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Protein

Forkhead box protein H1

Gene

foxh1

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator. Activates an activin response element (ARE). Recognizes and binds to the DNA sequence 5'-TGT[GT][GT]ATT-3'. Modulator of nodal signaling required for organizer formation. Also required for the development of dorsal axial structures and left-right symmetry.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi97 – 193Fork-headPROSITE-ProRule annotationAdd BLAST97

GO - Molecular functioni

  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: ZFIN
  • sequence-specific DNA binding Source: ZFIN
  • SMAD binding Source: ZFIN

GO - Biological processi

  • axial mesodermal cell fate specification Source: ZFIN
  • axial mesoderm morphogenesis Source: ZFIN
  • blood vessel development Source: ZFIN
  • canonical Wnt signaling pathway Source: ZFIN
  • cell migration involved in gastrulation Source: ZFIN
  • determination of left/right symmetry Source: ZFIN
  • dorsal/ventral pattern formation Source: ZFIN
  • embryonic heart tube left/right pattern formation Source: ZFIN
  • embryonic heart tube morphogenesis Source: ZFIN
  • endoderm formation Source: ZFIN
  • floor plate development Source: ZFIN
  • floor plate formation Source: ZFIN
  • heart looping Source: ZFIN
  • mesendoderm development Source: ZFIN
  • mesoderm formation Source: ZFIN
  • negative regulation of transcription from RNA polymerase II promoter Source: ZFIN
  • notochord development Source: ZFIN
  • notochord formation Source: ZFIN
  • optic placode formation involved in camera-type eye formation Source: ZFIN
  • prechordal plate formation Source: ZFIN
  • regulation of BMP signaling pathway involved in heart jogging Source: ZFIN
  • regulation of gene expression Source: ZFIN
  • regulation of transcription from RNA polymerase II promoter Source: ZFIN
  • somite rostral/caudal axis specification Source: ZFIN
  • specification of animal organ position Source: ZFIN
  • Spemann organizer formation at the embryonic shield Source: ZFIN
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Forkhead box protein H1
Alternative name(s):
Forkhead activin signal transducer 1
Short name:
Fast-1
Schmalspur protein
Gene namesi
Name:foxh1
Synonyms:fast1, sur
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Unplaced

Organism-specific databases

ZFINiZDB-GENE-000616-15. foxh1.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Fishes exhibit ventral body curvature, reduced spacing of the eyes, and absence or reduction of ventral neuroectoderm, including the floor plate.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi94R → H in sur(m768); loss of function. 2 Publications1
Mutagenesisi97K → N in sur(ty68b); loss of function. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000918441 – 472Forkhead box protein H1Add BLAST472

Proteomic databases

PaxDbiQ9I9E1.

Expressioni

Developmental stagei

Expressed both maternally and zygotically. Localized to the prospective animal pole during oogenesis. Ubiquitous expression during the blastula stages. During early gastrulation, levels are higher ventrally and in the presumptive shield (organizer region). Later in gastrulation, expression is restricted to midline and ventral cells. At the start of somatogenesis, expression is restricted to notochord, lateral plate mesoderm and a stripe of anterior dorsal neuroectoderm. By the 19-somite stage, expression in the lateral plate and midline diminishes but forebrain expression persists. All expression is lost by 24 hours post-fertilization (hpf).3 Publications

Interactioni

GO - Molecular functioni

  • SMAD binding Source: ZFIN

Protein-protein interaction databases

STRINGi7955.ENSDARP00000072485.

Structurei

3D structure databases

ProteinModelPortaliQ9I9E1.
SMRiQ9I9E1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni339 – 465SMAD-interaction domain (SID)Add BLAST127

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi357 – 361Fast/FoxH1 motif 1 (FM1)5
Motifi367 – 373Fast/FoxH1 motif 2 (FM2)7
Motifi428 – 448SMAD interaction motif (SIM)Add BLAST21

Domaini

The FM region is required for binding smad2/smad4 complexes. FM2 is more effective than FM1 and only interacts with phosphorylated smad2 that is in an activated smad complex (By similarity).By similarity

Sequence similaritiesi

Contains 1 fork-head DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2294. Eukaryota.
COG5025. LUCA.
HOGENOMiHOG000169991.
HOVERGENiHBG051646.
InParanoidiQ9I9E1.
KOiK09400.
PhylomeDBiQ9I9E1.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR001766. Fork_head_dom.
IPR030456. TF_fork_head_CS_2.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00250. Forkhead. 1 hit.
[Graphical view]
PRINTSiPR00053. FORKHEAD.
SMARTiSM00339. FH. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS00658. FORK_HEAD_2. 1 hit.
PS50039. FORK_HEAD_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9I9E1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKHWGGPGL LAPPVITVGE GAQRDHHLDC RIGYSSSKRS CHRSSNPLLE
60 70 80 90 100
LGGRLDKSTG MAQDSCYRAK ATNQGPWELQ DGNSSGGKKK NYQRYPKPPY
110 120 130 140 150
SYLAMIAMVI QNSPEKKLTL SEILKEISTL FPFFKGNYKG WRDSVRHNLS
160 170 180 190 200
SYDCFVKVLK DPGKPQGKGN FWTVEVNRIP LELLKRQNTA VSRQDETIFA
210 220 230 240 250
QDLAPYIFQG YSQPNKSKPL PPESSLPPVP TRQSPPPSED PYRPKLDSTF
260 270 280 290 300
AIDSLLHSLR PASSAGEGLR ERESWGVGPP PHTRSTTPPR PCNASYNGSS
310 320 330 340 350
SASSVSPASD FSDEDWRGVT VVGKRSGDRG ITSDAYSDSC PPPNKSSKRG
360 370 380 390 400
NTPPWELPTS YAKYTPPNAV APPSMRFNGN PFMPLGGIPF YGYGGAHVTT
410 420 430 440 450
SHLIGHPYWP ILPSGPVSIQ APPLLMDLDS MLQSVPPNKS VFDALGSNNQ
460 470
TVHPSPNQYA LQNGPSLCKY SL
Length:472
Mass (Da):51,620
Last modified:October 1, 2000 - v1
Checksum:iEC6285F1BFA970CA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti233Q → H in AAF81247 (PubMed:11091091).Curated1
Sequence conflicti318G → R in AAF81247 (PubMed:11091091).Curated1
Sequence conflicti395G → S in AAF81247 (PubMed:11091091).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF263000 mRNA. Translation: AAF72712.1.
AF264751 mRNA. Translation: AAF81247.1.
BC044340 mRNA. Translation: AAH44340.1.
RefSeqiNP_571577.1. NM_131502.1.
UniGeneiDr.7743.

Genome annotation databases

GeneIDi57930.
KEGGidre:57930.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF263000 mRNA. Translation: AAF72712.1.
AF264751 mRNA. Translation: AAF81247.1.
BC044340 mRNA. Translation: AAH44340.1.
RefSeqiNP_571577.1. NM_131502.1.
UniGeneiDr.7743.

3D structure databases

ProteinModelPortaliQ9I9E1.
SMRiQ9I9E1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000072485.

Proteomic databases

PaxDbiQ9I9E1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi57930.
KEGGidre:57930.

Organism-specific databases

CTDi8928.
ZFINiZDB-GENE-000616-15. foxh1.

Phylogenomic databases

eggNOGiKOG2294. Eukaryota.
COG5025. LUCA.
HOGENOMiHOG000169991.
HOVERGENiHBG051646.
InParanoidiQ9I9E1.
KOiK09400.
PhylomeDBiQ9I9E1.

Miscellaneous databases

PROiQ9I9E1.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR001766. Fork_head_dom.
IPR030456. TF_fork_head_CS_2.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00250. Forkhead. 1 hit.
[Graphical view]
PRINTSiPR00053. FORKHEAD.
SMARTiSM00339. FH. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS00658. FORK_HEAD_2. 1 hit.
PS50039. FORK_HEAD_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFOXH1_DANRE
AccessioniPrimary (citable) accession number: Q9I9E1
Secondary accession number(s): Q9I8K6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 14, 2001
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.