEVE
710 720 730 740 750
HEEQINHCQF TNTGRRVLLA TCSNDKFTNT RLWNPNKKTS QNTMFGHMEP
760 770 780 790 800
VNHCCFSPND LYLATSSSDG SLKLFEVSSA NEWKSIDVDS FFPESDEEIK
810 820 830 840 850
AMVKCSTWSA DGSQIICAAR NTVFVFDVET SDLLLKLKTS RLSTIQFCHA
860 870 880 890 900
CPNSSLLAVA LSHYTVELWN FESSKKKAEC SGHLSWVHCV QFSPDGSLLL
910 920 930 940 950
SSSDDQTIRL WETDRVHTSS AVALKRDTDV LSSHSDATII APDSSNRLQV
960 970 980 990 1000
LSGSTGAVVL ESEELSSRIR CSCISRNAAF VALGSEDGTV QVIEVPSSKA
1010 1020 1030 1040 1050
SVKLSGHTKT VHHCQFTDDC EILITSSEDS TIRVWKWRTG ECMVLQGHME
1060 1070 1080 1090 1100
PVRKFHLLSS SSSPHLFSWS FDGTVKVWDL TRGQMLQDLV CHEGAVLSCD
1110 1120 1130 1140 1150
VSSDGRLFAT TSANRTAKVW SSASWKMLFL LEGHKDCVRS CRFSWDNKRL
1160 1170 1180 1190 1200
ATGDDNGEIR LWSMLDGALL KICPRDTKDS MNSYHAGWVT DLHFSPDNRV
1210 1220 1230 1240 1250
LVSTAGYIKW WSVESGEALQ TFYTMGGNLK KIHVSPDFST FITVDSIGIL
1260
YILKRLEGEG T
Length:1,261
Mass (Da):142,934
Last modified:October 1, 2000 - v1
Checksum:iF80CC9CB41764F1C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF251502 mRNA. Translation: AAF67189.1.
RefSeqiNP_571683.1. NM_131608.1.
UniGeneiDr.78833.

Genome annotation databases

GeneIDi58131.
KEGGidre:58131.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF251502 mRNA. Translation: AAF67189.1.
RefSeqiNP_571683.1. NM_131608.1.
UniGeneiDr.78833.

3D structure databases

ProteinModelPortaliQ9I9H8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000105810.

Proteomic databases

PaxDbiQ9I9H8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi58131.
KEGGidre:58131.

Organism-specific databases

CTDi317.
ZFINiZDB-GENE-000616-4. apaf1.

Phylogenomic databases

eggNOGiKOG4155. Eukaryota.
KOG4658. Eukaryota.
ENOG410ZW6V. LUCA.
HOGENOMiHOG000290173.
HOVERGENiHBG018730.
InParanoidiQ9I9H8.
KOiK02084.
PhylomeDBiQ9I9H8.

Miscellaneous databases

PROiQ9I9H8.

Family and domain databases

Gene3Di1.10.533.10. 1 hit.
2.130.10.10. 2 hits.
InterProiIPR017251. Apaf-1.
IPR001315. CARD.
IPR011029. DEATH-like_dom.
IPR020472. G-protein_beta_WD-40_rep.
IPR002182. NB-ARC.
IPR027417. P-loop_NTPase.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00619. CARD. 1 hit.
PF00931. NB-ARC. 1 hit.
PF00400. WD40. 7 hits.
[Graphical view]
PIRSFiPIRSF037646. Apop_pept_activating-1. 1 hit.
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 14 hits.
[Graphical view]
SUPFAMiSSF47986. SSF47986. 1 hit.
SSF50978. SSF50978. 2 hits.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50209. CARD. 1 hit.
PS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 7 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genes with homology to mammalian apoptosis regulators identified in zebrafish."
    Inohara N., Nunez G.
    Cell Death Differ. 7:509-510(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiAPAF_DANRE
AccessioniPrimary (citable) accession number: Q9I9H8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: October 1, 2000
Last modified: June 8, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.