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Q9I958

- MK14B_CYPCA

UniProt

Q9I958 - MK14B_CYPCA

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Protein

Mitogen-activated protein kinase 14B

Gene

mapk14b

Organism
Cyprinus carpio (Common carp)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Mapk14b is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets By similarity.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Cofactori

Magnesium.1 Publication

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation by the dual specificity kinase, MKK6.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei54 – 541ATPPROSITE-ProRule annotation
Active sitei151 – 1511Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi31 – 399ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: UniProtKB

GO - Biological processi

  1. intracellular signal transduction Source: UniProtKB
  2. p38MAPK cascade Source: UniProtKB
  3. positive regulation of myoblast differentiation Source: UniProtKB
  4. positive regulation of myoblast fusion Source: UniProtKB
  5. positive regulation of myotube differentiation Source: UniProtKB
  6. regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  7. response to stress Source: UniProtKB
  8. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Stress response, Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.24. 1195.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 14B (EC:2.7.11.24)
Short name:
MAP kinase 14B
Short name:
MAPK 14B
Alternative name(s):
Mitogen-activated protein kinase p38b
Short name:
MAP kinase p38b
Short name:
cp38b
Gene namesi
Name:mapk14b
OrganismiCyprinus carpio (Common carp)Imported
Taxonomic identifieri7962 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeCyprinus

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 361361Mitogen-activated protein kinase 14BPRO_0000186298Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei181 – 1811Phosphothreonine; by MAP2K61 Publication
Modified residuei183 – 1831Phosphotyrosine; by MAP2K61 Publication

Post-translational modificationi

Dually phosphorylated on Thr-181 and Tyr-183, which activates the enzyme.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ9I958.

Expressioni

Tissue specificityi

Predominantly expressed in the ovary. Lower levels present in brain, gill, heart, spleen, kidney, muscle and gut.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ9I958.
SMRiQ9I958. Positions 5-352.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 309285Protein kinasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi181 – 1833TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG014652.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008352. MAPK_p38.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01773. P38MAPKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9I958-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSHKERPTFY RQELNKTIWE VPERYQNLSP VGSGAYGSVC SALDTKSGLR
60 70 80 90 100
VAVKKLSRPF QSMIHAKRTY RELRLLKHMK HENVIGLLDA FSPATCLAGF
110 120 130 140 150
NDVYLVTHLM GADLNNIVKC QKLTDDHVQF LIYQILRGLK YIHSADIIHR
160 170 180 190 200
DLKPSNLAVN EDCELKILDF GLARLTDDEM TGYVATRWYR APEIMLNWMH
210 220 230 240 250
YNMTVDIWSV GCIMAELLTG RTLFPGTDHI NQLQQIMRLT GTPPASLISR
260 270 280 290 300
MPSHEARNYI NSLSYMPKRN FADVFVGANP MAVDLLEKML VLDTDKRITA
310 320 330 340 350
SQALAHPYFA QYHDPDDEPE ADPYDQSFES RDLDIEEWKR LTYEEVISFE
360
PPVFDGDEME S
Length:361
Mass (Da):41,484
Last modified:October 1, 2000 - v1
Checksum:iCBE14921A49638BD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB023481 mRNA. Translation: BAA96415.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB023481 mRNA. Translation: BAA96415.1 .

3D structure databases

ProteinModelPortali Q9I958.
SMRi Q9I958. Positions 5-352.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q9I958.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOVERGENi HBG014652.

Enzyme and pathway databases

BRENDAi 2.7.11.24. 1195.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008352. MAPK_p38.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
PRINTSi PR01773. P38MAPKINASE.
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Identification of a nuclear export signal in MKK6, an activator of the carp p38 mitogen-activated protein kinases."
    Hashimoto H., Fukuda M., Matsuo Y., Yokoyama Y., Nishida E., Toyohara H., Sakaguchi M.
    Eur. J. Biochem. 267:4362-4371(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], COFACTOR, TISSUE SPECIFICITY, PHOSPHORYLATION, ENZYME REGULATION.
    Tissue: Ovary.

Entry informationi

Entry nameiMK14B_CYPCA
AccessioniPrimary (citable) accession number: Q9I958
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2002
Last sequence update: October 1, 2000
Last modified: October 29, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3