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Protein

Forkhead box protein H1

Gene

foxh1

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional activator. Activates an activin response element (ARE). Recognizes and binds to the DNA sequence 5'-TGT[GT][GT]ATT-3'. Modulator of nodal signaling required for organizer formation. Also required for the development of dorsal axial structures and left-right symmetry.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi97 – 193Fork-headPROSITE-ProRule annotationAdd BLAST97

GO - Molecular functioni

  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: ZFIN
  • sequence-specific DNA binding Source: ZFIN
  • SMAD binding Source: ZFIN
  • transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding Source: GO_Central

GO - Biological processi

  • axial mesodermal cell fate specification Source: ZFIN
  • axial mesoderm morphogenesis Source: ZFIN
  • blood vessel development Source: ZFIN
  • brain morphogenesis Source: ZFIN
  • canonical Wnt signaling pathway Source: ZFIN
  • cell migration involved in gastrulation Source: ZFIN
  • cellular response to cytokine stimulus Source: UniProtKB
  • determination of left/right symmetry Source: ZFIN
  • dorsal/ventral pattern formation Source: ZFIN
  • embryonic heart tube left/right pattern formation Source: ZFIN
  • embryonic heart tube morphogenesis Source: ZFIN
  • endoderm formation Source: ZFIN
  • floor plate development Source: ZFIN
  • floor plate formation Source: ZFIN
  • heart looping Source: ZFIN
  • hypothalamus development Source: ZFIN
  • mesendoderm development Source: ZFIN
  • mesoderm formation Source: ZFIN
  • negative regulation of transcription by RNA polymerase II Source: ZFIN
  • notochord development Source: ZFIN
  • notochord formation Source: ZFIN
  • optic placode formation involved in camera-type eye formation Source: ZFIN
  • positive regulation of transcription by RNA polymerase II Source: UniProtKB
  • prechordal plate formation Source: ZFIN
  • regulation of BMP signaling pathway involved in heart jogging Source: ZFIN
  • regulation of gene expression Source: ZFIN
  • regulation of transcription by RNA polymerase II Source: ZFIN
  • somite rostral/caudal axis specification Source: ZFIN
  • specification of animal organ position Source: ZFIN
  • Spemann organizer formation at the embryonic shield Source: ZFIN
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Forkhead box protein H1
Alternative name(s):
Forkhead activin signal transducer 1
Short name:
Fast-1
Schmalspur protein
Gene namesi
Name:foxh1
Synonyms:fast1, sur
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Unplaced

Organism-specific databases

ZFINiZDB-GENE-000616-15 foxh1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Fishes exhibit ventral body curvature, reduced spacing of the eyes, and absence or reduction of ventral neuroectoderm, including the floor plate.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi94R → H in sur(m768); loss of function. 2 Publications1
Mutagenesisi97K → N in sur(ty68b); loss of function. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000918441 – 472Forkhead box protein H1Add BLAST472

Proteomic databases

PaxDbiQ9I9E1

Expressioni

Developmental stagei

Expressed both maternally and zygotically. Localized to the prospective animal pole during oogenesis. Ubiquitous expression during the blastula stages. During early gastrulation, levels are higher ventrally and in the presumptive shield (organizer region). Later in gastrulation, expression is restricted to midline and ventral cells. At the start of somatogenesis, expression is restricted to notochord, lateral plate mesoderm and a stripe of anterior dorsal neuroectoderm. By the 19-somite stage, expression in the lateral plate and midline diminishes but forebrain expression persists. All expression is lost by 24 hours post-fertilization (hpf).3 Publications

Interactioni

GO - Molecular functioni

  • SMAD binding Source: ZFIN

Protein-protein interaction databases

STRINGi7955.ENSDARP00000072485

Structurei

3D structure databases

ProteinModelPortaliQ9I9E1
SMRiQ9I9E1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni339 – 465SMAD-interaction domain (SID)Add BLAST127

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi357 – 361Fast/FoxH1 motif 1 (FM1)5
Motifi367 – 373Fast/FoxH1 motif 2 (FM2)7
Motifi428 – 448SMAD interaction motif (SIM)Add BLAST21

Domaini

The FM region is required for binding smad2/smad4 complexes. FM2 is more effective than FM1 and only interacts with phosphorylated smad2 that is in an activated smad complex (By similarity).By similarity

Phylogenomic databases

eggNOGiKOG2294 Eukaryota
COG5025 LUCA
HOGENOMiHOG000169991
HOVERGENiHBG051646
InParanoidiQ9I9E1
KOiK09400
PhylomeDBiQ9I9E1

Family and domain databases

CDDicd00059 FH, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR001766 Fork_head_dom
IPR030456 TF_fork_head_CS_2
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00250 Forkhead, 1 hit
PRINTSiPR00053 FORKHEAD
SMARTiView protein in SMART
SM00339 FH, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS00658 FORK_HEAD_2, 1 hit
PS50039 FORK_HEAD_3, 1 hit

Sequencei

Sequence statusi: Complete.

Q9I9E1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKHWGGPGL LAPPVITVGE GAQRDHHLDC RIGYSSSKRS CHRSSNPLLE
60 70 80 90 100
LGGRLDKSTG MAQDSCYRAK ATNQGPWELQ DGNSSGGKKK NYQRYPKPPY
110 120 130 140 150
SYLAMIAMVI QNSPEKKLTL SEILKEISTL FPFFKGNYKG WRDSVRHNLS
160 170 180 190 200
SYDCFVKVLK DPGKPQGKGN FWTVEVNRIP LELLKRQNTA VSRQDETIFA
210 220 230 240 250
QDLAPYIFQG YSQPNKSKPL PPESSLPPVP TRQSPPPSED PYRPKLDSTF
260 270 280 290 300
AIDSLLHSLR PASSAGEGLR ERESWGVGPP PHTRSTTPPR PCNASYNGSS
310 320 330 340 350
SASSVSPASD FSDEDWRGVT VVGKRSGDRG ITSDAYSDSC PPPNKSSKRG
360 370 380 390 400
NTPPWELPTS YAKYTPPNAV APPSMRFNGN PFMPLGGIPF YGYGGAHVTT
410 420 430 440 450
SHLIGHPYWP ILPSGPVSIQ APPLLMDLDS MLQSVPPNKS VFDALGSNNQ
460 470
TVHPSPNQYA LQNGPSLCKY SL
Length:472
Mass (Da):51,620
Last modified:October 1, 2000 - v1
Checksum:iEC6285F1BFA970CA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti233Q → H in AAF81247 (PubMed:11091091).Curated1
Sequence conflicti318G → R in AAF81247 (PubMed:11091091).Curated1
Sequence conflicti395G → S in AAF81247 (PubMed:11091091).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF263000 mRNA Translation: AAF72712.1
AF264751 mRNA Translation: AAF81247.1
BC044340 mRNA Translation: AAH44340.1
RefSeqiNP_571577.1, NM_131502.1
UniGeneiDr.7743

Genome annotation databases

GeneIDi57930
KEGGidre:57930

Similar proteinsi

Entry informationi

Entry nameiFOXH1_DANRE
AccessioniPrimary (citable) accession number: Q9I9E1
Secondary accession number(s): Q9I8K6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 14, 2001
Last sequence update: October 1, 2000
Last modified: March 28, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health